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OfflineMajora123
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Brf lipstick mold? Can you please help me identify
    #22349843 - 10/08/15 12:03 PM (8 years, 3 months ago)

Hello about a week and a half ago I made Brf cakes with mycelium agar wedges placed on top of them. I have no dry vermiculite later and am just using foil for the filter. Two holes on each of my four jars all are growing but 1 of the 4 looks different. Can anyone let me know if this is lipstick mold. One side of the jar is growing fast but the org side where it looks like the cake got too wet is growing slow. There is also a little discoloration on the side. The last pic is where there was a pink dot on the left side the mycelium is slowing covering is that normal for discoloration on bed cakes?


Edited by Majora123 (10/08/15 12:05 PM)


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OfflineMachiavelliavore
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Re: Brf lipstick mold? Can you please help me identify [Re: Majora123] * 1
    #22349884 - 10/08/15 12:13 PM (8 years, 3 months ago)

Looks bacterial.  That coloration looks like it is probably bacteria interacting with mycellium.

Two problems with what you're doing (at least:)
Tin foil for a filter is not a real filter by any reach, just hoping that no air current carries anything in there.  It might work, but seriously, go to the post office and get some free tyvek, or grab some polyfill.  There are plenty of borderline free options.

Inoculating a PF cake with an agar wedge is likely to stall anyway.  You're asking a single point inoculation to colonize an entire cup.  Can't see that completing in under a month even if it works.  If you want to use agar on PF cakes, check out Mudafuka's LI tek and Eatyualive's 9er tek to pf, which could be done with LI as easily as ground stem material.  All you need is an oster blender.

I assume you have a pressure cooker if you're doing agar work.  Just inoculate grains with agar.  Agar to PF will probably be ineffective without LI, or agar-->lc-->pf


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (10/08/15 12:22 PM)


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OfflineFreeWorldOrder
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Re: Brf lipstick mold? Can you please help me identify [Re: Majora123]
    #22349886 - 10/08/15 12:14 PM (8 years, 3 months ago)

:whathesaid:

Sorry, but those all looked contaminated...



--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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OfflineMajora123
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Re: Brf lipstick mold? Can you please help me identify [Re: Machiavelliavore] * 1
    #22349907 - 10/08/15 12:22 PM (8 years, 3 months ago)

Quote:

Machiavelliavore said:
Looks bacterial.  That coloration looks like it is probably bacteria interacting with mycellium.

Two problems with what you're doing (at least:)
Tin foil for a filter is not a real filter by any reach, just hoping that no air current carries anything in there.  It might work, but seriously, go to the post office and get some free tyvek, or grab some polyfill.  There are plenty of borderline free options.

Inoculating a PF cake with an agar wedge is likely to stall anyway.  You're asking a singe point inoculation to colonize an entire cup.  Can't see that completing in under a month even if it works.  If you want to use agar on PF cakes, check out Mudafuka's LI tek and Eatyualive's 9er tek to pf, which could be done with LI as easily as ground stem material.  All you need is an oster blender.

I assume you have a pressure cooker if you're doing agar work.  Just inoculate grains with agar.  Agar to PF will probably be ineffective without LI, or agar-->lc-->pf



Hello! These were just test jars I wanted to see if I got any growth. I used 4 wedges on each jar. I read on another forum that people grow shrooms just using a glass cut with foil over with success. These grow in the wild on top of shit so I don't know why we have to be so sanitary I guess it's because so many houses have the common green mold growing inside the homes. This is the only jar that looks discolored I just decided to take the foil off and smell inside and it's smells earthy. Should I just keep this one separate and wait? And if the jar covers these spots does that mean the mycelium fought it off and covered the contams. Will there still be contams under or will it eventually eat through it and reemerge?


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OfflineMajora123
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Re: Brf lipstick mold? Can you please help me identify [Re: FreeWorldOrder]
    #22349909 - 10/08/15 12:23 PM (8 years, 3 months ago)

Quote:

FreeWorldOrder said:
:whathesaid:

Sorry, but those all looked contaminated...





The pics are just of one jar. The rest don't look wet like that


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OfflineMachiavelliavore
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Re: Brf lipstick mold? Can you please help me identify [Re: Majora123]
    #22349938 - 10/08/15 12:33 PM (8 years, 3 months ago)

Those grows do not use just a foil covering.  They use a fine vermiculite barrier layer bellow the foil ontop of the substrate..  Spores and bacteria that enter hypothetically impact on the vermiculite barrier and never interact with the nutritious flour.  Since you don't have said vermiculite barrier, you should really be using a propper filter.  Not to say you can't get by with just foil with an airbox, but it's not a good idea.

