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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Brown Rice Flour, Rye Grain, Vermiculite, Wild Bird Seed

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OfflineTheDrugStore
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Registered: 12/23/03
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Last seen: 1 year, 10 months
First timer, just innoculated...what a bitch it was
    #2238141 - 01/13/04 12:56 AM (12 years, 10 months ago)

I dont know why i had this difficulty. I would burn the end of the syringe then shake and insert it into the jar in one of the 4 holes on top. The first hole would go fine...but when i got to the second it fucking wouldn't spray shit cuz the damn verm kept getting inside. So i had to push it but push it hard...although not hard enough to dump the whole 10cc's into the jar at once. Finally the verm would come out and a WHOLE cc would be wasted. That sucked. Anyways basically this happened to all my 8 jars...and used up the 11cc's of EC i had and also had to resort to my other syringe to finish the last 2 that weren't yet innoculated. So i got out the south american spores and repeted the same method... a little trouble but it went good.Wnt over the 1CC per using 1 1/2cc's for the last 2.  :crazy: Well i guess if this shit doesn't work out well i still have 7 cc's of SA to save me.

Wish me luck. Hopefully i can correctly grow some damn shrooms  :mushroom2:

Peace all,
Drug


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Penalties against drug use should not be more damaging to an individual than the use of the drug itself. Nowhere is this more clear than in the laws against the possession of marijuana and mushrooms in private for personal use.


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InvisibleMOTH
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Registered: 06/06/03
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Re: First timer, just innoculated...what a bitch it was [Re: TheDrugStore]
    #2238238 - 01/13/04 01:47 AM (12 years, 10 months ago)

My kitten always moves the syringe into the jars with the opening in the needle pressed against the glass. Takes minimal pushing to get the spores in, and verm stays out. Also make sure that your needle is cool (my kitten wipes hers with an alcohol puff ) after sterlizing with flame. Maybe this will work with your buddy?


Edited by EllemyshShade (01/13/04 01:48 AM)


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Invisiblesimplemachine
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Registered: 09/14/03
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Re: First timer, just innoculated...what a bitch it was [Re: MOTH]
    #2238251 - 01/13/04 01:57 AM (12 years, 10 months ago)

1-2 cc is by no means too much, you have wasted nothing.

If you have jars you can shake, all you need in 1 hole 1 shot, Then shake to distribute spores.

also make sure to cool the syringe by wiping in alcohol as ellmy suggests. Thats all I do, no flame is needed.


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Invisiblesimplemachine
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Re: First timer, just innoculated...what a bitch it was [Re: simplemachine]
    #2238255 - 01/13/04 01:59 AM (12 years, 10 months ago)

I also only fill my jars 2/3 full and use no dry verm barrier, this means the needle never even touches the substrate.


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Offlineamyloid
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Re: First timer, just innoculated...what a bitch it was [Re: simplemachine]
    #2238260 - 01/13/04 02:01 AM (12 years, 10 months ago)

i dont shake after innoculating, i have better luck this way.


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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Invisiblesimplemachine
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Re: First timer, just innoculated...what a bitch it was [Re: amyloid]
    #2238264 - 01/13/04 02:05 AM (12 years, 10 months ago)

>i dont shake after innoculating, i have better luck this way.

To each his own. I just like having jars that colonize in a week instead of three or four. I envy your patience!


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Offlineamyloid
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Re: First timer, just innoculated...what a bitch it was [Re: simplemachine]
    #2238272 - 01/13/04 02:10 AM (12 years, 10 months ago)

heh, they dont take more then 2 weeks @ ~86f. i shake after i see some growth though, and again when its about 2/3rds colonized. at least that is what i was doing with rye grain, im trying millet+rape+quinoa now ill hafta see how fast this colonizes.


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Invisiblesimplemachine
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Re: First timer, just innoculated...what a bitch it was [Re: amyloid]
    #2238303 - 01/13/04 02:19 AM (12 years, 10 months ago)

yeh thats apx my method too! when I was using non shakeable cakes it took FOREVER!! but wita little experimenting and reading I got pf style cakes I can shake the crap out of! I shoot shake and incubate till they germinate. Then I shake about every other day depending on how fast colonization is. Full colonization is achived in apx 1 week with an agressive strain such as tazmanian of equadorian. Having a polyfill or other gas exchange filter on a pf jar will also GREATLY speed colonization. Grain jars are known to colonize MUCH faster than verm/flour cakes, but using my methods I get full pf cakes in about a week when incubating in the 86-90 degree range.


