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InvisibleCuttwood
Male


Registered: 09/08/15
Posts: 3
First work with Agar, Fail Pics + 1 question
    #22337764 - 10/05/15 05:30 PM (8 years, 3 months ago)

So I have had success in the past growing but I wanted to step up my game and have since started playing with agar, and have yet to have any luck.

I purchased 3 MSS from a trusted vendor, and have since inoculated cube- golden teacher & B+ to grain as well as a LC both with happy myc growth.

When it comes to agar though these are my results.
http://imgur.com/a/cSxpt

All of my agar plates were poured on 9-29 with no visible contams, My golden teacher plate was knocked up 9-25, and B+ was KU 10-2.

The golden teacher plate to me is painfully obvious bacterial growth with
absolutely no mycelium growth.

The B+ also appears to have a large bacterial colony, but I am curious as to if I have cobweb mold, or myc growth in the 3o'clock to 7o'clock range? I have been searching a lot on these threads, pictures etc and still want to be certain with out a shadow of a doubt before I throw out another plate.

I also apologize for any grammar or spelling, English is not my first language.

edit- I also wanted to mention I do use a SAB, and will see in a few days if I can do a Grain to agar without getting a contam to rule out my technique as the culprit. The pictures posted I used 5 drops per plate and let the solution roll around the plate, I have since read that one drop will suffice as well as placing said drop onto a sterile cotton swab to them swab the agar has helped others.


Edited by Cuttwood (10/05/15 06:20 PM)


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OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: First work with Agar, Fail Pics + 1 question [Re: Cuttwood]
    #22337890 - 10/05/15 06:08 PM (8 years, 3 months ago)

9-29 and 9-25? Backwards? Either way it looks kinda like cobweb but I'm not 100%. Cobweb will grow very quickly. It will be tall and whispy or thin with a greyish color to it. You'll know right away if you leave that plate to grow awhile longer probably no more than 24 hours.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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InvisibleCuttwood
Male

Registered: 09/08/15
Posts: 3
Re: First work with Agar, Fail Pics + 1 question [Re: PabloSumgie]
    #22337985 - 10/05/15 06:28 PM (8 years, 3 months ago)

Quote:

PabloSumgie said:
9-29 and 9-25? Backwards? Either way it looks kinda like cobweb but I'm not 100%. Cobweb will grow very quickly. It will be tall and whispy or thin with a greyish color to it. You'll know right away if you leave that plate to grow awhile longer probably no more than 24 hours.




* I apologise I poured the plates on 9-25, and waited 4 days to ensure no contams before introducing MSS on 9-29* and okay I appreciate it, your description of tall and whispy is accurate, the growth has come off the plate growing upwards towards the roof of the petri dish, although it is not grey, I have quarantined it and will continue to monitor the results. I also appreciate your quick reply!


--------------------
"My grandmother told me magic mushrooms turned you gay. And if you're already gay, it turns you super mega gay. Just kidding, my grandmother died... From eating magic mushrooms."


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OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: First work with Agar, Fail Pics + 1 question [Re: Cuttwood]
    #22338479 - 10/05/15 08:27 PM (8 years, 3 months ago)

Ya sounds like cobweb. The grey will really come in later. But none the less quarantine and see what happens. If for nothing else but to see what it looks like when it gets bad.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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Offlineconfuzzed
Stranger
Male


Registered: 05/22/06
Posts: 298
Last seen: 3 years, 4 months
Re: First work with Agar, Fail Pics + 1 question [Re: PabloSumgie]
    #22340447 - 10/06/15 10:19 AM (8 years, 3 months ago)

Are you inoculating your agar from spores?

Some strains don't germ well on agar, and/or pH of the agar could be off also not allowing right conditions for spore germ.

Since you have success with LC I would innoc the agar with the good LC:thumbup:
The LC should be vigorous enough to allow for a transfer before things such as cobweb have time to take hold!

Keep us posted!


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InvisibleCuttwood
Male

Registered: 09/08/15
Posts: 3
Re: First work with Agar, Fail Pics + 1 question [Re: confuzzed]
    #22346111 - 10/07/15 03:33 PM (8 years, 3 months ago)

Quote:

confuzzed said:
Are you inoculating your agar from spores?

Some strains don't germ well on agar, and/or pH of the agar could be off also not allowing right conditions for spore germ.

Since you have success with LC I would innoc the agar with the good LC:thumbup:
The LC should be vigorous enough to allow for a transfer before things such as cobweb have time to take hold!

Keep us posted!




update - It was definitely cobweb mold within 24 hours of my post my entire plate was covered, and yes I was trying to innoc MSS directly to agar, I had purchased premixed agar from mycrosupply, I will try my hand at making my own agar using recipes from the archives/posts. I will attempt with my LC to agar now and hope for better results and once my bags are fully colonized I will also do direct grain to agar.


--------------------
"My grandmother told me magic mushrooms turned you gay. And if you're already gay, it turns you super mega gay. Just kidding, my grandmother died... From eating magic mushrooms."


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Offlineconfuzzed
Stranger
Male


Registered: 05/22/06
Posts: 298
Last seen: 3 years, 4 months
Re: First work with Agar, Fail Pics + 1 question [Re: Cuttwood]
    #22350425 - 10/08/15 02:55 PM (8 years, 3 months ago)

Good luck :thumbup:

Welcome to the shroomery too:rockon:!!!


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