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OfflineMycologist217
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COMPLETED SUCCESSFUL GROW---Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style-- * 1
    #22381269 - 10/14/15 10:42 PM (8 years, 3 months ago)

And without further adieu Mycologist217 is back and this time i am doing a side by side grow of PC Penis Envy and PC B+ . I will be inoculating 10 WBS jars with PE spores from a syringe and 10 WBS jars with B+  spores from syringe.

If you are curious about any of this procedure, terminology, or eager to see results or my credentials as an amateur cultivator, I encourage you to check out some of my previously published grow logs.

You can check out my first Penis Envy mono tub ever by clicking HERE. That log is written lab manual style and will provide many answers beginner cultivators have about this procedure.

If you are interested in the PF tek, check out my lab manual style grow log featuring PE MS syringe on PF trek by clicking HERE

Or if you want to see how I got started check out my first ever grow by clicking HERE.

Learning Objectives:
-Demonstrate a novice practical knowledge of WBS jars (preparation, inoculation, colonization, & spawning.
-Demonstrate  a novice practical knowledge of coco coir bulk substrate (inoculation, colonization, and fruiting).
-Successfully prepare, pasteurize, and utilize a casing layer.
-Demonstrate practical knowledge of the technology and science of the Monotub.
-Successfully construct and utilize 2 Monotubs
-Maintain a high rate of contamination free cultivation.
-Observe two strains of the same species grown in separate mono tubs under similar conditions.
-Provide exemplary information on bulk cultivation for new cultivators and curious minds alike.

Materials Needed:
-Pressure cooker
-aluminum foil
-MS Syringe containing spores from unnamed species/strain.
-quart mason jars with rings
-prepared WBS lid (tyvek filter and polyfill stuff inoculation port)
-prepared wild bird seed (WBS)
-coco coir
-vermiculite (bucket TEK show course Verm)
-Jiffy mix (casing straight from bag)
-constructed monotub
            -polyfill
            -sharp knife or shears
            -impermeable tape
            -can liner
-SAB (still air box)
-sanitizing agents (isopropyl alcohol, bleach spray, paper towels, alcohol lamp)
Please let me know if I missed anything.

Welcome back to another mycologist217 grow log. This time we will be cultivating PC Penis Envy and PC B+ using  Doc34's Wild Bird Seed TEK , and The Ohmatic Monotub TEK along with his coir pasteurization TEK. however, I will be using This coir substrate prep TEK (I will still be using "the bucket TEK" for pasteurization); Franks Simple Coir/Verm TEK for substrate prep, and Franks spawning TEK and Franks casing TEK linked in the log below.

~~DISCLAIMER~~ This grow log is written in real time and I want to go ahead and say the syringes I am using are more than 6 months old. While if they are dirty which is very likely, I will be acquiring new ones and will be seeing this grow to fruition no matter what, but it should be noted we are starting with old spores.

I've begun the grow by starting the soaking of my WBS, and constructing my jar lids. More updates coming after inoculations.


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Edited by Mycologist217 (12/12/15 07:08 PM)


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InvisibleLocN9ne
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22381366 - 10/14/15 11:13 PM (8 years, 3 months ago)

Why not go to agar first?


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: LocN9ne] * 1
    #22382632 - 10/15/15 09:49 AM (8 years, 3 months ago)

The learning objectives for this log do not include establishing any new knowledge.


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22387868 - 10/16/15 10:51 AM (8 years, 3 months ago)

Day 0-

Traditionally I call inoculations Day 0. So, because of how much free time I had this week (or lack there of) I decided not to do 20 jars of each Penis Envy and B+, instead I inoculated 8 quart jars with penis envy and 5 quart jars with B+, I suppose I might have to do a smaller mono tub for the B+, but assuming all 8 of my Penis Envy jars reach healthy full colinization, I'll have a decent amount of spawn for the standard 66qt monotub. I have always been assuming that 8 was a little on the high end for spawn ration but also that Penis Envy benefits from a higher spawn ratio. Can anyone tell me if 5 healthy quarts of colonized B+ will be okay for a 66qt monotubs?

Some might want intricate details of the process I followed throughout preparation and inoculation:

I started on Wednesday night....late....I rinsed all my birdseed thoroughly using wire mesh strainers and warm water. After I was satisfied with the rinse I used my 16qt pressure cooker as a basin to submerge the seed. I removed all floaters including all black sunflower seeds. I then left the rest submerged for ~14 hours. On Thursday evening I strained and rinsed these soaked seeds thoroughly and then simmered them without bowling in water for 25 minutes. After that I gave them a final rinse and strain and allowed them to drain dry for close to 90 minutes. When they passed the tissue test, I used a scoop and a funnel to load the hydrated seeds into quart mason jars about half full.

I covered the mouths of each jar with a double layer of Tyvek cut from a set of painters coveralls (<$5), the topped with the prepared WBS lids (1/4" hole drilled in center of lid with poly filled stuffed through and trimmed). After securing the lids with the rings, each jar lid was given a double layer of aluminum foil very snugly crimped about the jar neck. The jars were loaded into my pressure cooker in two rounds and steam sterilized at 15 PSI for 90minutes. The jars were left to cool over night.

This morning the sterilized jars were removed from their cooling places in places in a SAB (Still air box) in a small draftless room. The room had been prepped by dousing with disinfectant cleaner containing bleach, and all tools therein were purged with 70% isopropyl alcohol. A homemade alcohol lamp fueled by 91% isopropyl alcohol was used to flame sterilize the needle of my MS Syringe inbtween inculcation of the jars. Each jar was inoculated with 1< x < 2 mL spore solution.
Each jar was covered with two coffee filter after inoculation which were fastened to the jars via rubber bands. The B+ jars were marked to be made distinguishable, and all the inoculated jars were placed in a closet that stays around 75F to colonize.

I expect to see signs (healthy or otherwise) of growth by Tuesday or Wednesday of the coming week, and I will post pictures to provide examples when growth becomes visible!

Homemade alcohol lamp:
I use a small garlic jar with a cotton ball pulled through the lid with 91% isopropyl as the fuel. Burns clean and red hots the needle in seconds...costs about 5$ total to construct..


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineICEMANOO9
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22389832 - 10/16/15 06:39 PM (8 years, 3 months ago)

That alcohol lamp is a really neat idea


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: ICEMANOO9] * 1
    #22390626 - 10/16/15 09:11 PM (8 years, 3 months ago)

I've used this lamp for every inoculation I've ever done and I doubt I've ever lost a jar due to poor needle sterilization.


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22411327 - 10/20/15 09:18 PM (8 years, 3 months ago)

Day 4-
All five jars that were inoculated with B+ show signs of mycelium growing healthy. 5/8 jars inoculated with penis envy show evident mycelium. Very excited about this timing, and happy my syringes remained viable through 7 months of storage at 70-80 degrees.


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22411669 - 10/20/15 10:52 PM (8 years, 3 months ago)

I started WBS jars last week also. Day 0 being 10/17 using PE MS syringe i've had for about a year. I'm really hoping they turn out, it being my first monotub grow. Cool to know i can follow a pro while starting this journey.


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: sporling] * 1
    #22411872 - 10/21/15 12:22 AM (8 years, 3 months ago)

I am by no means a pro this is only my third monotub ever. I won't be modest, I have had great results, but the pros on these forums are light years beyond my amateur ms spore inoculations. However the purpose of the real time lab style log is to be as helpful and resource rich as possible to new cultivators.

These are examples of what you can expect 3-5 days post inoculation:


If you look closely you can tell the image on the left is a fast growing sample, while the image on the right is much slower with barely visible colonies forming.