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InvisibleYesItsMe
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Registered: 10/29/03
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Re: First timer, just innoculated...what a bitch it was [Re: simplemachine]
    #2238441 - 01/13/04 05:39 AM (12 years, 10 months ago)

What is this shakeable pf tek ?
Verm+Water+Brf+Wbs or what ?
Interresting post , 1 week for full colonization of pf cake ... :smile:


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Invisiblesimplemachine
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Re: First timer, just innoculated...what a bitch it was [Re: YesItsMe]
    #2239720 - 01/13/04 06:08 PM (12 years, 10 months ago)

It's EASY! Just make a regular pf mix with millet flour

1 cup millet
2/3 cup water
2 cups verm

fill half pints 1/2 - 2/3 full. no dry verm barrier. 1 hole for innoculation, and 1 larger hole for polyfill filter.

just bust up the jars after sterilzing, while they are still hot whack it against the floor and break that shit back up!

Shake well after innoc and maybe every other day as the myc grows out.


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Offlinevermonster
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Registered: 10/17/03
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Re: First timer, just innoculated...what a bitch it was [Re: TheDrugStore]
    #2239781 - 01/13/04 06:32 PM (12 years, 10 months ago)

My friend uses a still air glovebox for knocking up
BRF jars and isn't too keen on a open flame in there.
She has an alcohol lamp and heats the needle once
and then sets the syringe, needle first, in a small jar
of alcohol. Puts out the lamp and then shoots the jar
and sets the syringe back in the alcohol jar between
innoculations. Did this on 1/2 to 6 jars and so far no
contams  :eyemouth:


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Invisiblesimplemachine
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Re: First timer, just innoculated...what a bitch it was [Re: vermonster]
    #2239810 - 01/13/04 06:43 PM (12 years, 10 months ago)

>My friend uses a still air glovebox for knocking up


I don't think this is needed unless your knocking your jars in a mold factory, but extra protection is always good. A glovebox is however needed for gtg transfers, cloning, or other sterile work.


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Anonymous

Re: First timer, just innoculated...what a bitch it was [Re: TheDrugStore]
    #2239827 - 01/13/04 06:50 PM (12 years, 10 months ago)

here's a tip...after each holes is done, heat the sharp to red hot and the blockage should pop out.


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OfflineOmegaRed
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Registered: 04/16/03
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Re: First timer, just innoculated...what a bitch it was [Re: TheDrugStore]
    #2240402 - 01/13/04 11:00 PM (12 years, 10 months ago)

Drug Store,

This is not a problem with verm getting into the syringe, it is a problem with how you are injecting. The pressure on the hammer (the pushy thing) must be in line with the actual syringe which holds the spores. When you push on the hammer at an angle that is not in line with the syringe, the hammer gets stuck and you will push to hard and when it breaks free it will inject much more than you intend.


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I'm not sleepy and there's no place I'm going to.
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OfflineTheDrugStore
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Registered: 12/23/03
Posts: 661
Loc: Hawaii Flag
Last seen: 1 year, 10 months
Re: First timer, just innoculated...what a bitch it was [Re: OmegaRed]
    #2240460 - 01/14/04 12:48 AM (12 years, 10 months ago)

Hmmm... that might have been the problem. I didn't notice it but i wasn't looking for a problem like that... i just thought something was in the needle glocking it. Next time i do injecting ill keep that in mind. Now that i think about it... that probly was my problem..FUCK.

Thanks man


--------------------
Penalties against drug use should not be more damaging to an individual than the use of the drug itself. Nowhere is this more clear than in the laws against the possession of marijuana and mushrooms in private for personal use.


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Anonymous

Re: First timer, just innoculated...what a bitch it was [Re: TheDrugStore]
    #2240485 - 01/14/04 12:54 AM (12 years, 10 months ago)

in my prior myco study days, i would always get verm stuck in the needle. and yes it is a bitch. a lot of the time i would end up shooting more than planned. It may have been your plunger alignment, but if you are using horticulture grade verm (the small stuff) like i did, it is your verm.


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OfflineJetBlackNinja
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Re: First timer, just innoculated...what a bitch it was [Re: simplemachine]
    #2241000 - 01/14/04 08:47 AM (12 years, 10 months ago)

Quote:

simplemachine said:
>i dont shake after innoculating, i have better luck this way.

To each his own. I just like having jars that colonize in a week instead of three or four. I envy your patience!




This is why I am only doing Karo Tek for WBS from now on. Those first ones took for-fuckin-ever.


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Offlinethechemist
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Registered: 09/25/03
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Re: First timer, just innoculated...what a bitch it was [Re: JetBlackNinja]
    #2241038 - 01/14/04 09:20 AM (12 years, 10 months ago)

you cant shake a BRF jar...but you can shake any popcorn/or grain based jars....


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OfflineMekyle7
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Registered: 05/01/05
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Re: First timer, just inoculated...what a bitch it was [Re: thechemist]
    #4200849 - 05/20/05 07:46 PM (11 years, 6 months ago)

That side of the jar thing is a good idea, not flawless but Ill try it next time - I jus had the same problem...30 seconds ago? I pushed hard with one hand while the other stopped it from going too far. It worked ok but Im prolly gunna get like 50% contam cause I got frustrated :/


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Brown Rice Flour, Rye Grain, Vermiculite, Wild Bird Seed

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