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OfflineLeeper
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Best temperature/humidity for psilocybe cubenis mycelium growth?
    #22365181 - 10/11/15 07:49 PM (8 years, 3 months ago)

I was on a mushroom cultivation thread and I mentioned temp when talking about the appearance of an orange substance on my mycelium after a couple weeks of incubation.
A few said that my temp was too high or that my moisture level was too low, or possibly both? Or contams? Any ideas? One person said it was possibly metabolites from stress because of high heat and low humidity.
I don't have any pics... On the mushroom cultivation thread somebody posted a pic that was extremely similar to this orange substance. I'm new to shroomery, but I think you can just search for my name "Leeper" and find the thread that way!
And if it is temp and humidity and not contams what is a good temp that people have found to keep contams to a minimum and mycelium growth at maximum? Thanks for the help! :smile:


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OfflineBozko
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Leeper]
    #22365229 - 10/11/15 08:00 PM (8 years, 3 months ago)

If I remember correctly, Stamets says 85 degrees fahrenheit for optimum cubensis mycelium growth, RR says up to 81. IMO, room temperature is fine enough. 

If this "orange substance" was runny and yellowish, it very well could be metabolites caused by stress.


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OfflineLeeper
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Bozko]
    #22365470 - 10/11/15 08:55 PM (8 years, 3 months ago)

I was keeping it in the range of 79-85 degrees, should I lower it? It didn't look runny, it was inside of the mycelium, almost like a bruise but not blue. Definitely an orange color. Just incubated 18 new jars, should be maybe a week before I start seeing growth! I'll keep it at 76-79 degrees F and keep posting on progress. What about humidity?


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OfflineBozko
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Leeper]
    #22365504 - 10/11/15 08:59 PM (8 years, 3 months ago)

90% percent humidity for spawn run, according to The Mushroom Cultivator by Stamets.


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ShadeOfDeepPurple said: I guess you don't get shamanism yet by the very fact that you describe a psychedelic as Mexican.


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InvisibleJacobStorm
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Bozko]
    #22366755 - 10/12/15 05:19 AM (8 years, 3 months ago)

Bacteria loves heat. Incubate between 70-75 degrees. Fruit at room temp. But perfect fruiting temperature is 75 degrees.


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InvisibleMad Season
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: JacobStorm]
    #22367539 - 10/12/15 10:39 AM (8 years, 3 months ago)

Quote:

RogerRabbit said:
It was all posted on the other board.  I don't know if the threads are still there or not.  Bottom line was the tubs that had petri dishes between 75F and 81F showed no difference in growth.  Below 75F, and above 81F growth slowed down, with a rapid drop in colonization speed below 70F and above 83F.  At 86F, a petri dish would be 2/3 colonized, while its sister at 75F would be fully colonized.  Rate of growth at 86F was exactly the same as rate of growth at 72F, with fastest growth as said, occurring between 75F and 81F.

Note that these tests were for linear growth in the two dimensional plane of a petri dish.  In three dimensonal space such as in grain jars or bulk substrates, the effects of thermogenesis need to be considered, so ambient temps should be lowered slightly to compensate.
RR




70-75 is the best for colonisation.

During colonisation there is no need to worry about humidity. It will have a filter for gas exchange that'll hold humidity down.

As for fruiting.. You want 99% SURFACE HUMIDITY. RELATIVE HUMIDITY ISNT IMPORTANT.

As seen here: http://www.shroomery.org/forums/remlinker.php?Cat=&Entry=149412&F_Board=2&Thread=21288129&Main=21288129

http://www.shroomery.org/forums/showflat.php/Number/22105120

Quote:

The thing to remember(which is something i find misunderstood) is that the most important humidity you have is surface humidity, in other words it really doesn't matter if your fc has a rh of 40% or 99% as long as there is room for evaporation to take place the humidity that is important sits on the surface.

This is our goal with every fruiting chamber we use is surface humidity, with our monotubs we dial them in to achieve this, with our sgfc's we mist as needed to achieve this, with our gh's we (depending on your set up)use something like an ultrasonic humidifier as a timed mister to achieve this because once we have this we can focus on fae which is a great tool for everything your substrate wants for many reasons, the more the better:super:

The reason we grow in a high humidity environment is simple, mushrooms have no skin and lose moisture to the environment very easily and if they lose more then is being pumped in they simply die. This is an easy fix just simply misting your fruits




There's also this, which is a good explanation:
Quote:

Air exchange is the number one pinning trigger.  Humidity is farther down the list.  Perhaps the number 2 pinning trigger is a loss of moisture from the substrate.  If you try to avoid misting by constantly pumping in wet air, you don't have a loss of moisture from the substrate and performance is poor.

You want constant air exchange, and then as the cake or other substrate dries out, you mist to replace the lost moisture.  This dry-wet cycle is a MAJOR pinning trigger. There is no automated system that can replace your eyeballs and mister.  I'm an electrical engineer by profession with a specialty in automation, so believe me when I say if the whole process could be automated, I'd have done it years ago.
RR




And last but not least what proper misting looks like: http://www.shroomery.org/forums/showflat.php/Number/21595437


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Edited by Mad Season (10/12/15 10:43 AM)


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InvisibleMad Season
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Mad Season]
    #22367756 - 10/12/15 11:35 AM (8 years, 3 months ago)

As for helping you op.. You must provide pics. The world of molds is a vast one. Its impossible to say anything without pics. All I can do is do what I did. Which is a generalization of humidity, temperature, and fae.

During colonisation there's no such thing as too much or not enough humidity. But there is such a thing as too wet or too dry. This is determined by the amount of water you put in when you prep, and how well you mixed it up. As they say, always ajm for stuff on the dry side. Much better for colonisation.


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (10/12/15 11:36 AM)


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OfflineLeeper
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Mad Season]
    #22374857 - 10/13/15 06:18 PM (8 years, 3 months ago)

Awesome, I'll lower the temp! Thanks guys!!! :smile:


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OfflineHandslikehouses
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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Leeper]
    #22408260 - 10/20/15 09:06 AM (8 years, 3 months ago)

I colonize and fruit at 75. Works fuckin great every time.


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Re: Best temperature/humidity for psilocybe cubenis mycelium growth? [Re: Bozko]
    #23674710 - 09/24/16 05:39 PM (7 years, 4 months ago)

Quote:

Bozko said:
If I remember correctly, Stamets says 85 degrees fahrenheit for optimum cubensis mycelium growth, RR says up to 81. IMO, room temperature is fine enough. 

If this "orange substance" was runny and yellowish, it very well could be metabolites caused by stress.




The Cosmos! Nice sig, bruh.


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