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OfflineSmoothcat
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First ever mono tub (b+ with pictures)
    #22375271 - 10/13/15 07:32 PM (8 years, 3 months ago)

So here we have my first grow log with pictures.

On August 18th I inoculated 5 rye berry jars with B+ from a spore vile that I got from a dutch supplier.

On the night of October 10th my jars were fully colonised. The picture is from a couple of days before full colonisation.


The jars were kept in an airing cupboard that was usually between 20 degs C and 25 degs  C (68f - 79f)

The jars seemed to take forever to colonise, nearly two months, and I think this could have been due to a number of factors (please feel free to tell me i'm wrong). 

Fluctuating temperatures, lack of indirect light, use of multispore and I think the main reason for slow colonisation was lack of GE due to my lid design.

So on October 10th I prepared my monotub using mainly info from TranscendingLife's tub design and changing it a little to fit my tub.

http://www.shroomery.org/forums/showflat.php/Number/14507008/fpart/1/vc/1

And used damion5050's elementary coir tek for the rest and am following it to the t

http://www.shroomery.org/10858/How-to-do-Coir

The only thing I did differently was not save a top layer of coir and just mixed it all together.

Here's how the tub looked just after spawning:


And this is inside the tub three days later:




I plan to update this post with pictures and results and any tips or observations off you more experienced growers would be most welcome and hopefully i'll be able to pull this off and we'll have pins :mushroom2:

I've been reading this site for years and never quite took the plunge but now I have my own place I thought it was time to dive in :cool: don't think I would have even made it this far without this site :cool:

Now we wait :crazy2:


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OfflinePsilosoulful

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Re: First ever mono tub (b+ with pictures) [Re: Smoothcat]
    #22375336 - 10/13/15 07:43 PM (8 years, 3 months ago)

:woot:

The surface of the tub is showing nice strong growth so far, should be done in less than a week!

How did you make your lids? Lack of proper GE would definitely contribute to stalled growth.


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OfflineRoostertail

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Re: First ever mono tub (b+ with pictures) [Re: Psilosoulful]
    #22375449 - 10/13/15 08:04 PM (8 years, 3 months ago)

Nice job.

I'm weighing out whether I would like to mix or not. I do have the coir tek bookmarked.

Keep us posted on how it turns out.

Do you have to mist?


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OfflineSmoothcat
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Re: First ever mono tub (b+ with pictures) [Re: Psilosoulful]
    #22375492 - 10/13/15 08:15 PM (8 years, 3 months ago)

Quote:

Psilosoulful said:
:woot:

The surface of the tub is showing nice strong growth so far, should be done in less than a week!

How did you make your lids? Lack of proper GE would definitely contribute to stalled growth.





Less than a week would be amazing!! I'll keep you updated with pictures :sun:

I did four of the lids with four holes for GE and one for innoc but as my first grow I think I got a bit contam paranoid and went to town with the micropore tape. I had even micropored the holes on the inside of the jar before PC-Ing :blush: I didn't even realise I had done it until I spawned them!

I think it was definitely poor GE as I had nearly given up hope as there had been no growth for a week so I cracked the lids and amazingly growth took off again in a big way :thumbup:

I wonder if by having high co2 during colonisation of the jars will give me super growth in the tub?? :crazy2:

My 5th jar was a bit of a cheat as I ordered it from a supplier before I realised I could actually go to the shop and purchase the jars my self. It had a SHIP and a chunk of polyfill in one larger hole. Although that one seemed to go as slow as the others as well.

Oh well, tis all a learning curve I suppose :tongue:


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OfflineSmoothcat
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Re: First ever mono tub (b+ with pictures) [Re: Roostertail]
    #22375546 - 10/13/15 08:23 PM (8 years, 3 months ago)

Quote:

Roostertail said:
Nice job.

I'm weighing out whether I would like to mix or not. I do have the coir tek bookmarked.

Keep us posted on how it turns out.

Do you have to mist?




Definitely go for damion5050 coir tek, from what I read, and hopefully soon from personal experience, people have a lot of success with it.

The great thing about it as well is that it's all self contained, no fanning and misting etc, I think the only time you ever mist it is when your looking for a second flush or if you've prepared your substrate or your tub incorrectly and it drys out. But I don't think that will happen if you follow the teks :smile:


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OfflineRoostertail

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Re: First ever mono tub (b+ with pictures) [Re: Smoothcat]
    #22375601 - 10/13/15 08:32 PM (8 years, 3 months ago)

Quote:

Smoothcat said:
Quote:

Roostertail said:
Nice job.

I'm weighing out whether I would like to mix or not. I do have the coir tek bookmarked.

Keep us posted on how it turns out.

Do you have to mist?




Definitely go for damion5050 coir tek, from what I read, and hopefully soon from personal experience, people have a lot of success with it.

The great thing about it as well is that it's all self contained, no fanning and misting etc, I think the only time you ever mist it is when your looking for a second flush or if you've prepared your substrate or your tub incorrectly and it drys out. But I don't think that will happen if you follow the teks :smile:




Are you talking about the monotub tek your using. I still dont how I want my tub.

