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OfflineLand TroutM
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Re: Psilocybin mushroom potency study is about to begin [Re: Mr Piggy] * 2
    #28077839 - 12/01/22 11:07 AM (1 year, 1 month ago)

:retrocool:


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InvisibleDandurn777
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Re: Psilocybin mushroom potency study is about to begin [Re: Mr Piggy]
    #28077885 - 12/01/22 11:38 AM (1 year, 1 month ago)

Lol. Government web crawlers be advised. These prints are for “microscopy” only. No prints were harmed in the making of this message.


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Prying open my Allenii




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Offlineknarkkorven
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Re: Psilocybin mushroom potency study is about to begin [Re: Dandurn777] * 1
    #28077918 - 12/01/22 11:57 AM (1 year, 1 month ago)

So I found the zip with the supplementary data and could confirm that my samples was included.



My Pholiotina is described in the quote below. Unfortunatly as P. cyanopus. DNA is pointing more to P. smithii.
https://www.shroomery.org/forums/showflat.php/Number/14895064/

Quote:

A very interesting and rare species in our dataset is Pholiotina cyanopus, for which we
analyzed three fruiting bodies from a single fungarium collection. P. cyanopus contained
0.821–1.360 mg/g BA, 0.247–0.565 mg/g NB, less than or equal to 0.062 mg/g PS, and less
than or equal to 0.859 mg/g PSB. Our results were very similar to the previous study by
Halama et al. [13], who also reported a trace concentration of AE.




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OfflineLand TroutM
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Re: Psilocybin mushroom potency study is about to begin [Re: knarkkorven]
    #28078105 - 12/01/22 01:26 PM (1 year, 1 month ago)

Did you collect cultures and work with them at all?  Neat little fungi.


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Offlineknarkkorven
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Re: Psilocybin mushroom potency study is about to begin [Re: Land Trout] * 2
    #28078252 - 12/01/22 02:36 PM (1 year, 1 month ago)

No, I haven't performed any cultivation the last 15 years except spreading Psilocybe semilanceata spore water in good habitats (with success).


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OfflineAlan RockefellerM
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Re: Psilocybin mushroom potency study is about to begin [Re: knarkkorven] * 3
    #28078883 - 12/01/22 10:33 PM (1 year, 1 month ago)

Quote:

knarkkorven said:
The long-awaited study is finally here!
This must be it, right? Alan, can you confirm?




Yes that's it!

Also a new study is being done.  Samples of species not represented here are needed.  The authors are discussing the best way to receive samples - perhaps they will be sent directly to the forensic laboratory.  I should have an address for people to send samples in the next few days.


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InvisibleIcyurmt
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Re: Psilocybin mushroom potency study is about to begin [Re: Alan Rockefeller]
    #28078897 - 12/01/22 10:51 PM (1 year, 1 month ago)

:popcorn:


--------------------
👁️ 🌊 why you are empty.

Hunt for the habitat not the mushroom.


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Offlineleschampignons
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Re: Psilocybin mushroom potency study is about to begin [Re: knarkkorven]
    #28080160 - 12/02/22 06:38 PM (1 year, 1 month ago)

This is fascinating. Thank you for sharing.


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leschampignons Trade List


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OfflineMoria841
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Re: Psilocybin mushroom potency study is about to begin [Re: leschampignons]
    #28080410 - 12/02/22 08:52 PM (1 year, 1 month ago)

Wait, P. fimetaria INACTIVE??


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Moria's Gymnopilus Guide


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InvisibleDandurn777
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Re: Psilocybin mushroom potency study is about to begin [Re: Moria841]
    #28080438 - 12/02/22 09:05 PM (1 year, 1 month ago)

Seems as though they postulate there may be two fimentaria… one inactive and one active.


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Prying open my Allenii




Edited by Dandurn777 (12/02/22 09:06 PM)


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OfflineDH42
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Re: Psilocybin mushroom potency study is about to begin [Re: Moria841] * 1
    #28081378 - 12/03/22 01:54 PM (1 year, 1 month ago)

Quote:

Moria841 said:
Wait, P. fimetaria INACTIVE??





