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Invisiblesimpleshroomer6283
shroomer
I'm a teapot

Registered: 10/15/14
Posts: 151
Loc: Land of colorful things
Old print contamination
    #22383616 - 10/15/15 02:01 PM (8 years, 3 months ago)

Hey guys,

Ive already grown mushies successfully twice and I havent grown any since february and I made a print in February but I didnt take big precautions so I wanted to know if its still possible to make a contamination free cake? :smile:

Thanks for answers in advance


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InvisibleGoblinKing
Trismegistus

Registered: 08/17/15
Posts: 328
Loc: Right where it belongs.
Re: Old print contamination [Re: simpleshroomer6283]
    #22385743 - 10/15/15 09:20 PM (8 years, 3 months ago)



I can only guess. You should go for it. What's the worst that could happen?


--------------------


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Old print contamination [Re: GoblinKing]
    #22385850 - 10/15/15 09:45 PM (8 years, 3 months ago)

If you want a truly contam free cake, then take the extra step, and swipe the print (with a sterilized Q-tip) on some agar plates.

Wait for clean growth and make a LI syringe to inoculate your brf jars with.

Growth will be a LOT faster, and the best part...no contams to worry about (given you perform proper sterile techniques while working in your SAB).

Here's my favorite LI tek: http://www.shroomery.org/forums/showflat.php/Number/20429745

And also a tek for practicing your sterile techniques! http://www.shroomery.org/forums/showflat.php/Number/21889950/fpart/1/vc/1 :super:


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Invisiblesimpleshroomer6283
shroomer
I'm a teapot

Registered: 10/15/14
Posts: 151
Loc: Land of colorful things
Re: Old print contamination [Re: GoblinKing]
    #22396349 - 10/18/15 05:32 AM (8 years, 3 months ago)

Quote:

Psilosoulful said:
If you want a truly contam free cake, then take the extra step, and swipe the print (with a sterilized Q-tip) on some agar plates.

Wait for clean growth and make a LI syringe to inoculate your brf jars with.

Growth will be a LOT faster, and the best part...no contams to worry about (given you perform proper sterile techniques while working in your SAB).

Here's my favorite LI tek: http://www.shroomery.org/forums/showflat.php/Number/20429745

And also a tek for practicing your sterile techniques! http://www.shroomery.org/forums/showflat.php/Number/21889950/fpart/1/vc/1 :super:




Yeah cant find agar anywhere here Im probably going to use the honey tek for this. Thanks for the input :smile:


Quote:

GoblinKing said:


I can only guess. You should go for it. What's the worst that could happen?




I will just go for it :laugh:


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InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Old print contamination [Re: simpleshroomer6283]
    #22396741 - 10/18/15 09:06 AM (8 years, 3 months ago)

Using spores in a sugar LC is by far riskier than using those same spores to inoculate cakes. LCs in general favor contams which is why people recommend agar wedges to inoculate them.


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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
Trusted Cultivator
Re: Old print contamination [Re: Pastywhyte]
    #22396830 - 10/18/15 09:38 AM (8 years, 3 months ago)

Quote:

Pastywhyte said:
Using spores in a sugar LC is by far riskier than using those same spores to inoculate cakes.



+1, especially as he made the print himself and says he did not precautions.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Old print contamination [Re: blackout]
    #22396900 - 10/18/15 10:05 AM (8 years, 3 months ago)

I would keep a print you're unsure of away from anything but agar.
If you want to make an excuse to why agar isnt something you can do right now the PF cake is the only other thing I would try questionable spores on.


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OfflineRJ Tubs 202
Male


Registered: 09/20/08
Posts: 6,016
Loc: USA Flag
Last seen: 7 hours, 55 minutes
Re: Old print contamination [Re: Pastywhyte]
    #22397067 - 10/18/15 10:51 AM (8 years, 3 months ago)

Quote:

Pastywhyte said:
Using spores in a sugar LC is by far riskier than using those same spores to inoculate cakes. LCs in general favor contams which is why people recommend agar wedges to inoculate them.




To be clear, is one good method to put a tiny bit of spores (knife tip), or a drop from a syringe, in the middle of the agar plate, and then wait until growth is about quarter size, and then transfer from the very outer edge of the circle to a new plate, and then use that to inoc? Just want to be clear on the best way to do this...        thanks


Edited by RJ Tubs 202 (10/18/15 10:52 AM)


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Invisiblemicelio
Song of Silence
 User Gallery

Registered: 04/22/14
Posts: 1,547
Re: Old print contamination [Re: simpleshroomer6283]
    #22397141 - 10/18/15 11:13 AM (8 years, 3 months ago)

Quote:

Medocore said:
Quote:

Psilosoulful said:
If you want a truly contam free cake, then take the extra step, and swipe the print (with a sterilized Q-tip) on some agar plates.

Wait for clean growth and make a LI syringe to inoculate your brf jars with.

Growth will be a LOT faster, and the best part...no contams to worry about (given you perform proper sterile techniques while working in your SAB).

Here's my favorite LI tek: http://www.shroomery.org/forums/showflat.php/Number/20429745

And also a tek for practicing your sterile techniques! http://www.shroomery.org/forums/showflat.php/Number/21889950/fpart/1/vc/1 :super:




Yeah cant find agar anywhere here Im probably going to use the honey tek for this. Thanks for the input :smile:


Quote:

GoblinKing said:


I can only guess. You should go for it. What's the worst that could happen?




I will just go for it :laugh:




In a pinch you could use Agar substitute..

http://www.shroomery.org/8514/Agar-substitute

I have had good success using this method.. But does not compare to the real thing (agar)...


--------------------
     


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InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Old print contamination [Re: RJ Tubs 202]
    #22397177 - 10/18/15 11:24 AM (8 years, 3 months ago)

Quote:

RJ Tubs 202 said:
Quote:

Pastywhyte said:
Using spores in a sugar LC is by far riskier than using those same spores to inoculate cakes. LCs in general favor contams which is why people recommend agar wedges to inoculate them.




To be clear, is one good method to put a tiny bit of spores (knife tip), or a drop from a syringe, in the middle of the agar plate, and then wait until growth is about quarter size, and then transfer from the very outer edge of the circle to a new plate, and then use that to inoc? Just want to be clear on the best way to do this...        thanks




Pretty much all there is too it. Also agar is not hard to find unless you live in the arctic circle or something. Very easy to buy online as well. Asian food shops, health food stores will both carry it. A lb will last the average hobbiest years.


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