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dynamitehack
Stranger
Registered: 09/11/15
Posts: 20
Last seen: 8 years, 25 days
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First Time Grower Questions
#22358528 - 10/10/15 10:43 AM (8 years, 3 months ago) |
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I'm such a noob, I apologize in advance.
My "guide" was a youtube video, its old school documentary style.
Problem 1. I bought prints instead of syringes on accident, but I was able to go to Canada to get some syringes. I used the one print to make 5 syringes and used 4 of those on 12 jars. They're only 3mL. I was SURE I was going to mess something up and things wouldn't be kept sterile.
Problem 2. I punched the holes in the lids going the opposite way so that they ripped holes in the tin foil while pressure cooking.
*****Problem 2.5. I only made 2 holes for each jar instead of 4.
Problem 3. I first put the inoculated jars in a container, in a garbage bag, and in the cupboard above the fridge. I left the foil on. After like 4 days, I noticed that inside the jars was so incredibly wet. I took the foil out, and moved the container (clear) to just sitting on top of the fridge. Which doesn't put out any heat. But I don't know if this is the right thing to do.
Problem 4. After 4 weeks, only about half of the jars had the bottom colonized. One day I was checking and noticed pinheads and really screwed up. I immediately took them out and put them in the terrarium. Even a few that weren't fully colonized. The ones that weren't colonized, I scraped off the extra substrate and they seem to be doing fine.
I haven't had ANY contamination even though I wasn't all that careful. I wore gloves but I didn't wipe with alcohol in between injections and I forgot to flame the syringes a few times too.
What is all this rinsing and dunking I hear about? I have a few jars that are totally stagnant and not colonizing any further. Should I just take them out and see what they produce? What else am I doing wrong?
THANKS
Edited by dynamitehack (10/10/15 10:47 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341
Just... start over. If you're birthing uncolonized cakes, and everything started fruiting before it was fully colonized, you probably have contamination that you can't see yet. How did you even punch the lids the other way? And why?
Wiping syringes with alcohol is dumb anyway, good that you didn't do that.
Cakes that weren't dunked and rolled will probably not perform as well as if they had been.
Dunno what else to say other than read.
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dynamitehack
Stranger
Registered: 09/11/15
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Re: First Time Grower Questions [Re: Inocuole]
#22358929 - 10/10/15 12:16 PM (8 years, 3 months ago) |
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Can I still dunk them after the first flush? Also, is it possible that I just didn't inject the spores deep enough?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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You can still dunk after the first flush, you just can't roll them in verm like you can right after birthing. Not sure what you mean about not injecting spores deep enough, the whole cake should colonize no matter where you injected it, just takes longer from some positions.
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shroombasa
Sojourner