-it should be noted that these jars are being incubated at 78F


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Edited by Mycologist217 (10/21/15 09:27 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22412772 - 10/21/15 09:08 AM (8 years, 3 months ago)

I am feeling pretty nervous because i used my last of my PE syringe to inoc 4 WBS jars. 1cc each jar. The WBS i bought turns out when opened its 75% sunflower seed. i should have either went and found rye berries or got a different kind of WBS. I didn't bother scooping the seeds off the top because it would be a 75% waste of $$. I'm hoping for something to show up within the next few days but i know PE is slower than most.


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: sporling] * 1
    #22413536 - 10/21/15 12:28 PM (8 years, 3 months ago)

Day 5-

Because I could see mycelium growing in all jars, I gave each jar a good shake! My grains seemed really nicely prepped: no residual water, and no water rings. Any seeds that had been previously sticking towards the mouth of the jars have been incorporated with the rest of the substrate. I hope to see mycelium patches growing all over the jars within 48-78 hours. I feel I am moving right along schedule for a MS spore inoculation.


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineGreenHornThumb
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22413700 - 10/21/15 01:22 PM (8 years, 3 months ago)

:popcorn:


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: GreenHornThumb] * 1
    #22422245 - 10/23/15 10:07 AM (8 years, 3 months ago)

Day 7-
All 5 B+ jars have recovered from their shake and 6/8 penis envy jars are also looking great! Now it's just a wait until full colonization...maybe a shake here or a flip there...

-observation-...the bottoms of these jars are a heavy concentration of colonization...maybe the spore solution made it all the way down there, or I shook well...but almost all jars are colonizing from the bottom up.



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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22442610 - 10/27/15 10:53 PM (8 years, 3 months ago)

Day 11 [end of day 11] -

Yesterday I gave all jars a second and final shake. Tonight all five b+ jars look awesome and 5/8 penis envy jars look awesome. 3/8 PE show little to no growth and have been isolated from the healthy looking jars. I expect the 10 jars to reach full colonization within the next 4-7 days which means I have some preparations to make. I don't feel comfortable spawning just 5 quarts PE To a full brick of coir and the verm mix for the Tek. I will immediately seek advise on the matter following this post. I am sure the B+ will be fine? I'm going to find out and get appropriate size totes to construct my mono tubs out of....

And now the pics:


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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22449272 - 10/29/15 01:31 PM (8 years, 3 months ago)

Day 13-
Racing toward spawning...colonization happening at great pace!

In other good news, two of three isolated jars boomed to life in the last couple days and are now coloninizing with the intent to be master jars for grain to grain transfer...my first ever.

Picture update:



--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22449288 - 10/29/15 01:36 PM (8 years, 3 months ago)

what's the cloth thing on top of the jars? I hope you have proper filters underneath?


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo] * 1
    #22449300 - 10/29/15 01:39 PM (8 years, 3 months ago)

Quote:

spacechildo said:
what's the cloth thing on top of the jars? I hope you have proper filters underneath?



Those a a little placebo I like to use. They are coffee filters rubber banded around the jar lids, there is in deed a proper Tavel filter under the disk and a poly fill stuffed inoculation port. I just like the coffee filter for....peace of mind.


Edited by Mycologist217 (10/29/15 01:40 PM)


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22464277 - 11/01/15 08:46 PM (8 years, 2 months ago)

Day 16-
3/5 Penis envy and 3/5 B+ jars have reached full colonization with their brethren lying closely behind. I removed the coffee filter safe guards from the less than fully colonized jars to promote gas exchange.

1/3 isolated jars continues to show now growth. 2/3 are now colonizing fine.

Picture (included are isolated jars.)


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22470757 - 11/03/15 12:05 PM (8 years, 2 months ago)

Day 18-

Still waiting on the last couple jars to finish colonizing. They are closing up the hole, but it is taking a while...don't want to make the fully colonized jars wait too long on their brethren to finish.
Picture update:



--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22470851 - 11/03/15 12:24 PM (8 years, 2 months ago)

looks like there's a bit bacteria in there to me, thick white growth thats going slow.

try shaking them and dispersing the grains well, if they dont wanna recolonize properly you dont want them in your tub anyway :super:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22471363 - 11/03/15 02:34 PM (8 years, 2 months ago)

Really wish people would use the correct forums.
anyway.. Gj.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Arush]
    #22471451 - 11/03/15 02:49 PM (8 years, 2 months ago)

mush cult is an excellent choice of forums to show your mushroom cultivation in! :super:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22472199 - 11/03/15 05:27 PM (8 years, 2 months ago)

Very nice I'm definitely watching.


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OfflineMrSturgill
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22473990 - 11/03/15 11:24 PM (8 years, 2 months ago)

Quote:

spacechildo said:
mush cult is an excellent choice of forums to show your mushroom cultivation in! :super:




I think Arush means it should be in grow logs... not quite sure what the big difference is personally :shrug:

Watching


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrSturgill]
    #22474278 - 11/04/15 01:28 AM (8 years, 2 months ago)

Quote:

MrSturgill said:
Quote:

spacechildo said:
mush cult is an excellent choice of forums to show your mushroom cultivation in! :super:




I think Arush means it should be in grow logs... not quite sure what the big difference is personally :shrug:

Watching




If that's what he means, that's incredibly petty.

Mushroom Cultivation (67 viewing)

Mushroom Grow Logs and Pictures (1 viewing)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22474494 - 11/04/15 04:46 AM (8 years, 2 months ago)

:popcorn:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: thelivingfreekshow]
    #22497257 - 11/08/15 06:31 PM (8 years, 2 months ago)

Day 22-23
It's spawn day. Honestly I am not very optimistic. The long colonization time doesn't really have me in high hopes, but the seed is fully colonized and smells fine. We are using the bucket trek as indicated in the intro, and we are using some pretty reputable supplies. More pictures coming later in the night:



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22497308 - 11/08/15 06:42 PM (8 years, 2 months ago)

post a pic of teh jars not in the box, there were a cpl that looked good 5 days ago maybe you rather wanna just use them or g2g them
and not mix them with the bacterial ones?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22497324 - 11/08/15 06:46 PM (8 years, 2 months ago)

Quote:

Quote:

MrSturgill said:
Quote:

spacechildo said:
mush cult is an excellent choice of forums to show your mushroom cultivation in! :super:



I think Arush means it should be in grow logs... not quite sure what the big difference is personally :shrug:

Watching



If that's what he means, that's incredibly petty.

Mushroom Cultivation (67 viewing)

Mushroom Grow Logs and Pictures (1 viewing)




Yes because everyone's in the same wrong mind frame. Why even have different forums? Don't QQ


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Edited by Arush (11/08/15 07:01 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22497352 - 11/08/15 06:51 PM (8 years, 2 months ago)



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22497418 - 11/08/15 07:04 PM (8 years, 2 months ago)

hmm, a bit grainy pics and its hard to tell when they've grown this much/overgrown,
but if we say there's 5 jars you can see in the 1st pic I would def not use jar 1 with the others.
no 2 looks a bit suspicious but it may just be the quality of the pic, the myc looks "grainy" and a bit off but as I said hard to say 100% sure.
jar 3 has very suspicious growth towards the bottom and some off color going on above that again. would not use.
if jar 4 has been ready/all white for some days that may explain all the fuzz on top, doesnt look too bad IMO.
jar 5 has some bacteria but not much Imo.

I wouldn't use anything from pic 2, maybe the third, but i'd give it a shake first and see how it recovers.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22497450 - 11/08/15 07:08 PM (8 years, 2 months ago)

pics from 5 days ago:

Quote:

Mycologist217 said:
Day 16-
3/5 Penis envy and 3/5 B+ jars have reached full colonization with their brethren lying closely behind. I removed the coffee filter safe guards from the less than fully colonized jars to promote gas exchange.

