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OfflineNosceteipsum
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Colonization not happening. Jar, temp, sub, spores?
    #22381090 - 10/14/15 09:55 PM (8 years, 3 months ago)

Hello, fellas. I'm a newbie trying to figure out why my jars aren't colonizing. I inoculated some PC spores over a week ago in 7 quart-sized jars with over 1/4 part of brown rice grain and 1/2 part of vermiculite, and some water of course. The jars should be out of contaminants since I was very careful with that issue (anyway a noob could make a mistake).

I'm wondering if the jars have anything to do with it, or if it could be the substrate (maybe too much vermiculite and very little rice?). Those are the principal reasons why I imagine it isn't colonizing, but could be also temperature or sh*it-spores (I bought 'em from "a friend of a friend"). Anyway, I'd like to know if you could shed some light over the jars/substrate thing. Feel free to ask any questions and thank you in advance :smile:


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Offlinetetherface
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: Nosceteipsum]
    #22381145 - 10/14/15 10:09 PM (8 years, 3 months ago)

Did you follow a tek that said to use quart sized jars? Is there a dry verm layer on top? What did you use fir a lid??


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OfflineNosceteipsum
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: tetherface]
    #22381894 - 10/15/15 04:01 AM (8 years, 3 months ago)

Hi, tetherface. No, I used a conventional 1/2 pint jar tek and adapted it to my quart-sized jars; I didn't use a dry verm layer on top, but I use the actual jars' lids with a layer of aluminuum foil held with a rubber band on top. Thanks for your reply :smile:


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OfflineJaboka
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: Nosceteipsum]
    #22381948 - 10/15/15 04:48 AM (8 years, 3 months ago)

I dont think its from the jars. I bet on the spores. I've seen a lot of threads where due to spores from not certified seller, colonization is going extremely slow, or its doesn't start at all.


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Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


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OfflineJaboka
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: Jaboka]
    #22381950 - 10/15/15 04:49 AM (8 years, 3 months ago)

Tomorrow I am inoculating 1/2 jars as well with rye only. I will let you know about how my colonization goes in these jars, but once again it thinks their a not the problem. Good luck.


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Hunters with their dogs and deer rifles; thousands of them line the pavement; like patient pupae waiting to become worms.


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OfflineNosceteipsum
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: Jaboka]
    #22385313 - 10/15/15 08:02 PM (8 years, 3 months ago)

Yup, I'm starting to think of that, but I still have some hope for a few reasons it's not worth detailing here.

Anyway, what do you think about temp? I live in a really cold place -even though it's spring- and the temperature goes from 10°C to 23°C (idk what that'd be in Farenheit) during the day, and lowers down to 0°C - 10 °C at night. I have my jars in a plastic monotube, approximately 1 1/2 meters above a small heater. Describing it like that somehow makes me feel like I'm missing some care in the temp issue.

Last question: supposing that the spores are actually spores and that the problem is actually temp. and not anything else, how long would it take spores to lose the possibility of colonization? I mean, is it possible that I can save them by putting them in the right ambient?

Thank you very much for you answers and time :smile:


Edited by Nosceteipsum (10/15/15 08:03 PM)


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Offlinetetherface
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: Nosceteipsum]
    #22385362 - 10/15/15 08:13 PM (8 years, 3 months ago)

Spores can take 2 weeks to germinate what concerns me is that you didnt modify your lids any way to allow GE which may/probably will cause them to stall later on.How full did you make your jars? Im assuming you plan on fruiting them invitro since you wont be able to remove the cake? The other question I have is did you use brown rice flour? Cause in the OP its not really clear the biggest thing you need yo do is follow a tek exactly as its writtenat least until you get a good feel for the hobby


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OfflineNosceteipsum
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: tetherface]
    #22385464 - 10/15/15 08:31 PM (8 years, 3 months ago)

The thing is, I didn't exactly make cakes; I used brown rice in its grain form (boiled previously) and vermiculite so it could be possible to crumble and spawn it to some horse manure. As with how full did I make the jars... well, I filled them completely but without pressing the substrate in them, then I shook them a little bit to add some more sub., but again, without pressing, so my jars have a nice quantity of air in them. I would seriously like to know more about GE, if you think that could help me.

Thank you again :smile:


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Re: Colonization not happening. Jar, temp, sub, spores? [Re: Nosceteipsum]
    #22385651 - 10/15/15 09:06 PM (8 years, 3 months ago)

Is there any way you can post pics of these I've never heard/seen anyone mix WBR and verm you can use the WBR by itself and succeed but frankensteining a grow together is a sure way to fail especially at the very beginning of your myco-career. To be honest i would start over or at least start another round of jars and wait and see what happens with the qts. How did you inoculate the jars if you didn't modify the lids? And did you use a SAB ? And how did you sterilize these jars?


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OfflineNosceteipsum
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: tetherface]
    #22385794 - 10/15/15 09:29 PM (8 years, 3 months ago)

You think that could be the issue? I'll make another batch with only WBR to see what happens, luckily I have 3 more syringes :smile:. I didn't think mixing the rice with verm could be harmful. I can't take any pics right now since I lost my cellphone and have no camera available, but I'll try to later. What I did with my jars was to make four holes on each lid for posterior inoculation then added a couple of aluminuum foil layers held with rubber bands; after that I sterilized them in a PC for about and hour. I let them cool and prepared my bathroom (completely cleaned it and disinfected it) to inoculate them there. For inoculation I took the aluminuum foil covers and after inoc. I put one cover back on each jar.

Thanks for your advice, tetherface, I'll make some more jars with new sub and see what happens. :smile:


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Re: Colonization not happening. Jar, temp, sub, spores? [Re: Nosceteipsum]
    #22385854 - 10/15/15 09:45 PM (8 years, 3 months ago)

That's probably part of it you need to modify your lids to allow for GE thru some sort of filter material just search around till you find something you like i use ez felt siliconed over a small hole like this other than that follow an actual tek to the T here's a good link for the WBR prep https://www.shroomery.org/forums/showflat.php/Number/21126568#21126568 so do a bit of reading fix your lids and get back on track:nyan:


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OfflineNosceteipsum
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Re: Colonization not happening. Jar, temp, sub, spores? [Re: tetherface]
    #22385903 - 10/15/15 09:56 PM (8 years, 3 months ago)

I'll do that, thanks! :smile:


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