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OfflinecronicrFacebook
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Cloning Wild Fruits * 12
    #22377031 - 10/14/15 04:56 AM (8 years, 3 months ago)

Going to doc a couple ways I like to do this, from typical cloning to wild fruits to dried fruits.
To start this I got my sterile water ready, two small ones have a wee bit of bleach
.
From there I will either take the two small bottles with me in the field or just use them at home but they are my starting point.

When in the field I simply cut the cap off after pulling the mushroom from the ground, I then wipe any debris off the base  and will drop the tissue in the water and shake, this rinses off a lot of contams and the bleach helps as well.

The next step is removing it once home and putting it in another jar of sterile water and shaking a few times, this step can be repeated a couple times and this greatly waters down any contams present and gives your tissue sample a much better chance and without things like Iso and iodine which may make things harder!

So we took some gyms and did steps one and two and proceeded to clone like normal taking the last sterile water jar with the tissue sample to the sab for final removal before cloning to no pour Petri's with premixed mea.

After wiping down the water jars and agar jars in iso and prepping the sab like normal I took the tissue sample out from the water and tore it in half from the top down and put three samples in three agar jars.
Within 3 days I had great growth in them and was ready for the first transfer in just four days.



A few transfers in....

.

ready for grain

for the first run though i'm going to go to supplemented jars(wheat gypsum coffee bran wodchips)
\
Here i did this with an Agaricus mushroom.

Cut the stipe

Add to jar 1 and shake

Transfer to jar 2 and shake and repeat for about 3 jars of water/bleach

Then take it out and place it in your prepped sab.

Tare it from the top down in half

Take your tissue sample from the inner base

Place on agar wait for growth.

Here's a quick over view on how i did the dry fruit clone......no different from what you see above otherthen the first soak sits for a bit

dried fruit


and gold




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OfflinePsilosopherr
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Re: Cloning wild fruits [Re: cronicr]
    #22447505 - 10/29/15 12:53 AM (8 years, 3 months ago)

:popcorn: First! :crondance:


Edited by Psilosopherr (09/09/16 08:07 PM)


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OfflinecronicrFacebook
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22507985 - 11/11/15 04:37 AM (8 years, 2 months ago)

This thread was moved from the user's journal.

Reason:
Why must I always have a reason:smbfacepalm:


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Invisibleoontribe
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22508017 - 11/11/15 05:20 AM (8 years, 2 months ago)

thanks for sharing man :smile:


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Invisiblewowimflabbergasted
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Re: Cloning Wild Fruits (moved) [Re: oontribe]
    #22508222 - 11/11/15 07:41 AM (8 years, 2 months ago)

Sweet nice pic tutorial cron!


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Offlinespacechildo
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Re: Cloning Wild Fruits (moved) [Re: wowimflabbergasted]
    #22508774 - 11/11/15 10:43 AM (8 years, 2 months ago)

aaaw yeah, here we go!


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Re: Cloning Wild Fruits (moved) [Re: spacechildo]
    #22508838 - 11/11/15 11:01 AM (8 years, 2 months ago)

Just making sure I can find this again.


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Invisible36fuckin5
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Re: Cloning Wild Fruits (moved) [Re: thebug76] * 1
    #22509356 - 11/11/15 01:27 PM (8 years, 2 months ago)

But you forgot the part that makes it hard...

Oh wait, that isn't a step. It's really this easy, folks.


--------------------
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OfflineDarkhome
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Re: Cloning Wild Fruits (moved) [Re: 36fuckin5]
    #22509406 - 11/11/15 01:40 PM (8 years, 2 months ago)

:takingnotes:Good info! Thanx!


--------------------
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OfflineSivarted
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Re: Cloning Wild Fruits (moved) [Re: Darkhome]
    #22509546 - 11/11/15 02:12 PM (8 years, 2 months ago)

I've seen a couple different posts about cloning wild mushrooms, and I'm having trouble seeing why cloning a wild one would be any different than cloning one from an indoor grow.  Yeah, maybe outside is dirtier and more contaminated (although after accidentally seeing the some of the shit that is more than happy to grow on agar in my bedroom, I might challenge that statement), but if the steps for cloning a promising mushroom from your grow (or really, for anything involving contams, ive had to follow this process to get something out of a horribly contaminated syringe) are:

Step 1: Get a bit of interior tissue onto agar
Step 2: transfer away from contams, repeat until you have a clean culture

Then why do wild mushrooms require special treatment?

I'm not being at all dismissive here, this is an honest question, because I'm probably going to hunt edible species in my area at some point.


