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OfflineIfishhigh
Stranger

Registered: 03/21/15
Posts: 570
Last seen: 4 years, 6 months
Another pf tek?
    #22349241 - 10/08/15 09:18 AM (8 years, 3 months ago)

I have a clear box with arm holes cut out. The thing is I don't have tyvec arm sleeves. Is it absolutely necessary? I know this sounds amateur but can I have my wife wrap plastic wrap Round my arms? A freshly cleaned long sleeve shirt will do ? Or do I suck it up and order some tyvec sleeves off if Amazon ? Any responses or advice will be appreciated.

Thanks


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InvisibleBigfeely123
Stranger
Registered: 01/30/15
Posts: 2,594
Re: Another pf tek? [Re: Ifishhigh]
    #22349263 - 10/08/15 09:25 AM (8 years, 3 months ago)

The tyvec sleeves are not an absolute must.


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OfflineIshida
Decisive Gambler
Male

Registered: 07/15/15
Posts: 73
Last seen: 8 years, 1 month
Re: Another pf tek? [Re: Bigfeely123]
    #22349287 - 10/08/15 09:33 AM (8 years, 3 months ago)

Just clean your self thourghly and put on a clean long sleeve cotton shirt. Be sure to scrub your arms thoroughly tho.


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OfflineIfishhigh
Stranger

Registered: 03/21/15
Posts: 570
Last seen: 4 years, 6 months
Re: Another pf tek? [Re: Ishida]
    #22349380 - 10/08/15 09:56 AM (8 years, 3 months ago)

I have hairy arms so I would definetly put on a kind sleeve. I know this isn't the right forum but I posted this question in the contamination forum and it's prettyslow over there. Am I being cheap ? Or is the tyvec something a lot of people skip ?


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OfflineHanz
Freak & Gentleman
Male


Registered: 08/02/15
Posts: 2,932
Loc: Amsterdam
Last seen: 5 years, 4 months
Re: Another pf tek? [Re: Ifishhigh]
    #22349443 - 10/08/15 10:13 AM (8 years, 3 months ago)

I used new, fresh plastic bags from a roll. Cut out the bottoms, stick my arms through and have someone else tape the plastic to my latex gloves. Not even sure this was necessary, but it worked for me. Contam rate was acceptable. Good luck!

Love, Hanz.


--------------------
Small scale alternative parties rich in empathy and extravagance. Happen to know of one in the vicinity of Amsterdam? PM me my dear fellow. I love to meet some other freaks.

Oh and, if you can,.. embrace the nyctomorph. It needs you.


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InvisibleBigfeely123
Stranger
Registered: 01/30/15
Posts: 2,594
Re: Another pf tek? [Re: Ifishhigh] * 1
    #22349455 - 10/08/15 10:16 AM (8 years, 3 months ago)

Try 'Mushroom Cultivation' forums.


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OfflineIfishhigh
Stranger

Registered: 03/21/15
Posts: 570
Last seen: 4 years, 6 months
Re: Another pf tek? [Re: Bigfeely123]
    #22349707 - 10/08/15 11:18 AM (8 years, 3 months ago)

Thanks . Bags is a good idea. Sorry beefy


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OfflineRebelutionsssss
Mdmazing
I'm a teapot User Gallery


Registered: 07/23/14
Posts: 13,137
Loc: San Francisco
Last seen: 2 years, 2 months
Re: Another pf tek? [Re: Ifishhigh]
    #22349753 - 10/08/15 11:34 AM (8 years, 3 months ago)

Just use trash bags and wipe the outside down with rubbing alcohol. Rubber bands around your wrists


--------------------
:
To define is to confine.


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InvisibleChemicalSpark

Registered: 10/08/15
Posts: 2,057
Re: Another pf tek? [Re: Rebelutionsssss]
    #22350211 - 10/08/15 01:50 PM (8 years, 3 months ago)

.


Edited by ChemicalSpark (03/23/20 07:18 PM)


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InvisibleBigfeely123
Stranger
Registered: 01/30/15
Posts: 2,594
Re: Another pf tek? [Re: ChemicalSpark]
    #22350219 - 10/08/15 01:51 PM (8 years, 3 months ago)

FOAF? You mean you..? Only time will tell Sparky.


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OfflineIfishhigh
Stranger

Registered: 03/21/15
Posts: 570
Last seen: 4 years, 6 months
Re: Another pf tek? [Re: Bigfeely123]
    #22350463 - 10/08/15 03:01 PM (8 years, 3 months ago)

I had to google foaf

( friend of a friend )


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OfflineHanz
Freak & Gentleman
Male


Registered: 08/02/15
Posts: 2,932
Loc: Amsterdam
Last seen: 5 years, 4 months
Re: Another pf tek? [Re: ChemicalSpark] * 1
    #22350662 - 10/08/15 04:01 PM (8 years, 3 months ago)

Quote:

ChemicalSpark said:
forgot to make sure the 16gauge needle would fit in the holes on the jar lids... So they had to clean a nail with isopropyl and make the holes larger before being able to inoculate.




AFAIK isopropyl does not really sterilize, it just sanitizes a little. You should have used a flame. Only flames really kill. Besides, the contam could also be on the outside of the lids, so, in theory, pushing a sterile nail through a dirty hole could also contam the substrate.

Hey, but I don't want to sound negative :lol: .. I wish you the best, and hope everything works out!

:heart: Hanz.


--------------------
Small scale alternative parties rich in empathy and extravagance. Happen to know of one in the vicinity of Amsterdam? PM me my dear fellow. I love to meet some other freaks.

Oh and, if you can,.. embrace the nyctomorph. It needs you.


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