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Offlinetedoro
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Proper storage proceedure for a syringe of grain LI
    #22341009 - 10/06/15 12:59 PM (8 years, 3 months ago)

I have been making Grain LI by injecting sterile water into rye berry myco bags and sucking up the cloudy water.

What is the best procedure to store the syringes? I am using 60mm syringes. I also bought syringe air filters that fit the syringe if you remove the needle.

Could I refrigerate the Grain LI with some airspace at the top, and put on the filter, storing it upright? Maybe do that needle/filter swap in a still air box? Or is there a better way that I am not thinking of. I just want them to stay strong for a few months...

I ask because some syringes I stored in the fridge for a month were very weak when I used them (I also didn't let them come back to room temp) I am suspicious they might desire some air.

Many thanks!

T


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Offlinetedoro
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Re: Proper storage proceedure for a syringe of grain LI [Re: tedoro]
    #22342380 - 10/06/15 07:35 PM (8 years, 3 months ago)

Well, I'll go with this procedure, unless anyone has more to add...


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InvisibleMad Season
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Re: Proper storage proceedure for a syringe of grain LI [Re: tedoro]
    #22343653 - 10/07/15 12:21 AM (8 years, 3 months ago)

It's because of the lack of nutrients. Storage of stuff with no nutrients causes it to go dormant. Thus people use li's pretty much when they make them. You can always just store grain masters or plates in the fridge? LI them when you need them


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Edited by Mad Season (10/07/15 12:21 AM)


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Offlinemidnightmaraude
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Re: Proper storage proceedure for a syringe of grain LI [Re: Mad Season]
    #22344359 - 10/07/15 08:43 AM (8 years, 3 months ago)

There's no nutrients in the water from the grain??

Would mixing in some karo help?


Edited by midnightmaraude (10/07/15 08:44 AM)


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InvisibleMad Season
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Re: Proper storage proceedure for a syringe of grain LI [Re: midnightmaraude]
    #22344373 - 10/07/15 08:48 AM (8 years, 3 months ago)

Sounds like a way to make it contaminate... Just get into agar work. So much better all around.


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Re: Proper storage proceedure for a syringe of grain LI [Re: Mad Season]
    #22344420 - 10/07/15 09:12 AM (8 years, 3 months ago)

not if you add it before the sterilization process.
agar is agar and has its benefits but for those looking for fast growth, well I don't need to tell you what's what.


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Re: Proper storage proceedure for a syringe of grain LI [Re: midnightmaraude]
    #22344436 - 10/07/15 09:16 AM (8 years, 3 months ago)

Add karo to a jar of grains before Sterilization? Or are you saying to sterilize in a syringe with karo? Dude either way it isn't good because it's a syringe of nutrients taking in mycelium from a grain jar. Grains which are high in endospores. Grains which are almost impossible to tell if they're clean.. if 1 single bacterium gets into your syringe you're straight fucked.

AGAR WILL TELL YOU IF IT'S CLEAN FIRST. THATS THE POINT.

If you want your liquids to have nutrients you have to get it from a clean agar plate if you want it to be successful almost all the time


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Edited by Mad Season (10/07/15 09:24 AM)


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Offlinetedoro
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Re: Proper storage proceedure for a syringe of grain LI [Re: Mad Season]
    #22346479 - 10/07/15 05:01 PM (8 years, 3 months ago)

I was dreaming of keeping some around for 4 weeks. Do you think I can achieve that?

t


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Invisibleeatyualive
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Re: Proper storage proceedure for a syringe of grain LI [Re: tedoro]
    #22347428 - 10/07/15 08:22 PM (8 years, 3 months ago)

i tend to store in a dorm fridge. but others have mentioned they have stored them in room temps with success. i still have a liquid culture syringe from the works from 2007 that has fruited continually since then using a drop of the syringe. the last grow i did with that same syringe is this one. blue and pearl oysters. i only really need one drop to grow whatever i need. so i can use that one syringe until it dies. its not as vigorous for sure, but it still fruits for personal dinner a few times.
i usually store them with the syringe tip on, maybe even electrical tape around that in a closed ziplock bag in the dorm fridge.

http://www.shroomery.org/forums/showflat.php/Number/21744280


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Offlinetedoro
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Re: Proper storage proceedure for a syringe of grain LI [Re: eatyualive]
    #22350089 - 10/08/15 01:13 PM (8 years, 3 months ago)

Cool. I like hearing they can last a while. I do wonder about air... do people leave some air in the syringe? I am hesitant to use my syringe filters on the syringe... seems like a contam possibility... the swapping of the needle on and off.

And I'm also wondering about the "resting" after the making of the GLC that some speak of. Is that good? And how long would one warm the syringes before using to noc a grain bag?

Thanks everyone! I'm so full of questions~ You all are the best.

T


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Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
Invisibleeatyualive
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Re: Proper storage proceedure for a syringe of grain LI [Re: tedoro]
    #22351475 - 10/08/15 06:51 PM (8 years, 3 months ago)

Quote:

tedoro said:
Cool. I like hearing they can last a while. I do wonder about air... do people leave some air in the syringe? I am hesitant to use my syringe filters on the syringe... seems like a contam possibility... the swapping of the needle on and off.

And I'm also wondering about the "resting" after the making of the GLC that some speak of. Is that good? And how long would one warm the syringes before using to noc a grain bag?

Thanks everyone! I'm so full of questions~ You all are the best.

T



i do not leave air in the syringe. ideally blended tissue from agar would be better. im always skeptical about storing any liquid from grains or anything like that more than a week. agar plate or stem blended up store a good amount of time.
if you store for a long period of time there is a shelf life. and you will definitely see loss of vigor. but you can always put it back on a plate and transfer.


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Offlinetedoro
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Re: Proper storage proceedure for a syringe of grain LI [Re: eatyualive]
    #22351791 - 10/08/15 08:08 PM (8 years, 3 months ago)

Thanks eats... Thats exactly the experience I'm trying to gain without all the lost time.  I'm now leaning toward slowing the master bag down in the fridge and using the GLC immediately.

t


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
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