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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22375737 - 10/13/15 08:58 PM (8 years, 3 months ago) |
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Quote:
GreenHornThumb said:
Quote:
Psilosoulful said:
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GreenHornThumb said: Should I start prepping / trying agar and practice with some syringes ?


I'll agar research tomorrow to see how I want to do it.
Edited by Psilosoulful (10/13/15 11:28 PM)
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
#22377066 - 10/14/15 05:27 AM (8 years, 3 months ago) |
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I wonder how it would be if it was simmered/boiled with gypsum. And no that top right is not green just wierd angle/shadow of underside of kernel that was split in half I think.
Edited by GreenHornThumb (10/14/15 05:35 AM)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22377200 - 10/14/15 07:00 AM (8 years, 3 months ago) |
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Gypsum is mostly added during simmering/boiling to help decrease the amount of clumping between grains after the PC cycle.
If you were using WBS or rye berries, then they would have been a bit clumpier, because of their smaller size and higher surface area between individual grains.
Your popcorn grains look fine tho. 
Make sure to give it a good shake, if you haven't already, to redistribute that mycelium for faster growth!
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
#22377326 - 10/14/15 08:03 AM (8 years, 3 months ago) |
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Quote:
Psilosoulful said: Gypsum is mostly added during simmering/boiling to help decrease the amount of clumping between grains after the PC cycle.
If you were using WBS or rye berries, then they would have been a bit clumpier, because of their smaller size and higher surface area between individual grains.
Your popcorn grains look fine tho. 
Make sure to give it a good shake, if you haven't already, to redistribute that mycelium for faster growth!
I gave it a good shake at 25%. The myc was only engulfing the bottom half of jar. Now after shake it's taking over. I believe a good 3 or 4 more days and I'll give it the old 100% shake and let it sit for a few more.
Oh just for clumping? I thought it provided a bit of nutrients in the grain and substrate?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22377359 - 10/14/15 08:12 AM (8 years, 3 months ago) |
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Quote:
GreenHornThumb said:
Quote:
Psilosoulful said: Gypsum is mostly added during simmering/boiling to help decrease the amount of clumping between grains after the PC cycle.
If you were using WBS or rye berries, then they would have been a bit clumpier, because of their smaller size and higher surface area between individual grains.
Your popcorn grains look fine tho. 
Make sure to give it a good shake, if you haven't already, to redistribute that mycelium for faster growth!
I gave it a good shake at 25%. The myc was only engulfing the bottom half of jar. Now after shake it's taking over. I believe a good 3 or 4 more days and I'll give it the old 100% shake and let it sit for a few more.
Oh just for clumping? I thought it provided a bit of nutrients in the grain and substrate?
After the jar is 100%, then shake it, and spawn right away! No need to let it sit to recolonize.
Yes, it does also provide a bit of nutrients for the grains to absorb. Some people use it, others don't, it's not a deal breaker. Your grains will colonize either way.
But, it is most definitely needed for bulk subs. It provides nutrients for the growing mycelium as well as keeping the mix light and airy, in order for the mycelium to effectively colonize all parts of the substrate. A handful of gypsum is a good amount to put in with each small brick of coir that you'll be hydrating.
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
#22377519 - 10/14/15 09:05 AM (8 years, 3 months ago) |
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Quote:
Psilosoulful said:
Quote:
GreenHornThumb said:
Quote:
Psilosoulful said: Gypsum is mostly added during simmering/boiling to help decrease the amount of clumping between grains after the PC cycle.
If you were using WBS or rye berries, then they would have been a bit clumpier, because of their smaller size and higher surface area between individual grains.
Your popcorn grains look fine tho. 
Make sure to give it a good shake, if you haven't already, to redistribute that mycelium for faster growth!
I gave it a good shake at 25%. The myc was only engulfing the bottom half of jar. Now after shake it's taking over. I believe a good 3 or 4 more days and I'll give it the old 100% shake and let it sit for a few more.
Oh just for clumping? I thought it provided a bit of nutrients in the grain and substrate?
After the jar is 100%, then shake it, and spawn right away! No need to let it sit to recolonize.
Yes, it does also provide a bit of nutrients for the grains to absorb. Some people use it, others don't, it's not a deal breaker. Your grains will colonize either way.
But, it is most definitely needed for bulk subs. It provides nutrients for the growing mycelium as well as keeping the mix light and airy, in order for the mycelium to effectively colonize all parts of the substrate. A handful of gypsum is a good amount to put in with each small brick of coir that you'll be hydrating.
Ah just quickly searched I guess the sitting period after 100% colonization are for cakes?
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22377532 - 10/14/15 09:11 AM (8 years, 3 months ago) |
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Yup
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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How long can you store 50/50 mixes? Wife is getting the pint jars. Going to try and g2g the 3 jars evenly to 2 pint jars and use the left over 1/2 pints to fruit in a dub tub shoe box style.
Edited by GreenHornThumb (10/15/15 05:16 AM)
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22381979 - 10/15/15 05:17 AM (8 years, 3 months ago) |
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Updated OP time line

