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OfflineLeeper
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Orange substance growing on mycelium...? Help!
    #22355970 - 10/09/15 07:17 PM (8 years, 3 months ago)

Had just inoculated several jars and kept them around 82 degrees F and after the mycelium started to form from the spores I noticed an orange substance on the cakes where the mycelium had started! It's hard to describe, but if you have ever bruised a cake and it turned blue, imagine that but orange! I threw them out because I figured it could be a contam but wanted to hear what others think. My first 5 cakes this didn't happen but every cake after that, even the ones about 12- 15 days after inoculation started getting orange. Anybody else having issues like this? I have tried to research it and found nothing like it, but if I'm a first timer and have seen it, I'm sure others have too! Any ideas?


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OfflinePinPornProducer
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Re: Orange substance growing on mycelium...? Help! [Re: Leeper]
    #22355977 - 10/09/15 07:18 PM (8 years, 3 months ago)

Dry ?


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OfflineLeeper
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Re: Orange substance growing on mycelium...? Help! [Re: PinPornProducer]
    #22356033 - 10/09/15 07:32 PM (8 years, 3 months ago)

Hygrometer says its 60-64% humidity in the container, but there is condensation in the jars which are in a "bucket in bucket" heating system. The bottom bucket has water and a heater and the top bucket holds my jars! They seem pretty moist...


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Don't just do drugs to do drugs, enjoy drugs! They are a great thing unless unwisely used!


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InvisibleBoomer The Great
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Re: Orange substance growing on mycelium...? Help! [Re: Leeper] * 1
    #22356429 - 10/09/15 08:55 PM (8 years, 3 months ago)

82 degrees F is too high of a temperature. You are going to be more prone to contamination. My jars are usually kept between 75-78 degrees F.


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InvisiblePastywhyteMDiscord
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Re: Orange substance growing on mycelium...? Help! [Re: Boomer The Great]
    #22356565 - 10/09/15 09:32 PM (8 years, 3 months ago)

Its either mold or its metabolites being formed from mycelium being stressed from the high temps.


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OfflineYaMoonSun
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Re: Orange substance growing on mycelium...? Help! [Re: Pastywhyte]
    #22356954 - 10/09/15 11:03 PM (8 years, 3 months ago)

Could moving them from warm to cool or any other sudden temperature change stress them out as well?


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InvisibleSupalemonhaze
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Re: Orange substance growing on mycelium...? Help! [Re: YaMoonSun]
    #22357712 - 10/10/15 05:49 AM (8 years, 3 months ago)

Or could be contam from the syringe I guess. I read that is pretty common.

Did you flame it?


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OfflineLeeper
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Re: Orange substance growing on mycelium...? Help! [Re: Supalemonhaze]
    #22357785 - 10/10/15 06:38 AM (8 years, 3 months ago)

I didn't flame the needle, but I alcohol swabbed it before every inoculation. I will bump down the temp on my next batch!  It's easier to keep it at that temp anyways! :smile: I have heard flaming and alcohol swabbing is just as good?! Should I do both, flame and then cool with alcohol swab?

Havnt been moving them from cool to warm and then back, it's pretty good at keeping a preferred temp setting. If I do move them it's to switch the upper jars with the lower jars to maintain a steady heat so my bottom jars don't get too hot and win the mycelium race before the others. They are out of their temp setting for maybe... A minute or two when switching, try to keep them as steady as possible!
How often should I air out my incubation chamber, if much at all?
Thanks for the good stuff guys, I'll change some stuff around and see if that doesn't help! But other posts with other ideas is always appreciated! :smile:


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OfflinePinPornProducer
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Re: Orange substance growing on mycelium...? Help! [Re: Leeper]
    #22357787 - 10/10/15 06:41 AM (8 years, 3 months ago)

Flame and never wipe the needle afterwards, that's like taking a shower and then drying off with an old ladies depends


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OfflineRogerRabbitM
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Re: Orange substance growing on mycelium...? Help! [Re: Supalemonhaze] * 2
    #22357793 - 10/10/15 06:44 AM (8 years, 3 months ago)

There are no orange contaminants that I've ever seen.  Excess temperature causes excess metabolites to form as you see below.  Depending on what the mycelium is munching, they can be orange or yellow, brown, etc.

You can also see how the mycelium has colonized the vermiculite.  Don't ever let idiots tell you that verm has no 'nutes'.  If there were just a few rhizos in the verm, they would be simply absorbing water like what happens when you fail to use foil and set a cake directly on the perlite.  When it's fuzzy mycelium, you can bet your butt it's feeding.

