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OfflineDinkinFlicka84
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Is this considered too much MS inoculant? I've heard 1-2cc, seems too much.
    #22353129 - 10/09/15 03:21 AM (8 years, 3 months ago)

The fact that I can see the liquid running down the side of the jar, reminds me of when I had bad spawn and the seeds had those water rings around them. I know this isn't the same thing, but I just wanted to get some pro advice on if this looks like too much. I only inoculated in one spot, so I didn't squirt the MS around the jar to hit multiple inoculant spots.

Should I have dropped MS in multiple sides of the jar?
Also, I've decided not to shake the jar after I knock up with MS. I would think most people who do MS don't shake immediately.

I just read that you want to watch for contamination, which is easier if all of your MS is in one spot, not shaken all over.
-Thanks, Chronicr



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InvisibleSupalemonhaze
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: DinkinFlicka84]
    #22353141 - 10/09/15 03:33 AM (8 years, 3 months ago)

1-2 cc is pretty much what any tek tells you to use so I think you are good to go. I have never used MS yet because I started with LC and agar so I don't really have experience with it.

I am however making an experiment with LC. I was inoculating some pf jars with a clone LC and when I got to the last jar I had like 4cc left. So instead of squirting it back into my LC jar I put all of it in my last jar for 2 reasons. A) I wanted to see if it will colonise faster and more importantly, B) I did not want to risk putting any contam back in my LC jar if I did anything wrong sterile technique wise.


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OfflineDinkinFlicka84
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: Supalemonhaze]
    #22353383 - 10/09/15 06:54 AM (8 years, 3 months ago)

Quote:

Supalemonhaze said:
1-2 cc is pretty much what any tek tells you to use so I think you are good to go. I have never used MS yet because I started with LC and agar so I don't really have experience with it.

I am however making an experiment with LC. I was inoculating some pf jars with a clone LC and when I got to the last jar I had like 4cc left. So instead of squirting it back into my LC jar I put all of it in my last jar for 2 reasons. A) I wanted to see if it will colonise faster and more importantly, B) I did not want to risk putting any contam back in my LC jar if I did anything wrong sterile technique wise.




It's not often I get to share my own personal experience that I feel confident enough to give advice, but I've done what you just mentioned and there's always a possibility it will work out just fine. However, in this case using 4 cc's has a decent chance of causing bacteria, which will weaken your bulk

I too, had the thought that putting more inoculant would yield faster colonization. What I recall was off-setting the water content by A LOT. Your seeds should be fairly dry and rattle around in the jar, but when you add 4 cc's to the jar, you're promoting contaminants.

I only express this to you for you doing your LC's later on. I did LC's as well for a good 8 months and I had TERRIBLE luck with my yields. They would normally contam during or even before the 1st flush, which was always a very small yield due to the bacteria the extra water from my LC's was causing. Standing water es no bueno, which took me the better part of a whole year to figure out.

Obviously you're not having these kinds of problems, but since you're experimenting with using a lot of inoculant, it really depends on how dry your grain is when you loaded your jars. You should post back and let us know what happens with your 4cc PF Jar.


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InvisibleSupalemonhaze
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: DinkinFlicka84]
    #22353472 - 10/09/15 07:28 AM (8 years, 3 months ago)

Quote:

DinkinFlicka84 said:
Quote:

Supalemonhaze said:
1-2 cc is pretty much what any tek tells you to use so I think you are good to go. I have never used MS yet because I started with LC and agar so I don't really have experience with it.

I am however making an experiment with LC. I was inoculating some pf jars with a clone LC and when I got to the last jar I had like 4cc left. So instead of squirting it back into my LC jar I put all of it in my last jar for 2 reasons. A) I wanted to see if it will colonise faster and more importantly, B) I did not want to risk putting any contam back in my LC jar if I did anything wrong sterile technique wise.




It's not often I get to share my own personal experience that I feel confident enough to give advice, but I've done what you just mentioned and there's always a possibility it will work out just fine. However, in this case using 4 cc's has a decent chance of causing bacteria, which will weaken your bulk

I too, had the thought that putting more inoculant would yield faster colonization. What I recall was off-setting the water content by A LOT. Your seeds should be fairly dry and rattle around in the jar, but when you add 4 cc's to the jar, you're promoting contaminants.

