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Offlinewow012345
photographer

Registered: 10/07/15
Posts: 32
Last seen: 8 years, 27 days
it is Contamination?
    #22344234 - 10/07/15 07:30 AM (8 years, 3 months ago)

1.
i  have grow kit. it start colonization. and i find some grean dot in grow kit. it is mold? i think it can be mold.  (you can see big pic!)

2.
and one of my container have a moister. small is not that bad but it is not that look good. it is sign of contamination? (you can see big pic!)

3.
i uses rye seed. i shake my jar. i can observe. it became little bit sticky
it is nomal? it is sign of colonization? or contamination?

( sorry about my shitty English. i hate my self hahaha) if you correct my sentence i will appreciate that)


is this post came from pub board. sorry about that.


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Edited by wow012345 (10/11/15 07:35 AM)


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: it is Contamination? [Re: wow012345]
    #22344335 - 10/07/15 08:28 AM (8 years, 3 months ago)

Yeah first is something like trich. Could be aspergillus or penicillium.. we just need a microscope to say for sure.

Second is bacteria(bacillus), aka wet spot.

I notice there's foil on the lids. Can I ask why? Grain jars aren't supposed to have foil on them because it can ruin the integrity of the minimum .3 micron filter. The filter will keep things out when you shake and what not. If you don't use a filter... I suggest you get on that. Poke a hole in the lid and stuff it tight with polyfil if you have to. It's better than nothing.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Offlinewow012345
photographer


Registered: 10/07/15
Posts: 32
Last seen: 8 years, 27 days
Re: it is Contamination? [Re: Mad Season]
    #22344973 - 10/07/15 11:48 AM (8 years, 3 months ago)

Quote:

Mad Season said:
Yeah first is something like trich. Could be aspergillus or penicillium.. we just need a microscope to say for sure.

Second is bacteria(bacillus), aka wet spot.

I notice there's foil on the lids. Can I ask why? Grain jars aren't supposed to have foil on them because it can ruin the integrity of the minimum .3 micron filter. The filter will keep things out when you shake and what not. If you don't use a filter... I suggest you get on that. Poke a hole in the lid and stuff it tight with polyfil if you have to. It's better than nothing.





thank you for your answer.
ok, that mean l lost almost of my container. get ucked by wet spot.

my english is not that good.
and i`m new to Mycology. so i can`t understan what you mean perfectly.

i understand first and sececond paragraph. but i cant not understand third paragraph.
1.i have few container,foil on the lids. should i remove foil?
2.i just isolate container,contaminated by wet spot. i cant not save my container?


what should i do. please tell me what is your suggestion.
and also could you see my other container and jar? please.

1.
it is my big jar (900ml)
i want to save this jar so badly. i made this jar 5days ago.
it was covered by aluminium foil
( i just remove aluminium foil and attach aluminium  tape for plugged up the hole,
my jar is not that dry but grain have a moisture little bit.(before sterilize, i sock grain 12 hour)  this jar get ucked by wet point too?
maybe this jar contaminated by wet spot very little bit. (i am not sure) in that case can i save this jar?


Edited by wow012345 (10/11/15 07:36 AM)


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OfflineCHeifM4sterDiezL
Chief Globerts


Registered: 07/28/10
Posts: 22,541
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Re: it is Contamination? [Re: wow012345]
    #22344993 - 10/07/15 11:55 AM (8 years, 3 months ago)



this looks fucked you guys really think thats wet spot?


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Offlinewow012345
photographer


Registered: 10/07/15
Posts: 32
Last seen: 8 years, 27 days
Re: it is Contamination? [Re: CHeifM4sterDiezL]
    #22345093 - 10/07/15 12:22 PM (8 years, 3 months ago)

Quote:

CHeifM4sterDiezL said:


this looks fucked you guys really think thats wet spot?




persona ly that is not wet spot.  may be it can be mold. i can not sure.

i think it is wet spot (different container)


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OfflineAchillita
Back to the basics
Male


Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 10 days
Re: it is Contamination? [Re: wow012345]
    #22345157 - 10/07/15 12:34 PM (8 years, 3 months ago)

Instead of Aluminum tape, try anad obtain Micropore tape. Or polyfill. The mushrooms needs a filter so they can breathe, but still block out contaminates.


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OfflineCHeifM4sterDiezL
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Registered: 07/28/10
Posts: 22,541
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Last seen: 18 minutes, 7 seconds
Re: it is Contamination? [Re: Achillita]
    #22345198 - 10/07/15 12:43 PM (8 years, 3 months ago)

oh yeh that shit is funky i thought maybe u jus didnt rinse ur grains enough


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
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Re: it is Contamination? [Re: wow012345]
    #22345529 - 10/07/15 01:43 PM (8 years, 3 months ago)

Quote:

Achillita said:
Instead of Aluminum tape, try anad obtain Micropore tape. Or polyfill. The mushrooms needs a filter so they can breathe, but still block out contaminates.



:whathesaid: 4 pieces of MP tape or polyfil stuffed tight in the hole is much MUCH better than anything aluminum. You only need aluminum for the pressure cooker, to keep the filter dry during the cycle.

Quote:

wow012345 said:
Quote:

CHeifM4sterDiezL said:


this looks fucked you guys really think thats wet spot?




persona ly that is not wet spot.  may be it can be mold. i can not sure.

i think it is wet spot (different container)




First one is contaminated as well. Looks like penicillium to me :P. Second one is of course wet spot.

All contaminated containers can be saved. Soap and water to clean them, and when you pressure cook the next batch, all your contams in the container will die.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinewow012345
photographer


Registered: 10/07/15
Posts: 32
Last seen: 8 years, 27 days
Re: it is Contamination? [Re: Mad Season]
    #22346463 - 10/07/15 04:57 PM (8 years, 3 months ago)

Thx guys, i will get Micropore tape ASAP
Grow shrooms are not that easy.    If i will success cultivating shroom, it can not possible without you guys help. Once again thank you for your advice!



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Edited by wow012345 (10/11/15 07:36 AM)


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