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Offlineuninc4life2010
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How many transfers should I perform on agar, and how large of a wedge should I use to inoculate?
    #22382306 - 10/15/15 07:51 AM (8 years, 3 months ago)

I don't really feel the need to post any pics, but I put ATL#7 spores on two agar plates, and one had shown signs of growth after 3 weeks.  I saw what looked like bactria colonies, so I took two samples of healthy mycelium from the side opposite the contams and transferred to two new plates.  Is it necessary to perform a second transfer with each of the plates?  How many transfers are necessary before a consistent strain is isolated?  Also, how large of a wedge should I inoculate my quart sized oat jars with?  I was planning on allowing the mycelium to grow out to roughly 2/3 the diameter of the plate, then use about 1/8th of what is there.  Kinda like a pizza slice wedge.


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Invisibleblindingleaf
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Re: How many transfers should I perform on agar, and how large of a wedge should I use to inoculate? [Re: uninc4life2010]
    #22382412 - 10/15/15 08:31 AM (8 years, 3 months ago)

i use as much agar as possible to inoculate.

if u were running from bacteria, that next plate should be fine to use.
if ur running from mold, i'd say do another transfer.

if u are trying to isolate from spore, it could take many transfers.
if ur just trying to noc up some jars, no need to do that really unless u want to.
noc grain with clean MS>bulk>clone

or let plate fruit/form stones>clone from the plate.


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MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlineuninc4life2010
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Re: How many transfers should I perform on agar, and how large of a wedge should I use to inoculate? [Re: blindingleaf]
    #22382468 - 10/15/15 08:54 AM (8 years, 3 months ago)

Okay, sounds like a plan.  Hopefully I won't see any more contams on either plate.  I'm really excited for this project since it is my first time working with a sclerotia producing species.


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Invisibleblindingleaf
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Re: How many transfers should I perform on agar, and how large of a wedge should I use to inoculate? [Re: uninc4life2010]
    #22382483 - 10/15/15 08:58 AM (8 years, 3 months ago)

sweet.  sclerotia are awesome.
post a pic of new xfer plate if ur unsure.  but IME, as long as the bacteria is not intertwined with the culture, and just a separate colony on a plate, its easy to get away from.

mold is a harder problem to tackle.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinecronicrFacebook
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Re: How many transfers should I perform on agar, and how large of a wedge should I use to inoculate? [Re: blindingleaf]
    #22382843 - 10/15/15 10:50 AM (8 years, 3 months ago)

:whathesaid:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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