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OfflinecronicrFacebook
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Registered: 08/07/11
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Cloning Wild Fruits * 12
    #22377031 - 10/14/15 04:56 AM (8 years, 7 months ago)

Going to doc a couple ways I like to do this, from typical cloning to wild fruits to dried fruits.
To start this I got my sterile water ready, two small ones have a wee bit of bleach
.
From there I will either take the two small bottles with me in the field or just use them at home but they are my starting point.

When in the field I simply cut the cap off after pulling the mushroom from the ground, I then wipe any debris off the base  and will drop the tissue in the water and shake, this rinses off a lot of contams and the bleach helps as well.

The next step is removing it once home and putting it in another jar of sterile water and shaking a few times, this step can be repeated a couple times and this greatly waters down any contams present and gives your tissue sample a much better chance and without things like Iso and iodine which may make things harder!

So we took some gyms and did steps one and two and proceeded to clone like normal taking the last sterile water jar with the tissue sample to the sab for final removal before cloning to no pour Petri's with premixed mea.

After wiping down the water jars and agar jars in iso and prepping the sab like normal I took the tissue sample out from the water and tore it in half from the top down and put three samples in three agar jars.
Within 3 days I had great growth in them and was ready for the first transfer in just four days.



A few transfers in....

.

ready for grain

for the first run though i'm going to go to supplemented jars(wheat gypsum coffee bran wodchips)
\
Here i did this with an Agaricus mushroom.

Cut the stipe

Add to jar 1 and shake

Transfer to jar 2 and shake and repeat for about 3 jars of water/bleach

Then take it out and place it in your prepped sab.

Tare it from the top down in half

Take your tissue sample from the inner base

Place on agar wait for growth.

Here's a quick over view on how i did the dry fruit clone......no different from what you see above otherthen the first soak sits for a bit

dried fruit


and gold




--------------------

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OfflinePsilosopherr
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Re: Cloning wild fruits [Re: cronicr]
    #22447505 - 10/29/15 12:53 AM (8 years, 6 months ago)

:popcorn: First! :crondance:

Edited by Psilosopherr (09/09/16 08:07 PM)

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OfflinecronicrFacebook
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22507985 - 11/11/15 04:37 AM (8 years, 6 months ago)

This thread was moved from the user's journal.

Reason:
Why must I always have a reason:smbfacepalm:

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Invisibleoontribe
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Registered: 01/14/15
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22508017 - 11/11/15 05:20 AM (8 years, 6 months ago)

thanks for sharing man :smile:

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Invisiblewowimflabbergasted
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Re: Cloning Wild Fruits (moved) [Re: oontribe]
    #22508222 - 11/11/15 07:41 AM (8 years, 6 months ago)

Sweet nice pic tutorial cron!


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Offlinespacechildo
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Re: Cloning Wild Fruits (moved) [Re: wowimflabbergasted]
    #22508774 - 11/11/15 10:43 AM (8 years, 6 months ago)

aaaw yeah, here we go!

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Offlinethebug76
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Re: Cloning Wild Fruits (moved) [Re: spacechildo]
    #22508838 - 11/11/15 11:01 AM (8 years, 6 months ago)

Just making sure I can find this again.


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Bug 

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Invisible36fuckin5
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Re: Cloning Wild Fruits (moved) [Re: thebug76] * 1
    #22509356 - 11/11/15 01:27 PM (8 years, 6 months ago)

But you forgot the part that makes it hard...

Oh wait, that isn't a step. It's really this easy, folks.


--------------------
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A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflineDarkhome
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Re: Cloning Wild Fruits (moved) [Re: 36fuckin5]
    #22509406 - 11/11/15 01:40 PM (8 years, 6 months ago)

:takingnotes:Good info! Thanx!


--------------------
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"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.

