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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Cloning Wild Fruits 12
#22377031 - 10/14/15 04:56 AM (8 years, 7 months ago) |
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Going to doc a couple ways I like to do this, from typical cloning to wild fruits to dried fruits. To start this I got my sterile water ready, two small ones have a wee bit of bleach
. From there I will either take the two small bottles with me in the field or just use them at home but they are my starting point.
When in the field I simply cut the cap off after pulling the mushroom from the ground, I then wipe any debris off the base and will drop the tissue in the water and shake, this rinses off a lot of contams and the bleach helps as well.
 The next step is removing it once home and putting it in another jar of sterile water and shaking a few times, this step can be repeated a couple times and this greatly waters down any contams present and gives your tissue sample a much better chance and without things like Iso and iodine which may make things harder!
 So we took some gyms and did steps one and two and proceeded to clone like normal taking the last sterile water jar with the tissue sample to the sab for final removal before cloning to no pour Petri's with premixed mea.
 After wiping down the water jars and agar jars in iso and prepping the sab like normal I took the tissue sample out from the water and tore it in half from the top down and put three samples in three agar jars. Within 3 days I had great growth in them and was ready for the first transfer in just four days.


A few transfers in....

.
 ready for grain
 for the first run though i'm going to go to supplemented jars(wheat gypsum coffee bran wodchips)
\  Here i did this with an Agaricus mushroom.
  Cut the stipe
 Add to jar 1 and shake
 Transfer to jar 2 and shake and repeat for about 3 jars of water/bleach
 Then take it out and place it in your prepped sab.
 Tare it from the top down in half
 Take your tissue sample from the inner base
 Place on agar wait for growth.
Here's a quick over view on how i did the dry fruit clone......no different from what you see above otherthen the first soak sits for a bit
 dried fruit

 and gold

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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,280
Last seen: 1 month, 30 days
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Re: Cloning wild fruits [Re: cronicr]
#22447505 - 10/29/15 12:53 AM (8 years, 6 months ago) |
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First!
Edited by Psilosopherr (09/09/16 08:07 PM)
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cronicr


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
#22507985 - 11/11/15 04:37 AM (8 years, 6 months ago) |
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This thread was moved from the user's journal.
Reason: Why must I always have a reason
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oontribe

Registered: 01/14/15
Posts: 3,570
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
#22508017 - 11/11/15 05:20 AM (8 years, 6 months ago) |
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thanks for sharing man
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Cloning Wild Fruits (moved) [Re: oontribe]
#22508222 - 11/11/15 07:41 AM (8 years, 6 months ago) |
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Sweet nice pic tutorial cron!
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 8 months
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aaaw yeah, here we go!
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 7 months, 12 days
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Re: Cloning Wild Fruits (moved) [Re: spacechildo]
#22508838 - 11/11/15 11:01 AM (8 years, 6 months ago) |
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Just making sure I can find this again.
-------------------- Bug
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,091
Loc: Diving into Mystical Territori...
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Re: Cloning Wild Fruits (moved) [Re: thebug76] 1
#22509356 - 11/11/15 01:27 PM (8 years, 6 months ago) |
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But you forgot the part that makes it hard...
Oh wait, that isn't a step. It's really this easy, folks.
-------------------- Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
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Re: Cloning Wild Fruits (moved) [Re: 36fuckin5]
#22509406 - 11/11/15 01:40 PM (8 years, 6 months ago) |
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Good info! Thanx!
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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Sivarted
Stranger
Registered: 08/22/15
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Re: Cloning Wild Fruits (moved) [Re: Darkhome]
#22509546 - 11/11/15 02:12 PM (8 years, 6 months ago) |
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I've seen a couple different posts about cloning wild mushrooms, and I'm having trouble seeing why cloning a wild one would be any different than cloning one from an indoor grow. Yeah, maybe outside is dirtier and more contaminated (although after accidentally seeing the some of the shit that is more than happy to grow on agar in my bedroom, I might challenge that statement), but if the steps for cloning a promising mushroom from your grow (or really, for anything involving contams, ive had to follow this process to get something out of a horribly contaminated syringe) are:
Step 1: Get a bit of interior tissue onto agar Step 2: transfer away from contams, repeat until you have a clean culture
Then why do wild mushrooms require special treatment?
I'm not being at all dismissive here, this is an honest question, because I'm probably going to hunt edible species in my area at some point.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,401
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Re: Cloning Wild Fruits (moved) [Re: Sivarted]
#22509587 - 11/11/15 02:21 PM (8 years, 6 months ago) |
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sweeet. 
faht
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,867
Loc: Canada
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
#22509601 - 11/11/15 02:24 PM (8 years, 6 months ago) |
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Quote:
cronicr said: <img src='https://www.shroomery.org/forums/images/moved.gif'> This thread was moved from the user's journal.
Reason: Why must I always have a reason
sounds like some first world problems. Perhaps will suffice.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 5 months, 7 days
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Re: Cloning Wild Fruits [Re: cronicr]
#22509611 - 11/11/15 02:28 PM (8 years, 6 months ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Re: Cloning Wild Fruits (moved) [Re: Sivarted]
#22509754 - 11/11/15 03:08 PM (8 years, 6 months ago) |
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Quote:
Sivarted said: I've seen a couple different posts about cloning wild mushrooms, and I'm having trouble seeing why cloning a wild one would be any different than cloning one from an indoor grow. Yeah, maybe outside is dirtier and more contaminated (although after accidentally seeing the some of the shit that is more than happy to grow on agar in my bedroom, I might challenge that statement), but if the steps for cloning a promising mushroom from your grow (or really, for anything involving contams, ive had to follow this process to get something out of a horribly contaminated syringe) are:
Step 1: Get a bit of interior tissue onto agar Step 2: transfer away from contams, repeat until you have a clean culture
Then why do wild mushrooms require special treatment?
I'm not being at all dismissive here, this is an honest question, because I'm probably going to hunt edible species in my area at some point.
Outdoors is strait up dirty! i can't remember the numbers exactly but trust me it's friggiun insane how many contams can be found inside and outside of a fruit
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 2 days, 16 hours
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
#22509758 - 11/11/15 03:10 PM (8 years, 6 months ago) |
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You da man Cronicr!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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found a sweet patch across the street from me of some edibles i'm going to dive in on tonight, i'll get more pics up of the ones i did already there is great looking growth!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Cloning Wild Fruits (moved) [Re: cronicr]
#22509850 - 11/11/15 03:27 PM (8 years, 6 months ago) |
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Moldy thumb
Necromancer



Registered: 08/05/15
Posts: 199
Last seen: 6 years, 10 months
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Re: Cloning Wild Fruits (moved) [Re: Adden] 1
#22509921 - 11/11/15 03:44 PM (8 years, 6 months ago) |
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--------------------
My Trade List
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Cloning Wild Fruits (moved) [Re: Moldy thumb]
#22509933 - 11/11/15 03:45 PM (8 years, 6 months ago) |
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Haha, yep!
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YaMoonSun
The Double Standard


Registered: 10/23/14
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Loc: NY
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Re: Cloning Wild Fruits (moved) [Re: Adden]
#22526970 - 11/15/15 10:21 AM (8 years, 6 months ago) |
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So, do I sterilize a container and then poor the sterile water into the said sterile container, and let it cool few a few hours before applying the mushroom to the water? Or do I sterilize the water using a cap of bleach and just toss the mycelium into it?
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