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Online99.99
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Help me understand UV light
    #22366416 - 10/12/15 12:59 AM (8 years, 3 months ago)

What will it kill in half hour exposure periods and what won't it ?
I'm looking at useing one in me heppa hood , can I just turn on the UV and the hood put all my tools in it and expect it to be steril in half hour ore is it more like a useful aid and I need to use sterile technique's to be successful


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Invisiblemustangbob3
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Re: Help me understand UV light [Re: 99.99]
    #22366483 - 10/12/15 01:31 AM (8 years, 3 months ago)

think uv sterilizer is for tools  or hard surface sterilizing.

like if was a beautician and wanted sterilze some tool.

may be able use to do petris before use? maybe

and will only kill where the light touches.
needs no shadows and 360 degree light

thought about 1 before but not sure how good and what exactly it kills.
for tools ect i sterilze in a baby bottle steam sterilizer only takes few mins for the cycle and works great.


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OfflineMrcloudy
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Re: Help me understand UV light [Re: mustangbob3]
    #22381532 - 10/15/15 12:09 AM (8 years, 3 months ago)



I just installed one in my flowhood. I did a bit of research and as best as I can tell it will kill 90% bacteria within 10 seconds and most molds within 1-30 minutes, I don't know if this applies to spores or not.

I added it for the extra protection and I plan to test it out to see if I can reuse petri dishes. I also plan on using it to possibly induce mutations in some of my cultures.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Online99.99
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Re: Help me understand UV light [Re: Mrcloudy]
    #22381739 - 10/15/15 01:44 AM (8 years, 3 months ago)

Quote:

Mrcloudy said:


I just installed one in my flowhood. I did a bit of research and as best as I can tell it will kill 90% bacteria within 10 seconds and most molds within 1-30 minutes, I don't know if this applies to spores or not.

I added it for the extra protection and I plan to test it out to see if I can reuse petri dishes. I also plan on using it to possibly induce mutations in some of my cultures.




Perfect just the info I wanted ... Do you know if the UV light will penetrate your plastic barrier I'm wondering how detrimental it is to you ??
I'm sure it can't be safe to be exposed to for long


Edited by 99.99 (10/15/15 01:55 AM)


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Invisiblemustangbob3
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Re: Help me understand UV light [Re: 99.99]
    #22381808 - 10/15/15 02:30 AM (8 years, 3 months ago)

yes bad for your eyes and skin.
when using the light stay away from it.

is the bulb uva or uvb?

would love to know what molds and bac it kills exactly.

i know some molds and bac can survive in the air at very high altitude with high uv and survive.

also the idea of uv for mutations is 1 i have heard of before as a suspected cause of albinism , would love see what you come up with.:thumbup:

if was me i would use the light for petris:thumbup:
and maybe scalpel ect

or a short bursts on wild prints to clean a little bac and hopefully not damage the spores too much.

interesting anyway...


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Online99.99
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Re: Help me understand UV light [Re: mustangbob3]
    #22381879 - 10/15/15 03:44 AM (8 years, 3 months ago)

The one I bought says UV-C , about 40 bucks off eBay


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OfflineMrcloudy
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Re: Help me understand UV light [Re: 99.99]
    #22382313 - 10/15/15 07:53 AM (8 years, 3 months ago)

Mine is UVC the plastic is a type of plexiglass, most types of plexiglass block all or nearly all UVC. Still I don't stick around in front of it when it is working.

I have read it can cause sunburn if it hits your skin, and make your eyes feel like sand paper. I am out of town but when I get back I can link a PDF with a few of the bacteria and mold species in it. I think I have a longer list somewhere but I am not sure. I will be doing my own experiments to see how long Ganoderma spores can survive being irradiated.

Ganoderma spores are notoriously difficult to germinate, but a friend of mine suspects that sterile spores germinate easier so irradiating them with UV might be a method to help it along a bit.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Invisiblemicro
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Re: Help me understand UV light [Re: mustangbob3]
    #22385581 - 10/15/15 08:54 PM (8 years, 3 months ago)

Quote:

mustangbob3 said:
yes bad for your eyes and skin.
when using the light stay away from it.

is the bulb uva or uvb?

would love to know what molds and bac it kills exactly.




UVC.

Re: plexiglass -- I wasn't sure so I Googled it:

Quote:

Only a small portion of light in the UVC region (200 – 280 nm) is transmitted




So I would check the specific wavelength but you are likely okay.

re: petri dishes I know from an article on curing skin infections it was shown to kill regular bacteria in the order of 97% in about a minute. That is not for fungal cells and I would imagine it would be more in order to pierce the chitin cell walls. The same would go for endospores.

Re: The thread title, there is a free article on Pubmed about it:

http://www.ncbi.nlm.nih.gov/books/NBK9900/

It is also used to "break apart" DNA and RNA, the latter of which is more sensitive, also to a common enzyme found almost everywhere that will destroy what you are working with.

Sunburns however, are actually from an immune response triggered by TNFα to indicate something is wrong so it would fall under the umbrella term of inflammation.


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InvisiblebodhisattaMDiscordReddit
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Re: Help me understand UV light [Re: micro]
    #22396928 - 10/18/15 10:12 AM (8 years, 3 months ago)

Miller brewing used to use uv lights with their lab hoods but has long since stopped. Their internal research concluded that they did just fine before having them and that after installing them their shit was still just fine.

Yeast work like our fungi work doesnt require ultra fancy shit just good techniques


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Invisiblemicro
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Re: Help me understand UV light [Re: bodhisatta]
    #22397776 - 10/18/15 01:33 PM (8 years, 3 months ago)

sounds about right

bleach is for laminar flow hoods (2% commercial variety)

UVC is for RNA hoods

Though it would work in a SAB except your own body is such a huge vector for contamination


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Any research paper or book for free
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OfflineCraterBait
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Re: Help me understand UV light [Re: 99.99]
    #22398361 - 10/18/15 03:37 PM (8 years, 3 months ago)

I use them, I've got one integrates into an air purification system, and another for surface/tool sterilization. They are handy to sterilize agar plates and utensils. I use uv-c at 254.7nm. This wavelength is immediately damaging to DNA amd decomposes many plastics over time so definitely be wise with it.


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