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OfflineMoldy thumb
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First time Agar grow log
    #22363510 - 10/11/15 02:10 PM (8 years, 3 months ago)

I am starting this as a log for my second grow and first time working with Agar. I will be going from Agar to lc then to DVG  spawn bags. I started this log a little late so I don't have pictures of the original Agar that I inoculated. Here are the second generation jars

Golden teacher


Ecuador


I plan to take 2 of the best looking points from these dishes and transfer them to Lc.

Any input is welcomed so please chime in if you see me doing anything wrong.


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Edited by Moldy thumb (10/11/15 02:14 PM)


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InvisibleShroomspective
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Re: First time Agar grow log [Re: Moldy thumb]
    #22364559 - 10/11/15 05:39 PM (8 years, 3 months ago)

Looking great so far :thumbup:


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OfflineMysticMoteToter
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Re: First time Agar grow log [Re: Moldy thumb]
    #22365003 - 10/11/15 07:07 PM (8 years, 3 months ago)

I'm going to be trying agar soon so i'm  :takingnotes:


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OfflineMoldy thumb
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Re: First time Agar grow log [Re: MysticMoteToter]
    #22365114 - 10/11/15 07:30 PM (8 years, 3 months ago)

Sad day, I found contams in both jars earlier today :/ each Agar jar I've made so far has had at least one bacterial spot in it, hopefully another transfer will fix it up



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OfflinePsilosoulful

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Re: First time Agar grow log [Re: Moldy thumb]
    #22365340 - 10/11/15 08:28 PM (8 years, 3 months ago)

That sucks dude. You did all the work in the SAB right?

The better your sterile techniques, the less chance of serious contams.

Check this tek out, it goes step by step with pics, it helped me out a lot!! http://www.shroomery.org/forums/showflat.php/Number/21889950/fpart/1/vc/1


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InvisibleLocN9ne
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Re: First time Agar grow log [Re: Psilosoulful]
    #22365364 - 10/11/15 08:32 PM (8 years, 3 months ago)

Check the "condition" of your SAB...blow a smoke ring in it... then put your hands in and go through the motions you would if doing a transfer and see how much your movement disrupts the air... it's a good visual aide...and dry runs really help with getting the feel for your SAB, and strengthen your technique. There's definitely a method to the madness...


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Edited by LocN9ne (10/11/15 08:34 PM)


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OfflineMoldy thumb
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Re: First time Agar grow log [Re: LocN9ne]
    #22365400 - 10/11/15 08:41 PM (8 years, 3 months ago)

Psilosoulful thanks for the link, tons of good info in there.  I'll definitely check the SAB locN9ne. Tomorrow after work I'll check the box then do another transfer.


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OfflinePsilosoulful

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Re: First time Agar grow log [Re: Moldy thumb]
    #22365934 - 10/11/15 10:08 PM (8 years, 3 months ago)

:rockon:


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OfflineMoldy thumb
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Re: First time Agar grow log [Re: Psilosoulful]
    #22371021 - 10/12/15 09:54 PM (8 years, 3 months ago)

Made some more transfers today and 2 LC's.  I'll post some more pictures in a day or 2 to show progress. Also took a look at my sterile techniques and made a few tweaks. Hopefully it cuts down on my bacterial contams.


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OfflinePsilosoulful

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Re: First time Agar grow log [Re: Moldy thumb]
    #22371313 - 10/12/15 11:10 PM (8 years, 3 months ago)

Nice SAB. The foil is a bit unnecessary tho, you're gonna be moving your hands in and out of there anyway. Doesn't really serve much of a purpose. Just make sure to spray the inside with soapy water, let it settle, then work slowly, no fast movements. Most importantly, flame sterilize after each plate and don't let your hand hover over any exposed plates. I found Mad Season's write-up on sterile techniques to be quite helpful: http://www.shroomery.org/forums/showflat.php/Number/21889950/fpart/1/vc/1


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OfflineMoldy thumb
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Re: First time Agar grow log [Re: Psilosoulful]
    #22371866 - 10/13/15 05:24 AM (8 years, 3 months ago)

That was very helpful to me as well. I know it's probably not necessary but hey its better to err on the side of caution than be sloppy right?


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OfflinePsilosoulful

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Re: First time Agar grow log [Re: Moldy thumb]
    #22372087 - 10/13/15 08:07 AM (8 years, 3 months ago)

Yeah that's fine. :thumbup:

Here's my SAB. It's got the tyvek sleeves on the side with a small flap over it. As well as a hole on the side for moving my tools inside and out easily.



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OfflineMoldy thumb
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Re: First time Agar grow log [Re: Psilosoulful]
    #22372380 - 10/13/15 09:35 AM (8 years, 3 months ago)

Nice, I might have to try that with the tyvek sleeves


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OfflineMoldy thumb
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Re: First time Agar grow log [Re: Moldy thumb]
    #22374909 - 10/13/15 06:26 PM (8 years, 3 months ago)

So I came home and check my Agar culture es today and found that they are once again bacterial.



I set out my unused plates to warm up. Kind of a long shot but maybe, just maybe they didn't get completely sterilized


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OfflinePsilosoulful

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Re: First time Agar grow log [Re: Moldy thumb]
    #22374973 - 10/13/15 06:37 PM (8 years, 3 months ago)

That sucks dude :sad:

How long did you PC them for?


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OfflineMoldy thumb
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Re: First time Agar grow log [Re: Psilosoulful]
    #22375007 - 10/13/15 06:43 PM (8 years, 3 months ago)

30 minutes but I definitely didn't bring the heat up slowly like some of the teks say to do.


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OfflinePsilosoulful

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Re: First time Agar grow log [Re: Moldy thumb]
    #22375069 - 10/13/15 06:56 PM (8 years, 3 months ago)

Make sure it reaches 15 psi before lowering the heat to medium. Then, start your timer for 30 minutes.

Bringing up the heat slowly will only slow down the process. I turn my stove to the highest setting after loading my PC.

When your 30 minutes is up, then slowly lower your temp in order to reduce condensation on the sides of your jar. It's worked well for me so far.:thumbup:


Edited by Psilosoulful (10/13/15 06:58 PM)


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