Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood

Jump to first unread post. Pages: 1
Offlineucsdmolpath
Stranger
Registered: 12/26/03
Posts: 19
Last seen: 12 years, 3 months
Plug use and various antibiotics?
    #2235073 - 01/11/04 06:25 PM (13 years, 9 days ago)

hi everyone,
i had two questions maybe y'all have had some experience with...

1) has anyone tried to innoculate jars of sterilized media with commercially available plugs that are not sterile (just plop a plug into a jar), and successfully colonized without major contamination problems?

2) if the answer to my first question is no, in which case i would need to purify the spawn by growing on agar....i was wondering if single or combinatorial doses of either ampicillin, kanamycin and or gentamycin would provide enough antibiotic protection for viable growth...or too much antibiotic protection?  basically...which antibiotic alone or in combination is the best?

thanks for your advice in advance!
molpath :smile:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleYidakiMan
Stranger
Male User Gallery
Registered: 09/29/02
Posts: 2,023
Re: Plug use and various antibiotics? [Re: ucsdmolpath]
    #2235186 - 01/11/04 07:29 PM (13 years, 9 days ago)

Use a knife to scrape tiny fragments of mycelium off of the dowel onto peroxidated agar plates. Do a bunch of plates to ensure success. If you get contams transfer clean mycelium away from contam.

Don't bother with antibiotics...


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinem0rb
mushroom mobster

Registered: 05/07/03
Posts: 184
Loc: New England
Last seen: 12 years, 6 months
Re: Plug use and various antibiotics? [Re: ucsdmolpath]
    #2235235 - 01/11/04 08:13 PM (13 years, 9 days ago)

I agree with YidakiMan, use peroxidated agar plates, and you should not need any antibiotics. You will most definitely need a flow hood or glovebox to do this with decent degrees of sucess.


-m0b-


--------------------
"The business of America is business," - Calvin Coolidge


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 2 years, 9 months
Re: Plug use and various antibiotics? [Re: ucsdmolpath]
    #2236465 - 01/12/04 06:03 AM (13 years, 8 days ago)

I have done that even without peroxidated agar/antibiotics. Have just put a dowel on a plate with wet cardboard. When the myc grew I isolated it away from the contams onto agar plates. But I have to admit that this might work only for woodloving species. H202 is probably the better/easier way.

In this thread there are some nice pics of spores beeing grown on cardboard and peroxidatet agar ( second page ).


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* % Gentamycin in Spore Syringe? Joshua 1,194 11 04/25/03 12:32 AM
by Odin 13
* natural antibiotic? x7x_x7x 1,826 12 10/03/08 10:56 PM
by CheeWiz
* Best Antibiotic for Spore Syringes?
( 1 2 all )
Joshua 2,857 23 06/18/03 04:25 AM
by Joshua
* antibiotic syringe? felixhigh 1,122 4 05/14/02 10:28 PM
by felixhigh
* Antibiotic concentrations in Agar cheesenoonions 1,993 9 12/30/02 01:40 AM
by Zen Peddler
* Strength of Kanamycin? sci33 1,291 9 07/02/04 05:53 PM
by tubervegan
* amoxicillin clavulanate - or other antibiotics altan 1,129 10 01/07/04 07:04 AM
by G a n j a
* aquatic antibiotics YidakiMan 1,063 8 05/03/04 10:43 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
733 topic views. 1 members, 3 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.005 seconds on 14 queries.