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American
Fuck yeah!

Registered: 09/25/15
Posts: 15
Last seen: 8 years, 3 months
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Laminar Flow Hood set up
#22351586 - 10/08/15 07:16 PM (8 years, 3 months ago) |
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I have a Series I laminar flow hood from Fungi Perfecti.
I love it, sooooo much better than my SAB!
I have a question, though:
The flow hood is encased in a 1.5 inch wooden frame. Because of that, the flow itself does NOT extend to the table surface.
How should I use the flow hood to maximize my sterile work area? Should I build a small base in front of the flow hood, 1.5 inches tall, so that the surface and tha laminar flow are level? Or is the 1.5 inches between the bottom of the filter and the table EFFECTIVELY sterile? It is, after all, benefitting from 12 inches of sterile flow above it.
Only answers from users of laminar flow, please. No Conjecture or educated guessing. Thanks!
Edited by American (10/08/15 07:20 PM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Laminar Flow Hood set up [Re: American]
#22351627 - 10/08/15 07:25 PM (8 years, 3 months ago) |
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i have seen people build small platforms, but u don't need to. i pour my plates right on the table, the first two are not technically in front of the filter. i don't know if mine is 1.5 inches at the bottom, but it shouldn't matter. i think the series 1 has a strong blower for filter size, so u should be fine.

wipe the surface with lysol or iso before pouring.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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American
Fuck yeah!

Registered: 09/25/15
Posts: 15
Last seen: 8 years, 3 months
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Awesome, thanks for the quick response, BlindingLeaf!
I was wondering, I use quart jars. Could I raise the FLOW HOOD itself by an inch or two, to effectively give me a ~14 inch tall hood? I Need sterile flow in the 6-14 inch level MORE than I need it along the table bottom itself. Grain to train transfer is tough when I worry about that top envelope being not-so-sterile.
I tested the flow with smoke from incense, and 10-12 inches from the table top was the weakest part of the flow. What do you think? Has anyone tried this?
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99.99
Stranger


Registered: 12/22/14
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Re: Laminar Flow Hood set up [Re: American]
#22351679 - 10/08/15 07:41 PM (8 years, 3 months ago) |
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I needed more room so I built this one day

The hood just sits on top so nothing is damaged with the original I'd like to ad a UV light but I don't know if it would help
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Laminar Flow Hood set up [Re: 99.99]
#22351712 - 10/08/15 07:48 PM (8 years, 3 months ago) |
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sure, u can kinda do whatever u want if u understand how to work in front of the hood. i just use mine as is for jars or bags or agar. with jars, i don't have an issue. with bags, i have to tilt them on the side to g2g. seems like that would be a more appropriate time to alter the height of the hood. with jars, i don't see a need to, but u can try it if u want.
thats pretty cool 99! a UV light can help. i don't use one, but i run my hood frequently enough I'm not worried about anything growing back in the box.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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American
Fuck yeah!

Registered: 09/25/15
Posts: 15
Last seen: 8 years, 3 months
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Thanks for the responses, guys!
99, that's a pretty neato macguyver kind of set up your made. But that makes it a VERTICAL laminar flow, right? And in that case you could have just spent $150 on a huge HEPA filter and gotten any old squirrel cage fan. The fact that the flow is laminar is kinda superfluous.
That said, it does seem pretty cool, especially to have that kind of space to do clean work in. What is your contamination rate? At the end of the day, that's all that matters, right!
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99.99
Stranger


Registered: 12/22/14
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Re: Laminar Flow Hood set up [Re: American]
#22352679 - 10/08/15 11:59 PM (8 years, 3 months ago) |
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Quote:
American said: Thanks for the responses, guys!
99, that's a pretty neato macguyver kind of set up your made. But that makes it a VERTICAL laminar flow, right? And in that case you could have just spent $150 on a huge HEPA filter and gotten any old squirrel cage fan. The fact that the flow is laminar is kinda superfluous.
That said, it does seem pretty cool, especially to have that kind of space to do clean work in. What is your contamination rate? At the end of the day, that's all that matters, right!
150 for a filter... What ??? I was going to buy this till I founf my heppa http://www.ebay.com/ulk/itm/301747108091 NEW ASTROCEL I 1000CFM CLEAN ROOM HEPA FILTER 24X24X12"
No idea that vertical or horizontal made the difference I just knew I wanted more space and since the area to put you hands into was the same size as the filter I think the air flow is the same as from just the heppa The only problem I have a problem is with bacteria and I'm going to buy a UV light for that this weekend I bought the hepa off eBay for 200 so it's all good
Edited by 99.99 (10/09/15 12:17 AM)
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Re: Laminar Flow Hood set up [Re: 99.99]
#22352856 - 10/09/15 12:42 AM (8 years, 3 months ago) |
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I use one of these baking racks for my agar work to keep my plates up higher in front of the filter. I wipe it down with ISO between uses, same with the table. I set everything else on the table
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Re: Laminar Flow Hood set up [Re: 99.99]
#22352885 - 10/09/15 12:49 AM (8 years, 3 months ago) |
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The 12" deep filters are usually a lot cheaper online than the 5.8-6" deep ones. Not sure why
Thry work fine theyre just really bulky, can be heavy. You need at least a 12" plenum behind the filter too so that adds a lot to the bulk, its why most people prefer the 6" ones , less weight , 6 inch plenum means smaller hood
--------------------
 Amateur Mycologists United AMU Q&A
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American
Fuck yeah!

Registered: 09/25/15
Posts: 15
Last seen: 8 years, 3 months
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Re: Laminar Flow Hood set up [Re: mushpunx]
#22357158 - 10/10/15 12:05 AM (8 years, 3 months ago) |
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99, there is a HUGE difference between having sterile air and a laminar flow. There are dozens of threads about it, especially with RR weighing in pro-laminar.
But if having a vertical HEPA filter works for you, then that's all that really matters.
Mushpunx, it looks like you made your hood, what did it run you?
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RJ Tubs 202



Registered: 09/20/08
Posts: 6,016
Loc: USA
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Re: Laminar Flow Hood set up [Re: American]
#22357201 - 10/10/15 12:21 AM (8 years, 3 months ago) |
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Quote:
American said:
Should I build a small base in front of the flow hood, 1.5 inches tall, so that the surface and the laminar flow are level? Or is the 1.5 inches between the bottom of the filter and the table EFFECTIVELY sterile? It is, after all, benefiting from 12 inches of sterile flow above it.
I used this exact hood for many years. I used a 3/4 inch thick vinyl covered wood shelf (used for a bookcase) in front of the hood for my working area (which allowed me to wipe it down - contact paper on wood also works) ....but I think you could get away with doing nothing.
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