1/3 isolated jars continues to show now growth. 2/3 are now colonizing fine.

Picture (included are isolated jars.)





pic 1: 1 is fucked, 2 and 3 have bacteria 4 maybe good if the myc dont look any worse now. its borderline but looks good to me.

pic2: 1 is fucked (same jar as pic1?) 2 and 3 dont look bad to me, 4 has a bit bacteria in it. wouldnt spawn with good jars.

pic 3: 1 is still quite young, i'd give it a 50/50 shot, 2 looks very off to me.

bacteria in jars doesnt necessarily mean garbage, you can try to spawn them to trays or mini monos if you want to.

edit; dont throw it away yet because i said so regarding some jars,
its really hard to tell through a picture, maybe someone chimes in later and has comments on my diagnoses.


Edited by spacechildo (11/08/15 07:10 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22498718 - 11/09/15 12:12 AM (8 years, 2 months ago)

Late AF Day 23-
Spawned the penis envy seed from six jars. All jars smelled exactly as they should, and I feel a lot more optimistic now that it is in the final resting place. I have the substrate pasteurizing for the b+ tub right now.....long night ahead....



Pink for penis envy, got blue for b+


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Edited by Mycologist217 (11/09/15 12:14 AM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22499656 - 11/09/15 09:48 AM (8 years, 2 months ago)

Day 24-
This morning rolls in to find me very tired. When my alarm went off shortly after 5AM to alert me to finish off the second tub, I almost couldnt remember in those first few seconds why I was up at 5:11...

Upon final inspection and smelling of the B+ seed, one suspicious looking Qt of seed was sporting a very small, but all the same very there fruitbody....rats....jar was discarded, and the Monotub was spawned with just 4 quarts of B+ seed.

On the flip side, the Penis Envy tub was spawned with 6 nicely smelling quarts of fully colonized seed. Everything had a very pleasant alkaline earthy smell...no sweetish or funky odors.

The four quarts of B+ appeared to be spatially appropriate, and providing they are clean (contamination free) at this point I suppose I am in store for a first flush at least of B+ and Penis Envy.

With the only work left being to put the tubs into fruiting conditions, I feel pretty good about the progress of this grow thus far. It is the first grow i've done in my new house, which was a big hurdle to me, and even during the excruciatingly long grain colonization period....I was not optimistic. But, I am putting the negative vibes to the curb, and ushering positivity for my tubs from here on out.

In other news, more inoculant has been acquired, and I plan on moving into agar work in February.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22504572 - 11/10/15 11:07 AM (8 years, 2 months ago)

Day 25-
Both tubs seem to be taking to colonizing like ducks to water. I feel way more optimistic this side of spawning....
Obviously I'm not going to be cracking the kid every day for pictures, maybe closer to fruiting I'll give a in the tub shot, but for now these pictures through the monotub will have to do.

B+ on Left.    Penis Envy on Right


This is what you can expect 24-36 hours post spawning.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22505002 - 11/10/15 01:04 PM (8 years, 2 months ago)

How long does it usually take for the whole monotub in to be fully colonized?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Shreality]
    #22505040 - 11/10/15 01:13 PM (8 years, 2 months ago)

Usually between 5-7 days when using a coir based substrate. Genetics plays a strong role in it as well.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22505044 - 11/10/15 01:15 PM (8 years, 2 months ago)

and spawn ratios. I think munch or mad season colonized one in a day, some crazy 2:1 ratio or more!


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22505080 - 11/10/15 01:25 PM (8 years, 2 months ago)

:mindblown:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22505187 - 11/10/15 01:52 PM (8 years, 2 months ago)

:super:

it was munch, 100% colonization in 1 day

strong genetics, proper moisture content of the substrate, higher temps and # of inoc points matters a lot.
also having no bacteria in your spawn speeds up colonization with days!


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22505224 - 11/10/15 01:58 PM (8 years, 2 months ago)

Quote:

spacechildo said:
:super:

it was munch, 100% colonization in 1 day

strong genetics, proper moisture content of the substrate, higher temps and # of inoc points matters a lot.
also having no bacteria in your spawn speeds up colonization with days!



Damn that's awesome!! Munch didn't post the end results tho :frown:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22505261 - 11/10/15 02:06 PM (8 years, 2 months ago)

haha, it was 5:1 ratios! he'd have to water that like crazy I suspect, at least if he wants to get something out of all those grains.

its basically just grains recovering and not having to travel far to reach the next recovered grain/noc point.

once myc gets a hold of the sub its not much difference in colonization times between 1:2 and 1:4 anyway.

clean spawn, proper moisture in the sub...perfect!


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22505358 - 11/10/15 02:27 PM (8 years, 2 months ago)

:hellyeah:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22509229 - 11/11/15 12:45 PM (8 years, 2 months ago)

Day 26-
Picture update of colonization this is after two full days since spawning.
B+ on left PE on right


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22513157 - 11/12/15 09:41 AM (8 years, 2 months ago)

Day 27-
We are in day four of tub colonization and I suppose realistically both tubs are fast approaching full colonization. Haha,I know 6 days or so isn't bad at all for tub colonization especially with the spawn ratios I used but the anxiety has begun to set in and I am feeling in my head like this is just taking forever. On my first monotub it took a good 15 days after fruiting conditions were introduced to start getting pins. I really hope it goes a little faster this time, but above all ny love and good vibes go into hoping for just success. Picture update tomorrow for better indication of growth.

Quick question....i want to build a rig to hold a camera inside on of the mobotubs to make a time lapse once pinning starts. Are there any precautions I need to take? I know touch screens are sensational bacteria breeding ground...


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22513299 - 11/12/15 10:20 AM (8 years, 2 months ago)

Does itusually take that long for pinning to occur in a mono tub? And whatever camera you use just be sure to sterilize very well to avoid contamination. Or you could always make a small hole in the top big enough for the lense to fit through and then seal it up


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Shreality]
    #22513524 - 11/12/15 11:23 AM (8 years, 2 months ago)

Quote:

Shreality said:
Does itusually take that long for pinning to occur in a mono tub? And whatever camera you use just be sure to sterilize very well to avoid contamination. Or you could always make a small hole in the top big enough for the lense to fit through and then seal it up




I don't know if there is a "usual" time for mono tubs to start pinning...I suppose being spot on has a lot to do with how well the monotub is dialed in and what the fungus wants to do. I assume more experienced cultivators get better and better at reading their fungus and have shorter and shorter times between introduction of fruiting conditions and pinning.

You do not sterilize things like cameras. You sanitize them. As a cultivator it is very important to understand the difference between steril work and a sanitized area.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22514093 - 11/12/15 01:43 PM (8 years, 2 months ago)

Oh I gotcha. Sorry for all the questions, I'm trying to figure out this mono tub thing because that's what im going to try next.
Oops sorry but you know what i mean. It would be really cool to see a time lapse of the pinning process


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Shreality]
    #22517115 - 11/13/15 08:18 AM (8 years, 2 months ago)

Day 28-

Picture update:
Penis Envy on Left. B+ on right



The b+ tub is being stored in a room several degrees colder than penis envy, should I move them to the same spot? Stacking these is okay?


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Edited by Mycologist217 (11/13/15 08:20 AM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22517173 - 11/13/15 08:41 AM (8 years, 2 months ago)

A lot of people stack their tubs, just make sure you have a light source near the tubs on the side.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22524447 - 11/14/15 08:43 PM (8 years, 2 months ago)

End of Day 29-

So from my amateur eye I'd say the Penis Envy tub is 24-48 hours from being introduced into fruiting and the B+ tub is maybe 60 hours away....but I'm definitely no pro at this so I need your help.

