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Offlinethetawave
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Registered: 08/03/13
Posts: 96
Last seen: 6 months, 22 days
Is this honey LC contaminated?
    #22382027 - 10/15/15 05:50 AM (8 years, 3 months ago)

Hi guys.

Can an experienced grower advise me to what is happening here

Looks like we have some sort of contamination but not 100% sure.

This is a water+honey LC inoculated with GT spores on Sept 30.

Is the culture supposed to be completely pure white? This is my first time making LC.

Thanks.





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InvisibleNewToCultivation
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Registered: 08/31/15
Posts: 185
Re: Is this honey LC contaminated? [Re: thetawave]
    #22382045 - 10/15/15 06:02 AM (8 years, 3 months ago)

Quote:

thetawave said:
Hi guys.

Can an experienced grower advise me to what is happening here

Looks like we have some sort of contamination but not 100% sure.

This is a water+honey LC inoculated with GT spores on Sept 30.

Is the culture supposed to be completely pure white? This is my first time making LC.

Thanks.








Yeah that's definitely fuckered, looks like it would mutate your arm if opened.


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Offlinethetawave
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Re: Is this honey LC contaminated? [Re: NewToCultivation]
    #22382119 - 10/15/15 06:35 AM (8 years, 3 months ago)

Quote:

NewToCultivation said:
Yeah that's definitely fuckered, looks like it would mutate your arm if opened.




lol yeah it does. so the mycelium should be shiny white and fluffy? I can't seem to find good pictures of healthy LC.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Is this honey LC contaminated? [Re: thetawave]
    #22382143 - 10/15/15 06:42 AM (8 years, 3 months ago)

Was this lc inoculated with a spore syringe? If so, how many cc's did you inject?


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Offlinetetherface
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Registered: 10/05/14
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Re: Is this honey LC contaminated? [Re: insanemike]
    #22382307 - 10/15/15 07:51 AM (8 years, 3 months ago)

Its prolly trash because you went spore->lc which is a very big gamble...but id test it anyways inoculate a jar or a plate and see what grows you never know maybe you got lucky:shrug:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Is this honey LC contaminated? [Re: tetherface]
    #22382328 - 10/15/15 07:56 AM (8 years, 3 months ago)

What I was getting at is that it looks like a lot of ungerminated spores in the middle of the network. It happens when you add too much inoculant from a spore syringe into an lc. It may be good but I would noc up one grain jar and if it's good then expand from there.


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Offlinethetawave
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Re: Is this honey LC contaminated? [Re: insanemike]
    #22382337 - 10/15/15 08:00 AM (8 years, 3 months ago)

Quote:

insanemike said:
Was this lc inoculated with a spore syringe? If so, how many cc's did you inject?




Actually I just dropped spores off a tinfoil print into the jar.

I threw in alot of spores..

So I guess the much safer route is going from spore directly to agar?


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Offlinetetherface
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Re: Is this honey LC contaminated? [Re: thetawave]
    #22382365 - 10/15/15 08:11 AM (8 years, 3 months ago)

The best route is always spores->agar->whatever I only make lcs from agar wedges or 9er tek macerated tissue clones


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Is this honey LC contaminated? [Re: tetherface]
    #22382372 - 10/15/15 08:15 AM (8 years, 3 months ago)

Agar FTW


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OfflineMoldy thumb
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Re: Is this honey LC contaminated? [Re: insanemike]
    #22382401 - 10/15/15 08:26 AM (8 years, 3 months ago)

Haha LC's are finicky. I've knocked email up with spires and got absolutely nothing. I've also used tissue from a clone and thought I had something really aggressive. Turns out I was growing a beautiful sample of cobweb.


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Invisibleblindingleaf
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Re: Is this honey LC contaminated? [Re: Moldy thumb]
    #22382471 - 10/15/15 08:54 AM (8 years, 3 months ago)

Quote:

Actually I just dropped spores off a tinfoil print into the jar.




thats more risky than spore syringe to LC dude!!

I agree with mike.


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InvisibleMr.GuessWork
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Re: Is this honey LC contaminated? [Re: blindingleaf]
    #22382496 - 10/15/15 09:03 AM (8 years, 3 months ago)

Spores>solid agar>whatever is definitely the best idea. You need the solid growth medium made with a streaking technique to separate the contaminants from the good stuff. If you go from dirty spores strait to a liquid medium, then you're growing a mix of contaminants and the desired mycelium, and the contams always win.


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OfflineDrCrumbs
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Re: Is this honey LC contaminated? [Re: blindingleaf]
    #22382520 - 10/15/15 09:17 AM (8 years, 3 months ago)

I've done this exact some thing before, in a cleaned and almost still room (I know not the best thing), to a bunch of LC jars.

Not all did this black thing only a couple. The ones that did grew bacteria on WBS test jars.


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Offlinethetawave
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Re: Is this honey LC contaminated? [Re: DrCrumbs]
    #22387133 - 10/16/15 06:11 AM (8 years, 3 months ago)

thank you all for the replies. I have learnt my lesson and inoculated some agar dishes with spores today :smile:


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OfflineSmoothcat
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Registered: 10/07/15
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Re: Is this honey LC contaminated? [Re: thetawave]
    #22387154 - 10/16/15 06:32 AM (8 years, 3 months ago)

Your one don't look good dude sorry :sad:

I've got this one chilling out in my fridge at the moment, it looks clean but I won't know till I actually nocc something up with it.





Hope this helps dude

Not tried agar yet, it's next on the list :cool:


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InvisibleDaveyJones6911
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Re: Is this honey LC contaminated? [Re: Smoothcat]
    #22387341 - 10/16/15 07:53 AM (8 years, 3 months ago)

uknow i did see a tek for an alternative way to start an LC among other things:

make a syringe with sterile water, take fully colonised pf jar, inject water, shake, suck up water.
you should now have a syringe with water and some myc in there. in the tek it is used to inoculate jars,
but how about using that to start an LC?

also could one use a PF cake and cut a wedge from that instead of an agar plate?


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Offlineblackout
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Re: Is this honey LC contaminated? [Re: DaveyJones6911]
    #22388514 - 10/16/15 02:02 PM (8 years, 3 months ago)

Honey has proteins which will settle at the bottom, I would not use if for LCs. As you scraped a print it is more than likely contaminated.

If you insist on using honey I would boil it first, let it settle in the fridge for a day or 2 and decant it off the sediement and sterilize the now clear liquid.

I would also use very little, aim for a concentration of about 0.4% which is 1/10th what most say. This goes for all LCs, not just honey.


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