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Psilosoulful

Registered: 09/05/14
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22532017 - 11/16/15 10:49 AM (8 years, 2 months ago) |
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The casing looks good, you just want a nice thin layer over the surface, no need to break out the ruler or anything 
And as long as your pasteurization procedures were good, you don't have to worry about it contaminating.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
#22537482 - 11/17/15 12:56 PM (8 years, 2 months ago) |
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Day 32-
So now we are coming down to the waiting game....the first time I did a monotub it took like 16 days to start pinning after fruiting conditions were introduced, but i did not case that. I would imagine casing slows down pinning since the peat and verm will get somewhat colonized?
I am very curious to see the difference casing Penis Envy tub makes verses not casing it. When I did not case it I had a 2.5 oz first flush about....I know genetics obviously play a huge role in fruiting behavior but I want to imagine the environment created by the lime and peat and verm will encourage extremely widespread pinning.
I am also eager to clone a fruit to agar since my MS spore inoculations are not going very well at all.
Pictures: Penis Envy (cased) on left, B+ (not cased) on right.
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22540991 - 11/18/15 09:14 AM (8 years, 2 months ago) |
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Day 33-
Just a couple days into fruiting now. The PE mycelium is starting to make work of the casing layer and I would imagine that if I cased then continued colonizing before fruiting then I would be fruiting this morning anyway.....usually I'm pretty good about curbing my anxiety waiting on pins but right now I am very eager to see it happen. The results displayed by people using the jiffy mix to case their PE are so astounding. I know I inoculated from a MS spore syringe but THE source has never provided me with weak genetics. I would guess spore syringes are made from fruits with decent genetics by practice. At least I have an organic chemistry and cell bio final to study for this week.
Pics:

Pink for PE. blue for B+
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22545867 - 11/19/15 09:31 AM (8 years, 2 months ago) |
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Day 34- I think the PE tub is starting to knot. The B+ looks less colonized today than it did yesterday....weird...see pics then compare to see what I mean....I missed the B+ tub last night so maybe it just looks different because more moisture? Not really worried about it I suppose...
Let me know what you think of things so far, PE is the cased tub and B+ is not cased:
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Edited by Mycologist217 (11/19/15 09:36 AM)
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TriggerFish
Stranger

Registered: 09/23/15
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22546689 - 11/19/15 12:51 PM (8 years, 2 months ago) |
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Eagerly awaiting results.
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: TriggerFish]
#22549492 - 11/19/15 10:44 PM (8 years, 2 months ago) |
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So the PE is just tearing away at that casing layer....very evenly across the whole tub...do I need to do anything? Experienced cultivators with PE on WBS to coir/verm/gypsum cased with jiffy mix.

PE is left two photos. Right two are just pic update of B+
Once again to experienced cultivators, do I need to do anything like "patch" that casing?
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22550699 - 11/20/15 09:26 AM (8 years, 2 months ago) |
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Day 35- Just a picture update and the sa,e question as last night:
Penis Envy, do I need to patch this up or anything or just let it be itself? First time casing so I don't know:

B+ photo update:
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22551180 - 11/20/15 11:25 AM (8 years, 2 months ago) |
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Quote:
Mycologist217 said: Day 35- Just a picture update and the sa,e question as last night:
Penis Envy, do I need to patch this up or anything or just let it be itself? First time casing so I don't know:

B+ photo update:

I believe at 20?% casing colonization you introduce to fruiting? At least it was what I did and had a nice flush
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: GreenHornThumb]
#22553263 - 11/20/15 09:51 PM (8 years, 2 months ago) |
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Oh man I am getting obsessed with this PE tub....I am so happy my university lets out the whole week for holidays! i wonder if I will ever get so involved cultivating that I don't even notice pinning times anymore, but right now it's almost unbearable....like its my first grow all over again....but I feel like my tub looks great....it looks awesome...and I hope it fruits just as amazingly....I a, worried the B+ tub will know I don't care as much about it so I try to include it as much as I can, but really my mind is on this cased PE tub during all my waking hours...
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22557000 - 11/21/15 07:22 PM (8 years, 2 months ago) |
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Day 36-
The B+ tub is pinning!!!!!    Ironically it has pinned on day 36 where the penis envy monotub I grew in April also pinned on day 36! Weird...especially as these jars had a full ten days longer in colonization, but oh well...
There are dozens of extremely small very newly debuted pins and they are impossible to see from a far so I got a shitty picture up close....I don't know why the iPad's camera is so shitty....probably so they can upgrade the next model...when I had an iPhone the pictures were way way better....

The PE tub is doing some serious work on the casing layer but it is starting to know up really nicely and I think it will be just a couple days (more like hopeful) behind its brother B+
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22557353 - 11/21/15 08:47 PM (8 years, 2 months ago) |
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
#22561248 - 11/22/15 08:18 PM (8 years, 2 months ago) |
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Day 37- Obviously things are very exciting right now with the B+ tub. A bunch of new pins appeared today and I am expecting more and ,ore over the next couple days. I gave the tub a 180Degree spin as the pins seemed concentrated toward one end of the tub. Hope that's a good play..,,the pins that were there last night were a little more visible tonight with only one "cluster" being present.