Please tell me what you think on the status of these tubs

PENIS ENVY


B+


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22528622 - 11/15/15 06:20 PM (8 years, 2 months ago)

Day 30-

I'm ready to case my PE monotub as per the recommendation of the trusted cultivators on this forum....I went to get casing supplies and I got it....but then I went to Wal-Mart to buy a Star Wars chess set for a Christmas gift....I strolled through their garden section and there it was:



So I think this is going to be what I use for my casing....Frank loves it so I love it too I suppose....

Anyway I'm about to use Franks Casing Tek.

I am very scared about this pasteurize get process


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22528739 - 11/15/15 06:55 PM (8 years, 2 months ago)

all you need is to take things slow and use a thermomether. remember the core temps will keep rising even after you turn the heat off.
if temps are climbing too fast for you throw a pint of cold water in the pot. first time is kinda crazy, at least mine was, taking the pot off the heat, adding cold water, trying to find the settings that did the trick for me. 2nd time is a breeze and after that you barely dont need the thermomether anymore :tongue2:

I believe in you man! :cheers:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22529728 - 11/15/15 09:42 PM (8 years, 2 months ago)

I'm currently pasteurizing my Jiffy mix casing that Frank  says to use straight out of the bag. The temperature has been around 155F since  shortly after I turned my stove off at 140F and we have 27 minutes to go at the time of this post. I feel more comfortable about this pasteurizing process at the moment...I keep telling myself that if the Bucket Tek works then watching this be at 155-160 for close to 55minutes has got to....but really I am eager to see this Penis Envy tub fruit, and I am feeling pretty good about the decision to case...



I am eager to learn more about between flush procedure as I ditched my first tub after the second flush made barely any progress, and it was not cased.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22529816 - 11/15/15 10:05 PM (8 years, 2 months ago)

you got it man! :thumbup:

just spray the tub with water. you can wait a few days if the tub just sits there or mist it right after harvesting if there's new younglings coming up.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22529859 - 11/15/15 10:12 PM (8 years, 2 months ago)

Looks like you've got it right, just make sure those temps don't exceed 165f for too long or you will be partially sterilizing your substrate.

And for your next round of pasteurizing jars, I recommend using a digital meat thermometer. This way you can put the lid on the PC and your jars will heat up more evenly as you watch the temps from outside. It makes the process a whole lot easier! :thumbup:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22530081 - 11/15/15 11:18 PM (8 years, 2 months ago)

The B+ will not benefit from a casing layer?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22530400 - 11/16/15 02:14 AM (8 years, 2 months ago)

well documented


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OfflinePsilosoulful

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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22530690 - 11/16/15 07:06 AM (8 years, 2 months ago)

Quote:

Mycologist217 said:
The B+ will not benefit from a casing layer?



All cubes will benefit from a casing layer :awesomenod:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22531816 - 11/16/15 10:27 AM (8 years, 2 months ago)

Day 31-
FRUITING!!!

So both tubs were introduced to fruiting conditions this morning! The Penis envy tub was cased with the jiffy seed starter mix containing Pete, vermiculite, and lime that I pasteurized last night and cooled in my SAB over night before applying this morning. Both tubs are displaying condensations patterns consistent with Frank's  example of proper FAE...now it's just a waiting game.

The casing of the penis envy made me nervous because so many threads have been read saying that this Pete moss and lime isn't exactly easy to keep contam free and if I had gone with no casing I was pretty much guaranteed a healthy first flush....but I want to improve my skill, so I took a step up. I did not case the B+.



Let me know how the casing looks please.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22532017 - 11/16/15 10:49 AM (8 years, 2 months ago)

The casing looks good, you just want a nice thin layer over the surface, no need to break out the ruler or anything :lol:

And as long as your pasteurization procedures were good, you don't have to worry about it contaminating. :goodluck:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22537482 - 11/17/15 12:56 PM (8 years, 2 months ago)

Day 32-

So now we are coming down to the waiting game....the first time I did a monotub it took like 16 days to start pinning after fruiting conditions were introduced, but i did not case that. I would imagine casing slows down pinning since the peat and verm will get somewhat colonized?

I am very curious to see the difference casing Penis Envy tub makes verses not casing it. When I did not case it I had a 2.5 oz first flush about....I know genetics obviously play a huge role in fruiting behavior but I want to imagine the environment created by the lime and peat and verm will encourage extremely widespread pinning.

I am also eager to clone a fruit to agar since my MS spore inoculations are not going very well at all.

Pictures:
Penis Envy (cased) on left, B+ (not cased) on right.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22540991 - 11/18/15 09:14 AM (8 years, 2 months ago)

Day 33-

Just a couple days into fruiting now. The PE mycelium is starting to make work of the casing layer and I would imagine that if I cased then continued colonizing before fruiting then I would be fruiting this morning anyway.....usually I'm pretty good about curbing my anxiety waiting on pins but right now I am very eager to see it happen. The results displayed by people using the jiffy mix to case their PE are so astounding. I know I inoculated from a MS spore syringe but THE source has never provided me with weak genetics. I would guess spore syringes are made from fruits with decent genetics by practice. At least I have an organic chemistry and cell bio final to study for this week.

Pics:


Pink for PE. blue for B+


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22545867 - 11/19/15 09:31 AM (8 years, 2 months ago)

Day 34-
I think the PE tub is starting to knot. The B+ looks less colonized today than it did yesterday....weird...see pics then compare to see what I mean....I missed the B+ tub last night so maybe it just looks different because more moisture? Not really worried about it I suppose...

Let me know what you think of things so far, PE  is the cased tub and B+ is not cased:


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Edited by Mycologist217 (11/19/15 09:36 AM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22546689 - 11/19/15 12:51 PM (8 years, 2 months ago)

Eagerly awaiting results.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: TriggerFish]
    #22549492 - 11/19/15 10:44 PM (8 years, 2 months ago)

So the PE is just tearing away at that casing layer....very evenly across the whole tub...do I need to do anything? Experienced cultivators with PE on WBS to coir/verm/gypsum cased with jiffy mix.


PE is left two photos. Right two are just pic update of B+

Once again to experienced cultivators, do I need to do anything like "patch" that casing?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22550699 - 11/20/15 09:26 AM (8 years, 2 months ago)

Day 35-
Just a picture update and the sa,e question as last night:

Penis Envy, do I need to patch this up or anything or just let it be itself? First time casing so I don't know:


B+ photo update:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22551180 - 11/20/15 11:25 AM (8 years, 2 months ago)

Quote:

Mycologist217 said:
Day 35-
Just a picture update and the sa,e question as last night:

Penis Envy, do I need to patch this up or anything or just let it be itself? First time casing so I don't know:


B+ photo update:





I believe at 20?% casing colonization you introduce to fruiting?  At least it was what I did and had a nice flush


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: GreenHornThumb]
    #22553263 - 11/20/15 09:51 PM (8 years, 2 months ago)

Oh man I am getting obsessed with this PE tub....I am so happy my university lets out the whole week for holidays! i wonder if I will ever get so involved cultivating that I don't even notice pinning times anymore, but right now it's almost unbearable....like its my first grow all over again....but I feel like my tub looks great....it looks awesome...and I hope it fruits just as amazingly....I a, worried the B+ tub will know I don't care as much about it so I try to include it as much as I can, but really my mind is on this cased PE tub during all my waking hours...


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22557000 - 11/21/15 07:22 PM (8 years, 2 months ago)

Day 36-

The B+ tub is pinning!!!!! :mushroom2::mushroom2::stoned:
Ironically it has pinned on day 36 where the penis envy monotub I grew in April also pinned on day 36! Weird...especially as these jars had a full ten days longer in colonization, but oh well...

There are dozens of extremely small very newly debuted pins and they are impossible to see from a far so I got a shitty picture up close....I don't know why the iPad's camera is so shitty....probably so they can upgrade the next model...when I had an iPhone the pictures were way way better....


