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ALiStOmega
Mr.



Registered: 10/16/11
Posts: 97
Loc: USA
Last seen: 5 years, 4 months
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What is this Called
#22360380 - 10/10/15 07:12 PM (8 years, 3 months ago) |
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I made this today because I am finally back in the game ladies and gents... what is this though the nomenclature evades me

and another closer up

I am super excited about this still for 3 years have been on the road and now I can resume where I left off!!!
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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A still-air-box or SAB? Or is there another name you are thinking of?
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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ALiStOmega
Mr.



Registered: 10/16/11
Posts: 97
Loc: USA
Last seen: 5 years, 4 months
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No that's what I was thinking too. I am so proud of this now lol only cost less than 30 dollars to make
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InfiniteToker
Devourer of Chicken Wings



Registered: 06/22/13
Posts: 1,724
Loc: Earth
Last seen: 1 month, 5 days
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Good luck!, I don't have my own place, currently renting. I would totally be in the game trying out the truffle tek I peeped on here, have fun!
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"I'm chilling in a room with a view, there's always room for improvement; so i grab my coat and go and prove it"-Method Man
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ALiStOmega
Mr.



Registered: 10/16/11
Posts: 97
Loc: USA
Last seen: 5 years, 4 months
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I am thinking of placing a fan on the back partition and HEPA filters any recommendations opinions of all sorts are welcome? At the same time I don't really see the need either, it amounts to if its better I want to build it
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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If you added a HEPA filter and fan it would be a laminar flow hood. It's not necessary if you let the air settle first but it is way easier and in my opinion better.
Matching a fan to a filter can be difficult and I have never built a flow hood so somebody else will have to help you with that. The pressure of the air flow and the seal is very important from what i have been told.
Here's a commonly circulated link with everything you need to build a laminar flow hood:
http://www.fungifun.org/English/Flowhood
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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ALiStOmega
Mr.



Registered: 10/16/11
Posts: 97
Loc: USA
Last seen: 5 years, 4 months
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Re: What is this Called** [Re: Toadstool5]
#22363980 - 10/11/15 03:49 PM (8 years, 3 months ago) |
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I added a light source now this is pimped out
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 15 hours, 9 minutes
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Re: What is this Called** [Re: ALiStOmega]
#22380634 - 10/14/15 08:13 PM (8 years, 3 months ago) |
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Technically is should be called a barrier. I use something similar though and just call it a still air box.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: What is this Called** [Re: Kizzle]
#22385669 - 10/15/15 09:09 PM (8 years, 3 months ago) |
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Out of curiosity why not just use a bunsen burner?

Your hands will bring air convections and unsterility in a SAB; if you have sterile gloves that would be a plus. If you don't use ethanol spray, but if you are using a bunsen burner obviously dry your hands before lighting it. The air will not be sterile in a SAB but it is working in a better environment. Add a UVC light and technically you have an RNA hood :V
I've been trying to get this point across for a while; it is practiced in molbio labs all the time without a laminar flow hood for doing sterile cultures.
It was also one of the first pictures to come up when I google imaged asceptic bunsen burner.
I feel proud :3
Also, not sure if I mentioned this but we used a heat sealer for the bags. This is basically indispensable or you will need to resort to the lighting-the-bag-with-a-lighter technique you used for bags of weed in cigarette cellophane like you did in high school.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
Edited by micro (10/15/15 10:19 PM)
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: What is this Called** [Re: micro]
#22386302 - 10/15/15 11:15 PM (8 years, 3 months ago) |
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Good tips micro, keeping it simple!
I would work from a SAB or laminar out of comfort and familiarity but a bunsen burner is quicker, more readily available in labs, and much more portable.
My friend's plant pathology course usually does open air using this method without any issues. They have yet to touch the laminars but they only do agar work for the most part.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 15 hours, 9 minutes
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Re: What is this Called** [Re: Toadstool5]
#22395415 - 10/17/15 09:05 PM (8 years, 3 months ago) |
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The box is mainly to keep dust, hair, skin flakes, etc from falling into the work area. It also lets me keep the work area maticulously clean since it can be sealed off when not in use to keep dust and crap out and reduce the amount of time spent cleaning. I do leave my alcohol lamp sitting on top which may offer some extra protection but at the very least setting it there keeps the convection currents from passing through the box.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: What is this Called** [Re: Kizzle]
#22397794 - 10/18/15 01:38 PM (8 years, 3 months ago) |
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Look at the picture again >.<
The air flows around in a circle, making a 10cm radius to use for sterile work.
A radius is round and implies a circle. If you put it on top it is basically doing nothing.
Actually, it becomes an ornament, kind of like a hat.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 15 hours, 9 minutes
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Re: What is this Called** [Re: micro]
#22405887 - 10/19/15 10:17 PM (8 years, 3 months ago) |
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It doesn't create a sterile sphere of air if that's what you're implying. It alters the trajectory of settling aerosol particles so they're less likely to land within a certain radius. So yes it is like a hat that reduces the number of particles that drop beneath it. In the case of a SAB the less particles drifting around the arm holes the better.
The picture is a little misleading. The sterile stream of air above the flame does not stay sterile as it moves along with the convection currents, it mixes with non-sterile air. The benefit comes from the tendency of particle to move in direction the air is moving.
In other words if you were leave it burning for a long time you'd find a lack of dust beneath it. You wouldn't find that the dust is sterile.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: What is this Called** [Re: Kizzle]
#22406833 - 10/20/15 12:15 AM (8 years, 3 months ago) |
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how am i going to explain this...
Ah! I'm not. Cause I really don't care at this point.
Nice hat! :V
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Gr0wer
always improving


Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
Last seen: 5 years, 10 months
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Re: What is this Called** [Re: micro]
#22416206 - 10/21/15 11:39 PM (8 years, 3 months ago) |
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My head huts every time i come to "advanced mycology"
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: What is this Called** [Re: Gr0wer]
#22418326 - 10/22/15 01:26 PM (8 years, 3 months ago) |
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Quote:
Gr0wer said: My head huts
You know there is a "post" button you can use to make threads o.O
Say something intelligent. If enough people do that, it will change.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 15 hours, 9 minutes
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Re: What is this Called** [Re: micro]
#22421257 - 10/23/15 02:02 AM (8 years, 3 months ago) |
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Like I said I set it there to keep the currents from passing through the box and not specifically to protect my work, that's what the SAB is for. I don't know where you're getting this idea that a flame effectively sterilizes all the air around it, apparently from a picture, but it's not true. The majority of the air being circulated never actually passes through the flame to reach those kind of temperatures.
Quote:
Among other uses, the heat of a Bunsen burner flame can be used to create a convection current, which heats the space above the flame and lifts any particulates in the air up and away from the cooler air underneath it, keeping this work area sterile.
http://www.jove.com/science-education/5035/introduction-to-the-bunsen-burner
Quote:
A Bunsen burner provides an inexpensive and reasonable sterile field. The heat from the flame creates a convection current moving upwards around the flame. As a result, a small circumference around the burner is free of falling contaminants.
http://ind.ntou.edu.tw/~b0232/surface.html
Here's a more accurate picture
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Edited by Kizzle (10/23/15 03:28 AM)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: What is this Called** [Re: micro]
#22422486 - 10/23/15 11:09 AM (8 years, 3 months ago) |
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Quote:
micro said:

how am i going to explain this...
Ah! I'm not. Cause I really don't care at this point.
Nice hat! :V
i.e. do your own research and please drop this interminable fucking nonsense
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: What is this Called** [Re: micro]
#22545379 - 11/19/15 06:42 AM (8 years, 2 months ago) |
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I'm taking a stab at this. I have a cheap bunsen burner and the flame is not effected at all when I open and close the air vent, it's just bright yellow with almost no blue flame. I don't know what the problem is there.
I wanted to try and get an idea about the air currents so I lit a stick and blew out the flame to create smoke and placed it near the flame, the smoke wanted to go virtually straight up (about 8 feet up) every time to the ceiling where it dissipated and I could no longer see it. I was hoping maybe I could see the smoke moving in circles but that was not the case. The flame was about 3 inches tall the first time and 6 inches the second time.
I want to set up an experiment to test the sterility of the air by placing agar slants at certain intervals from the flame, I have 6 slants I thought I should place each one a little further from the flame, sterilize the caps with flame then open each one and leave them open for a bit, 15 minutes?

Suggestions?
Edited by invitro (11/19/15 06:44 AM)
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: What is this Called** [Re: invitro]
#22545390 - 11/19/15 06:50 AM (8 years, 2 months ago) |
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The bunsen burner is about 2.5 or 3 inches tall, it's the cheapest one I could order online "forbes" brand. If you hold a flame to the air vent you get a little flame coming out of it. So instead of taking air in, it's actually letting gas out, not ideal. Anyhow I don't think that should effect my results.
All the amazon review comments on different types of burners report yellow flames when using LP, so maybe my sitation is more normal than I thought.
Edited by invitro (11/19/15 06:59 AM)
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