Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Original Sensible Seeds High THC Strains

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineFungus_Side
Stranger and stranger
Registered: 10/11/15
Posts: 35
Loc: Wait, what?
Last seen: 4 years, 4 months
Spore density
    #22361550 - 10/11/15 01:29 AM (8 years, 3 months ago)

First time with mushrooms, but thought it might be best here.

Should the density of spores in a syringe be high enough to allow some growth on Sabouraud medium with just 3-4 drops? I'm not seeing hyphae in my jars (PF Tek style), nor on the plates with Sabouraud dextrose that I had lying around, and am wondering if the syringes were bad or if the density of spores is just that low.


Extras: Filter Print Post Top
Offlineforrest
 User Gallery


Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
Last seen: 4 years, 6 months
Re: Spore density [Re: Fungus_Side]
    #22361653 - 10/11/15 02:49 AM (8 years, 3 months ago)

how long ago did you inoculate?


--------------------
My Trade List


Extras: Filter Print Post Top
OfflineFungus_Side
Stranger and stranger
Registered: 10/11/15
Posts: 35
Loc: Wait, what?
Last seen: 4 years, 4 months
Re: Spore density [Re: forrest]
    #22365219 - 10/11/15 07:57 PM (8 years, 3 months ago)

13 days ago. No signs of growth in either of two syringes used- different strains of cubensis. I have extensive tissue culture experience, and a graduate course in mycology many years ago, so I kinda know what I'm doing but am confused at what I see.

Now that I look at reviews, I find my suppler has had problems this year- individuals complaining their syringes did nothing. So perhaps the problem is not on my end.


Extras: Filter Print Post Top
InvisibleToadstool5
A Registered Mycophile
Male User Gallery


Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
Re: Spore density [Re: Fungus_Side]
    #22365369 - 10/11/15 08:33 PM (8 years, 3 months ago)

I would believe so. Only two are needed and even a very light syringe has hundreds if not thousands.

I personally make syringes for agar REALLY concentrated so i can use a single drop. The extra water can help bacteria contam the plates and if there are contaminates in the syringe from the print being contaminated i simply smear the drop and transfer out.

Shipping temperatures or errors on the sponser's part can cause dud syringes. Asking for a replacement doesn't seem too unreasonable.


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


Extras: Filter Print Post Top
OfflineFungus_Side
Stranger and stranger
Registered: 10/11/15
Posts: 35
Loc: Wait, what?
Last seen: 4 years, 4 months
Re: Spore density [Re: Toadstool5]
    #22387550 - 10/16/15 09:18 AM (8 years, 3 months ago)

Spores from Syzygy took 4 days to sprout on Sabouraud; still no growth from either of the spore syringes from the first vendor- plates or jars. But I now have growth in jars from other spore syringes, so- now there is some level of success!


Extras: Filter Print Post Top
InvisibleToadstool5
A Registered Mycophile
Male User Gallery


Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
Re: Spore density [Re: Fungus_Side]
    #22388648 - 10/16/15 02:29 PM (8 years, 3 months ago)

Well now you know it was the syringes and not your media or technique. That's always reassuring.

Now that you have actives growing all you need is a pleurotus species or lentinula edodes :awesomenod:


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


Extras: Filter Print Post Top
OfflineDrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
Re: Spore density [Re: Toadstool5]
    #22417683 - 10/22/15 11:05 AM (8 years, 3 months ago)

You should see some growth, maybe not at every inoculation point, if the syringe was really sparse.

If your just seeing growth now, I'd wait a while longer. Maybe boost up the temp in your room a little if its below 70F.

Is it possible that you were only squirting enough liquid to cool the syringe each time you inoculated?

Also sometimes the spores like to clump up in the syringe and a good shake is needed. Course then you would have seen some growth somewhere from these syringes.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Original Sensible Seeds High THC Strains


Similar ThreadsPosterViewsRepliesLast post
* Spore germination Mexicana A Yachaj 1,972 4 05/14/02 09:03 AM
by baraka
* Re: Contaminated spores and idea about growing WorkmanV 1,011 1 02/04/00 04:15 AM
by Crobih
* Single Strain (spore) Isolation and the Benefits.. TrippinRhino 9,131 18 07/24/01 12:02 AM
by Anno
* Innoculating MEA with syring? upupup 1,353 5 10/29/02 10:30 AM
by upupup
* Multi spore inoc?? badjessejames 1,571 5 07/23/01 05:16 AM
by Anno
* Save contaminated spores with agar ? tomldp 3,548 9 04/10/02 05:34 PM
by ParticleMan
* Growing mycelium inside a syring?advice? shrogg 1,044 1 05/13/02 08:05 AM
by EvilGir
* Spore Jar Technique (thanks to PokeSmot!) Rustik 4,480 17 05/30/03 04:16 AM
by mycophreak

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
744 topic views. 1 members, 12 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.014 seconds spending 0.004 seconds on 14 queries.