I studied an alien tek a while back which used the Alien tek and 50/50 casing

My plan so far is to use poly fil or whatever its called with 2-3 inch drilled holes.

Let me know how yours turns out.

Im on for the ride!!:thanx:


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Offlinegraciousdog
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Re: First ever mono tub (b+ with pictures) [Re: Roostertail]
    #22375653 - 10/13/15 08:43 PM (8 years, 3 months ago)

Hey, I am thinking of starting a monotub for my next grow, but it probably won't be for a while. Regardless, I want to know, when following Damion5050's tek, did you pasteurize the coir/verm mix or did you just simply pour boiling water into it?


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OfflineRoostertail

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Re: First ever mono tub (b+ with pictures) [Re: graciousdog]
    #22375733 - 10/13/15 08:57 PM (8 years, 3 months ago)



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OfflineSmoothcat
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Re: First ever mono tub (b+ with pictures) [Re: Roostertail]
    #22375741 - 10/13/15 08:58 PM (8 years, 3 months ago)






Quote:
Are you talking about the monotub tek your using. I still dont how I want my tub.

I studied an alien tek a while back which used the Alien tek and 50/50 casing

My plan so far is to use poly fil or whatever its called with 2-3 inch drilled holes.

Let me know how yours turns out.

Im on for the ride!!:thanx:




Both teks I used are in the post, the only difference was where I drilled the holes on the tub as you want them to be substrate level, so you will need to work that out but it's easy enough and can be measured using water.

During colonisation as well you want your holes covered with duck tape or similar (I used electrical tape) and when it's colonised that's when I'll take the tape off and insert the polyfill.

Basically I'm just following the teks and its my first time so I'll keep ya posted :smile: hopefully we'll see pins :mushroom2:


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OfflineSmoothcat
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Re: First ever mono tub (b+ with pictures) [Re: graciousdog]
    #22375772 - 10/13/15 09:04 PM (8 years, 3 months ago)

Quote:

graciousdog said:
Hey, I am thinking of starting a monotub for my next grow, but it probably won't be for a while. Regardless, I want to know, when following Damion5050's tek, did you pasteurize the coir/verm mix or did you just simply pour boiling water into it?





Just followed the tek :cool: I think it was 4 quarts of water, boiled on the stove then added to the coir brick and 2 quarts verm and left for half an hour before mixing thoroughly.

I sealed the lid on my bucket as soon as I added the water, the heat or gases from the hydrating coir blew the lid off my bucket with an almighty bang :eek: so I may not seal it as tight next time.

But so far it all seems to be going well :grin:


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OfflineRoostertail

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Re: First ever mono tub (b+ with pictures) [Re: Smoothcat]
    #22375789 - 10/13/15 09:08 PM (8 years, 3 months ago)

Cant go wrong with scolding hot boiling water. :thumbinator:


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OfflineSmoothcat
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Re: First ever mono tub (b+ with pictures) [Re: Roostertail]
    #22375794 - 10/13/15 09:09 PM (8 years, 3 months ago)

Quote:

Roostertail said:
This what I plan on using with rye.

http://www.shroomery.org/forums/showflat.php/Number/12968957/vc/1#12968957




Looks good, quicker than the way I did it. I did the 24hour soak and RR's method.

Let me know how you get on with it, always good to save a bit of time :cool:


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OfflinePsilosoulful

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Re: First ever mono tub (b+ with pictures) [Re: Smoothcat]
    #22376412 - 10/13/15 11:13 PM (8 years, 3 months ago)

Quote:

Smoothcat said:
Less than a week would be amazing!! I'll keep you updated with pictures :sun:



:super:


Quote:

Smoothcat said:
I did four of the lids with four holes for GE and one for innoc but as my first grow I think I got a bit contam paranoid and went to town with the micropore tape. I had even micropored the holes on the inside of the jar before PC-Ing :blush: I didn't even realise I had done it until I spawned them!



Micropore tape is a pretty weak GE filter, as it is very porous, and contams can still pass through. I highly recommend looking into SFD's. They've worked great for me and I've seen them used frequently by most TC's and other cultivators.

Micropore on the INSIDE of a grain jar is a bad idea. I'm surprised your jar didn't contaminate right away, perhaps some bacteria got in, you'll find out later.

Shaking the grains will wet the tape and it will be a breeding ground for contams to grow, as it is not waterproof.


Quote:

Smoothcat said:
I think it was definitely poor GE as I had nearly given up hope as there had been no growth for a week so I cracked the lids and amazingly growth took off again in a big way :thumbup:



I hope you cracked the jar lid open inside of your SAB, because if not, the results will not be pretty, as contams will fly right into the jar.

Like I said before, you'll find out later in the fruiting stage.

From the pics, the mycelium looks to be healthy, but you never know what's hiding beneath the surface.


Quote:

Smoothcat said:
I wonder if by having high co2 during colonisation of the jars will give me super growth in the tub?? :crazy2:



Grain jars are supposed to have high CO2 levels during colonization, with some GE.

Genetics plays the biggest role in determining the fruiting potential of the tub. When you start from MS, you never know what you're gonna get. The yield could be great, terrible, and everything in between. It's just a roll of the dice.