Registered: 05/02/15
Posts: 364
Loc: 'Merka
Last seen: 3 years, 3 months
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Quote:
dynamitehack said: I'm such a noob, I apologize in advance.
My "guide" was a youtube video, its old school documentary style.
Try RR's videos. They're probably the second best resource in this hobby second to Paul Stamet's books.
Problem 1. I bought prints instead of syringes on accident, but I was able to go to Canada to get some syringes. I used the one print to make 5 syringes and used 4 of those on 12 jars. They're only 3mL. I was SURE I was going to mess something up and things wouldn't be kept sterile.
IMO, prints are always a crapshoot because you probably have contams in there that you can't see. Even if you used a glovebox or flowhood to convert your print to a syringe and followed sterile procedure to a T, I'd be willing to be you've got uninvited guests in there.
Problem 2. I punched the holes in the lids going the opposite way so that they ripped holes in the tin foil while pressure cooking.
*****Problem 2.5. I only made 2 holes for each jar instead of 4.
That shouldn't be much of an issue depending on the size of your holes. I only punch two holes in mine and never experience GE problems.
Problem 3. I first put the inoculated jars in a container, in a garbage bag, and in the cupboard above the fridge. I left the foil on. After like 4 days, I noticed that inside the jars was so incredibly wet. I took the foil out, and moved the container (clear) to just sitting on top of the fridge. Which doesn't put out any heat. But I don't know if this is the right thing to do.
As far as the trash bag and foil, you should remove both. Your spawn needs GE. Just place the jars on a shelf at room temperature.
Problem 4. After 4 weeks, only about half of the jars had the bottom colonized. One day I was checking and noticed pinheads and really screwed up. I immediately took them out and put them in the terrarium. Even a few that weren't fully colonized. The ones that weren't colonized, I scraped off the extra substrate and they seem to be doing fine.
I've done this once. It worked, but it's certainly not ideal. The cake only fruited once before contaminating.
I haven't had ANY contamination even though I wasn't all that careful. I wore gloves but I didn't wipe with alcohol in between injections and I forgot to flame the syringes a few times too.
If you're using MS and it took 4 weeks for some of your jars to only partially colonize, I'm guessing there are probably contaminants in your grow. Contamination doesn't mean your grow will just keel over and die. The mycelium can still colonize and you can still get fruits in some cases.
What is all this rinsing and dunking I hear about? I have a few jars that are totally stagnant and not colonizing any further. Should I just take them out and see what they produce? What else am I doing wrong?
THANKS
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Edited by shroombasa (10/10/15 02:53 PM)
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DrCrumbs
Alpha Blue


Registered: 10/25/11
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Re: First Time Grower Questions [Re: Inocuole]
#22359535 - 10/10/15 02:49 PM (8 years, 3 months ago) |
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Sounds like you'll still get something. Also awesome no contams.
But yeah start another batch, you've learned a lot already.
Is it possible that they stalled because your brf/verm mix was to wet and you packed it in? (shouldn't look wet, and load your jars light and fluffy the myc will hold it all together)
Let a bead of spore water run down each side.
When my PC'ing is done I place them on paper towels while I'm shooting them up with spores/LC/etc. Then they go into a tote with a clean towel, or paper towels in the bottom. Water on the outside can cause problems. Sometimes the water can have nutrients from the cook which can mold.
I would have been scared to dunk after you birthed since you would have had small parts of uncolonized cake that could have easily contam'ed.
After the first flush dunking should be fine.
Edited by DrCrumbs (10/10/15 02:54 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: First Time Grower Questions [Re: shroombasa]
#22359545 - 10/10/15 02:51 PM (8 years, 3 months ago) |
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The verm roll is absolutely not for warding off contams. Seriously? It's a casing layer for the cake, to retain moisture and help promote a good pinning surface. 
Doing it after the first flush will trap all the contams that have fallen on it over the course of a week or two against the surface and germinate them all.
How is dunking an uncolonized cake any worse than putting it straight in a fruiting chamber? At least you'll rinse it off with the dunk. (although you should be rinsing the hell out of it anyway) The dunk helps the verm stick to the cake during the roll.
God damn, you guys...
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shroombasa
Sojourner



Registered: 05/02/15
Posts: 364
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Re: First Time Grower Questions [Re: Inocuole]
#22359562 - 10/10/15 02:56 PM (8 years, 3 months ago) |
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Same idea as the verm barrier, no? And I reread that after posting and realized I mispoke.
-------------------- Trade list
Peace and long life.
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DrCrumbs
Alpha Blue


