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Offlineucsdmolpath
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Registered: 12/26/03
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Last seen: 12 years, 2 months
Plug use and various antibiotics?
    #2235073 - 01/11/04 06:25 PM (12 years, 10 months ago)

hi everyone,
i had two questions maybe y'all have had some experience with...

1) has anyone tried to innoculate jars of sterilized media with commercially available plugs that are not sterile (just plop a plug into a jar), and successfully colonized without major contamination problems?

2) if the answer to my first question is no, in which case i would need to purify the spawn by growing on agar....i was wondering if single or combinatorial doses of either ampicillin, kanamycin and or gentamycin would provide enough antibiotic protection for viable growth...or too much antibiotic protection?  basically...which antibiotic alone or in combination is the best?

thanks for your advice in advance!
molpath :smile:


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InvisibleYidakiMan
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Re: Plug use and various antibiotics? [Re: ucsdmolpath]
    #2235186 - 01/11/04 07:29 PM (12 years, 10 months ago)

Use a knife to scrape tiny fragments of mycelium off of the dowel onto peroxidated agar plates. Do a bunch of plates to ensure success. If you get contams transfer clean mycelium away from contam.

Don't bother with antibiotics...


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Offlinem0rb
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Registered: 05/07/03
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Re: Plug use and various antibiotics? [Re: ucsdmolpath]
    #2235235 - 01/11/04 08:13 PM (12 years, 10 months ago)

I agree with YidakiMan, use peroxidated agar plates, and you should not need any antibiotics. You will most definitely need a flow hood or glovebox to do this with decent degrees of sucess.


-m0b-


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"The business of America is business," - Calvin Coolidge


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Offlineragadinks
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Re: Plug use and various antibiotics? [Re: ucsdmolpath]
    #2236465 - 01/12/04 06:03 AM (12 years, 10 months ago)

I have done that even without peroxidated agar/antibiotics. Have just put a dowel on a plate with wet cardboard. When the myc grew I isolated it away from the contams onto agar plates. But I have to admit that this might work only for woodloving species. H202 is probably the better/easier way.

In this thread there are some nice pics of spores beeing grown on cardboard and peroxidatet agar ( second page ).


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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