The PE tub is doing some serious work on the casing layer but it is starting to know up really nicely and I think it will be just a couple days (more like hopeful) behind its brother B+


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22557353 - 11/21/15 08:47 PM (8 years, 2 months ago)



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22561248 - 11/22/15 08:18 PM (8 years, 2 months ago)

Day 37-
Obviously things are very exciting right now with the B+ tub. A bunch of new pins appeared today and I am expecting more and ,ore over the next couple days. I gave the tub a 180Degree spin as the pins seemed concentrated toward one end of the tub. Hope that's a good play..,,the pins that were there last night were a little more visible tonight with only one "cluster" being present.



Hopefully I end up getting decent mass in the first flush, but I understand ms inoculation is a crapshoot

Penin Envy tub is knotting up like crazy and the casing layer almost fully colonized....ready for a great flush (HIGH HOPES :stoned:)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22564966 - 11/23/15 07:07 PM (8 years, 2 months ago)

Day 38-
I really need some advice on how to feel about this. The penis envy tub is glittered with yellow metabolites...I don't know what it means or what to do about it as this is my first encounter. Please take a look and let me know what's up please:


In other news B+ is starting to pin more and more:


Again please help me out on the PE tub.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22570465 - 11/24/15 09:02 PM (8 years, 2 months ago)

Day 39-
Tonight I'm okay I guess...I've been searching and searching through the forums trying to find out what's up with these yellow drops of water....most of what I've found the grows turned out fine....the substrate is knotted like crazy.......I am just praying with all my possible high hopes that it turns out okay....forty days to run into problems now isn't cool, but I'm cheerful I suppose. Increasing air flow instantly resulted in more primordia so we'll see how next couple days go:


B+ is pinning more and more buts not exactly the wonderland full canopy I was hoping more....I guess the next couple days will also a lot about the B+ tub...


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22570745 - 11/24/15 10:10 PM (8 years, 2 months ago)

whats your substrate?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: cronicr]
    #22570753 - 11/24/15 10:12 PM (8 years, 2 months ago)

Quote:

cronicr said:
whats your substrate?



WBS spawned to coir/verm/gypsum cased with jiffy mix containing 50-60 peat moss to verm and some lime


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22570763 - 11/24/15 10:15 PM (8 years, 2 months ago)

nice i wouldn't sweat it man:cool: just keep doing what you are doing because you're doing great, metabs can be from lots of shit from bacteria to an over colonized sub to temps to absolutely nothing more then it eating


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: cronicr]
    #22574787 - 11/25/15 07:01 PM (8 years, 2 months ago)

Day 40-
Something about the way Cronicr compliments a guy really calmed me down. I am seriously waiting on baited breath for the PE tub to pin and I am grateful that the B+ is doing work.

I am happy that more and more pins keep popping up in the B+ tub..lif every single primordia became a fruiting body I would be in extasy. There are lots and lots of pins on the side of the tub and the middle is filling out, favoring one end of the tub but the other end has a few...I a, curious to see how long it forms pins for before they are all just growing fruits with no new ones forming...I see people's pictures of hundreds of pins coming up together....obviously this is not going to be what happens here...or could it still happen?

Keep sending good vibes to the PE tub:


I plan using a larger fruit from the cluster on the the right picture to take a tissue transfer (clone) from. Is this the type of formation I want to be taking samples from?


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Edited by Mycologist217 (11/25/15 07:03 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22575323 - 11/25/15 09:17 PM (8 years, 2 months ago)

Quote:

Mycologist217 said:
I plan using a larger fruit from the cluster on the the right picture to take a tissue transfer (clone) from. Is this the type of formation I want to be taking samples from?



Yes, that'll be fine. Take a clone of the fastest/largest growing mushie :thumbup:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22579111 - 11/26/15 04:57 PM (8 years, 2 months ago)

Day 41-
Tons and tons of B+ pins....a couple that are clearly going to abort....and a few looking like they need more FAE....but there's lots of them...


In other news....THE PENIS ENVY TUB IS BLOBBING AND PINNING!!!!!
No real pins yet, but I am sure a couple are there....several freckled blobs made their way up though....teeny tiny...they remind me of birds eggs for some reason....sure we'll get more and more in the next couple days but hopefully we get lots and lots of pins....little dick shaped mushroom pins.

So I want to take a couple seconds to talk about my grow room....give some insight to the newer cultivators here, so first....picture:


You'll notice that in the lower left hand corner [of the room] is a room fan. I had it on medium until I got all these metabolite pools on my PE tub a few days back. I turned it to high to increase FAE. You'll see the fan is pointed into the wall and that the tubs are positioned to where the long ends form a right angle to direction of the fan....then in the next photo you'll see a completed unshuttered window that's open. This window has been open since fruiting started as a means to maintain FAE and not just move stale air around the room. The temperature in this rooms hovers around 70-74F....seeing good results so far fruiting stuff in this room.


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Edited by Mycologist217 (11/26/15 05:07 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22581336 - 11/27/15 08:51 AM (8 years, 2 months ago)

Day 42-
Havana so today I feel pretty good about this project....honestly the PE Tub looks....gross....it's covered in those pools of yellow fluid....not covered but there are lots of drops of metabolite liquid...and now it has those teeny tiny freckled blobs....everywhere....the first time I did a PE tub I only got the blobs on the sides of the tub....it seems the casing layer did exactly what everyone says it doesn't do....I feel like I'm in for a great flush....every so often amongst the blogs and metabolites little penis envy pins are starting to pop up....sometimes these "blobs" are ending up being pin caps....


B+ is really starting to be something to look at....


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22584195 - 11/27/15 09:37 PM (8 years, 2 months ago)

Late Day 42-
:prettyflyforawhiteguy:
I wish I was into agar back in April......this penis envy tub is acting so so similarly to the classic examples of Penis envy tubs spread wide across this forum....the pins that are there are mutantesqe....ugly little freckled things.....where the PE I grew in April was much more....Tame?
PE pins from April:


Now....what in the hell happened here?  My current PE tub:

...li cased this time....not in April,..,it's weird...but I'm excited....tell me how it looks...

B+ Going absolutely BONKERS....


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Edited by Mycologist217 (11/27/15 09:38 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22584502 - 11/27/15 11:25 PM (8 years, 2 months ago)

:manofapproval:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: cronicr]
    #22584699 - 11/28/15 01:02 AM (8 years, 2 months ago)

I thought they were blobs....but they are not....they are pincaps....almost all of them are mushrooms....


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22584860 - 11/28/15 02:51 AM (8 years, 2 months ago)

Most of those are in fact blobs.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole] * 1
    #22584875 - 11/28/15 03:10 AM (8 years, 2 months ago)

Quote:

Inocuole said:
Most of those are in fact blobs.




I think you're wrong here....


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22584877 - 11/28/15 03:11 AM (8 years, 2 months ago)

Time will tell.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22585995 - 11/28/15 11:40 AM (8 years, 2 months ago)

Is it normal for it to look that yellow?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Shreality]
    #22586270 - 11/28/15 12:50 PM (8 years, 2 months ago)

Quote:

Shreality said:
Is it normal for it to look that yellow?




Everything I have found about the pooling metabolites using the search function has led me to believe it just happens sometimes and isn't a problem....maybe an indication that it has a mobster flush coming..
.plus cronicr told me not to worry...so :whathesaid:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22587071 - 11/28/15 04:03 PM (8 years, 2 months ago)

A buttload of metabolites are not an indication that a monster flush is about to come through.  It's an indication that the mycelium, for whatever reason, feels the need to produce an antibiotic secretion.  Usually, because of something... biotic.  Go figure.

