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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Inoculation question
#22384770 - 10/15/15 06:04 PM (8 years, 3 months ago) |
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When sterilizing the jars, I flipped the pins upside down for the sterilization. Should I leave them like that during inoculation and not open them at all till they fully colonize, or I should put them back to normal? Im asking because I want to eliminate any chance of contamination, and opening them might raise the risk. Thanks
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculation question [Re: Jaboka]
#22384779 - 10/15/15 06:05 PM (8 years, 3 months ago) |
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You pressure cooked your brf cakes upside down?
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Q&A US vs. THEM The more I learn, the less I know.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: LocN9ne]
#22384793 - 10/15/15 06:10 PM (8 years, 3 months ago) |
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No. Just the lids are upside down, so the rubber ring faces the sealing.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculation question [Re: Jaboka]
#22384803 - 10/15/15 06:12 PM (8 years, 3 months ago) |
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Oh... leave em that way, you got inoculation holes and a dry Verm layer right?
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Q&A US vs. THEM The more I learn, the less I know.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: LocN9ne]
#22384878 - 10/15/15 06:28 PM (8 years, 3 months ago) |
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No I did rye only. http://www.shroomery.org/forums/showflat.php/Number/20048765 This is the tek I followed. So I leave them like they are, no opening.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculation question [Re: Jaboka]
#22384899 - 10/15/15 06:32 PM (8 years, 3 months ago) |
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I was way off... sorry bout that...I'd leave them.
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Q&A US vs. THEM The more I learn, the less I know.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: LocN9ne]
#22384946 - 10/15/15 06:44 PM (8 years, 3 months ago) |
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I forgot a take pics for my journal. Heres what im talking about.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculation question [Re: Jaboka]
#22384982 - 10/15/15 06:53 PM (8 years, 3 months ago) |
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Where's the gas exchange filters? Or are you going to add those soon? Or is it a dry verm layer?? So confused lol
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculation question [Re: Mad Season]
#22385016 - 10/15/15 06:59 PM (8 years, 3 months ago) |
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Is there a cfd or tyvek under those lids? If not there needs to be, or polyfil stuffed in there.
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Q&A US vs. THEM The more I learn, the less I know.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Mad Season]
#22385020 - 10/15/15 07:00 PM (8 years, 3 months ago) |
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I am more confused than you bro. Im virging on these things. I follow the tek i posted above and thats it. No vermiculate not shit. Just rye. i didnt have the sdf so i didnt use one. I will seal the holes with poly fill after inoculation. If you have any advice give it here.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculation question [Re: Jaboka]
#22385034 - 10/15/15 07:03 PM (8 years, 3 months ago) |
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My advice is to put the poly in before the PC cycle. This will sterilize the filter, and keep it sterile until you inoculate. Do you have a still air box? If you do you can just pop the lid, tilt the jar and drop 3 drops of solution down 1 side. Then put the lid back on .
Edited by Mad Season (10/15/15 07:04 PM)
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Inoculation question [Re: Jaboka]
#22385046 - 10/15/15 07:06 PM (8 years, 3 months ago) |
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Well having open holes in your lids after pc'ing is all bad, they were sterilized but I doubt it now... make up another batch after you get poly stuffed in those holes...pc them with the poly stuffed lids covered in foil.
What mad said.
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Q&A US vs. THEM The more I learn, the less I know.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Mad Season]
#22385065 - 10/15/15 07:10 PM (8 years, 3 months ago) |
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Oh i thought i need the poly after inoculations. I am working in the still box right now. If you have a link for a good still air box give it here pls
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Jaboka]
#22385079 - 10/15/15 07:12 PM (8 years, 3 months ago) |
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The jars are still at the PC. I havent opened anything yet. I was sanitizing a bathroom and the shower cabin (Ill inoculste inside the cabin). I will open the PC when im in there with still air box.
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculation question [Re: Jaboka]
#22385081 - 10/15/15 07:13 PM (8 years, 3 months ago) |
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Quote:
Making the still air box (SAB)
This is a picture of my 110 qt clear still air box:
 Notice it's wet/condensated inside. That's what it's supposed to look like the whole time you're working with it.
I made it mimicking this video, except I SERIOUSLY recommend you get a 100 qt+ tub.
I took the cut outs of the holes and taped them to make little doors. This is to keep the air still if I'm waiting for things to cool, or if I want a smoke break.
My SAB holes are 6 inches. Honestly this is the MINIMUM diameter. I took incense and lit it in my box (kudos to kizzle for the idea) and found the arm holes moved the most air (smoke) when moving my arms around, and in and out. By increasing the size of the arm holes, and working in a fairly still room with the vents turned off .. I've been finding that moving your arms in and out will cause no air movement. And thus very rare contams.
http://www.shroomery.org/forums/showflat.php/Number/21889950
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Jaboka
Kos... or some say Kosm?



Registered: 10/28/14
Posts: 327
Loc: IC 1101
Last seen: 6 months, 18 days
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Re: Inoculation question [Re: Jaboka]
#22385089 - 10/15/15 07:14 PM (8 years, 3 months ago) |
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Does someone have pics of how the lids with poly should look like?
-------------------- Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculation question [Re: Jaboka]
#22385097 - 10/15/15 07:18 PM (8 years, 3 months ago) |
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I use poly on my pasty plates.. They're in that link.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 13 hours, 26 minutes
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Re: Inoculation question [Re: Jaboka]
#22385100 - 10/15/15 07:19 PM (8 years, 3 months ago) |
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Quote:
Jaboka said: Does someone have pics of how the lids with poly should look like?
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Kalistis


Registered: 09/06/15
Posts: 2,265
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I used synthetic filter discs instead of polyfil and have made a couple different types of lids. Some with silicone injection ports, some without. No issues with contaminants yet with either.
No port:

Port:

Really easy to make, but you do have to plan ahead before PCing your grain.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 13 hours, 26 minutes
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Quote:
tripdawg420 said:
Quote:
Jaboka said: Does someone have pics of how the lids with poly should look like?

heres a lil better pic of the tops
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