Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDinkinFlicka84
Dude
Male User Gallery


Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
It's day 8 after I shook MS syringe from reputable vendor. WTF?
    #22349793 - 10/08/15 11:49 AM (8 years, 3 months ago)

I realize now to not shake a MS after inoculation.
Will it still colonize? Should I re-inoculate with agar of the same strai? I've never waited this long for it to start.

It's already been PCd for 2 hrs.


--------------------


Extras: Filter Print Post Top
InvisibleJacobStorm
psychedelic cartel
I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
    #22349808 - 10/08/15 11:53 AM (8 years, 3 months ago)

I'm sorry what ecatly happened?


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


Extras: Filter Print Post Top
OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: JacobStorm]
    #22349897 - 10/08/15 12:18 PM (8 years, 3 months ago)

You injected spores and then shook the jar? Somewhere you read that your not suppose to shake ms because you want the spores to meet and start growing? But realistically you shot like hundreds of thousands of spores in there and even tho you shook two of the spores are gonna meet. Instead of having thousands of spores in one small area you have them spread out so it wont be so concentrated so to speak. You'll notice small bits of growth spread out instead of one big spot. I'm sure your gonna be fine its just gonna take longer.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


Extras: Filter Print Post Top
OfflineMachiavelliavore
Vermiculite Hater
 User Gallery


Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
    #22349898 - 10/08/15 12:18 PM (8 years, 3 months ago)

Yes it will still colonize.

There's been some debate about whether shaking MS immediately is good.

No shaking has the advantage that spores stay closer together and hypothetically are more likely to germinate.  You can also see what germinates more clearly when it's on the sides of the jar, and a better idea of the colonized volume for a second shake timing.

People that shake after MS say there are so many spores it doesn't matter if you distribute them and you're wasting time by not doing so.

I think the one thing we all agree on is there is not a massive difference.

8 days is absolutely not atypical.  Had that from some fresh prints.



I'm assuming it was not PC'd after inoculation right :smile:


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
Offlineshroombasa
Sojourner
 User Gallery


Registered: 05/02/15
Posts: 364
Loc: 'Merka
Last seen: 3 years, 3 months
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
    #22350082 - 10/08/15 01:10 PM (8 years, 3 months ago)

I'm also a little confused.

Did you PC after you inoculated?  If so, you just sterilized your spores.

If you inoculated after you PC'ed, then it really shouldn't matter than you shook your jars.  The advantage in not shaking is that you'll see quicker growth, but provided your syringe is clean, it shouldn't make a difference.


--------------------
Trade list



Peace and long life.


Extras: Filter Print Post Top
OfflineDinkinFlicka84
Dude
Male User Gallery


Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: Machiavelliavore]
    #22351335 - 10/08/15 06:27 PM (8 years, 3 months ago)

Quote:

Machiavelliavore said:
Yes it will still colonize.

There's been some debate about whether shaking MS immediately is good.

No shaking has the advantage that spores stay closer together and hypothetically are more likely to germinate.  You can also see what germinates more clearly when it's on the sides of the jar, and a better idea of the colonized volume for a second shake timing.

People that shake after MS say there are so many spores it doesn't matter if you distribute them and you're wasting time by not doing so.

I think the one thing we all agree on is there is not a massive difference.

8 days is absolutely not atypical.  Had that from some fresh prints.



I'm assuming it was not PC'd after inoculation right :smile:



Some fresh prints of bell aire?

Good to know. Will this take even longer to get 100% colonization that if not shaken? By 8 days, my G2G have been 100% for a couple of days by now. Even my agar is at about 20% or more by day 8. I remember using MS and not shaking, which took about 2 weeks fully colonize, so I'm wondering if shaking will take more than 2 weeks to colonize.

My agar jars look like these:
Are they ready for a shake? I am hard at judging what is 20-30%.


--------------------


Extras: Filter Print Post Top
OfflineDinkinFlicka84
Dude
Male User Gallery


Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: shroombasa]
    #22351371 - 10/08/15 06:33 PM (8 years, 3 months ago)

Quote:

shroombasa said:
I'm also a little confused.

Did you PC after you inoculated?  If so, you just sterilized your spores.

If you inoculated after you PC'ed, then it really shouldn't matter than you shook your jars.  The advantage in not shaking is that you'll see quicker growth, but provided your syringe is clean, it shouldn't make a difference.