Hopefully I end up getting decent mass in the first flush, but I understand ms inoculation is a crapshoot
Penin Envy tub is knotting up like crazy and the casing layer almost fully colonized....ready for a great flush (HIGH HOPES )
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22564966 - 11/23/15 07:07 PM (8 years, 2 months ago) |
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Day 38- I really need some advice on how to feel about this. The penis envy tub is glittered with yellow metabolites...I don't know what it means or what to do about it as this is my first encounter. Please take a look and let me know what's up please:

In other news B+ is starting to pin more and more:

Again please help me out on the PE tub.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22570465 - 11/24/15 09:02 PM (8 years, 2 months ago) |
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Day 39- Tonight I'm okay I guess...I've been searching and searching through the forums trying to find out what's up with these yellow drops of water....most of what I've found the grows turned out fine....the substrate is knotted like crazy.......I am just praying with all my possible high hopes that it turns out okay....forty days to run into problems now isn't cool, but I'm cheerful I suppose. Increasing air flow instantly resulted in more primordia so we'll see how next couple days go:

B+ is pinning more and more buts not exactly the wonderland full canopy I was hoping more....I guess the next couple days will also a lot about the B+ tub...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22570745 - 11/24/15 10:10 PM (8 years, 2 months ago) |
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whats your substrate?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: cronicr]
#22570753 - 11/24/15 10:12 PM (8 years, 2 months ago) |
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Quote:
cronicr said: whats your substrate?
WBS spawned to coir/verm/gypsum cased with jiffy mix containing 50-60 peat moss to verm and some lime
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22570763 - 11/24/15 10:15 PM (8 years, 2 months ago) |
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nice i wouldn't sweat it man just keep doing what you are doing because you're doing great, metabs can be from lots of shit from bacteria to an over colonized sub to temps to absolutely nothing more then it eating
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mycologist217
Frank's Disciple



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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: cronicr]
#22574787 - 11/25/15 07:01 PM (8 years, 2 months ago) |
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Day 40- Something about the way Cronicr compliments a guy really calmed me down. I am seriously waiting on baited breath for the PE tub to pin and I am grateful that the B+ is doing work.
I am happy that more and more pins keep popping up in the B+ tub..lif every single primordia became a fruiting body I would be in extasy. There are lots and lots of pins on the side of the tub and the middle is filling out, favoring one end of the tub but the other end has a few...I a, curious to see how long it forms pins for before they are all just growing fruits with no new ones forming...I see people's pictures of hundreds of pins coming up together....obviously this is not going to be what happens here...or could it still happen?
Keep sending good vibes to the PE tub:

I plan using a larger fruit from the cluster on the the right picture to take a tissue transfer (clone) from. Is this the type of formation I want to be taking samples from?
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Edited by Mycologist217 (11/25/15 07:03 PM)
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Psilosoulful

Registered: 09/05/14
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Mycologist217]
#22575323 - 11/25/15 09:17 PM (8 years, 2 months ago) |
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Quote:
Mycologist217 said: I plan using a larger fruit from the cluster on the the right picture to take a tissue transfer (clone) from. Is this the type of formation I want to be taking samples from?
Yes, that'll be fine. Take a clone of the fastest/largest growing mushie
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: Grow Log- WBS- Penis Envy and B+ Monotubs -Lab Manual Style [Re: Psilosoulful]
#22579111 - 11/26/15 04:57 PM (8 years, 2 months ago) |
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Day 41- Tons and tons of B+ pins....a couple that are clearly going to abort....and a few looking like they need more FAE....but there's lots of them...

In other news....THE PENIS ENVY TUB IS BLOBBING AND PINNING!!!!! No real pins yet, but I am sure a couple are there....several freckled blobs made their way up though....teeny tiny...they remind me of birds eggs for some reason....sure we'll get more and more in the next couple days but hopefully we get lots and lots of pins....little dick shaped mushroom pins.
So I want to take a couple seconds to talk about my grow room....give some insight to the newer cultivators here, so first....picture:
 
You'll notice that in the lower left hand corner [of the room] is a room fan. I had it on medium until I got all these metabolite pools on my PE tub a few days back. I turned it to high to increase FAE. You'll see the fan is pointed into the wall and that the tubs are positioned to where the long ends form a right angle to direction of the fan....then in the next photo you'll see a completed unshuttered window that's open. This window has been open since fruiting started as a means to maintain FAE and not just move stale air around the room. The temperature in this rooms hovers around 70-74F....seeing good results so far fruiting stuff in this room.
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Edited by Mycologist217 (11/26/15 05:07 PM)
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