Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineThatShroomer7
Stranger
 User Gallery


Registered: 10/01/15
Posts: 61
Last seen: 2 days, 6 hours
Should I Agar?
    #22371948 - 10/13/15 06:54 AM (8 years, 3 months ago)

I got about 3 syringes coming the mail of various spores , and 4jars. I'm ready to knock these babies up! But people have been advising me to use agar to isolate the best myc. But in my mind that seems very time consuming and will extend the time I will have to wait for some fruits !

I'm so use to jumping into it and injecting with syringes , I've never really had any problems. But I was curious about this agar thing .
Should I honestly take all this time to purchase all the supplies , and everything . or just go ahead and inject in my SAB today once the syringes arrive ? Let me know what you guys think .


--------------------


Extras: Filter Print Post Top
OfflineAchillita
Back to the basics
Male


Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 10 days
Re: Should I Agar? [Re: ThatShroomer7]
    #22371957 - 10/13/15 06:58 AM (8 years, 3 months ago)

Agar is great. I've dropped my contamination rate by quite a bit. And have been able to save clones that are really good.

Definitely recommended.


--------------------


Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Should I Agar? [Re: Achillita]
    #22371969 - 10/13/15 07:06 AM (8 years, 3 months ago)

Agar needn't take a lot of supplies or time if you follow the right tek:smile:
http://www.shroomery.org/forums/showflat.php/Number/19208976


Extras: Filter Print Post Top
OfflineThatShroomer7
Stranger
 User Gallery


Registered: 10/01/15
Posts: 61
Last seen: 2 days, 6 hours
Re: Should I Agar? [Re: Shroomspective]
    #22371972 - 10/13/15 07:10 AM (8 years, 3 months ago)

Love the easy tek. Only thing that sucks is no PC! I need to invest !


--------------------


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: Should I Agar? [Re: ThatShroomer7]
    #22372038 - 10/13/15 07:45 AM (8 years, 3 months ago)

You don't NEED a PC for agar.


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
InvisibleDaveyJones6911
Nonconformist
 User Gallery

Registered: 09/08/15
Posts: 690
Loc: EU Flag
Re: Should I Agar? [Re: ThatShroomer7]
    #22372045 - 10/13/15 07:49 AM (8 years, 3 months ago)

just use the syringes but make sure to leave like 1cc or so in them
to use with agar. you don't need much, and that will give you something to
do while you wait for the first grow colonise etc.

Quote:

ThatShroomer7 said:
Love the easy tek. Only thing that sucks is no PC! I need to invest !




i'm just getting into agar myself and i don't have a PC.
so far it doesn't seem to be a problem steaming the old fashioned way.


--------------------
Creativity is a dangerous thing in the eyes of those who don't have any.


Extras: Filter Print Post Top
OfflineThatShroomer7
Stranger
 User Gallery


Registered: 10/01/15
Posts: 61
Last seen: 2 days, 6 hours
Re: Should I Agar? [Re: DaveyJones6911]
    #22372193 - 10/13/15 08:43 AM (8 years, 3 months ago)

I'll inject these in my SAB . and like you said pratice while I'm waiting for my jars to colinoize . I'll order another 4 next week , just in case I get contam on these first four!, I will try to steam too hope it works alright ! I would love to isolate some good spores


--------------------


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: Should I Agar? [Re: ThatShroomer7]
    #22372254 - 10/13/15 09:03 AM (8 years, 3 months ago)

How did you get that growing mushroom under your name???


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
OfflineThatShroomer7
Stranger
 User Gallery


Registered: 10/01/15
Posts: 61
Last seen: 2 days, 6 hours
Re: Should I Agar? [Re: wowimflabbergasted]
    #22372475 - 10/13/15 10:00 AM (8 years, 3 months ago)

Its an emoji , I found it on the site and added it to my avi


--------------------


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: Should I Agar? [Re: ThatShroomer7]
    #22372549 - 10/13/15 10:16 AM (8 years, 3 months ago)

Oh duh lol :facepalm:


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
InvisibleKalistis
 User Gallery

Registered: 09/06/15
Posts: 2,265
Re: Should I Agar? [Re: wowimflabbergasted]
    #22372616 - 10/13/15 10:36 AM (8 years, 3 months ago)

Hi Shroomer... I wouldn't rush out to get more syringes just yet. My first 4 syringes have kept me very busy over the past 2 months and so far so good. The syringes go a long way on agar, plus whatever you end up fruiting from MS you can then print and continue the cycle.

So far I have 2 tubs about to fruit, 2 tubs colonizing, 12 plates that have made their 3rd transfer and are now ready to transferred to grain or turned into LI. And I still have 1 untouched syringe and 1 mostly untouched syringe (only a ml) taken for agar.

