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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
    #22523326 - 11/14/15 04:21 PM (8 years, 2 months ago)

I inoculated 4 jars same syringe but I had maybe 3.5 soluton. I tried to give each enough evenly but with flame sterilization I think I evaporated some for the last jar and nothing was pushed in. It was a bit dark couldnt see how much he was left.

Now only 3 jars show normal growth while 1 shows nothing. Should I re-inoculate? Is there harm if there is or isn't any myc in a jar that has been sitting and re-inoculate again ? Since it's sterile I should be able to re-inoculate fine?


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Invisiblewowimflabbergasted
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22523331 - 11/14/15 04:23 PM (8 years, 2 months ago)

How long has it been


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: wowimflabbergasted]
    #22523337 - 11/14/15 04:25 PM (8 years, 2 months ago)

Quote:

wowimflabbergasted said:
How long has it been




I forgot to label but its thr average time its suppose to. But all 3 started showing growth together while 1 did not show anything. I was even expecting the top to see maybe if it was only a bit that was injected and maybe it stayed on top.


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Invisiblewowimflabbergasted
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22523346 - 11/14/15 04:28 PM (8 years, 2 months ago)

Eh I don't know man. If you think you did something wrong with it and it's showing signs that you did, I would knock it up again before the cakes dry out. OR wait a few more days then try it. It's up to you. Remember though, patience is key!


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: wowimflabbergasted]
    #22523404 - 11/14/15 04:42 PM (8 years, 2 months ago)

Quote:

wowimflabbergasted said:
Eh I don't know man. If you think you did something wrong with it and it's showing signs that you did, I would knock it up again before the cakes dry out. OR wait a few more days then try it. It's up to you. Remember though, patience is key!



Quote:

wowimflabbergasted said:
Eh I don't know man. If you think you did something wrong with it and it's showing signs that you did, I would knock it up again before the cakes dry out. OR wait a few more days then try it. It's up to you. Remember though, patience is key!




My b it was oats


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Invisiblewowimflabbergasted
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22523415 - 11/14/15 04:44 PM (8 years, 2 months ago)

Ah okay. Wait a few days.


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: wowimflabbergasted]
    #22523918 - 11/14/15 06:58 PM (8 years, 2 months ago)



Kinda new to this monotub. I tried to do it like my mini 4qt. Looks like it's colonizing. Nothing looks wierd or smells different. I'm used to the correct smell.  Seems few more days for the small patches to be colonized. Does it need more GE? I do see one exposed grain. I will apply a small casing layer maybe but I did so good with my 2liter with no casing I might leave it.


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OfflinePsilosoulful

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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22525057 - 11/14/15 09:54 PM (8 years, 2 months ago)

Did you drill holes all around the bottom of the tub, cuz it sure looks like it. That could be why the mycelium is stalling, there's wayy too much GE.


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Psilosoulful]
    #22526298 - 11/15/15 05:15 AM (8 years, 2 months ago)

Quote:

Psilosoulful said:
Did you drill holes all around the bottom of the tub, cuz it sure looks like it. That could be why the mycelium is stalling, there's wayy too much GE.





No I followed the doc t guide . Should I wrap it a bit tighter? I poked a few a holes because I thought it was needed?

https://www.shroomery.org/forums/showflat.php/Number/10713572/fpart/1/vc/1


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OfflineMachiavelliavore
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22526365 - 11/15/15 06:20 AM (8 years, 2 months ago)

Quote:

GreenHornThumb said:


Kinda new to this monotub. I tried to do it like my mini 4qt. Looks like it's colonizing. Nothing looks wierd or smells different. I'm used to the correct smell.  Seems few more days for the small patches to be colonized. Does it need more GE? I do see one exposed grain. I will apply a small casing layer maybe but I did so good with my 2liter with no casing I might leave it.




Why do you think somethings wrong?  It looks okay to me, maybe did a shitty job mixing the sub up?

I just stack my Doc_T tubs, then drop a plastic trash bag over the top of the stack to colonize them.  NP.  Case around 95% if you use a the setup I mentioned, it gets a bit of FAE from the hanging bag, and may start pinning if it's agro genetics.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (11/15/15 06:21 AM)


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Machiavelliavore]
    #22526482 - 11/15/15 07:29 AM (8 years, 2 months ago)

Quote:

Machiavelliavore said:
Quote:

GreenHornThumb said:


Kinda new to this monotub. I tried to do it like my mini 4qt. Looks like it's colonizing. Nothing looks wierd or smells different. I'm used to the correct smell.  Seems few more days for the small patches to be colonized. Does it need more GE? I do see one exposed grain. I will apply a small casing layer maybe but I did so good with my 2liter with no casing I might leave it.




Why do you think somethings wrong?  It looks okay to me, maybe did a shitty job mixing the sub up?

I just stack my Doc_T tubs, then drop a plastic trash bag over the top of the stack to colonize them.  NP.  Case around 95% if you use a the setup I mentioned, it gets a bit of FAE from the hanging bag, and may start pinning if it's agro genetics.



I tried mixing up evenly as possible, it is a lower spawn to sub ratio to test it out.