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Re: Cloning Wild Fruits (moved) [Re: Sivarted]
    #22509587 - 11/11/15 02:21 PM (8 years, 2 months ago)

sweeet. :thumbup:

faht


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22509601 - 11/11/15 02:24 PM (8 years, 2 months ago)

Quote:

cronicr said:
<img src='https://www.shroomery.org/forums/images/moved.gif'> This thread was moved from the user's journal.

Reason:
Why must I always have a reason:smbfacepalm:




:lolsy: sounds like some first world problems. Perhaps :rockon: will suffice.


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Offlineinvitro

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Re: Cloning Wild Fruits [Re: cronicr]
    #22509611 - 11/11/15 02:28 PM (8 years, 2 months ago)

:thatsinteresting:


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Re: Cloning Wild Fruits (moved) [Re: Sivarted]
    #22509754 - 11/11/15 03:08 PM (8 years, 2 months ago)

:lol:
Quote:

Sivarted said:
I've seen a couple different posts about cloning wild mushrooms, and I'm having trouble seeing why cloning a wild one would be any different than cloning one from an indoor grow.  Yeah, maybe outside is dirtier and more contaminated (although after accidentally seeing the some of the shit that is more than happy to grow on agar in my bedroom, I might challenge that statement), but if the steps for cloning a promising mushroom from your grow (or really, for anything involving contams, ive had to follow this process to get something out of a horribly contaminated syringe) are:

Step 1: Get a bit of interior tissue onto agar
Step 2: transfer away from contams, repeat until you have a clean culture

Then why do wild mushrooms require special treatment?

I'm not being at all dismissive here, this is an honest question, because I'm probably going to hunt edible species in my area at some point.



Outdoors is strait up dirty! i can't remember the numbers exactly but trust me it's friggiun insane how many contams can be found inside and outside of a fruit:crazy:


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OfflineMycologist217
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22509758 - 11/11/15 03:10 PM (8 years, 2 months ago)

You da man Cronicr!


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Re: Cloning Wild Fruits (moved) [Re: Mycologist217]
    #22509778 - 11/11/15 03:13 PM (8 years, 2 months ago)

found a sweet patch across the street from me of some edibles i'm going to dive in on tonight, i'll get more pics up of the ones i did already there is great looking growth!


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleAdden
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22509850 - 11/11/15 03:27 PM (8 years, 2 months ago)

:threadmonitor:


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OfflineMoldy thumb
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Re: Cloning Wild Fruits (moved) [Re: Adden] * 1
    #22509921 - 11/11/15 03:44 PM (8 years, 2 months ago)

:takingnotes:


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InvisibleAdden
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Re: Cloning Wild Fruits (moved) [Re: Moldy thumb]
    #22509933 - 11/11/15 03:45 PM (8 years, 2 months ago)

Haha, yep!


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: Adden]
    #22526970 - 11/15/15 10:21 AM (8 years, 2 months ago)

So, do I sterilize a container and then poor the sterile water into the said sterile container, and let it cool few a few hours before applying the mushroom to the water? Or do I sterilize the water using a cap of bleach and just toss the mycelium into it?


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OfflinecronicrFacebook
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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22527652 - 11/15/15 02:09 PM (8 years, 2 months ago)

i put water in a jar and pc it


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Offlineinvitro

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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22527687 - 11/15/15 02:23 PM (8 years, 2 months ago)

Is "wee bit"  just a few drops of bleach?


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22527933 - 11/15/15 03:09 PM (8 years, 2 months ago)

I rinsed with a water filtered through a brita pitcher after doing that bleach/water rinse. Guess we'll see what happens :shrug:


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22527948 - 11/15/15 03:13 PM (8 years, 2 months ago)

nothing wrong with that, that water is cleaner then what is on/in the fruit by far. good luck man! i'm cloning a clitocybe nuda right now from my back yard


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22527970 - 11/15/15 03:18 PM (8 years, 2 months ago)

:cheers:
I wanted to clone a cool mushroom from my back yard. Smells great, but could be poisonous. They're related to portobellos


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22527982 - 11/15/15 03:21 PM (8 years, 2 months ago)

ya might try find something fresher to clone lol! Good luck though i would give it a shot anyway just for the practice, if i get any clean growth from my agaricus i'll hit ya up.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22527986 - 11/15/15 03:23 PM (8 years, 2 months ago)

Yeah? I got a print, but I think agar is the way to go. Just gotta get more practice. Think they'll grow okay on a standard pf tek?


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22528098 - 11/15/15 03:59 PM (8 years, 2 months ago)

i believe agaricus species like compost, not certain on all species though but indeed they will colonize a cake.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22528118 - 11/15/15 04:06 PM (8 years, 2 months ago)

:borat:

This is the first year that I've seen this species around here. Same with that chicken of the forest of whatever it's called. Maybe I simply wasn't paying enough attention.