Sorry, I should've been more specific. I was just mentioning at the end of my post that I PC for 2 hours to show that I doubt my WBS was contaminated to start, so that I could understand why my MS hadn't shown any growth yet in my jars that were inoculated long after being PC'd.


--------------------


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: Machiavelliavore]
    #22352151 - 10/08/15 09:32 PM (8 years, 3 months ago)

Quote:

Machiavelliavore said:
Yes it will still colonize.

There's been some debate about whether shaking MS immediately is good.





not much to debate though....your forgetting a key part of this is to keep an eye on contams, hard to spot them if you tossing all your solution everywhere and the thousands of spores is a dumb arguement


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineMachiavelliavore
Vermiculite Hater
 User Gallery


Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: cronicr]
    #22353478 - 10/09/15 07:29 AM (8 years, 3 months ago)

I actually mentioned all those things. ^


OP, anyway, you know MS will always take longer to get growing than a live mycellium inoculant.  However, compared to a wedge, the MS will germinate in a much large area.

The MS germination may be a bit more difficult to spot in a shake jar as well.

As far as shaking your MS jars again, that's just going to be a judgement call based on how well the spore solution was distributed.  Similar to deciding if you should shake a g2g twice if you didn't use enough grain.


You could shake those agar jars nows.  I might be inclinded to wait a couple more days.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
OfflineDinkinFlicka84
Dude
Male User Gallery


Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: Machiavelliavore]
    #22354228 - 10/09/15 11:08 AM (8 years, 3 months ago)

Quote:

Machiavelliavore said:
I actually mentioned all those things. ^


OP, anyway, you know MS will always take longer to get growing than a live mycellium inoculant.  However, compared to a wedge, the MS will germinate in a much large area.

The MS germination may be a bit more difficult to spot in a shake jar as well.

As far as shaking your MS jars again, that's just going to be a judgement call based on how well the spore solution was distributed.  Similar to deciding if you should shake a g2g twice if you didn't use enough grain.


You could shake those agar jars nows.  I might be inclinded to wait a couple more days.




It's always been tough for me to decide what 20% colonization looks like. However, when I see the agar in those jars look that thick and hearty, I feel that it will recover just fine, despite the fact that it's not 20% just yet.


--------------------


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
    #22354237 - 10/09/15 11:10 AM (8 years, 3 months ago)

Quote:

DinkinFlicka84 said:
Quote:

Machiavelliavore said:
I actually mentioned all those things. ^


OP, anyway, you know MS will always take longer to get growing than a live mycellium inoculant.  However, compared to a wedge, the MS will germinate in a much large area.

The MS germination may be a bit more difficult to spot in a shake jar as well.

As far as shaking your MS jars again, that's just going to be a judgement call based on how well the spore solution was distributed.  Similar to deciding if you should shake a g2g twice if you didn't use enough grain.


You could shake those agar jars nows.  I might be inclinded to wait a couple more days.




It's always been tough for me to decide what 20% colonization looks like. However, when I see the agar in those jars look that thick and hearty, I feel that it will recover just fine, despite the fact that it's not 20% just yet.



don't think about it too much, if there is a fair bit colonized give it a shake


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* I am scared to do a Print/syring!
( 1 2 all )
fearforce7 9,049 27 12/30/21 08:54 AM
by Forrester
* Re: ELECTION - Spore Vendor Forum
( 1 2 3 all )
Russdogg 4,928 40 04/12/01 02:18 PM
by Thor
* Wtf should i do? Vulture 632 14 07/13/02 05:32 PM
by DaxMiddler
* WTF????? MsPacMan 645 9 11/26/02 05:22 AM
by DERRAYLD
* Improper Pasturization of Compost...
( 1 2 all )
celsius 3,937 33 08/05/01 04:27 PM
by BrownPastures
* how to store a syrings? UlisSausage7 2,332 2 10/11/05 08:46 AM
by Marx
* best spore vendor?
( 1 2 3 all )
gray1 44,192 44 08/26/17 10:33 AM
by ThirdEyeHigh
* Re: Is SporeWorks very reputable????? Anonymous 2,833 3 05/19/00 02:19 AM
by Mycelium5150

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
508 topic views. 30 members, 203 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.009 seconds on 14 queries.