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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Kalistis]
#22385207 - 10/15/15 07:39 PM (8 years, 3 months ago) |
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Thanks for the help guys. I will take the risk got nothing to lose. The bathroom is clean. The shower cabin where I will do the magic was bleached and poured with boiling water x2. No air flow. Finishing the still air box, and getting inside. I will open the PC when I am in there, so the risk of contam is low low. I use surgeon clothes and mas and hat, sanitized twice. Gloves, mask, goggles. I think it should go smoothly and successfully.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 14 hours, 51 minutes
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Re: Inoculation question [Re: Jaboka]
#22385214 - 10/15/15 07:40 PM (8 years, 3 months ago) |
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Jaboka]
#22385216 - 10/15/15 07:41 PM (8 years, 3 months ago) |
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After I inoculate I shake the jar good right?
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculation question [Re: Jaboka]
#22385223 - 10/15/15 07:42 PM (8 years, 3 months ago) |
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Nah. I'd wait until 20% colonisation.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Mad Season]
#22385229 - 10/15/15 07:44 PM (8 years, 3 months ago) |
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Oh ok. I thought this will spread the spores on more places and it will speed the colonization.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 14 hours, 51 minutes
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Re: Inoculation question [Re: Jaboka]
#22385237 - 10/15/15 07:45 PM (8 years, 3 months ago) |
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Quote:
Jaboka said: After I inoculate I shake the jar good right?
you can but its best to shoot spores down one of the sides of the jar
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Kalistis


Registered: 09/06/15
Posts: 2,265
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When using MS you shouldn't shake after inoculation until 20 to 30% colonization. It's helpful to shoot the solution along the side of the glass so you can see the mycelium form and watch for contaminants. If you end up inoculating the middle of the grain you won't have an idea if the jar is colonizing until the growth is pretty far along because it will work its way from the inside out.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Yeah, thats what Im thinking of. 1 drop on two sides. I use 12oz jars, pretty small.. but my PC is small and i cant PCing all day...
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Jaboka]
#22385422 - 10/15/15 08:25 PM (8 years, 3 months ago) |
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B+ or golden teacher?
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Inoculation question [Re: Jaboka]
#22385447 - 10/15/15 08:29 PM (8 years, 3 months ago) |
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I'm doing both right now! That and Argentina on agar and Malabar on agar and MS to oats.
They all seem kinda the same to me. By day 4 each time I have had growth in my jars and agar after inoculation.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Kalistis]
#22385460 - 10/15/15 08:31 PM (8 years, 3 months ago) |
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I think as a 1st time ti start with B+.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Inoculation question [Re: Jaboka]
#22385462 - 10/15/15 08:31 PM (8 years, 3 months ago) |
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OK!
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Kalistis]
#22385959 - 10/15/15 10:06 PM (8 years, 3 months ago) |
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What a fail ladies and gentleman. I prepared 1:30min for this shit, I opened the PC and saw half of my jars broken, the rest still at 1 piece with burned rye at the bottom.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculation question [Re: Jaboka]
#22385996 - 10/15/15 10:13 PM (8 years, 3 months ago) |
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Was there any water left? And did you have your jars raised up out of contact with the bottom?
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Q&A US vs. THEM The more I learn, the less I know.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: LocN9ne]
#22386052 - 10/15/15 10:21 PM (8 years, 3 months ago) |
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There was no water left, but it might have been consumed by the rye. And no... I didn't put anything at the bottom. That thought was bugging me the whole time, but I didn't put anything after all. Oh well... Tomorrow.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculation question [Re: Jaboka]
#22386064 - 10/15/15 10:24 PM (8 years, 3 months ago) |
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Fuck yeah... now you got all this info and you can CONFIDENTLY bust off another batch of jars all proper...
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Q&A US vs. THEM The more I learn, the less I know.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculation question [Re: Jaboka]
#22386084 - 10/15/15 10:27 PM (8 years, 3 months ago) |
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Wow that's rough. I hope you didn't fuck up the PC. I fucked 2 PCs now getting shit to run dry.
Look at me fail!  
I'd work on not letting the weight jiggle. Sometimes you gotta tape some coins or something onto the weight, and you can get it to hover at any pressure if you adjust the burner accordingly.
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Inoculation question [Re: Jaboka]
#22386683 - 10/16/15 12:51 AM (8 years, 3 months ago) |
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Quote:
Jaboka said: There was no water left, but it might have been consumed by the rye. And no... I didn't put anything at the bottom. That thought was bugging me the whole time, but I didn't put anything after all. Oh well... Tomorrow.
If your PC came with a trivet, use it, if not... Make a layer of jar rings on the bottom of your PC, put a layer of foil over the rings. That makes a little platform so your jars don't touch the bottom. Water is VERY Important, read your manual that came with the PC...mine tells the minimum amount of water for a cycle. Some PC's have a line to mark it for you. Don't be discouraged...I've learned more from my failures than successes. Good Vibes! and...
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Darkhome]
#22387318 - 10/16/15 07:45 AM (8 years, 3 months ago) |
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Is it normal when the water starts to boil, to throw some on thentop of the jars. I had thr fear not to put too much water, i was thinking that this might flood the jars somehow
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Inoculation question [Re: Jaboka]
#22387535 - 10/16/15 09:10 AM (8 years, 3 months ago) |
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I followed the user manual al to my PC regarding how much water should be added, which ended up being 3 quarts of water. Maybe you can look you PC up online and find its user manual so you know how much water to add?
I'd say my jars are a 1/3 of the way submerged when it's full. Don't worry, you'll always be learning something new! I just figured out this week that I could stack my jars in the PC. I thought they had to be submerged in water to safety PC. I don't want to talk about how many times I have ran my PC half full. Talk about inefficient.
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