I only express this to you for you doing your LC's later on. I did LC's as well for a good 8 months and I had TERRIBLE luck with my yields. They would normally contam during or even before the 1st flush, which was always a very small yield due to the bacteria the extra water from my LC's was causing. Standing water es no bueno, which took me the better part of a whole year to figure out.

Obviously you're not having these kinds of problems, but since you're experimenting with using a lot of inoculant, it really depends on how dry your grain is when you loaded your jars. You should post back and let us know what happens with your 4cc PF Jar.




This is actually my first try using LC so I dont have any experience with yields yet. But yes I will let you guys know how all of my current projects go. Upsetting the water content actually makes a lotta sense so I hope all will be well.


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Invisiblewowimflabbergasted
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: Supalemonhaze]
    #22353517 - 10/09/15 07:42 AM (8 years, 3 months ago)

Nah man 4-5cc isn't going to do anything to the water content. I use, at least, 5-10cc of GLC/LI every time I knock up a grain jar. I've used 100cc and still got fruits from it lol.

You don't want too much MS because they are just spores trying to germinate and for that they (usually) need a partner. It only takes 2 spores so the more competing spores and genetics you put in that jar the more diverse they they will be making it sometimes harder to find good traits within all the shitty ones. There are millions and millions of spores in your syringe...don't worry about that.


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InvisibleMudaFuka
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: DinkinFlicka84]
    #22353658 - 10/09/15 08:37 AM (8 years, 3 months ago)

1/2 a cc is more than enough 5o inoculate a grain jar but using more won't really hurt. It just means your genetics will be more varied and you wasted a lot of spores.  Not the end of the world though. You are right don't shake after MS inoculation.


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OfflineDinkinFlicka84
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: MudaFuka]
    #22354249 - 10/09/15 11:13 AM (8 years, 3 months ago)

Quote:

MudaFuka said:
1/2 a cc is more than enough 5o inoculate a grain jar but using more won't really hurt. It just means your genetics will be more varied and you wasted a lot of spores.  Not the end of the world though. You are right don't shake after MS inoculation.




As far as water content, maybe it's just a persona experience, but I used to inoculate with 1.5-2cc's and get bacteria every single time, but then again I didn't dry my grains out like I do now. I suppose if your grains are super dry on the outside, then 4cc's wouldn't overload the jar with water. Especially if you shook the jar a few times to redistribute the water.


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Invisiblewowimflabbergasted
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: DinkinFlicka84]
    #22354258 - 10/09/15 11:16 AM (8 years, 3 months ago)

:lol:


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InvisibleSupalemonhaze
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: wowimflabbergasted]
    #22355112 - 10/09/15 03:54 PM (8 years, 3 months ago)

Whoa thats a lotta water xD. That's spelt right. Im using a millet/spelt mix myself


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InvisibleMudaFuka
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: Supalemonhaze]
    #22355127 - 10/09/15 03:58 PM (8 years, 3 months ago)

That's fucked up. The jar us full of water but the grains don't look fully hydrated.


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Invisiblewowimflabbergasted
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: MudaFuka]
    #22355624 - 10/09/15 06:02 PM (8 years, 3 months ago)

I got fruits from it :shrug: Check it!

Before after shake





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InvisibleMudaFuka
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: wowimflabbergasted]
    #22355694 - 10/09/15 06:24 PM (8 years, 3 months ago)

Oats will soak it up. If that was WBS it might not work out that way.


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Online99.99
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: MudaFuka]
    #22355847 - 10/09/15 06:53 PM (8 years, 3 months ago)

I've used as little as one drop and as much as a whole syringe ... They both worked fine on grain


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Invisiblewowimflabbergasted
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Re: Is this considered too much MS inoculant? I've heard 1-2cc, seems too much. [Re: MudaFuka]
    #22355876 - 10/09/15 07:00 PM (8 years, 3 months ago)

Quote:

MudaFuka said:
Oats will soak it up. If that was WBS it might not work out that way.



Yeah good point.


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