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OfflineAliz
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Registered: 11/05/14
Posts: 21
Last seen: 3 years, 9 months
Sterilzing Syringes with spores inside
    #22369290 - 10/12/15 04:55 PM (8 years, 3 months ago)

I have one year old syringes half used, checking them today I found some air inside !!
is there any way I can sterilize my Syringes before using them without aborting the spores inside?


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Offlinegannondworf
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Registered: 07/21/14
Posts: 93
Last seen: 9 months, 22 days
Re: Sterilzing Syringes with spores inside [Re: Aliz]
    #22369337 - 10/12/15 05:02 PM (8 years, 3 months ago)

I used a half-empty year-old spore syringe 5 days ago on six jars and all of them have germinated. I honestly have no idea about sterilizing a syringe, as I've never done that, but I would suggest using it as you would normally and maybe prepare to lose some jars, but I didn't lose any so there's that.


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OfflineDrCrumbs
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Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
Re: Sterilzing Syringes with spores inside [Re: Aliz]
    #22369344 - 10/12/15 05:03 PM (8 years, 3 months ago)

Sounds like they may have froze?

So they sucked up air. What I would do is tilt upward and get the air out.

Does it look like anything weird is floating around in there that shouldnt be?

You could then either clean up on agar if its super special, or just do a test run on some spawn.


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Offlinegannondworf
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Registered: 07/21/14
Posts: 93
Last seen: 9 months, 22 days
Re: Sterilzing Syringes with spores inside [Re: DrCrumbs]
    #22369367 - 10/12/15 05:08 PM (8 years, 3 months ago)

I've used five different syringes, and all of them came shipped with a tiny bubble of air in the syringe. It's possible that your syringe is not contaminated with what you believe to be bad air.


Edited by gannondworf (10/12/15 05:09 PM)


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OfflineDrCrumbs
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Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
Re: Sterilzing Syringes with spores inside [Re: gannondworf]
    #22369437 - 10/12/15 05:22 PM (8 years, 3 months ago)

Thats a good point.

Right now I'm testing a few syringes that are about 3 years old and have frozen 3 winters in a row. They had small air bubbles in them from the vendor. Well by small I kind of mean more than bubbles like .2CC's.


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