The opened jar is almost certainly toast, though it's vaguely conceavable the mycellium will protect the uncolonized substrate under it.

Now bascillus could have any of the following causes:
Dirty inoculation procedure
Dirty inoculant
Insufficient sterilization (unlikely for a pressure cooked PF cake)
Insufficient filtering (bacteria entering from outside.)

Given that the location of the bacteria is nowhere near the edge, I would lean towards dirty inoculation procedure insufficient sterilization, or the foil.  How long did you PC and how did you PC them?  How did you inoculate?


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineMajora123
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Re: Brf lipstick mold? Can you please help me identify [Re: Machiavelliavore]
    #22350118 - 10/08/15 01:20 PM (8 years, 3 months ago)

Quote:

Machiavelliavore said:
Those grows do not use just a foil covering.  They use a fine vermiculite barrier layer bellow the foil ontop of the substrate..  Spores and bacteria that enter hypothetically impact on the vermiculite barrier and never interact with the nutritious flour.  Since you don't have said vermiculite barrier, you should really be using a propper filter.  Not to say you can't get by with just foil with an airbox, but it's not a good idea.

The opened jar is almost certainly toast, though it's vaguely conceavable the mycellium will protect the uncolonized substrate under it.

Now bascillus could have any of the following causes:
Dirty inoculation procedure
Dirty inoculant
Insufficient sterilization (unlikely for a pressure cooked PF cake)
Insufficient filtering (bacteria entering from outside.)

Given that the location of the bacteria is nowhere near the edge, I would lean towards dirty inoculation procedure insufficient sterilization, or the foil.  How long did you PC and how did you PC them?  How did you inoculate?



I placed wedges on top of the cake. So does this mean the jar wasn't sterilized right if it wet stuff just started forming their? And when a cake fully colinizes does that mean the mycelium fought off all contaims and is protected from them or can the covered jar still get contains on the mycelium?


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OfflineFreeWorldOrder
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Re: Brf lipstick mold? Can you please help me identify [Re: Majora123]
    #22350900 - 10/08/15 04:56 PM (8 years, 3 months ago)

Quote:

Majora123 said:
Quote:

Machiavelliavore said:
Those grows do not use just a foil covering.  They use a fine vermiculite barrier layer bellow the foil ontop of the substrate..  Spores and bacteria that enter hypothetically impact on the vermiculite barrier and never interact with the nutritious flour.  Since you don't have said vermiculite barrier, you should really be using a propper filter.  Not to say you can't get by with just foil with an airbox, but it's not a good idea.

The opened jar is almost certainly toast, though it's vaguely conceavable the mycellium will protect the uncolonized substrate under it.

Now bascillus could have any of the following causes:
Dirty inoculation procedure
Dirty inoculant
Insufficient sterilization (unlikely for a pressure cooked PF cake)
Insufficient filtering (bacteria entering from outside.)

Given that the location of the bacteria is nowhere near the edge, I would lean towards dirty inoculation procedure insufficient sterilization, or the foil.  How long did you PC and how did you PC them?  How did you inoculate?



I placed wedges on top of the cake. So does this mean the jar wasn't sterilized right if it wet stuff just started forming their? And when a cake fully colinizes does that mean the mycelium fought off all contaims and is protected from them or can the covered jar still get contains on the mycelium?




With all due respect, you should do some more reading on the basics. It will really help out in the long run to get a better understanding of everything.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


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InvisibleSupalemonhaze
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Re: Brf lipstick mold? Can you please help me identify [Re: Majora123]
    #22353410 - 10/09/15 07:05 AM (8 years, 3 months ago)

Quote:

These grow in the wild on top of shit so I don't know why we have to be so sanitary I guess it's because so many houses have the common green mold growing inside the homes


when they grow outside the soil or manure they grow in has a natural balance of bacteria and molds constantly fighting. This is something mushrooms have dealt with successfully over thousands of years with the help of some other bacteria/mold. When you grow indoors there isnt a balance at all thats why when you get a contam some people tell you to bury it in a flower patch or a garden because it has more chance of thriving there.

Especially when we sterilise grain/brf that substrate becomes a magnet of sorts to bacteria and mold since it is so uninhabited.

Good luck with your grow


Edited by Supalemonhaze (10/09/15 07:20 AM)


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OfflineDrCrumbs
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Re: Brf lipstick mold? Can you please help me identify [Re: Supalemonhaze]
    #22353747 - 10/09/15 09:12 AM (8 years, 3 months ago)

Yeah your no longer growing on a selective medium, and environment. Lots of stuff love wet brown rice flour left in the dark.

What was your procedure?

If your working with agar, you may want to use something like PC'ed grains. So you can toss them around, or a liquid culture.


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