I always keep a close eye on tubs that go yellow because mold usually follows.  Spawning to manure can have that effect as well, depending on a few factors, not sure what your bulk sub is here.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: cronicr]
    #22587729 - 11/28/15 06:52 PM (8 years, 2 months ago)

Day 43-
Mostly a photo update...but I want to help some of the new cultivators reading this log understand why I am not concerned about the load of metabolites in the PE tub...
1. They showed up a week ago and nothing's has turned green or done anything strange since...
2. Upon initial encounter I searched the shroomery forums before bringing it to the experienced cultivators, and I found when I searched "pool metabolites" and other keywords like Penis Envy Monotub all the similar cases I could find were given the recommendation to fruit or just keep going....several providing examples of success with these metabolite pools....then I found this post by RR:
Quote:

RogerRabbit said:
It looks like metabolites, but there's no such thing as myc piss.  Metabolites serve two functions.  One is to help break down the substrate with solvents, and the other is an antibiotic function to help fight off competitors.  Mycelium doesn't urinate, and the metabolites are not a waste product, despite what you read elsewhere.  Penicillin is produced from the metabolites of another species of fungi.  You certainly wouldn't ask your doctor for a shot of myc piss if you had the clap.
RR




And this:
Quote:

cronicr said:
nice i wouldn't sweat it man:cool: just keep doing what you are doing because you're doing great, metabs can be from lots of shit from bacteria to an over colonized sub to temps to absolutely nothing more then it eating




So I felt comfortable with continuing fruiting and I'm glad I did because like I said no contamination has made itself apparent and this pin porn is frankly....crazy (to me....first encounter with anything like this:


And I guess some B+ too:


Feel like I'm going to have a pretty decent yield for my first flushes of each Tub


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22587753 - 11/28/15 06:56 PM (8 years, 2 months ago)

Well feel comfortable fruiting it, sure, but it doesn't happen for no reason.  Sometimes it can happen for no determinable reason, but it doesn't just produce metabolites for no good reason at all.  Given that there must be SOME reason, it's important to keep an eye on it at least.  Mold won't usually hit it til after the first flush.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22591043 - 11/29/15 03:30 PM (8 years, 2 months ago)

Inocuole! I've missed you! where have you been!?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrSturgill]
    #22591293 - 11/29/15 04:45 PM (8 years, 2 months ago)

Is this the running joke now?  We're gonna pretend like I've been missing every time I pop up in a thread?  :lol:

I've posted every day for the last week or so at least.  I can do some more shitposting than normal if that helps define my presence.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22591555 - 11/29/15 05:45 PM (8 years, 2 months ago)

Day 44-
Photo update only today:
B+:


Penis Envy:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22591604 - 11/29/15 05:57 PM (8 years, 2 months ago)

That is some of the blobbiest, spottiest PE I've ever seen.  I get some blobs, a few of them with speckles, but that's just crazy.  Might not yield a ton but it's got a good chance of completely flooring you when you eat them.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22591625 - 11/29/15 06:01 PM (8 years, 2 months ago)

Quote:

Inocuole said:
That is some of the blobbiest, spottiest PE I've ever seen.  I get some blobs, a few of them with speckles, but that's just crazy.  Might not yield a ton but it's got a good chance of completely flooring you when you eat them.




Yeah it looks very very different compared to the Penis Envy I grew in April (pictured in my signature) I guess it's just the result of using the jiffy mix casing layer as I got the spores from the same exact source and they had the same spore print number on them...super crazy....this is the pin set from my last tub:


I know prints dont have much to do with genetics...but still way different.


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Edited by Mycologist217 (11/29/15 06:02 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22591674 - 11/29/15 06:12 PM (8 years, 2 months ago)

Well if anything a jiffy casing should get them looking more normal, that's the last thing I'd have expected as a result. :shrug:

Genetic variation can be a bit of a mindfuck.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22591697 - 11/29/15 06:17 PM (8 years, 2 months ago)

Should I take a tissue transfer to agar with this one?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22591758 - 11/29/15 06:32 PM (8 years, 2 months ago)

Uhhhh if that's the genetics you're after I suppose. :lol:  I wouldn't, it's a little too gnarly for me.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22592857 - 11/29/15 11:50 PM (8 years, 2 months ago)

Just super excited right now...really can't help it....


I am about to explode over this:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22593472 - 11/30/15 06:14 AM (8 years, 2 months ago)

You need to figure out why that one half of the B+ is fruiting so much better and do the same thing to the other half.  Is one side close to a wall?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22593740 - 11/30/15 08:25 AM (8 years, 2 months ago)

Quote:

Inocuole said:
You need to figure out why that one half of the B+ is fruiting so much better and do the same thing to the other half.  Is one side close to a wall?




It's not fruiting so much better as first....you can see in these pictures:



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22594861 - 11/30/15 02:04 PM (8 years, 2 months ago)

Day 45- FIRST FLUSH HARVEST


This is the last shot of the B+ before it started getting harvested:


I pulled 1115 wet grams off with this remaining still to mature:


I just ate these for safety and potency testing:


And I got the remaining harvested fruits into a dehydrator:


I hope I'm in for a good night


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22598701 - 12/01/15 10:51 AM (8 years, 1 month ago)

Day 46-
So these were some of the more mellow fruits I've cultivated, not very visual, but extremely uplifting and long lasting. I'm trying to think of what to do to prepare for the second flush. I've heard people say that flipping the substrate is an excellent option? What do the experienced cultivators think?

Harvested and Dried B+ Fruits:


Meanwhile the PE tub is simply going bananas.....these things are growing and as of this morning there are no signs of warts yet so hopefully I have at least another full couple days til maturity. There are some blobs of significant size now and I cannot wait to safety potency test that!:


Now that I am reaching the point where this grow can definitely be considered a success I want to recap a few things we've learned this grow and offer a but more insight to the growing parameters this grow was cultivated within.

Firstly we learned that MS spore syringes that have been sitting on the shelf for more than 7 months can be safely inoculated directly to WBS and yield admirable results. We have produced case evidence (a good pun) that applying a casing layer (in this case Jiffy Mix seed starter brought to field capacity then pasteurized) to penis envy may in fact reduce blobbing and increase pin load. I have used painters coveralls that DO NOT say Tyvek on the packaging as my filter layer successfully now in over three dozen WBS jars, and finally as always we confirmed that maintaining a positive attitude and pouring positive vibes into your project is the best way to insure success!

Growing Parameters:
The house this project was cultivated in is by no means spotless, I do my best to take extra care when cleaning and prepping my jars, and honestly the kitchen is probably the cleanest room in the house.  The Still Air lab I use has not let me down to date to any significant level. It is in a walk in coat closet that has been reserved only for sterile work. After the point of inoculation of the jars very little care was taken to keep them in a "sanitized" environ as the just colonize on shelving inside a closet in the floor above the still air lab. Two large dogs have completely free roam of my house including my "grow room" which is just my spare bedroom on the second floor....pictures above reveal not so tidy carpet and a wide open window. All water used to mist was tap water and the mister was "sanitized" once before initial fill. I do not tell you all this to highlight what a mess it is but to show new cultivators that hands down proper sterile inoculation procedure is the most important part. If you can inoculate your sterile spawn cleanly....you almost can't lose....Any other questions you have about this cultivation log feel free to ask.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22600389 - 12/01/15 06:05 PM (8 years, 1 month ago)

Wait... warts?  What?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22603517 - 12/02/15 10:40 AM (8 years, 1 month ago)

Day 47-
Photo update of PENIS ENVY? Probably will harvest tonight:


Very excited with these results


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22610128 - 12/03/15 06:53 PM (8 years, 1 month ago)

How many flushes do you think you could get out of these monotubs?