Adequate FAE and lighting are other factors that play a significant role, and must be supplied properly, so the full potential of the mycelium can be expressed.



Quote:

Smoothcat said:
My 5th jar was a bit of a cheat as I ordered it from a supplier before I realised I could actually go to the shop and purchase the jars my self. It had a SHIP and a chunk of polyfill in one larger hole. Although that one seemed to go as slow as the others as well.



I remember using SHIP's when I first started with grain jars.

It wasn't until I made a functional SAB, that I realized that it would be just as simple to crack the lid in there, and inoculate directly.

I mean, you're gonna have to do some G2G transfers and agar work eventually, might as well get comfortable practicing your sterile techniques inside the SAB now.



Quote:

Smoothcat said:
Oh well, tis all a learning curve I suppose :tongue:



It definitely is. I'm still learning new things everyday, and I been on here for like a year!

I love this hobby, wouldn't trade it for anything else. I know you feel the same too (based on your sig lol).  :shroomeryhead:


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OfflineSmoothcat
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Re: First ever mono tub (b+ with pictures) [Re: Psilosoulful]
    #22377187 - 10/14/15 06:54 AM (8 years, 3 months ago)

Quote:

Micropore tape is a pretty weak GE filter, as it is very porous, and contams can still pass through. I highly recommend looking into SFD's. They've worked great for me and I've seen them used frequently by most TC's and other cultivators.




Yes I realise this now, I do now have SFD's but I'd already made my jars up and people had said micropore was ok, definitely using the discs next time :rolleyes:

Quote:

I remember using SHIP's when I first started with grain jars.

It wasn't until I made a functional SAB, that I realized that it would be just as simple to crack the lid in there, and inoculate directly.

I mean, you're gonna have to do some G2G transfers and agar work eventually, might as well get comfortable practicing your sterile techniques inside the SAB now.

I hope you cracked the jar lid open inside of your SAB, because if not, the results will not be pretty, as contams will fly right into the jar.




I don't have an SAB :sad: I just ousted everywhere and was as sterile as possible, doors windows closed Lysol etc. I'm probably 101 on how to cowboy the whole process :blush:

I want to do more in this like g2g and agar etc so it looks like I'll need to be building an SAB for next time :tongue:

Quote:

Like I said before, you'll find out later in the fruiting stage.

From the pics, the mycelium looks to be healthy, but you never know what's hiding beneath the surface.




Exactly, everything has looked right, no visible signs of contams and the tub looks good but we will have to wait to fruiting (if any) to find out :sad:

Fingers crossed, updates to follow :ooo:


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OfflinePsilosoulful

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Re: First ever mono tub (b+ with pictures) [Re: Smoothcat]
    #22380299 - 10/14/15 07:15 PM (8 years, 3 months ago)

You will need a SAB for your future grows, if you want consistent success and not just luck.

Contam rates are still very high during open air inoculations, even if proper precautions are taken.

SAB's are soo simple to make. Just get a 66qt tote or larger, and use a hole saw to cut two large holes for your hands to fit through (I just heat up the bottom of an empty tomato can and melt it through, it's a lot easier :wink: ). Make sure to off-set the holes, so you can maneuver around the SAB easier.

Here's my SAB, it also has added tyvek sleeves and a cover, it helps prevent air currents from going inside:




When you're ready to use it, place all your materials inside, then spray it down with soapy water to trap contams against the sides of the box and let it sit for a few minutes for the air to settle. Remember, still air is key to success, so work slowly in the box, no fast movements. :super:


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OfflineSmoothcat
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Re: First ever mono tub (b+ with pictures) [Re: Psilosoulful]
    #22380497 - 10/14/15 07:52 PM (8 years, 3 months ago)

This is why I love this site and the people who post on here :smile:

I just happen to have a spare tub and love your easy way of cutting the holes.

You've inspired me! This will be my next piece of kit for my next experiments :tongue:

:thanx:


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InvisibleLocN9ne
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Re: First ever mono tub (b+ with pictures) [Re: Smoothcat]
    #22380647 - 10/14/15 08:17 PM (8 years, 3 months ago)

Fuck yeah:vibin:


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OfflinePsilosoulful

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Re: First ever mono tub (b+ with pictures) [Re: LocN9ne]
    #22380689 - 10/14/15 08:25 PM (8 years, 3 months ago)

:awethumb:


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Re: First ever mono tub (b+ with pictures) [Re: Psilosoulful]
    #22387137 - 10/16/15 06:17 AM (8 years, 3 months ago)

So here's an update on the tubs progress so far :cool:

So it was spawned on the night of the 10th and today is the 16th so I suppose you could say it's 5.5 days in.

Pics:





Now I know it's a bit difficult to see through the tub what with all the condensation but I don't want to crack the lid just yet, maybe in a couple more days I will.

But basically it's almost completely colonised with just a few patches left to go which hopefully will be done in the next day or so :grin:

Soo excited :crazy2:


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OfflinePsilosoulful

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Re: First ever mono tub (b+ with pictures) [Re: Smoothcat]
    #22387201 - 10/16/15 06:56 AM (8 years, 3 months ago)

:thisisgonnabegood:


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