Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineRo0k
Stranger

Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
When should I transfer plates?
    #22357932 - 10/10/15 07:56 AM (8 years, 3 months ago)

Hidy-ho there fellow travellers,

Quick question about when I should start doing transfers from some agar plates to work on strain isolation. 



Put way too much liquid on the above two plates last Saturday, so I thought they were a bust, but to my surprise, I"m getting some really good growth in 90% of the cases... there are 2 spots on one plate you can't see from the pic where strawberry-seed-sized bacteria of some sort is growing in one of the cultures and just next to it, so that area is a bust (it's yellowish-red).  It's at the 11 o'clock position in the top plate, right near the edge of the tape, so that cluster at the top is out.  But it looks like I have a ton of healthy samples on both plates to choose from. 

This is roughly a week... should I look to start transferring or wait a few more days?

And what do I do with these plates once the transfer has been completed? 

Bonus question: what's the best way to cut parafilm?  I bought a 4" wide roll because that's all I could find.... do I just cut in half as I go or is there a good way to split the whole roll? 


Edited by Ro0k (10/10/15 08:20 AM)


Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: When should I transfer plates? [Re: Ro0k]
    #22357947 - 10/10/15 08:02 AM (8 years, 3 months ago)

Transfer from the leading edge as far from contams as posible. Make your transfers as soon as you identify clean mycelium growth. Take as small a transfer as posible.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


Extras: Filter Print Post Top
OfflineRo0k
Stranger

Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
Re: When should I transfer plates? [Re: MudaFuka]
    #22357996 - 10/10/15 08:21 AM (8 years, 3 months ago)

So I assume transfer as soon as possible then, eh?  Like, today or tomorrow.  I imagine I've already waited a bit too long.


Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: When should I transfer plates? [Re: Ro0k]
    #22358021 - 10/10/15 08:28 AM (8 years, 3 months ago)

Yeah transfer ASAP.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Can you avoid G2G senescence by transferring flesh to Agar? sasquatchisreal 908 11 03/20/18 01:10 PM
by bodhisatta
* Re: mold in agar plate Anonymous 12,189 3 12/04/99 12:35 AM
by Anonymous
* agar transfers *DELETED* thisone 1,245 3 11/20/03 08:58 PM
by Mycomancer
* G2G transfers...an endless cycle? ADoobie 7,187 18 11/12/20 07:10 PM
by FredsGotSlacks
* Why different mycelium after transfer? archivist 1,216 7 02/09/09 07:47 PM
by Jitsu
* agar transfers buggers 4,514 12 11/03/07 10:44 AM
by orchidfanatic
* agar plate transfers..im not sure?? answers? wutang 1,963 7 11/21/07 12:16 PM
by total
* How to make agar 2 agar transfer? esp 6,655 7 01/08/04 11:08 AM
by YidakiMan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
280 topic views. 44 members, 346 guests and 52 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.007 seconds on 12 queries.