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InvisibleLizardWizard
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Agar plates, contammed or healthy growth?
    #22362557 - 10/11/15 10:24 AM (8 years, 3 months ago)

Hi y'all!

So I had an LC from Peruvian Ps.C. spores (sporeprint), and squirted some of it on a petri. Got clean growth. I also started the LC on a load of Millet grains. Here's the culture in fruiting stage:


Now I also started some agar plates from the same print. Saw first growth after about a month. Here's where it gets weird..

that started out white, then went to greyish, and then spread on in white, but with the brown orange side... Contam??


This one got very light fluffy growth, made me wonder whether it was really mushroom mycelium..? (the white hue in top centre is condensed water)



And this one from the original LC grown out on a petri, and then transferred on another. I got some contamination, but it started out in the red circle, got overgrown by the mycelium, and then I saw it had also contammed on the sides - seemingly unconnected. My question here is: When the mycelium overgrows a contam, you don't see it anymore on the agar plate. Does it still live? Has the mycelium eaten the contam? Is it safe to use that spot to transfer to grains? I would test it out, but I'm not confident enough about my sterile technique yet to give any kind of exclusion of foreign contaminants outside that one, if you catch my drift. Might try though...

Thanks!


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InvisibleMad Season
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Re: Agar plates, contammed or healthy growth? [Re: LizardWizard]
    #22367496 - 10/12/15 10:27 AM (8 years, 3 months ago)

Give this thread a thorough read. It has all your questions answers

http://www.shroomery.org/forums/showflat.php/Number/22020260

Basically I wouldn't trust those plates


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