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Psilosopherr
A psilly goose



Registered: 02/15/12
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Prolonging the shelf life of sclerotia producing mycelium
#22338803 - 10/05/15 09:38 PM (8 years, 3 months ago) |
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Most sclerotia growers seem to think that the stones gain more power with time. I've also read reports that around 9-12 month old jars start to risk contamination. I've also read that ph gradually goes down the longer mycelium inhabits substrate. I was thinking that perhaps one could prolong shelf life by buffering the ph against change, maybe with oyster shells. Or even opening up old jars and watering them with ph treated water. Or even a ph dunk.
Or maybe the mycelium can only last so long on the nutrition in the grain and this is all futile. But that only makes me think, why not spawn to bulk and just let that sit in non-fruiting conditions for a long time.
Shoot, I wonder if you could separate the stones from their old grains and then just use those stones as spawn/g2g or whatever to get them fresh nutritious material. I wonder if you could flame sterilize the outside of a sclerotium! They are made to resist forest fires in nature aren't they?? 
Guide my future experimenting with your two cents online mushroom community! Please?
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99.99
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: Psilosopherr]
#22338915 - 10/05/15 10:09 PM (8 years, 3 months ago) |
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Harvest the stones at 4 months and put the leftover grains in a fruiting chamber a case then in a month ore soo harvest shrooms as well as stones Put half a stones in rye and start all over
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Psilosopherr
A psilly goose



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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: 99.99]
#22338928 - 10/05/15 10:13 PM (8 years, 3 months ago) |
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yeah I'm familiar with that. Except the last bit. Are you saying people do use stones to start new rye jars with success? I wonder how careful you need to be sterility wise when harvesting stones if you plan to use them this way.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: Psilosopherr]
#22338960 - 10/05/15 10:23 PM (8 years, 3 months ago) |
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I suppose you could harvest them in your SAB, then go straight to grains with one...but you probably should throw that bad boy on an agar plate first to clean it up...right?
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Q&A US vs. THEM The more I learn, the less I know.
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Psilosopherr
A psilly goose



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Posts: 12,278
Last seen: 1 month, 10 days
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: LocN9ne]
#22338976 - 10/05/15 10:28 PM (8 years, 3 months ago) |
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Quote:
LocN9ne said: I suppose you could harvest them in your SAB, then go straight to grains with one...but you probably should throw that bad boy on an agar plate first to clean it up...right?
In a different situation yes, but I'm not looking to merely preserve the genetics. I'm looking to preserve the stone itself and get it to continue developing/gaining potency elsewhere.
harvesting in SAB and maybe put fruits in a fresh baggie until ready to use. I like it, thanks
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: Psilosopherr]
#22338987 - 10/05/15 10:31 PM (8 years, 3 months ago) |
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Does it work like that? I figured once it's removed from its environment something like autrophy would begin...
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Q&A US vs. THEM The more I learn, the less I know.
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99.99
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: LocN9ne]
#22339015 - 10/05/15 10:38 PM (8 years, 3 months ago) |
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I'm on my 3rd gen Stones are made to be harty enough to do this with
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: 99.99]
#22339043 - 10/05/15 10:44 PM (8 years, 3 months ago) |
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That's fuckin crazy... right the fuck on.
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Q&A US vs. THEM The more I learn, the less I know.
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99.99
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: LocN9ne]
#22339062 - 10/05/15 10:50 PM (8 years, 3 months ago) |
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It's kind of like cloneing a mushroom in a way
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: 99.99]
#22339427 - 10/06/15 01:02 AM (8 years, 3 months ago) |
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Quote:
99.99 said: I'm on my 3rd gen Stones are made to be harty enough to do this with
so you've had the same stone go through 3 jars of substrate?? crazy!
How are they? do you know?
I think I'm going to have to try flame sterilizing one just to see if it works
Edited by Psilosopherr (10/06/15 01:03 AM)
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99.99
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: Psilosopherr]
#22339514 - 10/06/15 02:10 AM (8 years, 3 months ago) |
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Well no idea if it was the same stone.. I just used them as starts for what I did
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: 99.99]
#22339518 - 10/06/15 02:13 AM (8 years, 3 months ago) |
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Quote:
99.99 said: Well no idea if it was the same stone.. I just used them as starts for what I did
oh right on. So essentially you have verified that my experiment is possible via experience, and I thank you for that if I didn't already
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99.99
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: 99.99]
#22339526 - 10/06/15 02:17 AM (8 years, 3 months ago) |
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Don't flame sterilize you'll kill the mycelium just pretend that you're doing a clone from a mushroom just take the stone wipe it down with alcohol Then I squish them and put them in my new substrate
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: 99.99]
#22339541 - 10/06/15 02:27 AM (8 years, 3 months ago) |
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sclerotia form in nature to survive forest fires don't they? I can sacrifice one stone to test it
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99.99
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Re: Prolonging the shelf life of sclerotia producing mycelium [Re: Psilosopherr]
#22339563 - 10/06/15 02:40 AM (8 years, 3 months ago) |
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Quote:
rbalzer said: sclerotia form in nature to survive forest fires don't they? I can sacrifice one stone to test it
I think thear in the ground and not exposed to direct heat.. Give it a try experiment
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