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OfflineJaboka
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Inoculation question
    #22384770 - 10/15/15 06:04 PM (8 years, 3 months ago)

When  sterilizing the jars, I flipped the pins upside down for the sterilization. Should I leave them like that during inoculation and not open them at all till they fully colonize, or I should put them back to normal? Im asking because I want to eliminate any chance of contamination, and opening them might raise the risk.
Thanks


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InvisibleLocN9ne
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Re: Inoculation question [Re: Jaboka]
    #22384779 - 10/15/15 06:05 PM (8 years, 3 months ago)

You pressure cooked your brf cakes upside down?


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OfflineJaboka
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Re: Inoculation question [Re: LocN9ne]
    #22384793 - 10/15/15 06:10 PM (8 years, 3 months ago)

No. Just the lids are upside down, so the rubber ring faces the sealing.


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InvisibleLocN9ne
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Re: Inoculation question [Re: Jaboka]
    #22384803 - 10/15/15 06:12 PM (8 years, 3 months ago)

Oh... leave em that way, you got inoculation holes and a dry Verm layer right?


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OfflineJaboka
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Re: Inoculation question [Re: LocN9ne]
    #22384878 - 10/15/15 06:28 PM (8 years, 3 months ago)

No I did rye only. http://www.shroomery.org/forums/showflat.php/Number/20048765 ; This is the tek I followed. So I leave them like they are, no opening.


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InvisibleLocN9ne
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Re: Inoculation question [Re: Jaboka]
    #22384899 - 10/15/15 06:32 PM (8 years, 3 months ago)

I was way off... sorry bout that...I'd leave them.


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OfflineJaboka
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Re: Inoculation question [Re: LocN9ne]
    #22384946 - 10/15/15 06:44 PM (8 years, 3 months ago)



I forgot a take pics for my journal. Heres what im talking about.


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InvisibleMad Season
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Re: Inoculation question [Re: Jaboka]
    #22384982 - 10/15/15 06:53 PM (8 years, 3 months ago)

Where's the gas exchange filters? Or are you going to add those soon? Or is it a dry verm layer?? So confused lol


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InvisibleLocN9ne
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Re: Inoculation question [Re: Mad Season]
    #22385016 - 10/15/15 06:59 PM (8 years, 3 months ago)

Is there a cfd or tyvek under those lids? If not there needs to be, or polyfil stuffed in there.


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OfflineJaboka
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Re: Inoculation question [Re: Mad Season]
    #22385020 - 10/15/15 07:00 PM (8 years, 3 months ago)

I am more confused than you bro. Im virging on these things. I follow the tek i posted above and thats it. No vermiculate not shit. Just rye. i didnt have the sdf so i didnt use one. I will seal the holes with poly fill after inoculation. If you have any advice give it here.


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InvisibleMad Season
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Re: Inoculation question [Re: Jaboka]
    #22385034 - 10/15/15 07:03 PM (8 years, 3 months ago)

My advice is to put the poly in before the PC cycle. This will sterilize the filter, and keep it sterile until you inoculate. Do you have a still air box? If you do you can just pop the lid, tilt the jar and drop 3 drops of solution down 1 side. Then put the lid back on :smile:.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Edited by Mad Season (10/15/15 07:04 PM)


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InvisibleLocN9ne
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Re: Inoculation question [Re: Jaboka]
    #22385046 - 10/15/15 07:06 PM (8 years, 3 months ago)

Well having open holes in your lids after pc'ing is all bad, they were sterilized but I doubt it now... make up another batch after you get poly stuffed in those holes...pc them with the poly stuffed lids covered in foil.

What mad said.


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OfflineJaboka
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Re: Inoculation question [Re: Mad Season]
    #22385065 - 10/15/15 07:10 PM (8 years, 3 months ago)

Oh i thought i need the poly after inoculations. I am working in the still box right now. If you have a link for a good still air box give it here pls


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OfflineJaboka
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Re: Inoculation question [Re: Jaboka]
    #22385079 - 10/15/15 07:12 PM (8 years, 3 months ago)

The jars are still at the PC. I havent opened anything yet. I was sanitizing a bathroom and the shower cabin (Ill inoculste inside the cabin). I will open the PC when im in there with still air box.


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InvisibleMad Season
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Re: Inoculation question [Re: Jaboka]
    #22385081 - 10/15/15 07:13 PM (8 years, 3 months ago)

Quote:

Making the still air box (SAB)

This is a picture of my 110 qt clear still air box:

Notice it's wet/condensated inside. That's what it's supposed to look like the whole time you're working with it.

I made it mimicking this video, except I SERIOUSLY recommend you get a 100 qt+ tub.



I took the cut outs of the holes and taped them to make little doors. This is to keep the air still if I'm waiting for things to cool, or if I want a smoke break.

My SAB holes are 6 inches. Honestly this is the MINIMUM diameter. I took incense and lit it in my box (kudos to kizzle for the idea) and found the arm holes moved the most air (smoke) when moving my arms around, and in and out. By increasing the size of the arm holes, and working in a fairly still room with the vents turned off .. I've been finding that moving your arms in and out will cause no air movement. And thus very rare contams.




http://www.shroomery.org/forums/showflat.php/Number/21889950


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineJaboka
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Re: Inoculation question [Re: Jaboka]
    #22385089 - 10/15/15 07:14 PM (8 years, 3 months ago)

Does someone have pics of how the lids with poly should look like?


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InvisibleMad Season
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Re: Inoculation question [Re: Jaboka]
    #22385097 - 10/15/15 07:18 PM (8 years, 3 months ago)

I use poly on my pasty plates.. They're in that link.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinetripdawg420
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Re: Inoculation question [Re: Jaboka]
    #22385100 - 10/15/15 07:19 PM (8 years, 3 months ago)

Quote:

Jaboka said:
Does someone have pics of how the lids with poly should look like?





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HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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InvisibleKalistis
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Re: Inoculation question [Re: tripdawg420]
    #22385179 - 10/15/15 07:32 PM (8 years, 3 months ago)

I used synthetic filter discs instead of polyfil and have made a couple different types of lids. Some with silicone injection ports, some without. No issues with contaminants yet with either.

No port:



Port:



Really easy to make, but you do have to plan ahead before PCing your grain.  :shrug:


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Offlinetripdawg420
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Re: Inoculation question [Re: tripdawg420]
    #22385196 - 10/15/15 07:37 PM (8 years, 3 months ago)

Quote:

tripdawg420 said:
Quote:

Jaboka said:
Does someone have pics of how the lids with poly should look like?







heres a lil better pic of the tops


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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