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OfflineGoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
Safe and Sterile inoculation Methods
    #22385941 - 10/15/15 10:03 PM (8 years, 3 months ago)

Hello all, I was curious to hear what the communities opinion is the "best" inoculation methods; i.e. still air chambers, glove-boxes, hood's, tents, etc.  I am interested in anything yall have had success with and what seems to work the best for you.  Hopefully we can all learn.  Thanks for so much help, Miles Davis.


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Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money.  But hell life's right in front of me, nothing to look forward to.  Best of luck to all.


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Safe and Sterile inoculation Methods [Re: GoldenGrowBoy]
    #22385965 - 10/15/15 10:07 PM (8 years, 3 months ago)

Agar. And you can be pretty sloppy on initial inoculation with agar and still get a clean culture. Sabs are the best IMO. FH is so easy, but a lot of agar work (spores and contams, the main reasons to use agar) should still be done in a sab.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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InvisibleGoblinKing
Trismegistus

Registered: 08/17/15
Posts: 328
Loc: Right where it belongs.
Re: Safe and Sterile inoculation Methods [Re: Mad Season]
    #22385985 - 10/15/15 10:12 PM (8 years, 3 months ago)



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OfflineDarkhome
•Shaman•N•Training•
Male User Gallery


Registered: 07/10/15
Posts: 517
Loc: United States Flag
Last seen: 7 years, 1 month
Re: Safe and Sterile inoculation Methods [Re: GoblinKing]
    #22386543 - 10/16/15 12:05 AM (8 years, 3 months ago)

SAB has never failed me...I've failed me :wow: but my SAB is the best investment I ever made. It doesn't have to be super complicated, I just cut arm holes in a tote with a dremel tool, wear gloves, tyvvek sleeves, and spray a soapy water mist before beginning work.:shrug:
Get into Agar as soon as possible, you won't regret it.:yoda:


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“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Edited by Darkhome (10/16/15 12:09 AM)


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Offlinemushpunx
Fungus Punk
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Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
Trusted Cultivator
Re: Safe and Sterile inoculation Methods [Re: Darkhome] * 1
    #22387074 - 10/16/15 05:33 AM (8 years, 3 months ago)

I agree with these guys. Flow hood or still air box. For home cultivation still air box is fine. But flow hood is sooooooooo nice to work in front of.
I agree with Mad Season , you're going to want both. There are situations I still use SAB over FH.
You need to use awesome sterile tech with both

All innoculations should start with agar regardless.


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Amateur Mycologists United
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