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OfflinePhantoma
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Registered: 10/07/15
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Last seen: 1 year, 8 months
First-timer and trying Panaeolus Cyan
    #22347084 - 10/07/15 07:23 PM (8 years, 3 months ago)

Howdy All!

I feel I followed the PFTEK quite closely, inoculating with syringes I filled with a bought spore print. I used a flow hood and haven't seen any contamination but they stopped growing in the jars about 2 weeks ago and I the inoculation was almost a month back. I had nickel and quarter sized white splotches a week after inoc., now they are at the most 50 cent sized irregular growths. I used 1 1/2 cups vermiculite, 3/4 cup manure, 2 tbsp brown rice flour, 3/8 cup water. Jars were turned tightly. Temp has been most often 73-78° with a couple spikes and dips.
My question is, of course, what do I do? Is there any hope to get them on track? Are they dead? Can you transfer to the terrarium and case them in the dark? What do these babies need?!!! I don't want to go to huge efforts if this is a lost cause.
Any wisdom or links are/is much appreciated!

I'm going to be a 1st time dad soon. I want to have some success with this first!  :wink:

-Phantoma


--------------------
"The mind which plunges into Surrealism, relives with burning excitement the best part of childhood."

"Objects seen in dreams should be manufactured and put on sale."

-Andre Breton


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InvisibleMad Season
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Re: First-timer and trying Panaeolus Cyan [Re: Phantoma]
    #22347153 - 10/07/15 07:37 PM (8 years, 3 months ago)

Quite brave doing pan cyan to start. Pics would definitely help.


--------------------
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Improve your sterile techniques! (A comprehensive guide to agar)
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OfflinecronicrFacebook
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Re: First-timer and trying Panaeolus Cyan [Re: Mad Season]
    #22347156 - 10/07/15 07:38 PM (8 years, 3 months ago)

:begger:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

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OfflinePhantoma
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Re: First-timer and trying Panaeolus Cyan [Re: cronicr]
    #22347275 - 10/07/15 07:57 PM (8 years, 3 months ago)

Here are three sample jars from 9 total. Mostly the growths are looking brownish which makes me think they ain't so alive, but a few splotches are still pretty white!




--------------------
"The mind which plunges into Surrealism, relives with burning excitement the best part of childhood."

"Objects seen in dreams should be manufactured and put on sale."

-Andre Breton


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Re: First-timer and trying Panaeolus Cyan [Re: Phantoma]
    #22347350 - 10/07/15 08:07 PM (8 years, 3 months ago)

Im actually thinking the mycelium seems pretty healthy in 3rd pic. Could it be the dark brown is from condensation cuz I have read that growing mycelium gives off a lot of heat?

Edit: oops meant 3rd pic

I am a newb like yourself so im curious if I am right. Now I gotta wait a few mins to find out if Im told to "gtfo u funghi breath narb" or not:thumbup:


Edited by Supalemonhaze (10/07/15 08:22 PM)


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InvisibleMad Season
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Re: First-timer and trying Panaeolus Cyan [Re: Phantoma]
    #22347379 - 10/07/15 08:12 PM (8 years, 3 months ago)

What was your sterilization procedure and inoculation techniques? They don't look too good imo. First looks dark/wet. 2nd looks moldy, never seen pan myc do that, and 3rd looks bacterial.

Have you considered testing your spores on agar and inoculating with something started from a clean culture?


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleSupalemonhaze
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Re: First-timer and trying Panaeolus Cyan [Re: Mad Season]
    #22347443 - 10/07/15 08:25 PM (8 years, 3 months ago)

Quote:

Mad Season said:
First looks dark/wet. 2nd looks moldy, never seen pan myc do that, and 3rd looks bacterial.



 

0.0 see im not worth listening to just yet hence my moodsign
<-- :adventuretime:


Edited by Supalemonhaze (10/07/15 08:28 PM)


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Invisibleeatyualive
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Re: First-timer and trying Panaeolus Cyan [Re: Supalemonhaze]
    #22347453 - 10/07/15 08:26 PM (8 years, 3 months ago)

:popcorn:


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OfflinePhantoma
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Re: First-timer and trying Panaeolus Cyan [Re: Mad Season]
    #22347479 - 10/07/15 08:30 PM (8 years, 3 months ago)

Mad Season,
What shows up as yellow or green in the photo is the gold color of the vermiculite. I don't know that I can get any better photos, but I assure you there are no black, yellow or green growths.