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22528121 - 11/15/15 04:07 PM (8 years, 2 months ago)

I hear ya, i don't' pay much attention to city dwelling mushrooms till this past couple of season, it's chicken of the woods btw:lol:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22528125 - 11/15/15 04:09 PM (8 years, 2 months ago)

Close :lol:


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22536347 - 11/17/15 07:56 AM (8 years, 2 months ago)

I've got two jars I wanna do invitro. They're fully colonized but smell a tad bit bacterial. Should I birth them into a bleach water rinse and then clean the jars, and put them back into the jars with a vermiculite casing layer on top?


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22536736 - 11/17/15 10:09 AM (8 years, 2 months ago)

no bacteria is not much of a threat to a fruiting sub, it can defend itself against it at this point just make sure to give it some air if possible


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22536742 - 11/17/15 10:10 AM (8 years, 2 months ago)

Fair enough :cheers:


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22541283 - 11/18/15 10:25 AM (8 years, 2 months ago)

I've got some PE6 that stalled in the jars on my second failed growing attempt. I buried them in my cow poo pile and now they're fruiting. I picked a couple that weren't quite open yet. I think I'm going to try cutting the veil with a sterile knife to get a clean spore syringe, and also try this tek with some tissue culture. I'll have to try an LC instead of agar (no PC). Keeping my fingers crossed and I'll let you all know how my attempt goes.


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: thebug76]
    #22541290 - 11/18/15 10:28 AM (8 years, 2 months ago)

If I get a clean transfer from agar without a PC, do I get a cookie?
:cookiemonster:


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22541297 - 11/18/15 10:32 AM (8 years, 2 months ago)

Lol, if you can teach me to do it without a PC, I'll have my wife make you a whole batch of cookies.


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: thebug76]
    #22541303 - 11/18/15 10:35 AM (8 years, 2 months ago)

I'll do a write-up after I get the clean transfer; Don't wanna count my eggs before they hatch or something like that. :pipesmoke:


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Invisible36fuckin5
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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22541310 - 11/18/15 10:38 AM (8 years, 2 months ago)

You may get away with agar with no PC a few times but it'll never be a reliable method.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlinespacechildo
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Re: Cloning Wild Fruits (moved) [Re: 36fuckin5]
    #22541321 - 11/18/15 10:42 AM (8 years, 2 months ago)

couldn't you just mix water,brf and agar powder and steam in a pot for a couple hrs?


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: 36fuckin5]
    #22541324 - 11/18/15 10:43 AM (8 years, 2 months ago)

I'll quote you on that in the future :super:


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Re: Cloning Wild Fruits (moved) [Re: spacechildo]
    #22543001 - 11/18/15 05:32 PM (8 years, 2 months ago)

Quote:

spacechildo said:
couldn't you just mix water,brf and agar powder and steam in a pot for a couple hrs?



Yep


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22543008 - 11/18/15 05:33 PM (8 years, 2 months ago)

The real issue with it is carmelizing the agar


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22543028 - 11/18/15 05:39 PM (8 years, 2 months ago)

How do you mean?


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22543118 - 11/18/15 06:05 PM (8 years, 2 months ago)

Sugars tend to carmelize with long term heat, when I did it my agar was very dark


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22543269 - 11/18/15 06:45 PM (8 years, 2 months ago)

Ah, mines dark from the side by transparent from the top/bottom :shrug:


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Offlineinvitro


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Re: Cloning Wild Fruits [Re: cronicr]
    #22546519 - 11/19/15 12:10 PM (8 years, 2 months ago)

Does it hurt anything to keep the stem in the water for an extended period of time?


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OfflineYaMoonSun
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Re: Cloning Wild Fruits [Re: invitro]
    #22546538 - 11/19/15 12:13 PM (8 years, 2 months ago)

Got pink mold in my one of my unbirthed jars; Considering that it's from bacteria (according to an article I read anyways), think that a bleach water bath would off it? This house is so fucking gross :facepalm3:


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OfflineYaMoonSun
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Re: Cloning Wild Fruits [Re: YaMoonSun]
    #22546675 - 11/19/15 12:48 PM (8 years, 2 months ago)

Well, I'm soaking it in bleach water regardless. The pink appears to have faded significantly in moments. I'll just fruit it outdoors or something.