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OfflineMachiavelliavore
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22526501 - 11/15/15 07:39 AM (8 years, 2 months ago)

Top layer I assume?  My 1:3 and 1:4 have been colonizing in like 4-7 days.  How old is that?  You probably didn't have a level sub.

I like to mix my shit in a separate tote.  You really can't go at it appropriately having to not mess with the liner and get sub in the holes.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineGreenHornThumb
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Registered: 10/08/15
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Re: First Timer did lots of reading. Popcorn TeK. [Re: Machiavelliavore]
    #22526540 - 11/15/15 07:58 AM (8 years, 2 months ago)

Quote:

Machiavelliavore said:
Top layer I assume?  My 1:3 and 1:4 have been colonizing in like 4-7 days.  How old is that?  You probably didn't have a level sub.

I like to mix my shit in a separate tote.  You really can't go at it appropriately having to not mess with the liner and get sub in the holes.




Nah not top / casing layer yet. I might not since it's such a low spawn. I will check back again today if I see no changes not sure what to do. I know it's wrong to introduce fruiting conditions when not fully colonized.  I'll start mixing it in a separate tote. This is about a bit longer than 5 or so. If it was smaller substrate pretty sure it woulda been fully colonized.


update

Some seeds are colonizing,  I guess only a bit trickled on top and didnt make it down I'll try to see if it'll get to 30% drop top down . If not I might have to shake a little early.


Edited by GreenHornThumb (11/15/15 03:45 PM)


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22536507 - 11/17/15 09:01 AM (8 years, 2 months ago)

No cool updates yet things are going.

My first clone is actually recovering and I am starting to see white myc. One part looks like a piece of the tissue is melting ? I'll try take a picture but seems a bit wierd.

My 2 jars of apes show no signs of growth while other 4 jars of b+ are booming with myc.

My syringes did come from a reputable source. The Spore were a golden/bronze color really clumped together almost gelatinous like. So I shook as much as I could.

Not too sure if I didn't shake it enough or if it was frozen via shipping?  The liquid inside was fluid but the spores were together.

Checked on the syringes the other day and the gelatinous spores were all broken up ?


Starting to see a 3rd flush coming in my dub tub. And second flush on my 2liter.

Tempted to noc up more jars of apes to test the cause.


Edited by GreenHornThumb (11/17/15 09:56 AM)


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Invisiblewowimflabbergasted
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22536630 - 11/17/15 09:43 AM (8 years, 2 months ago)

I have 3 APE syringes from a vendor that are nothing but bacteria. I had a PE syringe that wouldn't germinate on anything at all. Sometimes it happens but its more frequent with PE varieties I've noticed.


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: wowimflabbergasted]
    #22536682 - 11/17/15 09:57 AM (8 years, 2 months ago)

Quote:

wowimflabbergasted said:
I have 3 APE syringes from a vendor that are nothing but bacteria. I had a PE syringe that wouldn't germinate on anything at all. Sometimes it happens but its more frequent with PE varieties I've noticed.




Hmm good to know, any way to test it other than trying make more jars ? I even put few drops on agar on the 13th I believe and I see nothing.


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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22537188 - 11/17/15 11:56 AM (8 years, 2 months ago)

Agar is the way. Sometimes it takes a bit longer for them to germ, if they do at all.


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: wowimflabbergasted]
    #22537513 - 11/17/15 01:02 PM (8 years, 2 months ago)

Quote:

wowimflabbergasted said:
Agar is the way. Sometimes it takes a bit longer for them to germ, if they do at all.




Gotcha I'll also inoc few more jars tonight, maybe I really didn't shake it correctly first time.

Really wanted to get apes going was excited upgrading from b+.



this!! It was exactly like mine . Super clumped up like jelly.

Quote:

Temporary Death said:
This is my first post, I have been a very long time Shroomery follower and only recently gotten into cultivation this year.
I am curious about an order that I placed through a spore sponsor,  i've been very happy with all of my previous purchases and this purchase included, but one of these 5 syringes (albino penis envy) I'm curious if this is for sure germinated spores within the solution, and/or if this breed is known to have white spores that I'm seeing?
If this is growing mycelium (created 9/15) when would it be recommended to use this syringe by at the latest?
Thanks everyone! I think this should be in the correct section.





And also I was hoping to save my first post to share my experience of reach complete death with the help from our fungal friends here, that will have to be saved for later tho! Thanks!





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Invisiblewowimflabbergasted
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Re: First Timer did lots of reading. Popcorn TeK. [Re: GreenHornThumb]
    #22537592 - 11/17/15 01:18 PM (8 years, 2 months ago)

That syringe looks fine. There's millions of spores in the area without the clump. :shrug:


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OfflineGreenHornThumb
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Re: First Timer did lots of reading. Popcorn TeK. [Re: wowimflabbergasted]
    #22537611 - 11/17/15 01:22 PM (8 years, 2 months ago)

Quote:

wowimflabbergasted said:
That syringe looks fine. There's millions of spores in the area without the clump. :shrug:




Hmm I'll isolate one jar with higher temp and see. I understand the risk of contam but if I see at least myc growth in one jar I know other jar will eventually grow. All were treated and tekd the same. This baffles me.


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