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Invisibletahoe
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Are these jars ready for their first shaking? If not now, when?
    #22345195 - 10/07/15 12:42 PM (8 years, 3 months ago)

I have been seeing a lot of post on when to shake the jars. People saying outrageous things like 1/3, 1/2, 25%-50%. Also read things about not shaking more than once or twice.

Let's make sure we all know what these percentages look like. These jars were inoculated with agar wedges on 9/30. What would you call this jar? I would say ten percent. Maybe a tad higher/lower. Are these jar ready to shake?




The jars above are the same jars. There are random growths of mycelium because o rolled the jars around a bit when I first added the wedges to get the wedges situated under some grain.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlineorison
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Re: Are these jars ready for their first shaking? If not now, when? [Re: tahoe]
    #22345273 - 10/07/15 12:56 PM (8 years, 3 months ago)

sometimes when doing a few more than one jar,, Ill shake one and leave the rest, if the shake recovers, then its safe to shake the others. 25% .. jmo


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Offlinetetherface
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Re: Are these jars ready for their first shaking? If not now, when? [Re: tahoe]
    #22345279 - 10/07/15 12:57 PM (8 years, 3 months ago)

I would shake them.... when using wedges as inoculate I normally wouldn't have let them go quite that far before my first shake


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Invisibleblackdust
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Re: Are these jars ready for their first shaking? If not now, when? [Re: tetherface]
    #22345325 - 10/07/15 01:08 PM (8 years, 3 months ago)

those jars are fucked
filled with bacteria


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Invisibletahoe
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blackdust]
    #22345423 - 10/07/15 01:24 PM (8 years, 3 months ago)

Quote:

blackdust said:
those jars are fucked
filled with bacteria




Are you serious?



So are people waiting to shake because they are watching for contamination?

Here are two more jars that were wedged at the same time. These have gone through one round of shaking on 10/3. They are going to get shook up again now. I am pretty sure that they will be done colonizing before the other jars.



--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleblackdust
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Re: Are these jars ready for their first shaking? If not now, when? [Re: tahoe]
    #22345444 - 10/07/15 01:28 PM (8 years, 3 months ago)

those two jars look good.
we shake to check for contam.
if no or slow recover = bad

of course bacteria jars can do okay with a high spawn ration but will contam out after first flush


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Invisibletahoe
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blackdust]
    #22345597 - 10/07/15 01:54 PM (8 years, 3 months ago)

Quote:

blackdust said:
those two jars look good.
we shake to check for contam.
if no or slow recover = bad

of course bacteria jars can do okay with a high spawn ration but will contam out after first flush




I am pretty sure that shaking jars is meant to speed up colonization by spreading out the mycelium. It's not done to check for contamination. But who knows what has been taught while I have been gone


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleblackdust
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Re: Are these jars ready for their first shaking? If not now, when? [Re: tahoe]
    #22345623 - 10/07/15 01:59 PM (8 years, 3 months ago)

well yeah, shake at 30% and then your jar should be fully colonized within a week if not days if its actually clean. Especially when using WBS or millet as the grain


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OfflinecronicrFacebook
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blackdust]
    #22345690 - 10/07/15 02:10 PM (8 years, 3 months ago)

We shake to speed colonization up, most do this at 30, for years I was a multiple shake type of person but since I expand my grain a lot further these days I do it once per jar


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I'm tired do me a favor


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Invisibletahoe
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Re: Are these jars ready for their first shaking? If not now, when? [Re: cronicr]
    #22345857 - 10/07/15 02:43 PM (8 years, 3 months ago)

I could see shaking twice when going g2g. Shake it once when the grain is first added. That would put you a week ahead of using an agar wedge. Then a second shake 3-5 days later.
That absolutely makes sense.

This isn't directed at you cronicr.

It doesn't make sense to me to wait past 10% colonization when using agar, and possibly spore syringe or li. You want to get that mycelium growing on as much grain as possible as soon as possible. The mycelium grows three dimensionally. Pieces of grain in the center of a colonized two inch ball are not doing anything. It's better to get those broken up and out doing their job.

