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OfflineAmerican
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Registered: 09/25/15
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Avoiding senescence when Isolating?
    #22369546 - 10/12/15 05:43 PM (8 years, 3 months ago)

I have read a LOT on this forum over the years, and have seen so many conflicting accounts of how long a strain is good for.

If someone were to do the following , would it work?

Avoid Senescence:

(Day 1) Inoculate  10 WBS with Multi Spore Syringe.


(Day 30) "Master" jars now at 100% full colonization.  Take 1 " Master" jar and G2G transfer to 10 more.

(Day 60) Take these 10 jars and G2G transfer to 100 more.



(Day 90) Spawn 100 jars to Damions 50/50 coir verm.


(Day 120) Take a clone from best fruit of the best tub, and make an isolate. Use this isolate/clone/whatever the proper name is as the NEW master. 


1. Is this isolate/clone ALREADY too old?

2. Is senescence going to be a problem?

3. If I wanted to noc up 100+ jars AT THE SAME TIME with an isolated/cloned Strain, how SHOULD I do that?


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InvisibleLocN9ne
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Re: Avoiding senescence when Isolating? [Re: American] * 1
    #22369578 - 10/12/15 05:52 PM (8 years, 3 months ago)

Day 1...inoculate agar dishes with MS.

Day 5-7... (hopefully) have good growth to take a transfer from.

Day 7-12... make another transfer.

Day 16-18... inoculate 10 jars wbs with agar wedges.

Day 30... g2g those CLEAN master jars to as many jars as you want.

Day 35-40... Spawn those jars to tubs.

Day 45-50...fruits said tubs.

Blah blah blah...

Then pick a worthy clone, and start the process over with that clone so you have a general idea what your working with...then make a slant with your most prolific specimen.

I think that sounds about right.


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OfflineiSmkGrnBud
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Re: Avoiding senescence when Isolating? [Re: LocN9ne]
    #22369595 - 10/12/15 05:55 PM (8 years, 3 months ago)

:whathesaid:


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Invisiblewowimflabbergasted
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Re: Avoiding senescence when Isolating? [Re: American]
    #22369596 - 10/12/15 05:55 PM (8 years, 3 months ago)

No it's not already too old and senescence isn't going to be a problem. G2G is your best bet.


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OfflineAmerican
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Re: Avoiding senescence when Isolating? [Re: wowimflabbergasted]
    #22381827 - 10/15/15 02:49 AM (8 years, 3 months ago)

Awesome responses, and just the advice I wanted to hear!

Thanks for the help, fellas!


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InvisibleMad Season
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Re: Avoiding senescence when Isolating? [Re: American]
    #22381838 - 10/15/15 03:02 AM (8 years, 3 months ago)

Haha I think what we need to realise here is that it isn't about time at all. Its about rate of expansion. As it expands, it also ages. Thus cultures stored in the fridge are considered dormant, and don't "age" as much. Keep in mind it can be expanded a shit ton. Look at edible farms. That's why senescence is rarely a concern in our hobby.

This is a really simplified way of saying it. Its a process called cellular expansion/multiplication. Much different from what were used to, which is cellular division.


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