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Offlineirhavok
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Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags?
    #22344381 - 10/07/15 08:52 AM (8 years, 3 months ago)

Bags have been hanging for about 8 hours now, in hindsight I am not confident in the way I sealed the bags. The WBS is still hydrated.

I cleaned the hell out of a small bathroom, lysol'd and then pulled the bags out of the PC to seal them, but I am certain one the of the bags opened a little more than I'd have liked or that I am reading is acceptable in other threads.

I realize now i should have hand sealed them right inside the PC but I didn't. I did however as stated do this in a very clean room. I did not use gloves or a mask either but I did thorough wash myself first. Basically feel like i just dropped the ball at the end of a long day and I need some confirmation.

Any help is appreciated.


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Invisibletahoe
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: irhavok]
    #22344491 - 10/07/15 09:36 AM (8 years, 3 months ago)

Yes you can pc them again. I do it every once in awhile. I have even done it to grain that smelled yeasty. You might notice some darkening of the grain after a second cook. Don't worry about moisture, the grain will be close enough.

And for sealing the bags. I and many others do so before cooking. I take a clump of polyfill, close the bag around it and zip tie it in. I pc with the bag zip tied up. Then I inoc by sticking a syringe through the side up top. And cover the syringe hole with masking tape as I remove the needle.

Here is a reused bag/grain. The old masking tape survived pcing also.



--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlineirhavok
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: tahoe]
    #22344567 - 10/07/15 09:58 AM (8 years, 3 months ago)

Can we use a hand sealer to seal the bag the same as you're doing with a ziptie/polyfil?

Our bags have filter patches, so could i just fold them neatly, seal them and put them into the PC?


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Invisibletahoe
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: irhavok]
    #22344818 - 10/07/15 10:57 AM (8 years, 3 months ago)

What is a hand sealer? The battery operated type is just about useLess.
You couldn't seal the bag and use polyfill. But I am not really sure what you mean by sealing the same as I do with poly and zip tie.

Depends on filter micron. Some bags can tolerate being sealed and pc'ed. I use the .2 micron, they will pop if sealed and pc'ed.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlineirhavok
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: tahoe]
    #22344892 - 10/07/15 11:20 AM (8 years, 3 months ago)

Yes, I was using a battery operated hand sealer as recommended in many TEKs on here. Are you suggesting its garbage?


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Invisibletahoe
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: irhavok]
    #22345006 - 10/07/15 11:58 AM (8 years, 3 months ago)

The three different battery operated sealers I have seen were junk. But maybe yours is better. The one that I used sealed a line this thick -------. I prefer a sealer that seals at least an 1/8th thick.

But I still do not know how you plan on using polyfill and sealing a bag with a sealer


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinemushpunx
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: irhavok]
    #22345014 - 10/07/15 12:03 PM (8 years, 3 months ago)

ow did you pack the bags into the pressure cooker? Did you fold up a tyvek sleeve into it or just put them in folded up and un sealed?

The inside of the pressure cooker is not sterile, it pulls dirty air inside as it cools, which can pull it into your bags.
Most of us  accordian fold a tyvek sleeve between the gussets on the bags so it acts as a filter.

When the PC has been allowed to cool all the way the spawn bags should look almost vacuum sealed. As long as they are sealed they are OK to leave out in open air untill you open them in your still air box or in front of a flow hood.



Here is some spawn bags folded up with tyvek sleeves before they have gone thru PC cycle

When its time to i nnoculate the bags, you wipe them down thoroughly with ISO and do it in a still air box or in front of a flow hood. Do not try to do it in open air no matter how much you bleach the room, you need still air or laminar flow.

  Inoculate the bag however, and inflate it with sterile air from the flow hood to give a good plenum. This is the downside of working with spawn bags without one, in a still air bag you cant give much of a plenum because you'll just let dirty air into the bag. That and you need a big still air box otherwise its going to be hard to work with.