Did you get all different strains?


--------------------


Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: Should I Agar? [Re: wowimflabbergasted]
    #22372736 - 10/13/15 11:02 AM (8 years, 3 months ago)

Quote:

wowimflabbergasted said:
You don't NEED a PC for agar.




get of your high horse


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: Should I Agar? [Re: blackdust]
    #22372760 - 10/13/15 11:07 AM (8 years, 3 months ago)

Quote:

blackdust said:
Quote:

wowimflabbergasted said:
You don't NEED a PC for agar.




get of your high horse



:confused:


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: Should I Agar? [Re: wowimflabbergasted]
    #22372768 - 10/13/15 11:10 AM (8 years, 3 months ago)

just joking :lol:
but you really dont think that no pours at 2 hours would be good?
It's just a thin layer of media
bod is reported to only PC for 15 min

---i havn't tried any of the above
so im just talking out my ass


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: Should I Agar? [Re: blackdust]
    #22372787 - 10/13/15 11:15 AM (8 years, 3 months ago)

I think bod posted something like this awhile back I copied it to my journal.

PC/Steam Times
BRF jars - PC 60-90 minutes, Steam 90-120 minutes,
Agar - PC 10-15 minutes, Steam 30-45 minutes


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: Should I Agar? [Re: wowimflabbergasted]
    #22372805 - 10/13/15 11:18 AM (8 years, 3 months ago)

i want to try some steam experiments
so I can write a tek for the poor kids
---Kinda to lazy and have no space though hahaha I'm a dreamer


---
Wow, One of my plants look really sad. Growing really slow (like, half the height of the others) and the leaves seem to be curling up... Gonna try and get some pics tonight to see what everyone thinks


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
Trusted Cultivator
Re: Should I Agar? [Re: ThatShroomer7]
    #22372857 - 10/13/15 11:29 AM (8 years, 3 months ago)

The thing that isn't always apparent to new growers (wasnt to me) is t"bvv'vvb"v"    hat spore syringes are rarely clean .  Might even be enough to be put to grain.
If you have only worked with BRF then its no wonder, BRF is pretty forgiving in that aspect, it is actually pretty rare to contaminate PF cakes with most syringes from good vendors.
You might even get away with putting spores straight to grain for a bit too but you will eventually find that is only luck.

Most of my plates inoculated from spore syringe show some bacteria or even mold.
What makes agar such an awesome tool is how you are able to isolate clean growth away from contams onto new plates, you can be relatively sure you are working with a clean culture.

Being able to produce clean grain spawn is everything in this hobby!

Cloning, strain isolation, short and long term storage of live cultures are other awesome reasons to work with agar!


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineiSmkGrnBud
Psychonaut
 User Gallery


Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 1 hour
Re: Should I Agar? [Re: mushpunx]
    #22372882 - 10/13/15 11:32 AM (8 years, 3 months ago)

Agar and you'll never look back. :thumbup:


--------------------
:bigweed:  iSmkGrnBud's Teks  :bigweed:
:mushroomgrow: iSmkGrnBud's Wild Mushroom Finds :mushroomgrow:
:amanita2: The Noob Forum's List of Teks :amanita2:

“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


Extras: Filter Print Post Top
OfflineThatShroomer7
Stranger
 User Gallery


Registered: 10/01/15
Posts: 61
Last seen: 2 days, 6 hours
Re: Should I Agar? [Re: iSmkGrnBud]
    #22373072 - 10/13/15 12:10 PM (8 years, 3 months ago)

It will take me awhile to grasp all the aspects of agar ! I like to know almost everything about something before jumping right in ! But thanks to everyone who commented . I've always to do things right with no contam. This is the first time I've even heard about agar. But Once the hobby gets ahold of you its got you!:grin:

For this attempt I will just knock up the jars w the syringes just like normal and go from there . next month I will get some agar ! For my second tub
This is also my first try at a mono. I normally do just cakes in a SGFC
So wish me luck :horrified:


--------------------


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: Should I Agar? [Re: ThatShroomer7]
    #22373757 - 10/13/15 02:37 PM (8 years, 3 months ago)

:goodluck:


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* INJECTION PORT agar 2,035 4 02/26/05 07:51 PM
by s360
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,156 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,135 8 06/19/20 09:41 PM
by gorilliam
* Will spores germinate in agar? MycoJunkie 14,999 12 11/26/20 11:15 PM
by KKVguitarist
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,285 38 05/11/01 11:08 AM
by MAIA
* Agar inoculation? tatter1090 1,892 7 01/23/05 03:34 PM
by MushroomFriend
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 14,981 25 06/02/16 05:47 PM
by OldSawbonesHisself

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,100 topic views. 25 members, 244 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.012 seconds on 16 queries.