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Shreality]
    #22610176 - 12/03/15 07:06 PM (8 years, 1 month ago)

Nobody's guess is going to be worth a shit, it's up to the tub how much it wants to put out.  With a first flush like that, probably not a whole lot more.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole] * 1
    #22611003 - 12/03/15 10:15 PM (8 years, 1 month ago)

Day 48-
I harvested the Penis Envy tub and dried it throughout the day. I ended up with right at 6 oz dried! Pretty excited! In the meantime the B+ tub is definitely starting to pin again!




I did not tear up the PE substrate hardly at all and I am hopeful for a decent second flush!
Also I built and tested a large capacity dehydrator!



I built this for 55$ total from one 8x4 piece of plywood.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22611021 - 12/03/15 10:19 PM (8 years, 1 month ago)

Great job!


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: cronicr]
    #22611183 - 12/03/15 11:10 PM (8 years, 1 month ago)

nice man!!! thats one bad ass grow iam on my 1st grow now hope to get results like this in the future :mushroom2:


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Edited by MrTrips31 (12/03/15 11:11 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrTrips31]
    #22611248 - 12/03/15 11:34 PM (8 years, 1 month ago)

Awesome job! May I suggest moving your dried mushies from the jars and into a vacuum seal bag with a silica pack? I don't suck all the air out, just let the silica pack do its thing, but you'll be able to store it a hell of a lot easier. Like you, I had jars filling up my closet. Now I have them conveniently packed up in 2oz lots, labeled and stored in a tub. Best decision I ever made!

Look forward to seeing more of your progress.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Kalistis]
    #22612302 - 12/04/15 10:04 AM (8 years, 1 month ago)

Thanks everyone! As always none of this would be possible without the supporters of viewers like you! The shroomery has always been and will always be my most valuable cultivation resource!

Day 49-
Photo update....second flush pinning for B+...looks like it's gonna be a dinky one:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22612375 - 12/04/15 10:28 AM (8 years, 1 month ago)

dont take this in a negative way but this pic is overkill incarnated :lol:



just thought it was funny. gonna look awesome filled up tho! :rockon:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #22612503 - 12/04/15 11:06 AM (8 years, 1 month ago)

Quote:

spacechildo said:
dont take this in a negative way but this pic is overkill incarnated :lol:



just thought it was funny. gonna look awesome filled up tho! :rockon:





Hahaha I actually used this picture to show that there was some housing around the fan so that is sucks in cold air from the intake holes in the bottom and pushes hot air up through the top...didn't realized that mushroom was there....


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22622484 - 12/06/15 05:38 PM (8 years, 1 month ago)

Day 51-
The second flush of B+ is coming in nicely now and I'm going to have some pretty meaty fruits by the look of it! I have decided I will flip the substrate for a chance at a third flush!  Meanwhile the PE substrate is recovering still and I'm keeping it we, hoping for a few large AF second flush Penis Envy fruits!

B+ Second flush tonight:



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22623818 - 12/06/15 09:50 PM (8 years, 1 month ago)

looking nice man :mushroom2:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrTrips31] * 1
    #22628501 - 12/08/15 02:06 AM (8 years, 1 month ago)

Day 52-
I got a lot of the second flush harvested now and I tested out my home built dehydrator at some capacity and it works really really well! I believe the air is really dry inside and that helps a ton!

Still waiting on the PE tub to sprout some second flush pins.....I will flip the B+ for its third flush,

Photos of some capacity in the dehydrator:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22628644 - 12/08/15 03:37 AM (8 years, 1 month ago)

nice man love the set up for drying them..:mushroom2:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrTrips31]
    #22647424 - 12/12/15 07:07 PM (8 years, 1 month ago)

Day 57-
Well we did end up flipping the B+ substrate....what a fiasco.
-soaked in water for a good hour before using a sheet pan to flip but it still wanted to crack in half and lose a corner. I was reassured it would be okay....but I'm not to worried if I don't see a third flush....any tips or advise from experienced cultivators is welcomed!

Meanwhile, Penis Envy pinned a second flush and they are really really pretty mushrooms! No mutants this time...one or two....but I believe they will get very large! They are already quite big and have not opened their caps which PE does very prematurely! Hoping for a couple monsters to emerge over night and throughout tomorrow as I employ the neglect Tek. At any rate here we go...try to guess which tub is which:


I'm going to go ahead and title this a completed grow, but I will continue posting updates until I know the fate of B+'s third flush! Thanks so much for reading my work! It means the most to me to have all you guys sending my project positive vibes; I whole heartedly believe it greatly lends itself to my success. If you are a new cultivator reading this thread I encourage you to check out my newly published mycology supplies list now linked in my signature below!


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Edited by Mycologist217 (12/12/15 07:13 PM)


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22651118 - 12/13/15 06:10 PM (8 years, 1 month ago)

Day 58-

Monstrous second flush fruits!!


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22653563 - 12/14/15 11:05 AM (8 years, 1 month ago)

Day 59-
Harvesting these giant penises later tonight:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22653868 - 12/14/15 12:33 PM (8 years, 1 month ago)



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22654288 - 12/14/15 02:57 PM (8 years, 1 month ago)

Quote:

Mycologist217 said:
Day 59-
Harvesting these giant penises later tonight:




Hell yea man nice!!!:crazy2::mushroom2:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrTrips31]
    #22654899 - 12/14/15 05:21 PM (8 years, 1 month ago)

Quote:

Mycologist217 said:
Just harvested these fruits and left a lot of them to do some over night work:


:prettyflyforawhiteguy::wintheinternet::breakthrough:




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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22654912 - 12/14/15 05:24 PM (8 years, 1 month ago)

:casket::splooge::


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22655946 - 12/14/15 09:05 PM (8 years, 1 month ago)

Well done! Got me wanting to do some monotubs :smile:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217] * 1
    #22656249 - 12/14/15 10:00 PM (8 years, 1 month ago)

Quote:

Mycologist217 said:
Quote:

Mycologist217 said:
Just harvested these fruits and left a lot of them to do some over night work:


:prettyflyforawhiteguy::wintheinternet::breakthrough:







That's a beautiful thing :amusedapplause::fuckinawesome::dancingshroom:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrSturgill]
    #22659783 - 12/15/15 06:49 PM (8 years, 1 month ago)

Day 60-

Final shots of the second flush monster Penis Envy before harvest!


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22660193 - 12/15/15 08:06 PM (8 years, 1 month ago)

Can't help myself tonight:


Ate a 55 gram fruit.....already getting wavy


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22660287 - 12/15/15 08:24 PM (8 years, 1 month ago)

Quote:

Mycologist217 said:
Can't help myself tonight:


Ate a 55 gram fruit.....already getting wavy



Nice i dont blame you man have a nice trip :bb: iam sitting here looking at a Q of dried PE my buddy sent me took a small dose the other night goin to eat a 3.5 this weekend they are the ones in my sig:mushroom2:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: MrTrips31]
    #22661371 - 12/15/15 11:28 PM (8 years, 1 month ago)

Uh 55 grams fresh of PE.... see you in a few hours OP.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22661382 - 12/15/15 11:33 PM (8 years, 1 month ago)

Quote:

Inocuole said:
Uh 55 grams fresh of PE.... see you in a few hours OP.



haha true that he going to be gone  :letyourspiritsoar:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22661401 - 12/15/15 11:40 PM (8 years, 1 month ago)

Thanks for sharing OP...:thumbup:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: micelio]
    #22661516 - 12/16/15 12:34 AM (8 years, 1 month ago)

Coming back to earth now.....,that was the most radical trip of my life........holy shit


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22661545 - 12/16/15 12:50 AM (8 years, 1 month ago)

Nice log OP, was documented very well.

But only 4 hr trip? :eek: I am confused!