  The picture at the top is an impulse sealer, unless you have a good one of these I wouldn't try to seal the bag. Fold the top and twist and use zip ties or tie wire instead! The spawn bags in the photo Iposted are done with twist ties.


RR's lestgrowmushroom videos has a great section on using spawn bags, and another great section on innoculating them. I think its the chapter sealing with sawdust

Most spawn bags will burst or leak if you try to pre seal them before your PC run. In any case its difficult to achieve with the typical spawn bag with filter patch if you use a sealer

So learn to fold them with tyvek and pack the PC proper, and learn to use a still air box with proper sterile technique. A flow hood or SAB is a *MUST* for spawn bags.


 


--------------------

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Edited by mushpunx (10/07/15 12:40 PM)


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OfflineFreeWorldOrder
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: tahoe]
    #22345112 - 10/07/15 12:25 PM (8 years, 3 months ago)

Quote:

tahoe said:
Yes you can pc them again. I do it every once in awhile. I have even done it to grain that smelled yeasty. You might notice some darkening of the grain after a second cook. Don't worry about moisture, the grain will be close enough.

And for sealing the bags. I and many others do so before cooking. I take a clump of polyfill, close the bag around it and zip tie it in. I pc with the bag zip tied up. Then I inoc by sticking a syringe through the side up top. And cover the syringe hole with masking tape as I remove the needle.

Here is a reused bag/grain. The old masking tape survived pcing also.






Interesting post on sealing bags... I like it. Going to try some bags out in the near future.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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Offlinemushpunx
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: tahoe]
    #22345172 - 10/07/15 12:36 PM (8 years, 3 months ago)

Quote:

tahoe said:
Yes you can pc them again. I do it every once in awhile. I have even done it to grain that smelled yeasty. You might notice some darkening of the grain after a second cook. Don't worry about moisture, the grain will be close enough.

And for sealing the bags. I and many others do so before cooking. I take a clump of polyfill, close the bag around it and zip tie it in. I pc with the bag zip tied up. Then I inoc by sticking a syringe through the side up top. And cover the syringe hole with masking tape as I remove the needle.

Here is a reused bag/grain. The old masking tape survived pcing also.








This is really cool man! With the spawn bags that have filter patches, most of them burst if you impulse seal the top before PC cycle.

I've really been wanting to pre seal my bags to just inject with LI

So the polyfill is a really cool idea! Right now I i nnoculate with master jars via G2G. But I just made a blender attachment to turn my plates into liquid innoculant and I would rather just inject it in rather than pouring it in the top


--------------------

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Edited by mushpunx (10/07/15 12:42 PM)


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Invisibletahoe
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: mushpunx]
    #22345275 - 10/07/15 12:56 PM (8 years, 3 months ago)

I don't use a hood or box when inoculating my bags. Keep it mind that the bags are sealed with a zip tie and polyfill. You will need a hood or box if you plan on opening the bag.

The first thing I do when I get a spore syringe, or make a mycelium syringe, is put a 22-23 gauge needle on it. Smaller hole in the bag. Then I pick an injection point up high near the polyfill. I do not pay the extra money for the bags with injection port. (I think it's a bad idea to stick a needle into the grain of multiple bags.) I wipe the bag with alcohol, then apply a strip of masking tape. Pull back the tape a bit and then sick the needle through the bag and inject. Then as I pull out the needle, I lay the tape back over the hole. I have had no problem with contams.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinemushpunx
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Re: Can I re-pressure cook WBS if I am not confident in the way I sealed my Bags? [Re: tahoe]
    #22345324 - 10/07/15 01:08 PM (8 years, 3 months ago)

Yea man that's why wanna give your idea a shot! My house is full of spores, even with my flow hood and pulling out all the stops its hard to keep them out of my work. So Im gunna change tactics for a while cause I think it gets in during my G2G innoculations.

My agar work is pretty spot on, so as long as I make a clean LI I should be golden!


--------------------

Amateur Mycologists United
AMU Q&A


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