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InvisibleSupalemonhaze
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Registered: 10/02/15
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Is this bad news? Agar
    #22339993 - 10/06/15 07:40 AM (8 years, 3 months ago)

So I poured agar yesterday and put them in an empty terrarium with a heating mat under it. Now I have a little pool of water in my plates. I searched and I know the little condensation spots arent a prob but I am curios if this is too much



Edited by Supalemonhaze (10/06/15 07:42 AM)


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InvisibleSupalemonhaze
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Re: Is this bad news? Agar [Re: Supalemonhaze]
    #22339999 - 10/06/15 07:43 AM (8 years, 3 months ago)

Why is photo so small 0.0


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InvisibleFantastic Mr. Fox
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Re: Is this bad news? Agar [Re: Supalemonhaze]
    #22340198 - 10/06/15 08:53 AM (8 years, 3 months ago)

upload it to the site.

You can pour off standing water using an SAB.

Stop using heating mats & leave everything at room tempature (74°-77°F)


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Giving is all we have, for we're just grateful to be alive

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InvisibleSupalemonhaze
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Re: Is this bad news? Agar [Re: Fantastic Mr. Fox]
    #22340306 - 10/06/15 09:33 AM (8 years, 3 months ago)

Oh I though optimal incubation temp is 86. Anyway if its gonna be filling my plates like that I dont wanna use it anyway


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InvisibleKalistis
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Re: Is this bad news? Agar [Re: Supalemonhaze]
    #22340392 - 10/06/15 10:07 AM (8 years, 3 months ago)

My plates blow up at 75 degrees F and my jars got metabolites the second I turned my air off and house went over 80 degrees. I turned the air back on in a matter of a couple days the metabolites were gone.

It seems that if I pour my agar too hot than I get too much condensation but it usually transfers through the stack of plates to the one sitting on top. I still use the plate, but usually for transfers that are less than ideal so I don't feel  so bad if things don't work out.

At work, we incubate dermatophytes at room temp but anything bacterial at warmer temps.


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InvisibleSupalemonhaze
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Re: Is this bad news? Agar [Re: Kalistis]
    #22342562 - 10/06/15 08:04 PM (8 years, 3 months ago)

Yes mine too were stacked inside my terrarium. Now ive tried putting napkins under a few un inoculated plates to see how they do. So far they are ok. Wow I put my plates in my pressure cooker to sterilise them when I did my agar. Thank god they didnt blow up. How do you sterilise ypurs then? At 75degrees they are bound to have something on them right?


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InvisibleKalistis
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Re: Is this bad news? Agar [Re: Supalemonhaze]
    #22342618 - 10/06/15 08:18 PM (8 years, 3 months ago)

Do you have Pyrex Petri dishes? Otherwise, how did you not melt your plates in the PC. What temps and pressure are you running your PC at?

I purchase sterilized, single use, Petri dishes. I mix my agar in a Pyrex container that can hold 500ml with a lid then I PC for 20 minutes at 15 PSI. Let the agar come close to room temp (able to hold glass jar without a glove but still in liquid form) then pour in my SAB. I let them sit in their sleeve overnight then move them into a ziplock until I am ready to inoculate them.

Once inoculated I wrap the edge in Parafilm

I haven't had a terrible time with contaminants but I have gotten 2 or 3 dirty plates which I think came from my technique putting the lid back on after inoculation.


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InvisibleKalistis
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Re: Is this bad news? Agar [Re: Kalistis]
    #22342649 - 10/06/15 08:24 PM (8 years, 3 months ago)

Lol... And when I said "they blow up at 75 degrees", I meant they do well.... Great mycelium growth. They don't explode.


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InvisibleHigher Love
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Re: Is this bad news? Agar [Re: Supalemonhaze]
    #22342667 - 10/06/15 08:28 PM (8 years, 3 months ago)

Build a stand at the back of your SAB. After pouring, place the petri's on it with lids and allow to cool for an hour. I have not had condensation buildup or contaminants this way


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InvisibleSupalemonhaze
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Re: Is this bad news? Agar [Re: Higher Love] * 1
    #22343807 - 10/07/15 01:31 AM (8 years, 3 months ago)

Quote:

Caelistis said:
Lol... And when I said "they blow up at 75 degrees", I meant they do well.... Great mycelium growth. They don't explode.




Oh lmao my bad. Yes they are glass I think they are called steriplan. As for pc temp and pressure I have no idea its a very old model so I dont have its specs, cant seem to find them online also. But so far im not seeing any contams on grain, pf jars and agar so I think I cook them long enough


Quote:

Higher Love said:
Build a stand at the back of your SAB. After pouring, place the petri's on it with lids and allow to cool for an hour. I have not had condensation buildup or contaminants this way




The condensation actually occured while they were in incubation. But it seems I found a way to counter it. I am no longer stacking them and I placed a piece of paper towel underneath them. I also put them upside down for whatever its worth


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InvisibleHigher Love
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Re: Is this bad news? Agar [Re: Supalemonhaze]
    #22347885 - 10/07/15 09:58 PM (8 years, 3 months ago)

Quote:

The condensation actually occured while they were in incubation. But it seems I found a way to counter it. I am no longer stacking them and I placed a piece of paper towel underneath them. I also put them upside down for whatever its worth




Good deal. Hope that works for you. Once mine cool in the box for an hour I cover them and stick them in a clean container upside down, then stick in a drawer in my fridge overnight or for a couple hours. After that I like to bring them into higher temps (~75f) for a few days to test for contams before inoculation.
Good luck and glad to see more people are using Agar work.


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InvisibleKalistis
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Re: Is this bad news? Agar [Re: Higher Love]
    #22347972 - 10/07/15 10:28 PM (8 years, 3 months ago)

Yeah, I would bet the condensation occurred because you used a heating pad under your plates. Also, how do you know your technique is adequate if you have no idea what temp or pressure your PC is running at?


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InvisibleSupalemonhaze
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Re: Is this bad news? Agar [Re: Kalistis]
    #22348545 - 10/08/15 03:11 AM (8 years, 3 months ago)

Quote:

Higher Love said:
Quote:

The condensation actually occured while they were in incubation. But it seems I found a way to counter it. I am no longer stacking them and I placed a piece of paper towel underneath them. I also put them upside down for whatever its worth




Good deal. Hope that works for you. Once mine cool in the box for an hour I cover them and stick them in a clean container upside down, then stick in a drawer in my fridge overnight or for a couple hours. After that I like to bring them into higher temps (~75f) for a few days to test for contams before inoculation.
Good luck and glad to see more people are using Agar work.




Thanks its been a lotta fun despite having inferior parafilm and having to change it a few times a day.


Quote:

Caelistis said:
Yeah, I would bet the condensation occurred because you used a heating pad under your plates. Also, how do you know your technique is adequate if you have no idea what temp or pressure your PC is running at?




Well thats why I made 3 different teks so far to see what works and what doesnt with my pc. As for sterilisation times im just winging it. I did grain jars at 2hrs pf jars at 1 hour and agar 1 hour and LC at 45 minutes I think. So far the agar and LC seem spotless and both are growing mycelium from clones rather clearly. Im thinking of this as a test run


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