Only two collections were sampled, not enough to conclude that the species is inactive. More research is needed (and is going on)


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Have a look at the subreddit r/fimetaria!


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InvisibleMr Piggy
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Re: Psilocybin mushroom potency study is about to begin [Re: DH42]
    #28081412 - 12/03/22 02:07 PM (1 year, 1 month ago)

I'm going to be honest, this study does not have a large enough sample group for most of the species sampled to be considered valid in any way.  It's great in concept but most of the results cannot be taken in good faith.


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🅃🄴🄰🄼 🄵🄾🄸🄻


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OfflineLand TroutM
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Re: Psilocybin mushroom potency study is about to begin [Re: Mr Piggy]
    #28081473 - 12/03/22 02:46 PM (1 year, 1 month ago)

“Our results for all tryptamines in P. subaeruginosa were very low (the highest of 0.33 mg/g PS) and did not correspond much to those found in other species of the P. cyanescens complex. We speculate that this species may produce much higher tryptamine concentrations and additional analyses are therefore desired.”
This surprised me, but I wonder too, if there is just so much diversity within that species.


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InvisibleDandurn777
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Re: Psilocybin mushroom potency study is about to begin [Re: Land Trout]
    #28082099 - 12/03/22 08:37 PM (1 year, 1 month ago)

It would be curious to see what sample size is necessary to give an adequate overall overview of the particular species. Like subs have so many different phenotypes, and I wonder if there’s potency differences between phenos, or if there’s potency differences based on soil type, topography, location, substrate, etc. I wonder if there’s variation within cyanescens for different states on the west coast, or places in Europe, etc? Hmmm 🤔


--------------------
Prying open my Allenii




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OfflineHeyowana
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Re: Psilocybin mushroom potency study is about to begin [Re: Dandurn777] * 1
    #28082475 - 12/04/22 03:18 AM (1 year, 1 month ago)

Yes P.subaeruginosa potency varies greatly in NSW. Pine forest subs are weakest. Mountain subs in eucalypt forests are more potent. Coastal subs from the lowlands fruit later and are nearly always more psychedelic than mountain subs.

I was picking mutant Psilocybe cyanescens in Sydney 1981-88. No sub I have ever picked came close for potency. Been picking P.cyanescens last two seasons in parkland. Same goes for them. There's just no comparison. To me they are like the LSD trip of the mushroom world. Once tried never the same.

Sure I'm bio assaying them. I have a fair bit of experience with Australian species. P.papuana is right up there in potency. However dried it's not the same. Quite boring and lacking the distinctive visuals of fresh specimens.

I was very pleased to read this paper. Hope it leads to bigger and better things.


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Offlineknarkkorven
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Re: Psilocybin mushroom potency study is about to begin [Re: Heyowana]
    #28082765 - 12/04/22 09:57 AM (1 year, 1 month ago)

The fimetaria was not bluing according to the authors.

Quote:

Although one of the P. fimetaria collections was analyzed only a few months after
sampling (PS-65), we were unable to detect any of the analyzed tryptamines. This collection
(PRM 951396) was found in the Czech Republic and was primarily determined according
to the morphological characters. The phylogenetic analysis shows its affinity to P. pelliculosa
(Figure 3), a psychotropic species known from the USA [29]. One of the ITS rDNA sequences
Int. J. Mol. Sci. 2022, 23, 14068 8 of 16
of P. pelliculosa was even 100% similar to that of P. fimetaria, but their EF1-a gene sequences
are apparently distinct (the sequences HF912341 and LR760712 show a match of only
94%). This would suggest that the ITS rDNA molecular marker is not always a reliable
character for species delimitation in Psilocybe (which is also indicated in the Psilocybe
cyanescens complex). According to the literature [70,71], Psilocybe fimetaria turns blue when
bruised, however, the bluing reaction was not observed in the Czech collection. Because
no other sequences of P. fimetaria are available in public databases, we were unable to
discuss the taxonomic identity of our collection. The absence of tryptamine alkaloids in
our samples could be explained, for example, by (i) low initial concentration and following
degradation before analysis, and (ii) an existence of two Psilocybe species corresponding
to the morphological concept of P. fimetaria, one psychotropic and one non-psychotropic,
as not only the bluing reaction but also PSB was reported in P. fimetaria [33]. Therefore,
a thorough analysis and multi-gene sequencing of additional collections of P. fimetaria
are needed.




