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OfflineYaMoonSun
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Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Been cultivating bacteria for weeks
    #22368720 - 10/12/15 03:18 PM (8 years, 3 months ago)

You were right, fuck LC, unless you're working with agar, in a SAB; I admit defeat. Guess it's back to the spore syringes for the time being. :shrug:


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22369009 - 10/12/15 04:11 PM (8 years, 3 months ago)

And then I melt my syringe because the steam ran dry :facepalm3:


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InvisibleInocuole
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22369050 - 10/12/15 04:20 PM (8 years, 3 months ago)

How did you go from spore syringe to LC, back to spore syringe?  Is agar warming the bench or what?


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineYaMoonSun
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Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: Inocuole]
    #22369096 - 10/12/15 04:26 PM (8 years, 3 months ago)

Technically I went from spore syringe, to bacterial contamination, and then back to spore syringes. I think I had success once, with a mutant, but that was before I moved, and there's no place with still air here. The AC is constantly on. There's also very disgusting people here who let fully colonized trichoderma sporulate in cups in the sink. They don't grasp the concept of soaking dishes. They're not supposed to be in there for days..

:justkillmenow:


I've had agar offered to me, but I need to go make a SAB beforehand, and get petris, ect - It's really not in my budget.


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InvisibleInocuole
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22369151 - 10/12/15 04:34 PM (8 years, 3 months ago)

If you don't have any still air in your whole place then, and I hate to recommend it, but maybe you should look into a shmuvbox.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: Inocuole]
    #22369180 - 10/12/15 04:39 PM (8 years, 3 months ago)

Know how to get plastic out of a cooking pot? I can sterilize a new syringe today and get a spore syringe ready to go, but I think I ruined my pot. Is there a solvent that'll take it off? Preferably one that's fumes won't kill me.


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OfflineAchillita
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Registered: 05/26/14
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22369212 - 10/12/15 04:44 PM (8 years, 3 months ago)

You can make a SAB with like 4 bucks..


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OfflineDrCrumbs
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Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
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Re: Been cultivating bacteria for weeks [Re: Inocuole]
    #22369216 - 10/12/15 04:44 PM (8 years, 3 months ago)

I was wondering that also.

Were your print to syringe ok?

Cause if you went vendor syringe, then print, then syringe to LC.

print to to syringe may also be an issue.

Oh did you go vendor syringe to spawn, and vendor syringe to LC?

Seems like the LC should have worked just fine?


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: DrCrumbs]
    #22369237 - 10/12/15 04:49 PM (8 years, 3 months ago)

All my spore syringes (home made) turned out great, with the exception of the PE print that I should have transferred to agar first. I don't use vendors.
Quote:

Achillita said:
You can make a SAB with like 4 bucks..



I know, I'm THAT broke. :shrug:


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OfflineDrCrumbs
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Re: Been cultivating bacteria for weeks [Re: DrCrumbs]
    #22369239 - 10/12/15 04:49 PM (8 years, 3 months ago)

I had a friend watch my PC once and ran it dry.
I returned the PC. It was cheap presto and it killed the seal.

It may flake off or chip off, once cool.

Man I know about that dish thing. What I tell em is if the water isnt warm your not soaking, and no food in the sink.


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InvisibleInocuole
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22369270 - 10/12/15 04:53 PM (8 years, 3 months ago)

If a SAB breaks your budget.... I don't even know what to say.  First maybe do something about the moving air.  If not, I don't even know what to recommend other than maybe agar plates with SHIPs or something.

Seriously though, if you can't buy an SAB... then getting silicone and more syringes is out of the question.

I don't know what to say man.  You seem to have internet and time to fuck around. :shrug:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflineDrCrumbs
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Registered: 10/25/11
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Re: Been cultivating bacteria for weeks [Re: DrCrumbs]
    #22369325 - 10/12/15 04:59 PM (8 years, 3 months ago)

Right thats tough man.

Bring in, investors as fellow hobbyists?


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OfflineYaMoonSun
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Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: DrCrumbs]
    #22369458 - 10/12/15 05:25 PM (8 years, 3 months ago)

I wouldn't call them "hobbyists", but I have had someone talk about investing into my grows. I've been letting them take quite a bit of my harvests as of yet. Hoping they'll have some sort of Epiphany :shrug:


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OfflineDrCrumbs
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22369468 - 10/12/15 05:28 PM (8 years, 3 months ago)

Ya can always have em help (maybe with minor stuff even) also, that way if stuff goes wrong its not all on you.


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: DrCrumbs]
    #22369499 - 10/12/15 05:35 PM (8 years, 3 months ago)

I would have to let them observe me for a while and watch Let's Grow Mushrooms before I let them touch anything. It's hard to teach people who have ADHD. They can't pay attention and want to get straight to the point, and therefore wind up cutting corners and having to learn from experiences.


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Invisiblenatedawgnow
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22369516 - 10/12/15 05:38 PM (8 years, 3 months ago)

That is not necessarily the best idea.

If worst comes to worst, there is this thing you can do
where you provide services for a company in exchange for monetary pay.
I find these to be the best way to gain some disposable income.

If this doesn't suite your fancy, then go to a garage sale and offer
the seller 1 dollar for one of the plastic bins they are peddling their
shit out of. Just the other day I bought a basically brand new FoodSaver
for 5 dollars, roll of plastic included. I was shocked at the price
cause they are damn near 70 dollars brand new. Moral is... garage sales, man.


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OfflineDrCrumbs
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Registered: 10/25/11
Posts: 1,619
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Re: Been cultivating bacteria for weeks [Re: natedawgnow]
    #22369555 - 10/12/15 05:46 PM (8 years, 3 months ago)

maybe their job is supply runs.


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: natedawgnow]
    #22369625 - 10/12/15 06:00 PM (8 years, 3 months ago)

Was thinking about posting an ad on craigslist to fix computers, or even in a Facebook group :shrug:


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OfflineDrCrumbs
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Posts: 1,619
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22374653 - 10/13/15 05:43 PM (8 years, 3 months ago)

Bottles and cans if your in college. Dumpster diving.


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InvisibleBig Bear
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Registered: 06/11/14
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Re: Been cultivating bacteria for weeks [Re: YaMoonSun]
    #22374774 - 10/13/15 06:02 PM (8 years, 3 months ago)

Find a cardboard box in a dumpster and make two holes in it.  Cut a window hole too and put Saran Wrap over it.  If you can't find Saran Wrap go dumpster diving and find clear plastic that you can tape over it. 


Free SAB!  All you gotta do is pick up some trash.  Shoot me a pm and I'll send you some mini rounds and a handful of polyfil.

No more excuses. Agar is the way, the truth, and the light.  You should see all the stuff floating around in your air.


--------------------
Need help growing? Ask AMU for hassle free answers.

Every year is getting shorter, never seem to find the time...


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
Re: Been cultivating bacteria for weeks [Re: Big Bear]
    #22374905 - 10/13/15 06:25 PM (8 years, 3 months ago)

:takingnotes:
I should have improvised, I know, I'm dumb some times.
Espessially when the answer is right the fuck in front of me.
That's for the assist. I'll try being a little more crafty.
:banhamster:

Am a little drunk at the moment, and I just finished sterilizing and inoculating 5 substrate jars with a freshly made spore syringe :likeaboss:


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