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OfflineGoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
Incubation Methods
    #22351888 - 10/08/15 08:36 PM (8 years, 3 months ago)

Hello, I have seen many different methods people use for mycelium growth; complete darkness, diffuse lighting, high temperatures, I am confused on what the best way yall have seen is to grow mycelium the fastest/healthiest way.  Any experiences or successful methods would be greatly appreciated.  I am using BRF, Vermiculite and water jars.


--------------------
Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money.  But hell life's right in front of me, nothing to look forward to.  Best of luck to all.


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OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Incubation Methods [Re: GoldenGrowBoy]
    #22351907 - 10/08/15 08:40 PM (8 years, 3 months ago)

Most will say room temp indirect light. I put mine on a shelf in my bedroom. That's it. Higher temps speed colonization but give a greater chance of contamination growing first. And then there's circadian rhythm.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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InvisibleKalistis
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Registered: 09/06/15
Posts: 2,265
Re: Incubation Methods [Re: PabloSumgie]
    #22351940 - 10/08/15 08:46 PM (8 years, 3 months ago)

... I am not sure how I feel about the statement "higher temps speeds up colonization." I think you should clarify what temps you are talking about.

I keep my home at a constant 74 degrees and it seems to be the perfect temp for colonization. Jars and tubs produce a fair amount of heat on their own that many don't account for when setting temps. The one day I didn't turn my air conditioner and my home went over 80 degrees, my WBS jars got metabolites and kinda stalled for 24 hours until they had a chance to recover.


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InvisibleMudaFuka
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Incubation Methods [Re: Kalistis] * 1
    #22352186 - 10/08/15 09:39 PM (8 years, 3 months ago)

I never let my Temps get higher than 76 during colonisation. I don't give light much thought until fruiting. It doesn't make much difference during colonisation IME.


--------------------
AMU
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Liquid Inoculant Tek                                   
                     


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OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Incubation Methods [Re: MudaFuka]
    #22352690 - 10/09/15 12:01 AM (8 years, 3 months ago)

My room temps are 68-72f. Some people believe 86f is the best temp for mycelium growth but those temperatures are also very beneficial for most if not all contaminants. Mycelium will die with temps over 100f(not sure exact). But it will also grow at temps into the high 50s, while most contams will struggle at temps that low. It is true that the myc growing causes heat. So too high of temps and you stall it. Low temps will make contams struggle, make it so they don't overheat, it will just take a few days longer. It's usually negligible IME. There are alot of opinions on this subject.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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OfflineCraterBait
the sexiest
Registered: 10/08/15
Posts: 240
Last seen: 2 years, 10 months
Re: Incubation Methods [Re: PabloSumgie]
    #22352976 - 10/09/15 01:25 AM (8 years, 3 months ago)

For incubation I use the simple 2-tote, water, aquarium heater method. My temp is at a stable 27C. For lighting I use a 450nm LED bulb inside the tub. I haven't had any issues yet, and I plan on trying this for the first time with Pan Cyanescens on my next grow. Works fine for cubes.


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InvisibleMudaFuka
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Incubation Methods [Re: CraterBait] * 1
    #22353428 - 10/09/15 07:10 AM (8 years, 3 months ago)

Growth actually slows down in the 80s. I can't remember the exact temp but I think it's around 84 degrees that cube mycelium growth slows. The temp inside the jars is a few degrees warmer than the ambient temp. So anything over 80 is not only raising the risk of contams but slowing down there myc. I really prefer much lower temps these days. I let my stuff colonise in the low to mid 60s.it's slightly slower but in the end I get thicker growth and fewer contams.


--------------------
AMU
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OfflineDrCrumbs
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Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
Re: Incubation Methods [Re: MudaFuka]
    #22353583 - 10/09/15 08:07 AM (8 years, 3 months ago)

When I started, I would use the water heater incubators and keep them around 82F degrees. They did very well. As time went on I ended up trying all sorts of different temperatures. They all worked well, not a huge difference.

There were study's done showing low 80F's work the best.
I dunno if these were done on an isolate or from MS.

I prefer to keep my house around 75F, and they do very well at this temperature.