And RenegadeMycologist pointed out in this thread
https://www.shroomery.org/forums/showflat.php/Number/27603287#27603287
that this specific collection did not fit in well with the other fimetara gene sequences.

Quote:

MN901955 submitted by Jan Borovička I don't know what it is, might be mislabeled liniformans.




But is perhaps something else since it's not active.


However it seems like a lot of the samples are very low on the psychoactive tryptamines.
I. aeruginascens was 10-100 times lower than other studies, P. subaeruginosa and P. caerulescens also very low.
All of mine as well. I dried my mushrooms and stored them in the freezer just until sending them to Prague, and they were kept dark in 20C until being analyzed. I never noticed a low potency. The cinctulus for example I find is almost as potent as P. cubensis.
I don't know if there is some kind of analytic errors, the methods described seems good...


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OfflineAnglerfishM
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Re: Psilocybin mushroom potency study is about to begin [Re: DH42]
    #28082855 - 12/04/22 10:51 AM (1 year, 1 month ago)

Quote:

DH42 said:
Quote:

Moria841 said:
Wait, P. fimetaria INACTIVE??





Only two collections were sampled, not enough to conclude that the species is inactive. More research is needed (and is going on)




It is actually beyond me that this is called an "extensive collection". 4 specimens of P. fimetaria, 18 specimens of P. semilanceata etc.

Several species included in the study are likely fruiting in the vast millions world wide each year.
All honours to the people doing the study, but my impression is that it falls short with regards to the amount of samples.


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Offlineknarkkorven
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Re: Psilocybin mushroom potency study is about to begin [Re: Anglerfish]
    #28082939 - 12/04/22 11:38 AM (1 year, 1 month ago)

Yes. I was hoping for a lot more people to contribute. This forum have all the enthusiasts from all over the world who are able to collect a lot of different species, including the more rare ones. Where else but on the Shroomery would a majority of all active species be collected by various people during a year + a few really interesting species a year that might not have a name yet, or haven't been been tested in quantitative studies before?

On the other hand, it also needs the people who conduct the study to be just as good to reach out for samples, updating about progress etc. Keeping the interest alive. The samples was analyzed during 2016-2019. From 2017 this thread was dead and even when it was created it quickly disappeared among all the thousands of "found this mushroom, is it magic?" threads...

It could have been done better.

But after 7 years of waiting I'm still happy to see the result.


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OfflineLand TroutM
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Re: Psilocybin mushroom potency study is about to begin [Re: knarkkorven]
    #28082968 - 12/04/22 11:50 AM (1 year, 1 month ago)

No this is very cool.  It’s a good start.  I’d be happy to contribute to future studies.


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OfflineAnglerfishM
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Re: Psilocybin mushroom potency study is about to begin [Re: knarkkorven] * 1
    #28083357 - 12/04/22 02:43 PM (1 year, 1 month ago)

Quote:

knarkkorven said:
Yes. I was hoping for a lot more people to contribute. This forum have all the enthusiasts from all over the world who are able to collect a lot of different species, including the more rare ones. Where else but on the Shroomery would a majority of all active species be collected by various people during a year + a few really interesting species a year that might not have a name yet, or haven't been been tested in quantitative studies before?

On the other hand, it also needs the people who conduct the study to be just as good to reach out for samples, updating about progress etc. Keeping the interest alive. The samples was analyzed during 2016-2019. From 2017 this thread was dead and even when it was created it quickly disappeared among all the thousands of "found this mushroom, is it magic?" threads...

It could have been done better.

But after 7 years of waiting I'm still happy to see the result.




I guess it comes down to time and resources. Mycological studies are rarely funded unless there is some larger
program organised or eventually commercial or monetary interests. Exploration of psychedelic fungi is not (yet)
a priority, so it boils down to volunteer work, and little to no economical repair for those who put in the effort
and long hours. Maybe a funding campaign could work.


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