Registered: 10/25/11
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Re: First Time Grower Questions [Re: Inocuole]
#22359565 - 10/10/15 02:57 PM (8 years, 3 months ago) |
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You would be bathing them in a non sterile nute bath.
This board has gotten a little more hostile than I remember.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: First Time Grower Questions [Re: shroombasa]
#22359572 - 10/10/15 02:59 PM (8 years, 3 months ago) |
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Quote:
shroombasa said: Same idea as the verm barrier, no?
Not even remotely. The dry verm barrier is a natural filter during colonization. If it gets wet it's ruined. The verm that you roll in after the dunk is a thin casing layer on the surface of the cake. It's supposed to remain moist, it helps create humidity pockets on the surface which is where pins form. Otherwise you get them all fruiting from the bottom where the cake is touching the foil.
Quote:
DrCrumbs said: You would be bathing them in a non sterile nute bath.
This board has gotten a little more hostile than I remember.
Uhh, not if you rinsed them first. If it's a non-sterile nute bath when you dunk it, what is it when you put it in a fruiting chamber and mist it? Something different then?
Hostile.
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dynamitehack
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Last seen: 8 years, 25 days
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Re: First Time Grower Questions [Re: Inocuole]
#22362205 - 10/11/15 08:54 AM (8 years, 3 months ago) |
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Thanks guys, you've all been incredibly helpful. I attached a picture of what they look like right now. Is this normal? What happens if they're growing down into the perlite?
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eagle_eye6977
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Registered: 08/24/14
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hey everyone! im having a problem with my lc's not growing. I have a mono-culture of b+ that i have successfully isolated and fruited. I isolated on agar through many transfers. I test fruited with 4 flushes. My lc's look healthy with massive amounts of rhizomorphic growth. They were transfers from the test fruited plates. My question is why are they not growing now on agar or grain that they are in liquid suspension? any feedback would be greatly appreciated. thanks in advance.
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aerow.thefox
professional amatuer



Registered: 06/10/10
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Quote:
dynamitehack said: Thanks guys, you've all been incredibly helpful. I attached a picture of what they look like right now. Is this normal? What happens if they're growing down into the perlite?
you need some jar lids or foil under those. its not terribley bad but its better. easier cleanup in my opinion too. i did that before thinking it was not big deal and they grew into the perlite. then i couldnt get all the organic matter out, started to stink like bacteria. had to dump it out and do a thorough clean and bake. so id recommend a barrier.
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Ive grown directly on perlite with no issues. The shrooms like the extra water.
But adding a jar lid under the jars works just as good. Likely cleaner for the cakes.
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dynamitehack
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Last seen: 8 years, 25 days
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I put jars under them all, I didn't know that was a thing either. :/
Now I woke up this morning and a few of the veils have broken, but most of the others aren't even close to ready. Is it okay if I leave them be for a few days until more are ready, and then I can start dehydrating? Or do they need to picked immediately?
Thanks again
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Inocuole
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Registered: 11/21/11
Posts: 24,863
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If you leave them after the veil breaks they shit spores all over the place and start going soft after the cap has flipped almost completely inside out.
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dynamitehack
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Re: First Time Grower Questions [Re: Inocuole]
#22383128 - 10/15/15 11:52 AM (8 years, 3 months ago) |
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I feel like I screwed everything up and failed miserable. I already ordered more spores and I can't wait to try the whole process again, fixing the mistakes I made the first time.
I've harvest the first flush off of about 7 cakes so far, the rest are slow/not doing much of anything. I've since dunked those and I'm seeing some decent pinning on 4 of those. The remainder isn't doing much.
Some of the cakes are are very bluish, but not like bruising. Like certain "strings" of the mycelium is blue.
So far I have about 15g cracker dry but I feel like that is pretty sucky.
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
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Not gonna lie for a first attempt and screw ups,you got a not so bad yield good stuff bro. Looking forward to when you do it a bit better. Keep us posted.
Edited by GreenHornThumb (10/15/15 11:59 AM)
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,359
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Dont be so hard on yourself.
For a first time ever grow, 15 grams dry is really good.
Wanna see my first flush?

Thats right, only one small mushroom. Thats all I got from my three cakes. Sad.
Be thankful for you 15 dry grams, its much better than most people grow on their first time.
And learn from your mistakes and apply the right way the next time 
You could have done WAY worst.
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dynamitehack
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Registered: 09/11/15
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Thanks guys, that definitely helps. I read a forum about others' first grow and saw that a lot failed so I'm pretty happy that I've actually grown SOMETHING.
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