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OfflineIngSocTHC
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Registered: 07/06/05
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Flow hood alternatives?
    #22337598 - 10/05/15 04:39 PM (8 years, 3 months ago)

Just getting back into mycology work and with this I jumped into agar/spawn bags. As I'm reading more I'm hearing the stress doing agar work w/o a flowhood causes. I would like to make something basic for the time being untill I have the time and funds to invest in a flowhood. My question is: what can I make that has a better sucsess rate than a glovebox without having to build a FH.
I was thinking something along g the li E of a hepa pushing into a tote.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


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OfflinePsilosopherr
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Re: Flow hood alternatives? [Re: IngSocTHC]
    #22337729 - 10/05/15 05:22 PM (8 years, 3 months ago)

a still air box. I think there's a link for how to build one in my sig.

technically they are better than a glove box. and you can't get much cheaper than a SAB (still air box)


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InvisibleHigher Love
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Re: Flow hood alternatives? [Re: Psilosopherr]
    #22337749 - 10/05/15 05:27 PM (8 years, 3 months ago)


Still air box. Do not attach gloves


--------------------


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OfflineIngSocTHC
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Re: Flow hood alternatives? [Re: Higher Love]
    #22338137 - 10/05/15 07:02 PM (8 years, 3 months ago)

SAB isbsufficient enough for agar and spawn bags then?


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


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InvisibleHigher Love
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Re: Flow hood alternatives? [Re: IngSocTHC]
    #22338212 - 10/05/15 07:17 PM (8 years, 3 months ago)

I do agar work and g2g in mine from the pic


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OfflinePsilosopherr
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Re: Flow hood alternatives? [Re: Higher Love]
    #22338434 - 10/05/15 08:19 PM (8 years, 3 months ago)

yeah me too. Agar and all that. Lots of people do it this way :thumbup:


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OfflineIngSocTHC
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Re: Flow hood alternatives? [Re: Psilosopherr] * 1
    #22338663 - 10/05/15 09:07 PM (8 years, 3 months ago)

Cherry, thanks guys.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


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OfflineMrcloudy
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Re: Flow hood alternatives? [Re: IngSocTHC]
    #22338981 - 10/05/15 10:29 PM (8 years, 3 months ago)

Yup you can achieve a pretty decent success rate with a SAB, mine was a bit restricting in movement and got on my nerves a bit. I much prefer working with a flowhood. They key to success is also probably more so with sterile technique than with whatever you are working in.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Invisiblemicro
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Re: Flow hood alternatives? [Re: Mrcloudy]
    #22341015 - 10/06/15 01:00 PM (8 years, 3 months ago)

Quote:

Mrcloudy said:
They key to success is also probably more so with sterile technique than with whatever you are working in.




^ This. Totally.

I just did what scientists do at the bench. If you take a Bunsen burner and work in back of the flame (without burning yourself, lol) the heat will draw in all the bad air. If you are working with agar you have to get used to opening the plates in the front and not the back (the side closest to the flame; open it as if it were Pacman).

Easier than a SAB in terms of construction but harder to get used to. If you can, you'll probably have more luck will a properly cleaned still air box.

Edit: Found this link, may be of some assistance:

http://nepenthiology.com/isolating-yeast-strains-part-1/



--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Edited by micro (10/06/15 01:47 PM)


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OfflineIngSocTHC
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Re: Flow hood alternatives? [Re: micro]
    #22341701 - 10/06/15 04:54 PM (8 years, 3 months ago)

Micro: I was under the impression that useing a flame like that to keep an area clean doesn't work. Any recent discussions you might be able to point me towards?


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Invisibleenlightened seed
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Re: Flow hood alternatives? [Re: Higher Love]
    #22343647 - 10/07/15 12:19 AM (8 years, 3 months ago)

Quote:

Higher Love said:

Still air box. Do not attach gloves





SAB such as this work very well :imo:


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InvisiblePsilocybePhilosphy
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Re: Flow hood alternatives? [Re: enlightened seed] * 1
    #22345152 - 10/07/15 12:33 PM (8 years, 3 months ago)

That SAB is badass and so easy...  I will be doing that!  I was getting a little irritated at some of the flow hood designs because they are expensive bulky and not very stealthy but a SAB is very stealthy,  cheap,  simple,  and the idea seems very effective.  A flame inside a SAB may not be the best idea right lol =p Thanks for sharing the design. -J*


--------------------
The only true wisdom is in knowing you know nothing.


