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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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It's day 8 after I shook MS syringe from reputable vendor. WTF?
#22349793 - 10/08/15 11:49 AM (8 years, 3 months ago) |
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I realize now to not shake a MS after inoculation. Will it still colonize? Should I re-inoculate with agar of the same strai? I've never waited this long for it to start.
It's already been PCd for 2 hrs.
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
#22349808 - 10/08/15 11:53 AM (8 years, 3 months ago) |
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I'm sorry what ecatly happened?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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PabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: JacobStorm]
#22349897 - 10/08/15 12:18 PM (8 years, 3 months ago) |
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You injected spores and then shook the jar? Somewhere you read that your not suppose to shake ms because you want the spores to meet and start growing? But realistically you shot like hundreds of thousands of spores in there and even tho you shook two of the spores are gonna meet. Instead of having thousands of spores in one small area you have them spread out so it wont be so concentrated so to speak. You'll notice small bits of growth spread out instead of one big spot. I'm sure your gonna be fine its just gonna take longer.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
#22349898 - 10/08/15 12:18 PM (8 years, 3 months ago) |
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Yes it will still colonize.
There's been some debate about whether shaking MS immediately is good.
No shaking has the advantage that spores stay closer together and hypothetically are more likely to germinate. You can also see what germinates more clearly when it's on the sides of the jar, and a better idea of the colonized volume for a second shake timing.
People that shake after MS say there are so many spores it doesn't matter if you distribute them and you're wasting time by not doing so.
I think the one thing we all agree on is there is not a massive difference.
8 days is absolutely not atypical. Had that from some fresh prints.
I'm assuming it was not PC'd after inoculation right
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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shroombasa
Sojourner



Registered: 05/02/15
Posts: 364
Loc: 'Merka
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
#22350082 - 10/08/15 01:10 PM (8 years, 3 months ago) |
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I'm also a little confused.
Did you PC after you inoculated? If so, you just sterilized your spores.
If you inoculated after you PC'ed, then it really shouldn't matter than you shook your jars. The advantage in not shaking is that you'll see quicker growth, but provided your syringe is clean, it shouldn't make a difference.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: Machiavelliavore]
#22351335 - 10/08/15 06:27 PM (8 years, 3 months ago) |
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Quote:
Machiavelliavore said: Yes it will still colonize.
There's been some debate about whether shaking MS immediately is good.
No shaking has the advantage that spores stay closer together and hypothetically are more likely to germinate. You can also see what germinates more clearly when it's on the sides of the jar, and a better idea of the colonized volume for a second shake timing.
People that shake after MS say there are so many spores it doesn't matter if you distribute them and you're wasting time by not doing so.
I think the one thing we all agree on is there is not a massive difference.
8 days is absolutely not atypical. Had that from some fresh prints.
I'm assuming it was not PC'd after inoculation right 
Some fresh prints of bell aire?
Good to know. Will this take even longer to get 100% colonization that if not shaken? By 8 days, my G2G have been 100% for a couple of days by now. Even my agar is at about 20% or more by day 8. I remember using MS and not shaking, which took about 2 weeks fully colonize, so I'm wondering if shaking will take more than 2 weeks to colonize.
My agar jars look like these: Are they ready for a shake? I am hard at judging what is 20-30%.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: shroombasa]
#22351371 - 10/08/15 06:33 PM (8 years, 3 months ago) |
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Quote:
shroombasa said: I'm also a little confused.
Did you PC after you inoculated? If so, you just sterilized your spores.
If you inoculated after you PC'ed, then it really shouldn't matter than you shook your jars. The advantage in not shaking is that you'll see quicker growth, but provided your syringe is clean, it shouldn't make a difference.
Sorry, I should've been more specific. I was just mentioning at the end of my post that I PC for 2 hours to show that I doubt my WBS was contaminated to start, so that I could understand why my MS hadn't shown any growth yet in my jars that were inoculated long after being PC'd.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: Machiavelliavore]
#22352151 - 10/08/15 09:32 PM (8 years, 3 months ago) |
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Quote:
Machiavelliavore said: Yes it will still colonize.
There's been some debate about whether shaking MS immediately is good.
not much to debate though....your forgetting a key part of this is to keep an eye on contams, hard to spot them if you tossing all your solution everywhere and the thousands of spores is a dumb arguement
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: cronicr]
#22353478 - 10/09/15 07:29 AM (8 years, 3 months ago) |
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I actually mentioned all those things. ^
OP, anyway, you know MS will always take longer to get growing than a live mycellium inoculant. However, compared to a wedge, the MS will germinate in a much large area.
The MS germination may be a bit more difficult to spot in a shake jar as well.
As far as shaking your MS jars again, that's just going to be a judgement call based on how well the spore solution was distributed. Similar to deciding if you should shake a g2g twice if you didn't use enough grain.
You could shake those agar jars nows. I might be inclinded to wait a couple more days.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: Machiavelliavore]
#22354228 - 10/09/15 11:08 AM (8 years, 3 months ago) |
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Quote:
Machiavelliavore said: I actually mentioned all those things. ^
OP, anyway, you know MS will always take longer to get growing than a live mycellium inoculant. However, compared to a wedge, the MS will germinate in a much large area.
The MS germination may be a bit more difficult to spot in a shake jar as well.
As far as shaking your MS jars again, that's just going to be a judgement call based on how well the spore solution was distributed. Similar to deciding if you should shake a g2g twice if you didn't use enough grain.
You could shake those agar jars nows. I might be inclinded to wait a couple more days.
It's always been tough for me to decide what 20% colonization looks like. However, when I see the agar in those jars look that thick and hearty, I feel that it will recover just fine, despite the fact that it's not 20% just yet.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: It's day 8 after I shook MS syringe from reputable vendor. WTF? [Re: DinkinFlicka84]
#22354237 - 10/09/15 11:10 AM (8 years, 3 months ago) |
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Quote:
DinkinFlicka84 said:
Quote:
Machiavelliavore said: I actually mentioned all those things. ^
OP, anyway, you know MS will always take longer to get growing than a live mycellium inoculant. However, compared to a wedge, the MS will germinate in a much large area.
The MS germination may be a bit more difficult to spot in a shake jar as well.
As far as shaking your MS jars again, that's just going to be a judgement call based on how well the spore solution was distributed. Similar to deciding if you should shake a g2g twice if you didn't use enough grain.
You could shake those agar jars nows. I might be inclinded to wait a couple more days.
It's always been tough for me to decide what 20% colonization looks like. However, when I see the agar in those jars look that thick and hearty, I feel that it will recover just fine, despite the fact that it's not 20% just yet.
don't think about it too much, if there is a fair bit colonized give it a shake
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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