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OfflineSivarted
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Re: Cloning Wild Fruits (moved) [Re: Darkhome]
    #22509546 - 11/11/15 02:12 PM (8 years, 6 months ago)

I've seen a couple different posts about cloning wild mushrooms, and I'm having trouble seeing why cloning a wild one would be any different than cloning one from an indoor grow.  Yeah, maybe outside is dirtier and more contaminated (although after accidentally seeing the some of the shit that is more than happy to grow on agar in my bedroom, I might challenge that statement), but if the steps for cloning a promising mushroom from your grow (or really, for anything involving contams, ive had to follow this process to get something out of a horribly contaminated syringe) are:

Step 1: Get a bit of interior tissue onto agar
Step 2: transfer away from contams, repeat until you have a clean culture

Then why do wild mushrooms require special treatment?

I'm not being at all dismissive here, this is an honest question, because I'm probably going to hunt edible species in my area at some point.

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InvisiblefahtsterM
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Re: Cloning Wild Fruits (moved) [Re: Sivarted]
    #22509587 - 11/11/15 02:21 PM (8 years, 6 months ago)

sweeet. :thumbup:

faht

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InvisiblePastywhyteMDiscord
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22509601 - 11/11/15 02:24 PM (8 years, 6 months ago)

Quote:

cronicr said:
<img src='https://www.shroomery.org/forums/images/moved.gif'> This thread was moved from the user's journal.

Reason:
Why must I always have a reason:smbfacepalm:




:lolsy: sounds like some first world problems. Perhaps :rockon: will suffice.

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Offlineinvitro

Registered: 05/03/13
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Re: Cloning Wild Fruits [Re: cronicr]
    #22509611 - 11/11/15 02:28 PM (8 years, 6 months ago)

:thatsinteresting:

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OfflinecronicrFacebook
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Re: Cloning Wild Fruits (moved) [Re: Sivarted]
    #22509754 - 11/11/15 03:08 PM (8 years, 6 months ago)

:lol:
Quote:

Sivarted said:
I've seen a couple different posts about cloning wild mushrooms, and I'm having trouble seeing why cloning a wild one would be any different than cloning one from an indoor grow.  Yeah, maybe outside is dirtier and more contaminated (although after accidentally seeing the some of the shit that is more than happy to grow on agar in my bedroom, I might challenge that statement), but if the steps for cloning a promising mushroom from your grow (or really, for anything involving contams, ive had to follow this process to get something out of a horribly contaminated syringe) are:

Step 1: Get a bit of interior tissue onto agar
Step 2: transfer away from contams, repeat until you have a clean culture

Then why do wild mushrooms require special treatment?

I'm not being at all dismissive here, this is an honest question, because I'm probably going to hunt edible species in my area at some point.



Outdoors is strait up dirty! i can't remember the numbers exactly but trust me it's friggiun insane how many contams can be found inside and outside of a fruit:crazy:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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OfflineMycologist217
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22509758 - 11/11/15 03:10 PM (8 years, 6 months ago)

You da man Cronicr!


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OfflinecronicrFacebook
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Re: Cloning Wild Fruits (moved) [Re: Mycologist217]
    #22509778 - 11/11/15 03:13 PM (8 years, 6 months ago)

found a sweet patch across the street from me of some edibles i'm going to dive in on tonight, i'll get more pics up of the ones i did already there is great looking growth!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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InvisibleAdden
I'm a teapot
Registered: 06/04/03
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
    #22509850 - 11/11/15 03:27 PM (8 years, 6 months ago)

:threadmonitor:

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OfflineMoldy thumb
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Re: Cloning Wild Fruits (moved) [Re: Adden] * 1
    #22509921 - 11/11/15 03:44 PM (8 years, 6 months ago)

:takingnotes:


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InvisibleAdden
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Registered: 06/04/03
Posts: 39,201
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Re: Cloning Wild Fruits (moved) [Re: Moldy thumb]
    #22509933 - 11/11/15 03:45 PM (8 years, 6 months ago)

Haha, yep!

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OfflineYaMoonSun
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Re: Cloning Wild Fruits (moved) [Re: Adden]
    #22526970 - 11/15/15 10:21 AM (8 years, 6 months ago)

So, do I sterilize a container and then poor the sterile water into the said sterile container, and let it cool few a few hours before applying the mushroom to the water? Or do I sterilize the water using a cap of bleach and just toss the mycelium into it?

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