Of course there is to much shaking too soon. By too much I mean you don't want to shake daily. But you do want to give it Hell on the days you do shake. The purpose is to break up the ball of mycelium and spread it out. Do whatever it takes.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflinecronicrFacebook
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Re: Are these jars ready for their first shaking? If not now, when? [Re: tahoe]
    #22346522 - 10/07/15 05:11 PM (8 years, 3 months ago)

i used to shake at about 10% and again at 50% as seen by folks like citric and had wicked colonizing times but iwasnt' g2g'ing then,now that i am though breaking up my poor myc that much doens't sit well with me


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleblackdust
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Re: Are these jars ready for their first shaking? If not now, when? [Re: cronicr]
    #22346531 - 10/07/15 05:14 PM (8 years, 3 months ago)

Quote:

cronicr said:
i used to shake at about 10% and again at 50% as seen by folks like citric and had wicked colonizing times but iwasnt' g2g'ing then,now that i am though breaking up my poor myc that much doens't sit well with me



are the jars in the op bacterial or just overly wet?


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Invisibleblindingleaf
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blackdust]
    #22346566 - 10/07/15 05:24 PM (8 years, 3 months ago)

just a little on the moist side or the GE hole is small.

I'm with tahoe, i shake at the same time he does in his first pics, and then again around 50%.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflinePinPornProducer
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Re: Are these jars ready for their first shaking? If not now, when? [Re: tahoe]
    #22346637 - 10/07/15 05:43 PM (8 years, 3 months ago)

Quote:

tahoe said:
I have been seeing a lot of post on when to shake the jars. People saying outrageous things like 1/3, 1/2, 25%-50%. Also read things about not shaking more than once or twice.

Let's make sure we all know what these percentages look like. These jars were inoculated with agar wedges on 9/30. What would you call this jar? I would say ten percent. Maybe a tad higher/lower. Are these jar ready to shake?




The jars above are the same jars. There are random growths of mycelium because o rolled the jars around a bit when I first added the wedges to get the wedges situated under some grain.



From my experience, excessive condensation in jars is the first sign of a contamination :shrug:


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Invisibleblackdust
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Re: Are these jars ready for their first shaking? If not now, when? [Re: PinPornProducer]
    #22346653 - 10/07/15 05:46 PM (8 years, 3 months ago)

thats been my experience too
but im just a noob :sad:


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Invisibleblindingleaf
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blackdust]
    #22346658 - 10/07/15 05:48 PM (8 years, 3 months ago)

its early on in the jars post PC life :crazy:

the condensation is not surrounding the mycelium.  if it was, i would agree with u guys.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Invisibleblackdust
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blindingleaf]
    #22346680 - 10/07/15 05:53 PM (8 years, 3 months ago)

thats good then
'
:awethumb:


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Invisibleblindingleaf
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blackdust]
    #22346738 - 10/07/15 06:10 PM (8 years, 3 months ago)

i think the GE hole might be small or the foil on top is blocking partially maybe?
it is thick around the new growth, like bacteria would be, but too early to tell for certain.
for now, i think it is clean and that thickness might be due to lack of ideal GE.

but that just like…my opinion man :blazed:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Invisibleblackdust
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blindingleaf]
    #22346773 - 10/07/15 06:20 PM (8 years, 3 months ago)

makes since man
lack of GE does make myc puffy


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InvisibleKalistis
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Re: Are these jars ready for their first shaking? If not now, when? [Re: blackdust]
    #22347374 - 10/07/15 08:11 PM (8 years, 3 months ago)



I shook these last night and they recovered quickly. They were inoculated on 9/23 with Malabar MS and were shaken a little prematurely at like 15% to 20%. At the recommendation of a few TC's I shook them again last night at 80 to 90% with 4 completely colonized. I did not shake the completely colonized ones.


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