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OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Incubation Methods [Re: DrCrumbs]
    #22353628 - 10/09/15 08:25 AM (8 years, 3 months ago)

Roger Rabbits videos say 81f. Growing magic mushroom made easy states 70-90f with 86f being preferred. Mycelium dies at 106f. Growth doesn't slow down untill the 90s.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: Incubation Methods [Re: DrCrumbs] * 1
    #22353646 - 10/09/15 08:32 AM (8 years, 3 months ago)

They slow down way before 90 and 86 is way above optimal. Even 81 is higher than I would go.  It's really not worth giving that much thought though.  Just stick them on a shelf and let them do there thing.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Incubation Methods [Re: MudaFuka]
    #22353992 - 10/09/15 10:07 AM (8 years, 3 months ago)

Spawn Run:
Relative Humidity: 90%.
Substrate Temperature: 84 - 86°F / 28.8 - 30°C. Thermal death limits have been established at 106°F / 41.1°C.
Duration: 10-14 days.
CO2: 5000 - 10,000 ppm.
Fresh Air Exchanges: 0 per hour.
From Paul stamets.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: Incubation Methods [Re: PabloSumgie]
    #22354014 - 10/09/15 10:12 AM (8 years, 3 months ago)

How long ago did he say that? I can saw without a doubt from personal experience. Jars colonise at there fastest in the high 70s.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflineDrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
Re: Incubation Methods [Re: MudaFuka]
    #22354179 - 10/09/15 10:55 AM (8 years, 3 months ago)

I dont remember but werent these experiments done with agar plates?
(Its been a long time since I read them).

This could account for the differences since your using a substrate. Maybe its just me but I've noticed that large grain jars give off more heat than pint brf jars.

As stated before the myc gives off heat and the inside temp of the jar will be greater. A plate doesnt offer the same environment.


Edited by DrCrumbs (10/09/15 10:57 AM)


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OfflineGoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
Re: Incubation Methods [Re: DrCrumbs]
    #22354204 - 10/09/15 11:04 AM (8 years, 3 months ago)

Thanks a lot my house runs at around 68 so I will just stick them on the shelf in an open top container for diffuse natural light I have a 6500K with 550/1400/2150 lumens of output as options, I will be using this for fruiting should I shine it indirectly on the mycelium for short periods a day during the spawn run?  Thanks again for all the responses super helpful to get so much first hand information.


--------------------
Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money.  But hell life's right in front of me, nothing to look forward to.  Best of luck to all.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Incubation Methods [Re: PabloSumgie]
    #22354379 - 10/09/15 11:52 AM (8 years, 3 months ago)

Quote:

PabloSumgie said:
Spawn Run:
Relative Humidity: 90%.
Substrate Temperature: 84 - 86°F / 28.8 - 30°C. Thermal death limits have been established at 106°F / 41.1°C.
Duration: 10-14 days.
CO2: 5000 - 10,000 ppm.
Fresh Air Exchanges: 0 per hour.
From Paul stamets.



first off paul was wrong then and is wrong now, in fact those numbers are not even his he simply ran with somebody' elses numbers from a notebook without testing it himself


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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Re: Incubation Methods [Re: PabloSumgie]
    #22354909 - 10/09/15 03:00 PM (8 years, 3 months ago)

Quote:

PabloSumgie said:
Substrate Temperature: 84 - 86°F / 28.8 - 30°C.



This is substrate temp, not incubation temp. if you bother to read the rest of the book you will see he specifically warns about the temp rise of substrates.

Many like to misquote him for some reason and say he recommended to incubate at 86F, he never did. Some are ignorant, some appear to have an issue with him.


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OfflineGoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
Re: Incubation Methods [Re: blackout]
    #22354920 - 10/09/15 03:03 PM (8 years, 3 months ago)

So Paul Stamets was cheat and liar.  But should I use diffuse CFL lighting during "incubation" (jars sitting on my shelf in an open top tub)


--------------------
Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money.  But hell life's right in front of me, nothing to look forward to.  Best of luck to all.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
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Re: Incubation Methods [Re: blackout]
    #22354922 - 10/09/15 03:03 PM (8 years, 3 months ago)

who could have a problem with paul:lol: RR i can easily see but pauls so nice


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleMudaFuka
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Incubation Methods [Re: cronicr]
    #22355058 - 10/09/15 03:42 PM (8 years, 3 months ago)

You can give them some indecent light if you want but it's not needed. Lately moste of my jars have been colonising in the back of a closet that only gets light for a few minutes hear and there.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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OfflineDrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
Re: Incubation Methods [Re: MudaFuka]
    #22359479 - 10/10/15 02:33 PM (8 years, 3 months ago)

I only make an effort to add light once they are placed into fruiting conditions. I never noticed that light even mattered that much when fruiting brf cakes. When using bulk substrates it defiantly makes a difference for me.

I do know that feeling that you just trying to make everything is optimal as possible. Its good that your trying to make everything optimal. Just dont worry to much. BRF cakes do pretty well, with just about anything you do to them.

The thing with cubes is they just love to consume and then fruit once they have eaten everything. This is partly why you see so much contradicting info.

Chances are you will do well. Sterile technique and correct moisture content (you dont want them swampy in the beginning) are what will help you succeed.


Edited by DrCrumbs (10/10/15 02:35 PM)


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