I was quite anally careful in following sterilization procedures. Used a flow hood, latex gloves, lots of isopropyl alcohol, a flame for the needle. The needle had been filled with boiled water. I used pond water in the substrate but that and the rest of the ingredients were pasteurized in a covered pot for an hour while in their hole-taped 1/2 pint jars. Etc, etc.

I don't have any spores left over, unfortunately. I don't know anything about agar methods yet.


--------------------
"The mind which plunges into Surrealism, relives with burning excitement the best part of childhood."

"Objects seen in dreams should be manufactured and put on sale."

-Andre Breton


Edited by Phantoma (10/07/15 08:39 PM)


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InvisibleSupalemonhaze
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Re: First-timer and trying Panaeolus Cyan [Re: Phantoma]
    #22347543 - 10/07/15 08:38 PM (8 years, 3 months ago)

Thats unlucky. If you were to try again buy some agar cuz its the best way to ensure you put a clean sample in you jars so that limits contamination and helps you pinpoint where the contams actually came from.

Not to mention the added bonus of making a clean LC (liquid culture) and being able to choose a good rhizo ( a stingy type of mycelium which is said to be faster growing) strain which supposedly increases yield. It's a lotta fun too


Edited by Supalemonhaze (10/07/15 08:40 PM)


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InvisibleMad Season
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Re: First-timer and trying Panaeolus Cyan [Re: Phantoma]
    #22347546 - 10/07/15 08:38 PM (8 years, 3 months ago)

I'm not saying there's any black or green growths :smile:. That's poo/verm right? For the first pic it just looked thin and weak as well as dark. That's why I speculate it's too wet.

Second pic has a brown tinge inside the mycelium. Granted that could be verm. So a better pic would say for sure.

And third pic is definitely bacterial. Pan myc shouldn't have metabolites/browning at this stage. Pan myc should be fluffy and aggressive.

Imo pan myc is more aggressive than cubes on manure, so it should be much quicker. I noticed you say you only pasteurized.. maybe you should pc them. Or even just do the steam/boil for 90 minutes. Pasteurization imo isn't a good enough Sterilization for jars. Even if there is manure in them.

If you decide to let them chill and see how they go, I'd highly highly highly recommend getting cubensis spores in the mean time. You won't regret it.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisibleeatyualive
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Re: First-timer and trying Panaeolus Cyan [Re: Phantoma]
    #22347578 - 10/07/15 08:43 PM (8 years, 3 months ago)

sometimes pan mycelia looks off. its different on dung. something like this colonized.



even on plates its a little strange sometimes.



oats tiger dropped



this is what it looks like when its overgrown by about 2-3 weeks.


hope that helps. when the jars get further along it will be a little easier to tell. yellow isn't good. but grey/white is normal.this thread has some good pics for reference.

http://www.shroomery.org/forums/showflat.php/Number/22338439#22338439


--------------------
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:flame::chief:JOIN THE POW WOW:chief::flame:


Edited by eatyualive (10/07/15 08:51 PM)


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InvisibleSupalemonhaze
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Re: First-timer and trying Panaeolus Cyan [Re: eatyualive]
    #22347633 - 10/07/15 08:53 PM (8 years, 3 months ago)

Quote:

eatyualive said:

even on plates its a little strange sometimes.






Is that blue agar? 0.0 never saw that before


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OfflinePhantoma
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Re: First-timer and trying Panaeolus Cyan [Re: Supalemonhaze]
    #22347759 - 10/07/15 09:24 PM (8 years, 3 months ago)

Thanks, Mad Season. Embarrassed to say, but, I thought I was getting Psilocybe Cyanescens. Didn't know Panaeolus Cyanescens was different but figured it out after I got the spores. Definitely cubensis next time, now that it's clear I'm not a child prodigy.
Do you think I should loosen the lid on the jars to release some moisture?

Eatyualive, those images are definitely helpful, as is the link! I will make my decision after reading up a little.

{Bowing to you both}


--------------------
"The mind which plunges into Surrealism, relives with burning excitement the best part of childhood."

"Objects seen in dreams should be manufactured and put on sale."

-Andre Breton


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InvisibleGoblinKing
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Re: First-timer and trying Panaeolus Cyan [Re: Supalemonhaze]
    #22347962 - 10/07/15 10:22 PM (8 years, 3 months ago)

Quote:

Supalemonhaze said:
Quote:

eatyualive said:

even on plates its a little strange sometimes.






Is that blue agar? 0.0 never saw that before





Food coloring is used to make spotting contams easier.


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