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Invisibleenlightened seed
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Re: Flow hood alternatives? [Re: PsilocybePhilosphy]
    #22346241 - 10/07/15 04:03 PM (8 years, 3 months ago)

Quote:

PsilocybePhilosphy said:
That SAB is badass and so easy...  I will be doing that!  I was getting a little irritated at some of the flow hood designs because they are expensive bulky and not very stealthy but a SAB is very stealthy,  cheap,  simple,  and the idea seems very effective.  A flame inside a SAB may not be the best idea right lol =p Thanks for sharing the design. -J*




instead of a flame try isopropyl alcohol and/or oust.


Edited by enlightened seed (10/10/15 08:50 PM)


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OfflinePsilosopherr
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Re: Flow hood alternatives? [Re: enlightened seed]
    #22346661 - 10/07/15 05:48 PM (8 years, 3 months ago)

iso alcohol is a poor substitute for flame sterilization. most people on here will tell you that.

I just keep a propane torch lit on a table near my SAB. whenever I need to flame I simply pull said item out of the SAB and apply the flame.

Just gotta keep safety in mind.


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OfflineCraterBait
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Re: Flow hood alternatives? [Re: Psilosopherr]
    #22352833 - 10/09/15 12:36 AM (8 years, 3 months ago)

Still air box works, I just have an air purifying tower next to my work area. I keep my utensils in a uv-sterilizer cabinet when I need to set them down. I've been box-free for a while now and I haven't had any troubles.


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OfflineMoxyOx
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Re: Flow hood alternatives? [Re: micro]
    #22353308 - 10/09/15 06:15 AM (8 years, 3 months ago)

Micro, that's a pretty interesting link you posted. It never occurred to me that the air circulation from the flame could create a sterile radius.


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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InvisiblePsilocybePhilosphy
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Re: Flow hood alternatives? [Re: MoxyOx]
    #22355031 - 10/09/15 03:33 PM (8 years, 3 months ago)

I used his flame diagram the other day and it seemed great except i burned some arm hairs =p


--------------------
The only true wisdom is in knowing you know nothing.


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OfflineALiStOmega
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Re: Flow hood alternatives? [Re: PsilocybePhilosphy]
    #22360667 - 10/10/15 08:38 PM (8 years, 3 months ago)

this was easy as all you know ... shit. I can work agar in this too right? IngSocTHC, glad your getting back in to the craft fellow !!


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OfflineALiStOmega
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Re: Flow hood alternatives? [Re: ALiStOmega]
    #22360669 - 10/10/15 08:38 PM (8 years, 3 months ago)



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Invisibleenlightened seed
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Re: Flow hood alternatives? [Re: ALiStOmega]
    #22360727 - 10/10/15 08:52 PM (8 years, 3 months ago)

that might work.


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OfflineMoxyOx
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Re: Flow hood alternatives? [Re: enlightened seed]
    #22363260 - 10/11/15 01:11 PM (8 years, 3 months ago)

It would. Essentially it is the same as a glovebox, but it does have a larger opening than two circles.

How do you clean the device?


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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OfflineRJ Tubs 202
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Re: Flow hood alternatives? [Re: Psilosopherr]
    #22376364 - 10/13/15 11:00 PM (8 years, 3 months ago)

Quote:

rbalzer said:
iso alcohol is a poor substitute for flame sterilization. most people on here will tell you that.

I just keep a propane torch lit on a table near my SAB. whenever I need to flame I simply pull said item out of the SAB and apply the flame.

Just gotta keep safety in mind.




I'm used to using a flow hood, but recently I've been using a glove box, and once I start working, I don't want to pull out (insert dirty joke here) . . .

(paranoia is a factor)

But seriously, thanks for that comment. I needed that. It's tough to change one's habit . . .


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Invisibleenlightened seed
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Re: Flow hood alternatives? [Re: RJ Tubs 202]
    #22376384 - 10/13/15 11:06 PM (8 years, 3 months ago)

personally i like a SAB(glove box) with plexiglass on the top instead of the lid that comes with it.


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Invisibleenlightened seed
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Re: Flow hood alternatives? [Re: RJ Tubs 202]
    #22376508 - 10/13/15 11:38 PM (8 years, 3 months ago)

Quote:

RJ Tubs 202 said:
Quote:

rbalzer said:
iso alcohol is a poor substitute for flame sterilization. most people on here will tell you that.

