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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineRaxV
Stranger
Registered: 10/15/15
Posts: 1
Last seen: 8 years, 3 months
Possible alternate grain sterilization method with dry heat. Thoughts?
    #22383674 - 10/15/15 02:14 PM (8 years, 3 months ago)

I have been researching methods for sterilizing grain for spawn in larger quantities than canning jars.  Because of the endospores I have found limited alternate methods to pressure cooker, but have come up with one idea from my research and wondered if anyone has tried/succeeded/failed with anything similar.
Endospores can be killed in dry heat at >350F for more than 2 hours.  My thought is to bake my grain in the oven at 400F for 2+ hours after the grain comes up to temperature.  This is before I hydrate the grain since all moisture will need to cook off before the internal temperature of the grain can get above boiling point of water.
After the baking sterilization I would hydrate the grain in bleach water to prevent re-contamination.  After hydration I would cook the grain in the same water to drive off the chlorine as gas. 
Cover tightly and let cool.  Inoculate immediately after cool and transfer to bags in as clean environment as possible.  I'm building clean box for transfer and culture use.
Has anyone try this or have thoughts on things I might have missed?


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Invisiblesweetpea
Female
Registered: 12/29/13
Posts: 327
. [Re: RaxV]
    #22383725 - 10/15/15 02:28 PM (8 years, 3 months ago)

.


Edited by sweetpea (10/17/15 11:37 PM)


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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