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Re: Cloning Wild Fruits [Re: invitro]
    #22547362 - 11/19/15 03:34 PM (8 years, 2 months ago)

Quote:

invitro said:
Does it hurt anything to keep the stem in the water for an extended period of time?



if ya used bleach then no not really.
gl with that yamoon


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Re: Cloning Wild Fruits [Re: cronicr]
    #22547743 - 11/19/15 04:51 PM (8 years, 2 months ago)

first transfers of the clitocybes in the morning,took 3 samples all 3 were clean! also got the agaricus to do as well,gyms are going into fruiting tonight


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Re: Cloning Wild Fruits [Re: cronicr]
    #22547781 - 11/19/15 04:57 PM (8 years, 2 months ago)

:jackiechanofapproval:


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Re: Cloning Wild Fruits [Re: cronicr]
    #22547783 - 11/19/15 04:57 PM (8 years, 2 months ago)

.


Edited by Mandarinfish (07/15/20 11:00 PM)


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Re: Cloning Wild Fruits [Re: Mandarinfish]
    #22547861 - 11/19/15 05:12 PM (8 years, 2 months ago)

lol no kidding


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Re: Cloning Wild Fruits [Re: cronicr]
    #22558895 - 11/22/15 10:05 AM (8 years, 2 months ago)

I wonder if instead of trying to boil this bacterial syringe, I can just fill it with bleach water, and leave it full until I'm ready fill said syringe with LC, and/or skip flame sterilization in between agar transfers and just have a shot-glass full of bleach/water in the SAB to dip the syringe in in-between transfers. :shrug:


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Re: Cloning Wild Fruits [Re: YaMoonSun]
    #22616495 - 12/05/15 10:59 AM (8 years, 1 month ago)

Well, today's the day, wish me luck.





Edited by YaMoonSun (12/05/15 12:59 PM)


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Re: Cloning Wild Fruits [Re: YaMoonSun]
    #22617345 - 12/05/15 03:26 PM (8 years, 1 month ago)

:fuckinawesome:
same here, knocked up a few more jars cased a couple more and have knots on the ones i did last week


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Re: Cloning Wild Fruits [Re: cronicr]
    #22617486 - 12/05/15 04:01 PM (8 years, 1 month ago)

:borat:
I inspire ya?
I just made a big ol brf jar with high hopes for this LC


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Re: Cloning Wild Fruits [Re: YaMoonSun]
    #22667423 - 12/17/15 11:51 AM (8 years, 1 month ago)

Well, you know my LC went to shit already, but how did your bleach-sterilized syringe tub play out?


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Re: Cloning Wild Fruits [Re: YaMoonSun]
    #22667439 - 12/17/15 11:55 AM (8 years, 1 month ago)

the gym's are doing just fine! i'll have my cam back tonight but so far my gyms agaricus and nuda are all doing just great


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I'm tired do me a favor


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Re: Cloning Wild Fruits [Re: cronicr] * 1
    #22667462 - 12/17/15 12:03 PM (8 years, 1 month ago)

Well that's good to hear :cheers:


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Re: Cloning Wild Fruits [Re: YaMoonSun]
    #22667477 - 12/17/15 12:07 PM (8 years, 1 month ago)

also a fuckton of mushrooms popping up here again so i may do a lib/pellie grow here, we will see in three days


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Re: Cloning Wild Fruits [Re: cronicr]
    #22667543 - 12/17/15 12:24 PM (8 years, 1 month ago)

Sounds like a lot of fun; What sort of substrate did you have in mind for the libs?


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Re: Cloning Wild Fruits [Re: YaMoonSun]
    #22667546 - 12/17/15 12:25 PM (8 years, 1 month ago)

i got no idea yet, both will be for outdoors anyway like most of the psilocybes i have on hand


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I'm tired do me a favor


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Re: Cloning Wild Fruits [Re: cronicr]
    #22667609 - 12/17/15 12:47 PM (8 years, 1 month ago)

I've always wanted to do an outdoor patch; Sounds challenging though.


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22722886 - 12/31/15 11:05 PM (8 years, 30 days ago)

Might not be exactly relevant but I got a dry fruit question:

Got a dry cube with a dark red cap from a friend.  It was harvested before the veil even began to stretch, when it still has that roughled colar look.  Can I crack the cap open and pull some spores with a swab?

Would it be preferable to put the spores into a syringe to rehydrate, rather than swab directly to agar?

It appears to have been grown on a cake, so I'd rather start with vigorous MS growth than a tired clone of a random ms fruit.  I'll try both methods though.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Cloning Wild Fruits (moved) [Re: Machiavelliavore]
    #22722890 - 12/31/15 11:07 PM (8 years, 30 days ago)

you can try to swab it for sure, if there are mature spores it will work fine, no need to rehydrate agar is great at thatbas well.