I just keep a propane torch lit on a table near my SAB. whenever I need to flame I simply pull said item out of the SAB and apply the flame.

Just gotta keep safety in mind.




I'm used to using a flow hood, but recently I've been using a glove box, and once I start working, I don't want to pull out (insert dirty joke here) . . .

(paranoia is a factor)

But seriously, thanks for that comment. I needed that. It's tough to change one's habit . . .




paranoia sucks i used to get paranoid(in certain situations) but now i have no reason to  :pipesmoke:


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Invisiblemicro
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Re: Flow hood alternatives? [Re: IngSocTHC]
    #22391982 - 10/17/15 06:21 AM (8 years, 3 months ago)

Quote:

IngSocTHC said:
Micro: I was under the impression that useing a flame like that to keep an area clean doesn't work. Any recent discussions you might be able to point me towards?




Google?

Seriously, this is common aseptic technique.

Here is a Youtube video:



Believe Bio Rad if you can trust them.

This is common practice and can be found very easily in a search.

CLICK ME FOR A WHOLE LOT MORE

EDIT: If people keep asking this I'm gonna keep deeplinking threads and googlebomb the shit out of it even more just to sink it into your heads :V

(I'm kidding of course, well not the googlebombing part cause I find it amusing it is there on the first page already but... the facepalming part cause I really don't care)

This was another googlebomb anyway.

EDIT EDIT: Darn, no it wasn't but now it is :lol:


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Edited by micro (10/17/15 07:15 AM)


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OfflinePussyFart
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Re: Flow hood alternatives? [Re: micro]
    #22403435 - 10/19/15 12:48 PM (8 years, 3 months ago)

Bunsen burners get used in labs where bacteria is grown out and the cultures are tossed within a week.....we keep our cultures for far longer, so we need to take more precautions than that......the next best thing to a LFH is a SAB.....


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Invisiblemicro
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Re: Flow hood alternatives? [Re: PussyFart]
    #22406782 - 10/20/15 12:01 AM (8 years, 3 months ago)

so wait, i'm lost

is this an appeal to authority argument or appeal to oneself

because that is devoid of any substance whatsoever but

it IS

it IS because I SAY IT IS



appeal to posts that at least have some content :lol:

hoorah (i'm sure you get the joke :V )


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Offlinefruit
Registered: 10/22/15
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Re: Flow hood alternatives? [Re: micro]
    #22418203 - 10/22/15 12:56 PM (8 years, 3 months ago)

So how would one prepare a Sab for use then? Lysol and wait? Alcohol wipedown?


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OfflineMoxyOx
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Re: Flow hood alternatives? [Re: fruit]
    #22418681 - 10/22/15 02:55 PM (8 years, 3 months ago)

Reduce the amount of contaminant in the air and the work area.

So spray the environment down with lysol and wipe the SAB with alcohol.

The most important process is your technique though, fidgeting and moving quickly and hastily will fuck you over more than anything.


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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Offlinefruit

Registered: 10/22/15
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Re: Flow hood alternatives? [Re: MoxyOx]
    #22418977 - 10/22/15 04:15 PM (8 years, 3 months ago)

thanks. didnt know if there might be any special attention it should get or not


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Invisibledreadhed
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Re: Flow hood alternatives? [Re: fruit]
    #22420172 - 10/22/15 08:36 PM (8 years, 3 months ago)

I use a big plastic tub placed upside down. First I spray the air in the area Im going to be working in with soapy water. I use to use Lysol but didn't get more contamination when I switched to just soapy water. Wipe my SAB down with ISO, put in on a clean towel, spray the inside with soapy water, wipe my jars, tools etc with iso & put them in the box. Some times I'll spay once more with soapy water. Then wait a couple of min then get to work.


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Offlinefruit

Registered: 10/22/15
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Re: Flow hood alternatives? [Re: dreadhed]
    #22431031 - 10/25/15 09:11 AM (8 years, 3 months ago)

is it not difficult to dry the box out when you have sprayed all that stuff? not much airflow would mean little evap. i personally noticed problems evaping alcohol, of all things in my old glovebox/sab


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Invisibledreadhed
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Re: Flow hood alternatives? [Re: fruit]
    #22433579 - 10/25/15 08:07 PM (8 years, 3 months ago)

I don't dry it out. Just a few mists to bring down and spores, etc that may be in the air.  I don't spray it till water is dripping off the top or anything like that.


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