Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: Kalistis]
    #22387548 - 10/16/15 09:18 AM (8 years, 3 months ago)

Look for a manual online - nothing... I guess the water didnt evaporate fully, but when the jars broke and the grain spilled, it consumed a lot of the water. Thats possible right?


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: Jaboka]
    #22388880 - 10/16/15 03:16 PM (8 years, 3 months ago)

I should inject the spores trough the poly fil right, and leave it like that.


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22388922 - 10/16/15 03:29 PM (8 years, 3 months ago)

The foil on top of your jars is there to keep water from the lid dripping into your jars throwing off the water content... so you definitely don't want to throw water on top of them on purpose... when I did pf tek I used my steamer pot with a row of lid rings under the rack to keep my jars completely out of the water... bought a pc and never looked back.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22388935 - 10/16/15 03:31 PM (8 years, 3 months ago)

Quote:

Jaboka said:
Look for a manual online - nothing... I guess the water didnt evaporate fully, but when the jars broke and the grain spilled, it consumed a lot of the water. Thats possible right?



It is possible, and probably more than likely, run a test run on your pc and see if it leaks...if it did run dry it will probably be warped and not seal.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22388941 - 10/16/15 03:33 PM (8 years, 3 months ago)

Quote:

Jaboka said:
I should inject the spores trough the poly fil right, and leave it like that.



The only problem with that is when you flame your needle then stab the poly it melts and sticks to the poly...just inoculate in your sab, Crack the lid and inoculate like that.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: LocN9ne]
    #22388983 - 10/16/15 03:42 PM (8 years, 3 months ago)

OK thanks a lot. I will do that and let you know what happened. (I hope the jars are not fucked up again...)


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22389093 - 10/16/15 04:04 PM (8 years, 3 months ago)

If you doubt your jars are 100% good to go...start over...if you followed the directions properly then proceed with confidence.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: LocN9ne]
    #22389176 - 10/16/15 04:19 PM (8 years, 3 months ago)

I thinkg I did well. My only concern is the PC. I did pretty well with cleaning the room where I'll inoculate. Its in a bathroom in the shower cabin with e still air box. I cleaned so much, I can eat from the floor. :laugh:


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22389197 - 10/16/15 04:22 PM (8 years, 3 months ago)

Well that's good practice for cleaning your house... but it's really overkill for a SAB, spray the inside with soapy water, have a wet towel on the bottom and be in a room without a draft...sanitize your hands and arms and go to town...you don't need to be in the shower... but hell, high five on the extra precautionary measures.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: LocN9ne]
    #22389206 - 10/16/15 04:23 PM (8 years, 3 months ago)

What is your concern with the PC cycle now...like what makes you unsure about it?


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: LocN9ne]
    #22389299 - 10/16/15 04:44 PM (8 years, 3 months ago)

About the PC it didnt go as some people told me. It didnt whistle couple of time in a minute. It was constantly just making that pressure noise. I haven't opened the PC yet, I fear that the jars might be broken again... Fingers crossed.


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: Jaboka]
    #22389560 - 10/16/15 05:39 PM (8 years, 3 months ago)

MY SPORES DO NOT REACT ON SHAKING!!!! THEY STAY IN 1 PIECE (THE BLACK THING). i SHAKE FOR 10MIN NOW .........


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22389586 - 10/16/15 05:44 PM (8 years, 3 months ago)

Fuck that black mass... there are BILLIONS of spores in that bad boy...shake, flame sterilize, and go...you're good.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: LocN9ne]
    #22389704 - 10/16/15 06:07 PM (8 years, 3 months ago)

I inoculated. Jars are still at the still air box. Is there a risk of contamination now? And what should I do? Where should I move them?


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22389732 - 10/16/15 06:13 PM (8 years, 3 months ago)

You can leave them in your sab, or put them on a shelf somewhere that gets regular ambient light... anywhere really as long as it's not in a fuckin incubator in the dark.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: LocN9ne]
    #22389746 - 10/16/15 06:17 PM (8 years, 3 months ago)

temperature 75F right?


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22389757 - 10/16/15 06:20 PM (8 years, 3 months ago)

Room temp... 75 is optimal, but don't start over thinking this part... if it's not continously freezing, or scorching hot they are fine... that's why you need to get into agar, keep you busy...this hobby is stressful if you don't have multiple projects going... AGAR FTW.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflineJaboka
Kos... or some say Kosm?
 User Gallery


Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
Re: Inoculation question [Re: LocN9ne]
    #22389767 - 10/16/15 06:22 PM (8 years, 3 months ago)

Here it comes -----> WHAT IS AGAR?! Don't be surprised, this is my small Eastern European dictionary of urban abbreviations.


--------------------
Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Inoculation question [Re: Jaboka]
    #22389779 - 10/16/15 06:26 PM (8 years, 3 months ago)

That's not an abbreviation... it is called agar agar... it is a gelatin that we mix nutrients into, pressure cook it, let it cool and set up in petri dishes or pp5 (plastic that has the "5" recycle code on it) dishes...then drop one drop of spore solution on it in your sab, let it grow out, take a scalpel and transfer wedges of mycelium to another agar plate... used to clean up cultures... (I never go spore syringe to grains, too big of a chance of unclean spawn)it is mandatory for great success in this mycology shit.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
InvisibleKalistis
 User Gallery

Registered: 09/06/15
Posts: 2,265
Re: Inoculation question [Re: LocN9ne]
    #22390202 - 10/16/15 07:57 PM (8 years, 3 months ago)

You have the patience of a saint, Loc... You must be in an especially helpful mood.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Inoculation question montana 1,349 4 12/16/01 04:19 PM
by Karen
* Steaming and Inoculation questions podman19 1,448 7 03/29/02 12:09 AM
by WakingUpLate
* Inoculation question jackstraw 727 4 01/17/03 07:26 AM
by jackstraw
* inoculation question Snoop 1,272 4 07/26/02 05:18 AM
by FanaTEK
* inoculation question podman19 937 1 04/21/02 07:25 PM
by Mushroom_Madness
* Inoculation Question dentedshrooms 636 7 12/29/02 03:47 PM
by SomeMycologist
* mycelium inoculation question HongKongChewy 1,070 3 08/09/01 02:33 PM
by thegoldenteacher
* Question on sizes of jars... chadd 1,198 10 01/03/02 08:10 PM
by MickyFinn

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,017 topic views. 23 members, 242 guests and 61 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.01 seconds on 15 queries.