I believe they could be spawned on Saturday but my gypsum isn't here yet. I might go try find some drywall lying around and make a little bit of my own well see
Edited by GreenHornThumb (10/15/15 05:47 AM)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22382503 - 10/15/15 09:06 AM (8 years, 3 months ago) |
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It's slow growth for sure. It should've been fully colonized by now.
Loose polyfill causing excessive GE or a contam hiding in the grains could be some potential causes.
I'd give it another quick shake and see how fast it recolonizes. If it doesn't colonize completely after two days, then you'll have to open it up in a SAB and re-stuff that polyfill tightly, it's your only other option IMO.
And for next time, skip the popcorn and get a bag of whole oats. They're soo much cheaper, colonize a lot better, and are very easy to prep. I got a 50lb bag for 15$
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
#22382527 - 10/15/15 09:19 AM (8 years, 3 months ago) |
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Quote:
Psilosoulful said: It's slow growth for sure. It should've been fully colonized by now.
Loose polyfill causing excessive GE or a contam hiding in the grains could be some potential causes.
I'd give it another quick shake and see how fast it recolonizes. If it doesn't colonize completely after two days, then you'll have to open it up in a SAB and re-stuff that polyfill tightly, it's your only other option IMO.
And for next time, skip the popcorn and get a bag of whole oats. They're soo much cheaper, colonize a lot better, and are very easy to prep. I got a 50lb bag for 15$ 
Ill pack it tighter next time, I'll probably get some SHIP lids with filter disks soon.
Edited by GreenHornThumb (10/16/15 11:28 PM)
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22391370 - 10/16/15 11:32 PM (8 years, 3 months ago) |
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It's right on track with the 100% shake and it should be fully recovered tomorrow. I have my coir and verm ready to prep and a jar for a quick g2g transfer from one of the jars. It's cooling over night.
Ordered agar stuff and will be doing the No Pour Agar TeK
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb] 1
#22391388 - 10/16/15 11:36 PM (8 years, 3 months ago) |
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
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Last seen: 6 months, 17 days
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Quote:
wowimflabbergasted said: Check this out while you're at it. http://www.shroomery.org/forums/showflat.php/Number/19208976#19208976
I did it will be a mixture of the two.I like the glad minis for equipment but the prep time of the no pour. I guess I shoulda stated that. I got some glad minis , I also found this that seemed interesting http://www.shroomery.org/forums/showflat.php/Number/21907688
Edited by GreenHornThumb (10/16/15 11:50 PM)
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22391444 - 10/16/15 11:57 PM (8 years, 3 months ago) |
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Just some food for thought. You can PC your agar plates effectively for as low as 15-20 minutes, no need to sit around waiting for it to get to 45 minutes, save yourself some time. Only when you want to pour agar from a glass vessel, it'll need a longer PC time because there's more liquid. I haven't tried pouring plates yet I find Pasty's tek good enough for my needs.
And if you want to limit the amount of condensation on the inside of your glad mini's ( it's annoying when it pools up on the surface and you have to pour it out in your SAB) try reducing the pressure slowly at the end of the cycle, then remove from the heat source and let it cool completely.
I also found that when using half pint wide mouth glass jars ( the ones used for brf cakes) for my agar, there is virtually no condensation on the sides and there is also a bit more surface area for the mycelium to grow. You should try it out for your next round of agar!
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
#22393322 - 10/17/15 01:40 PM (8 years, 3 months ago) |
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Quote:
Psilosoulful said: Just some food for thought. You can PC your agar plates effectively for as low as 15-20 minutes, no need to sit around waiting for it to get to 45 minutes, save yourself some time. Only when you want to pour agar from a glass vessel, it'll need a longer PC time because there's more liquid. I haven't tried pouring plates yet I find Pasty's tek good enough for my needs.
And if you want to limit the amount of condensation on the inside of your glad mini's ( it's annoying when it pools up on the surface and you have to pour it out in your SAB) try reducing the pressure slowly at the end of the cycle, then remove from the heat source and let it cool completely.
I also found that when using half pint wide mouth glass jars ( the ones used for brf cakes) for my agar, there is virtually no condensation on the sides and there is also a bit more surface area for the mycelium to grow. You should try it out for your next round of agar! 
Sweet good to know . I just did a g2g transfer to a 1 pint jar of oats and a ms inoculation to a 1/2 pint oats. I did not know my pressure cooker needs a lower rack. Rack was too high and had to use some lids so the pint jar could fit. So I fit a 1/2 pint and 1 pint in there until my lower rack comes. I will update pics once I'm done prepping my substrate and spawn mix.
bump
Added to tub and cased
Edited by GreenHornThumb (10/17/15 04:16 PM)
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22399243 - 10/18/15 06:31 PM (8 years, 3 months ago) |
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My g2g jar is going good. I see some corn pieces attacking the oats. Also inoculated some brf jars because I really want to birth a cake.
Agar stuff comes tomorrow so that will be some fun to pass the time. I really want to go hunting. I know there are some nice ones around my area according to my location.
Updated op
Edited by GreenHornThumb (10/18/15 06:32 PM)
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Psilosoulful

Registered: 09/05/14
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Last seen: 1 year, 1 month
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
#22399274 - 10/18/15 06:35 PM (8 years, 3 months ago) |
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GreenHornThumb
Whisperer Of The Myc



Registered: 10/08/15
Posts: 367
Last seen: 6 months, 17 days
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
#22399333 - 10/18/15 06:45 PM (8 years, 3 months ago) |
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Quote:
Psilosoulful said:

I'm really interested in the g2g jar. Since I shook it there should be portions where the corn touched the oats and it should grow right ? Or does the myc actually just grow from the corn and attach to the oats ?
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