--------------------

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I'm tired do me a favor


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22722925 - 12/31/15 11:17 PM (8 years, 30 days ago)

I hadn't even considered spore maturation.  I assume they would be mostly mature.  I'd place this mushroom's age as about 1-1.5 days before veilpop.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Cloning Wild Fruits (moved) [Re: Machiavelliavore]
    #22722965 - 12/31/15 11:31 PM (8 years, 30 days ago)

Damn I wish we had wild actives or edibles for that matter here. Sounds like a lotta fun.


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Re: Cloning Wild Fruits (moved) [Re: Supalemonhaze]
    #22723101 - 01/01/16 12:28 AM (8 years, 30 days ago)

my gym clones are pinning on mea.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22723211 - 01/01/16 01:10 AM (8 years, 30 days ago)

Have you eaten any gyms yet?  That's an experience I've been curious to have.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Cloning Wild Fruits (moved) [Re: Machiavelliavore]
    #22723236 - 01/01/16 01:23 AM (8 years, 30 days ago)

a few types, i liked blg and luteoviridis, bitter taste but very clean high.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22797346 - 01/18/16 11:43 PM (8 years, 12 days ago)

This thread was moved from Mushroom Cultivation.

Reason:
Here we go asking for reasons again:rofl:


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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22797899 - 01/19/16 07:19 AM (8 years, 12 days ago)

Someone moved your thread? I thought it was always in mushroom cultivation :shrug:


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #22798168 - 01/19/16 09:06 AM (8 years, 12 days ago)

I moved it,welcome to the amu forum.


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22802480 - 01/20/16 09:27 AM (8 years, 11 days ago)

:popcorn:


--------------------
LAGM v 2.024
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Re: Cloning Wild Fruits (moved) [Re: cronicr] * 1
    #23630196 - 09/09/16 06:41 PM (7 years, 4 months ago)

This thread was moved from AMU.

Reason:
:smile:


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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #23630237 - 09/09/16 06:56 PM (7 years, 4 months ago)

Subbed again.. I'll be trying this on wood agar this season for the F of it.


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Re: Cloning Wild Fruits [Re: cronicr]
    #24642678 - 09/18/17 11:40 PM (6 years, 4 months ago)

Well that time of year...gym beds are knotting up i can't wait!


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I'm tired do me a favor


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OfflineELEVATI0N
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Re: Cloning Wild Fruits [Re: cronicr]
    #25361287 - 08/02/18 12:10 AM (5 years, 5 months ago)

Thank you!


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Re: Cloning Wild Fruits [Re: ELEVATI0N]
    #25635407 - 11/23/18 11:29 PM (5 years, 2 months ago)

Awesome, thanks so much for the great documentation you’ve provided. Wonder if you always take stipes rather than caps when doing tissue transfer?


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Re: Cloning Wild Fruits [Re: michaelstipe]
    #25636670 - 11/24/18 04:11 PM (5 years, 2 months ago)

Most times but the cap works well too


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I'm tired do me a favor


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Re: Cloning Wild Fruits [Re: cronicr]
    #25636711 - 11/24/18 04:32 PM (5 years, 2 months ago)

If I do clones of something with a meaty cap I prefer that. It tends to cut out nicely.


--------------------
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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Re: Cloning Wild Fruits [Re: 36fuckin5]
    #25636770 - 11/24/18 04:56 PM (5 years, 2 months ago)

:whathesaid:
This, cap material is very nice to cut compared to stipe material


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Cloning Wild Fruits [Re: cronicr]
    #26318367 - 11/13/19 06:39 PM (4 years, 2 months ago)

NIce thanks brother


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Re: Cloning Wild Fruits [Re: Cocoa0113]
    #26318369 - 11/13/19 06:39 PM (4 years, 2 months ago)

:rockon:


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Re: Cloning Wild Fruits (moved) [Re: YaMoonSun]
    #26454843 - 01/27/20 09:35 AM (4 years, 3 days ago)

Quote:

YaMoonSun said:
I've got two jars I wanna do invitro. They're fully colonized but smell a tad bit bacterial. Should I birth them into a bleach water rinse and then clean the jars, and put them back into the jars with a vermiculite casing layer on top?




What's your bleach to water ratio?


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It's the Journey I enjoy!


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Re: Cloning Wild Fruits [Re: cronicr]
    #26712180 - 06/01/20 07:03 PM (3 years, 7 months ago)

Exactly what i needed to read a couple of days ago!

Put grossery oysters to water agar, something that looks like bacteria all around and no mycelium stretching out.

This tech comming up next!

I have a project ressearching antibacterial properties of some plants in agar. Guess this would be the perfect application to try my hypothesis on.

Greetings


--------------------
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