One other thing.  Always flame syringe needles until red hot, never wipe with alcohol.  Alcohol doesn't touch the contaminants on the inside of the needle, plus it's just a sanitizer. Alcohol doesn't sterilize.
RR



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InvisibleSupalemonhaze
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Re: Orange substance growing on mycelium...? Help! [Re: RogerRabbit]
    #22357807 - 10/10/15 06:50 AM (8 years, 3 months ago)

Cool RR!

Well last I heard verm is a volcanic material so its bound to have some minerals I'd think. Maybe thats what the mycelium is eating?


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OfflineYaMoonSun
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Re: Orange substance growing on mycelium...? Help! [Re: RogerRabbit]
    #22358149 - 10/10/15 09:14 AM (8 years, 3 months ago)

wb RR :cheers:
Does that imply that the vermiculite is going to be eventually broken down by the mycelium? I was purposely avoiding vermiculite for sclerotia production.


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InvisibleDaveyJones6911
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Re: Orange substance growing on mycelium...? Help! [Re: RogerRabbit]
    #22358240 - 10/10/15 09:44 AM (8 years, 3 months ago)

Quote:

RogerRabbit said:
There are no orange contaminants that I've ever seen.




doesn't lipstick mould occasionally do orange?


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Invisiblejbaby007
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Re: Orange substance growing on mycelium...? Help! [Re: RogerRabbit]
    #22358278 - 10/10/15 09:52 AM (8 years, 3 months ago)

Quote:

RogerRabbit said:
There are no orange contaminants that I've ever seen.  Excess temperature causes excess metabolites to form as you see below.  Depending on what the mycelium is munching, they can be orange or yellow, brown, etc.

You can also see how the mycelium has colonized the vermiculite.  Don't ever let idiots tell you that verm has no 'nutes'.  If there were just a few rhizos in the verm, they would be simply absorbing water like what happens when you fail to use foil and set a cake directly on the perlite.  When it's fuzzy mycelium, you can bet your butt it's feeding.

One other thing.  Always flame syringe needles until red hot, never wipe with alcohol.  Alcohol doesn't touch the contaminants on the inside of the needle, plus it's just a sanitizer. Alcohol doesn't sterilize.
RR





:prettyflyforawhiteguy: :incredible:  :youthemandawg:  :zomgteehee:  :kaneclap:


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InvisiblePastywhyteMDiscord
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Re: Orange substance growing on mycelium...? Help! [Re: DaveyJones6911]
    #22358301 - 10/10/15 09:57 AM (8 years, 3 months ago)

Quote:

DaveyJones6911 said:
Quote:

RogerRabbit said:
There are no orange contaminants that I've ever seen.




doesn't lipstick mould occasionally do orange?




No but neurospora does.



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OfflinePsilosopherr
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Re: Orange substance growing on mycelium...? Help! [Re: Pastywhyte]
    #22358550 - 10/10/15 10:47 AM (8 years, 3 months ago)

RR's officially back :awedance:


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OfflineLeeper
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Re: Orange substance growing on mycelium...? Help! [Re: RogerRabbit]
    #22362686 - 10/11/15 10:50 AM (8 years, 3 months ago)

Good stuff! I'll flame from now on, I was a bit worried about melting the place where the needle and syringe meet, apparently that shouldn't be an issue then.  No more alcohol for me, stinks anyways! Do you flame after every inoculation, or every separate jar?


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Don't just do drugs to do drugs, enjoy drugs! They are a great thing unless unwisely used!


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OfflineThatShroomer7
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Re: Orange substance growing on mycelium...? Help! [Re: Leeper]
    #22362738 - 10/11/15 11:03 AM (8 years, 3 months ago)

Flame between each injection to be safe


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OfflinePsilosopherr
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Re: Orange substance growing on mycelium...? Help! [Re: ThatShroomer7]
    #22362811 - 10/11/15 11:18 AM (8 years, 3 months ago)

yeah I'm always worried about melting that part of the syringe myself. Hasn't happened yet though. I've only seen it blacken.

How often I flame is based on whether or not the needle has touched something. More frequent flaming is safer obviously. Even if the needle touches nothing I'll still flame every 3 jars or so but thats me.


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OfflineLeeper
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Re: Orange substance growing on mycelium...? Help! [Re: Psilosopherr]
    #22364483 - 10/11/15 05:25 PM (8 years, 3 months ago)

Do u have issues with spitting spores/water outside of the syringe when reheating? Sucking the water with the spores back into the syringe seems bad if it's contaminated, then the whole syringe could be contaminated. And squirting the liquid out with the spores and reheating the needle seems to be a waste! Best method you have found?


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Don't just do drugs to do drugs, enjoy drugs! They are a great thing unless unwisely used!


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