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Aliz
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Registered: 11/05/14
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Rye Grain spawn, fruiting phase concerns !!
#22369175 - 10/12/15 04:38 PM (8 years, 3 months ago) |
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hello my friends, I am preparing for a cultivation, i will use grain spawn technique in which I will inoculate grain jars using spore syringe then transfer the inoculated grains from the jars into a plastic box and will case a layer on top.
I have some questions, 1) what grain options other than Rye and Millet can I use? 2) can I use dry vermiculite for casing? and what other casings can i use? 3) It is not suitable for me to use a fruiting chamber and I am thinking of using a filter bag for fruiting instead (I've seen this used in mycelium ready to fruit boxes sold online), however they put a layer of perlite at the bottom of the plastic box before adding the inoculated grains (substrate), also the directions for these ready to use boxes are to first fill the box with water, close the lid, let it to soak for 12 hours, open the lid, let it drain completely, put the box into the filter bag and let it fruit completely without interfering with it, which is not the case with what I've read here on Shroomery. so how can I prepare my substrate and plastic box for this technique? 4) can I use a normal plastic bag with polyfill instead of the filter bag? as it is not available for me
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iSmkGrnBud
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Re: Rye Grain spawn, fruiting phase concerns !! [Re: Aliz]
#22369368 - 10/12/15 05:09 PM (8 years, 3 months ago) |
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Oats are great and cheap! 
Why is a fruiting chamber not suitable? You have to have somewhere for your mycelium to fruit. You can use a bag, not a good idea. Whatever you do, do not let your mycelium come in contact with perlite, stick to what you read on here, grow kits are fail. I would say you really need to think about making a SGFC, if you're working with trays. Or you can just do a bulk mono but that'll take up just as much space as a SGFC and trays.
If you're growing some of the regular cubes, I wouldn't case.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Aliz
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Registered: 11/05/14
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Re: Rye Grain spawn, fruiting phase concerns !! [Re: iSmkGrnBud]
#22371127 - 10/12/15 10:22 PM (8 years, 3 months ago) |
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my issue is with maintenance not space, so which needs less care SGFC or mono tub? I am only experienced with PF TEK, does the fruiting phase here needs regular fanning and misting like the PF TEK?
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Machiavelliavore
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Registered: 12/08/14
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Re: Rye Grain spawn, fruiting phase concerns !! [Re: Aliz]
#22371600 - 10/13/15 01:19 AM (8 years, 3 months ago) |
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Let me preface by saying that spawning to coir in a monotub is incredibly easy, low maintainence, and will get the best yield out of the spawn by far. If your spawn is good, there's next to no chance your coir will contam of its own accord.
1. Milo, Oats, Wheat Berries, Brown Rice, Grass Seed, Popcorn: Those are the norms. I'm sure there's plenty of others.
2. Well, that verm needs to get wet to be an effective casing one way or another. You can hydrate it to field capacity and put it ontop, or put the dry stuff ontop then mist it to field capacity. Personally I'd prefer the former to have better control and water distribution.
3. Perlite on the bottom won't do much without air moving through it. Most people here seem to think the growkit setups are terrible. One thing you could try is turning your spawn bag into a monotub airflow design. Maybe cut some threaded sections off plastic bottles, insert them into two bot and two top holes on the bag, and stuff with poly as you would a mono. You could also probably just stab or burn with a soldering iron a bunch of holes in the bag above the rye to make a ~monotub shotgun hybrid~ aka MSG design. Those tend to require misting. Casing with verm. Again, spawning to coir will yield much better than straight grain, as it lacks the water capacity to flush out its nutrition. 4. Not sure what context your asking about. Normal bags cannot go through the pressure cooker.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Aliz
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Registered: 11/05/14
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Last seen: 3 years, 9 months
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thank you for your replies  I think I'll go with popcorn as it is the most available. this is my plan, so please if you guys think something is wrong say it,
inoculate popcorn jars using a syringe, after 60%- 100% colonization I'll mix the jar contents with uncolonized pop corn in trays or monotub then will incubate the trays or monotub in dark spot until the surface shows mycelium growth, then I'll start the fruiting conditions either in a fruiting chamber in case of trays or straight in the monotub
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Machiavelliavore
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Re: Rye Grain spawn, fruiting phase concerns !! [Re: Aliz]
#22373229 - 10/13/15 12:53 PM (8 years, 3 months ago) |
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Nope, won't work.
The need for sterility in mush cult is directly related to the amount of uncolonized highly nutritious material you're working with. Basically we're talking grain. All work with grain must be sterile until it is fully colonized (effectively protected/occupied.) Bulk substrates, such as verm, coir, shit, and straw, are not loaded full of starch and thus are not nearly as susceptible to mold and bacterial bloom before the mushroom mycellium colonize it. Think of it like middle eastern countries. If it has oil, all those moldy white people gonna jump on that shit. If it's just a desert with a bunch of Bedouins, the moldy west won't mess with it cause there's nothing to be gained, no resource to exploit.
I'm not really sure what materials you have here. But basically, as a n00b, you want to pressure cook all your grain and inoculate it through injection ports (poly or silicon) with spore solution. Wild Bird Seed (millet + milo) is cheap and highly available, so I would suggest you start with that since it's a better cheaper grain than popcorn. When you have fully colonized whatever amount it is you intend to colonize, spawn it (ie mix colonized grains in with) to coco coir prepared with buckettek.
I would prefer to start with jars. Not sure of the efficacy of spore solution on large mycobags of grain. I think LI/LC/Slury/G2G is generally prefered for them, and those are not tecniques would care to try as a n00b.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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