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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Should I Agar? [Re: wowimflabbergasted]
    #22374008 - 10/13/15 03:37 PM (8 years, 3 months ago)

It should be noted that bodhis short PC times for agar are based off of the use of lab grade MEA. For people making their own with food grade ingredients and possible solids or high endospore ingredients such as grain water, a 15 min PC cycle may screw you. Also take note of your elevations. Higher above sea level = lower boiling point. I'm only about 2100 ft above sea level but water boils here at around 200 f even.

A month ago I was making PDA from potato flakes and shorted my cycle to 30 min. Bacteria overran every plate including ones I hadn't used yet.


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Invisiblewowimflabbergasted
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Registered: 07/16/12
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Re: Should I Agar? [Re: Pastywhyte]
    #22374083 - 10/13/15 03:54 PM (8 years, 3 months ago)

I personally do 40 minutes.


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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