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OfflineDistrict
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Wild fruit to spore print: is sterility necessary?
    #22366808 - 10/12/15 06:09 AM (8 years, 3 months ago)

I am going to take a spore print from a wild fruit.

Do I have to oven/pc sterilize the foil given that wild fruit will most likely be contaminated anyways? Or will-say- wiping with alcohol be sufficient?

I just need the ammunition so I can isolate on agar instead of going back and forth from the field.


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InvisibleLocN9ne
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Re: Wild fruit to spore print: is sterility necessary? [Re: District]
    #22366815 - 10/12/15 06:14 AM (8 years, 3 months ago)

Tear off the first 8 inches or so of the roll and save it for later... then use the foil off the roll as is... it comes sterile.


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Invisibletahoe
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Re: Wild fruit to spore print: is sterility necessary? [Re: LocN9ne]
    #22367591 - 10/12/15 10:53 AM (8 years, 3 months ago)

I wouldn't say the foil is sterile, but it's pretty dam close. Take the print on the shiny side.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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InvisibleLocN9ne
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Re: Wild fruit to spore print: is sterility necessary? [Re: tahoe]
    #22367848 - 10/12/15 11:53 AM (8 years, 3 months ago)

I ain't trying to argue with you...but aluminum foil is completely sterile due to the high temperatures during production...


--------------------


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Invisibletahoe
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Re: Wild fruit to spore print: is sterility necessary? [Re: LocN9ne]
    #22368250 - 10/12/15 01:26 PM (8 years, 3 months ago)

I ain't trying to argue but have you seen aluminum foil being made? It doesn't go from molten aluminum directly to a box on the shelf. Is the box airtight? Does it go through some type of sterilization process after its been made. It's it made in a clean room?
I agree, it's pretty dam clean. But not sterile.



--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineDistrict
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Re: Wild fruit to spore print: is sterility necessary? [Re: tahoe]
    #22370088 - 10/12/15 07:24 PM (8 years, 3 months ago)

The question is not whether the foil is sterile. The question has to do with taking spore prints from a wild mushroom - which will be contaminated anyways-- and printing on foil. I don't see why foil should go an extra step since ill be dealing with contamination anyways... unless there's something I don't see.

I need the spore prints as ammo. I have agar and can isolate.


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OfflinecronicrFacebook
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Re: Wild fruit to spore print: is sterility necessary? [Re: District]
    #22370134 - 10/12/15 07:33 PM (8 years, 3 months ago)

as said rip off a bit of foil first, that's about as good as ya need it to be, i print the first one like 5 hours then move it to another one


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OfflineDistrict
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Re: Wild fruit to spore print: is sterility necessary? [Re: cronicr]
    #22370448 - 10/12/15 08:25 PM (8 years, 3 months ago)

Quote:

cronicr said:
as said rip off a bit of foil first, that's about as good as ya need it to be, i print the first one like 5 hours then move it to another one





Do I need to do in a sab? Or a little jar? 5 hours just leave the cap on or press down some?


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InvisibleInocuole
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Re: Wild fruit to spore print: is sterility necessary? [Re: District]
    #22370587 - 10/12/15 08:43 PM (8 years, 3 months ago)

Definitely don't press down unless you want to smash the non-sterile gills against the print and ruin it.  They should drop on their own.


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OfflineDistrict
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Re: Wild fruit to spore print: is sterility necessary? [Re: Inocuole]
    #22371137 - 10/12/15 10:26 PM (8 years, 3 months ago)

Quote:

Inocuole said:
Definitely don't press down unless you want to smash the non-sterile gills against the print and ruin it.  They should drop on their own.




It's a wild fruit. It will be non-sterile to begin with.


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InvisibleInocuole
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Re: Wild fruit to spore print: is sterility necessary? [Re: District]
    #22371153 - 10/12/15 10:30 PM (8 years, 3 months ago)

It sounds like you're using that as a reason to not take any kinds of precautions, which is not how you reduce the chance of contamination.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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Invisibletahoe
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Re: Wild fruit to spore print: is sterility necessary? [Re: Inocuole]
    #22371295 - 10/12/15 11:05 PM (8 years, 3 months ago)

You will be surprised how clean wild prints are. Pushing it down and smearing it could possibly make the print dirtier. I have made what have turned out to be clean enough for grain spore syringes from wild print.
I currently have a few azure spore syringes that produced this on agar and grain.


It wouldn't hurt to make a spore syringe and try it on a few jars. I would say better than 50/50 odds of having grain spawn that is good enough for starting an outdoor patch.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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InvisibleInocuole
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Re: Wild fruit to spore print: is sterility necessary? [Re: tahoe]
    #22371654 - 10/13/15 01:52 AM (8 years, 3 months ago)

^ That's what I'm saying.  The print might be halfway clean if you take it properly, definitely don't want to smudge it up and make sure it's dirty.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineDistrict
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Re: Wild fruit to spore print: is sterility necessary? [Re: Inocuole]
    #22371849 - 10/13/15 05:15 AM (8 years, 3 months ago)

Quote:

Inocuole said:
It sounds like you're using that as a reason to not take any kinds of precautions, which is not how you reduce the chance of contamination.




I'm on my 8th try. All of which had gotten down with bacteria. I presume its from the excess water, especially now that its rainy season. The field is a soggy mess. 

How do I extract out as much of the water as possible? desiccant?


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Invisibletahoe
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Re: Wild fruit to spore print: is sterility necessary? [Re: District]
    #22372639 - 10/13/15 10:41 AM (8 years, 3 months ago)

I have dozens of wild cyan prints that are much cleaner than what you are dealing with. Been taking care of this patch for 5 years.



--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleblindingleaf
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Re: Wild fruit to spore print: is sterility necessary? [Re: tahoe]
    #22373129 - 10/13/15 12:27 PM (8 years, 3 months ago)

Quote:

tahoe said:
I have dozens of wild cyan prints that are much cleaner than what you are dealing with. Been taking care of this patch for 5 years.






:mindblown:

fuckin A dude!!  right on


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InvisibleMad Season
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Re: Wild fruit to spore print: is sterility necessary? [Re: blindingleaf]
    #22373238 - 10/13/15 12:55 PM (8 years, 3 months ago)

When I print I like to put the cap on foil in my printing box (my old small ass sab). Print for 24 hours, then let it sit open with no cap for another 24-36 hours to dry the prints out. Haven't had much problems.. :shrug:


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Invisiblejbaby007
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Re: Wild fruit to spore print: is sterility necessary? [Re: tahoe]
    #22374351 - 10/13/15 04:50 PM (8 years, 3 months ago)

Quote:

tahoe said:
I have dozens of wild cyan prints that are much cleaner than what you are dealing with. Been taking care of this patch for 5 years.





:sploosh:


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Invisibletahoe
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Re: Wild fruit to spore print: is sterility necessary? [Re: jbaby007]
    #22374826 - 10/13/15 06:13 PM (8 years, 3 months ago)

I use the short tubs for taking spore prints. Our a cookie sheet. A sheet of foil fits perfectly. Then I cover with the lid of saran wrap


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineDistrict
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Re: Wild fruit to spore print: is sterility necessary? [Re: tahoe]
    #22375030 - 10/13/15 06:48 PM (8 years, 3 months ago)

Quote:

tahoe said:
I have dozens of wild cyan prints that are much cleaner than what you are dealing with. Been taking care of this patch for 5 years.







American cyan looks very different than Asian cyan.


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