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: RaggedButRight]
    #22661588 - 12/16/15 01:13 AM (8 years, 1 month ago)

Quote:

RaggedButRight said:
Nice log OP, was documented very well.

But only 4 hr trip? :eek: I am confused!




Mycologist217 is a fictitious entity using images supplied by Google to mask his/her inability to form normal social habits.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22661631 - 12/16/15 01:34 AM (8 years, 1 month ago)



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: RaggedButRight]
    #22661715 - 12/16/15 03:09 AM (8 years, 1 month ago)

Uh... okay.  That's not what people usually say after they eat a bunch of PE but hey... whatever floats your scrote.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22662360 - 12/16/15 09:34 AM (8 years, 1 month ago)

Good job man! Dont be hesitant to send me some of those lol:mushroom2:


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22662374 - 12/16/15 09:38 AM (8 years, 1 month ago)

Quote:

Inocuole said:
Uh... okay.  That's not what people usually say after they eat a bunch of PE but hey... whatever floats your scrote.




Are you suggesting that everyone who eats a fresh penis envy mushroom says the same thing when they are coming off their peak?  Can you please tell us all what people DO usually say after the ride of their lives because I thought "holy shit" summed it up nicely.....honestly Inocuole if I wanted to go through this log and quote all the times you've made contributions to it that aren't at all correct and from a totally inexperienced point of view I could have a good time doing it....but really...,I'll leave that to you and the readers. Unless you are wanting to positively contribute by spreading knowledge in this grow log please refrain from posting on it again. Thanks.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22662390 - 12/16/15 09:44 AM (8 years, 1 month ago)

Uh yeah I meant this:

Quote:

Mycologist217 said:
Quote:

RaggedButRight said:
Nice log OP, was documented very well.

But only 4 hr trip? :eek: I am confused!




Mycologist217 is a fictitious entity using images supplied by Google to mask his/her inability to form normal social habits.





And whatever dude, just because when I was giving advice, it was something you didn't care to hear, doesn't invalidate it.  But obviously you're sensitive so I guess I'll take every possible measure to keep from potentially upsetting you. :rolleyes:

But no, please, tell me about my "incorrect contributions" that stem from inexperience or whatever you're accusing me of.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style *DELETED* [Re: Inocuole]
    #22662444 - 12/16/15 10:03 AM (8 years, 1 month ago)

Post deleted by Mycologist217

Reason for deletion: Business settled



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22662453 - 12/16/15 10:06 AM (8 years, 1 month ago)

Man like 10 people told you that tub was ugly.  It was.  It yielded pretty damn well.  But the second flush was in fact diminished was it not?  How are you gonna say shit like "I valued your guess least of all" and then tell me to be cool?  You either want to be cool or you want to do the ol back and forth, which is it?

The PE was super mutated and ugly looking (not necessarily ugly in a bad way).  It got bigger than a lot of people guessed, and that's cool, but usually when my PE looks like yours did I prepare for blob country.  I don't see anything wrong with me warning you of that possibility.  I also don't see a problem with saying it's a little weird to offer the ol "fictitious entity" response after someone asks you about a strong trip.  I mean some people do, but it's usually because they sketched themselves out while tripping and are throwing away all their grow shit.


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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Inocuole]
    #22662482 - 12/16/15 10:20 AM (8 years, 1 month ago)

I Just must not be understanding your temperment then, because since I started my grow log I've felt hostility from you, I reached out and expressed that I'm trying to be a positive member here...lets just move forward agreeing that positivity is the best way to enjoy this hobby! I apologize here for losing my temper.


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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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Offlinetreewitch
psychonaut/jr mycologist


Registered: 08/31/15
Posts: 85
Last seen: 3 years, 5 months
Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22662835 - 12/16/15 11:55 AM (8 years, 1 month ago)

Quote:

Mycologist217 said:
I Just must not be understanding your temperment then, because since I started my grow log I've felt hostility from you, I reached out and expressed that I'm trying to be a positive member here...lets just move forward agreeing that positivity is the best way to enjoy this hobby! I apologize here for losing my temper.




Inocuole seems to rub folks the wrong way wherever he goes. I think it's just his delivery comes off as douchey through text based medium.


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: treewitch]
    #22667200 - 12/17/15 10:52 AM (8 years, 1 month ago)

Day 62-

Flipped b+ back over today cuz I'm stupid......third flush incoming:


--------------------
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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22688587 - 12/22/15 10:07 PM (8 years, 1 month ago)

Day 67-
I harvested the third flush of B+ yesterday. They got to be very large size fruits with some dry caps spanning almost 3". I've had alot going on with the holiday season so I got a little sidetracked from my usual photo stream. So unfortunately I don't have any shots of the maturing third flush. I was so busy or neglectful with it that I let some spores drop.....i decided to go ahead throw the substrate outside after I got tue fruits (almost 3 oz dry) into the dehydrator. Its not exactly freezing where I am so maybe I get some wild Christmas presents later? If i do I will definitely get pictures up.

Meanwhile I've been watering the penis envy trying to coax it into a few third flush fruits....gonna give it another week and call this grow officially closed....pics of dry third flush B+ later. Merry Christmas my friends!


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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OfflinePsilosoulful

Registered: 09/05/14
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22690103 - 12/23/15 11:12 AM (8 years, 1 month ago)

Quote:

Mycologist217 said:
I harvested the third flush of B+ yesterday. They got to be very large size fruits with some dry caps spanning almost 3".



:whoah:


Quote:

Mycologist217 said:
Meanwhile I've been watering the penis envy trying to coax it into a few third flush fruits....



Lol, you mean misting it?
Or are you dunking the entire substrate?


Quote:

Mycologist217 said:
Merry Christmas my friends!



:elmo:


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
    #22695628 - 12/24/15 05:21 PM (8 years, 1 month ago)

Day 69-

Yes of course I meant misting....,misting watering....I don't think there's much of a discrepancy there....

Just few shots of the dry third flush B+  monsters......one of them Being extremely Penis envyish:


The whole fruit in the middle pic the PE like one....not a large fruit but that guy masses to 3.3 grams....


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineMycologist217
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22700811 - 12/26/15 07:21 AM (8 years, 1 month ago)

Quote:

Mycologist217 said:
I threw my B+ substrate outside last week after I harvested the third flush. Check it out yesterday (Christmas) it's covered in new pins:





--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineJabensis
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
    #22700933 - 12/26/15 08:39 AM (8 years, 1 month ago)

Don't you love it when an old sub still wants to put out, despite being put-out.  HA

I just spent my morning reading this whole grow, and man, I gotta say, great job.  I wanted to see what I can expect for my first cased, PE mono.  Thanks for all the pics!  It really helps to compare.

p.s. the deer, chipmunks, and squirrels in my area love to tear apart spent subs.  Also, for future reference, the microclimate under a shrub or bush or even a lean-to (head room) of fallen sticks and brush is a great fruiting environment for spent subs.


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Offlinepig5
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Registered: 12/30/15
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Last seen: 7 years, 4 months
Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Jabensis]
    #22758198 - 01/09/16 05:28 PM (8 years, 21 days ago)

nice


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OfflineFractal420
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: spacechildo]
    #23047331 - 03/26/16 07:43 AM (7 years, 10 months ago)

I zoomed in and a batman joker poster literally made me jump. Like i am just looking for the supposed bacteria growth up close, and i look to the side and POP UP FACE ahhh. Lol!

I dont see anything i recognize as bacteria but its hard to tell. I feel like usually if theres bacteria, there are usually metabolite drops around the jar (before it is fully colonized), or theres weird cauliflower style growths. The advice of shaking the jar is a great idea because it lets you see clearly whether or not your grains are clean.


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Dreaming of That face again.
It's bright and blue and shimmering.
Grinning wide
And comforting me with it's three warm and wild eyes.

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