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OfflineJaboka
Kos... or some say Kosm?
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Registered: 10/28/14
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Re: Inoculation question [Re: Kalistis]
    #22385207 - 10/15/15 07:39 PM (8 years, 3 months ago)

Thanks for the help guys. I will take the risk got nothing to lose. The bathroom is clean. The shower cabin where I will do the magic was bleached and poured with boiling water x2. No air flow. Finishing the still air box, and getting inside. I will open the PC when I am in there, so the risk of contam is low low. I use surgeon clothes and mas and hat, sanitized twice. Gloves, mask, goggles. I think it should go smoothly and successfully.


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Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


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Offlinetripdawg420
low life with no life
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Re: Inoculation question [Re: Jaboka]
    #22385214 - 10/15/15 07:40 PM (8 years, 3 months ago)

:thumbup:


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HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineJaboka
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Re: Inoculation question [Re: Jaboka]
    #22385216 - 10/15/15 07:41 PM (8 years, 3 months ago)

After I inoculate I shake the jar good right?


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InvisibleMad Season
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Re: Inoculation question [Re: Jaboka]
    #22385223 - 10/15/15 07:42 PM (8 years, 3 months ago)

Nah. I'd wait until 20% colonisation.


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OfflineJaboka
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Re: Inoculation question [Re: Mad Season]
    #22385229 - 10/15/15 07:44 PM (8 years, 3 months ago)

Oh ok. I thought this will spread the spores on more places and it will speed the colonization.


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Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


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Offlinetripdawg420
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Re: Inoculation question [Re: Jaboka]
    #22385237 - 10/15/15 07:45 PM (8 years, 3 months ago)

Quote:

Jaboka said:
After I inoculate I shake the jar good right?



you can but its best to shoot spores down one of the sides of the jar :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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InvisibleKalistis
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Re: Inoculation question [Re: tripdawg420]
    #22385288 - 10/15/15 07:56 PM (8 years, 3 months ago)

When using MS you shouldn't shake after inoculation until 20 to 30% colonization. It's helpful to shoot the solution along the side of the glass so you can see the mycelium form and watch for contaminants. If you end up inoculating the middle of the grain you won't have an idea if the jar is colonizing until the growth is pretty far along because it will work its way from the inside out.


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OfflineJaboka
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Re: Inoculation question [Re: tripdawg420]
    #22385304 - 10/15/15 07:59 PM (8 years, 3 months ago)

Yeah, thats what Im thinking of. 1 drop  on two sides. I use 12oz jars, pretty small.. but my PC is small and i cant PCing all day...


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Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


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OfflineJaboka
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Re: Inoculation question [Re: Jaboka]
    #22385422 - 10/15/15 08:25 PM (8 years, 3 months ago)

B+ or golden teacher?


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Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


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InvisibleKalistis
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Re: Inoculation question [Re: Jaboka]
    #22385447 - 10/15/15 08:29 PM (8 years, 3 months ago)

I'm doing both right now! That and Argentina on agar and Malabar on agar and MS to oats.

They all seem kinda the same to me. By day 4 each time I have had growth in my jars and agar after inoculation.


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OfflineJaboka
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Re: Inoculation question [Re: Kalistis]
    #22385460 - 10/15/15 08:31 PM (8 years, 3 months ago)

I think as a 1st time ti start with B+.


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InvisibleKalistis
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Re: Inoculation question [Re: Jaboka]
    #22385462 - 10/15/15 08:31 PM (8 years, 3 months ago)

OK!


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OfflineJaboka
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Re: Inoculation question [Re: Kalistis]
    #22385959 - 10/15/15 10:06 PM (8 years, 3 months ago)

What a fail ladies and gentleman. I prepared 1:30min for this shit, I opened the PC and saw half of my jars broken, the rest still at 1 piece with burned rye at the bottom.  :rockon:


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Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


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InvisibleLocN9ne
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Re: Inoculation question [Re: Jaboka]
    #22385996 - 10/15/15 10:13 PM (8 years, 3 months ago)

Was there any water left? And did you have your jars raised up out of contact with the bottom?


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OfflineJaboka
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Re: Inoculation question [Re: LocN9ne]
    #22386052 - 10/15/15 10:21 PM (8 years, 3 months ago)

There was no water left, but it might have been consumed by the rye. And no... I didn't put anything at the bottom. That thought was bugging me the whole time, but I didn't put anything after all. Oh well... Tomorrow.


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InvisibleLocN9ne
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Re: Inoculation question [Re: Jaboka]
    #22386064 - 10/15/15 10:24 PM (8 years, 3 months ago)

Fuck yeah... now you got all this info and you can CONFIDENTLY bust off another batch of jars all proper...:vibin:


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InvisibleMad Season
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Re: Inoculation question [Re: Jaboka]
    #22386084 - 10/15/15 10:27 PM (8 years, 3 months ago)

Wow that's rough. I hope you didn't fuck up the PC. I fucked 2 PCs now getting shit to run dry.

Look at me fail! :lol:

I'd work on not letting the weight jiggle. Sometimes you gotta tape some coins or something onto the weight, and you can get it to hover at any pressure if you adjust the burner accordingly.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineDarkhome
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Registered: 07/10/15
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Re: Inoculation question [Re: Jaboka]
    #22386683 - 10/16/15 12:51 AM (8 years, 3 months ago)

Quote:

Jaboka said:
There was no water left, but it might have been consumed by the rye. And no... I didn't put anything at the bottom. That thought was bugging me the whole time, but I didn't put anything after all. Oh well... Tomorrow.



If your PC came with a trivet, use it, if not... Make a layer of jar rings on the bottom of your PC, put a layer of foil over the rings. That makes a little platform so your jars don't touch the bottom. Water is VERY Important, read your manual that came with the PC...mine tells the minimum amount of water for a cycle. Some PC's have a line to mark it for you.
Don't be discouraged...I've learned more from my failures than successes.
Good Vibes! and... :goodluck:


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“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


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OfflineJaboka
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Re: Inoculation question [Re: Darkhome]
    #22387318 - 10/16/15 07:45 AM (8 years, 3 months ago)

Is it normal when the water starts to boil, to throw some on thentop of the jars. I had thr fear not to put too much water, i was thinking that this might flood the jars somehow :laugh:


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InvisibleKalistis
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Re: Inoculation question [Re: Jaboka]
    #22387535 - 10/16/15 09:10 AM (8 years, 3 months ago)

I followed the user manual al to my PC regarding how much water should be added, which ended up being 3 quarts of water. Maybe you can look you PC up online and find its user manual so you know how much water to add?

I'd say my jars are a 1/3 of the way submerged when it's full. Don't worry, you'll always be learning something new! I just figured out this week that I could stack my jars in the PC. I thought they had to be submerged in water to safety PC. I don't want to talk about how many times I have ran my PC half full. Talk about inefficient.


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